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Lazzarini, Isis,Detmann, Edenio,Filho, Sebastiao de Campos Valadares,Paulino, Mario Fonseca,Batista, Erick Darlisson,Rufino, Luana Marta de Almeida,Reis, William Lima Santiago dos,Franco, Marcia de Ol Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.8
The objective of this work was to evaluate the effects of supplementation with nitrogen and starch on the nutritional performance of grazing cattle during the rainy season. Five rumen cannulated Nellore steers, averaging 211 kg of body weight (BW), were used. Animals grazed on five signal grass paddocks. Five treatments were evaluated: control (forage only), ruminal supplementation with nitrogen at 1 g of crude protein (CP)/kg BW, ruminal supplementation with starch at 2.5 g/kg BW, supplementation with nitrogen (1 g CP/kg BW) and starch (2.5 g/kg BW), and supplementation with nitrogen (1 g CP/kg BW) and a mixture of corn starch and nitrogenous compounds (2.5 g/kg BW), thereby resulting in an energy part of the supplement with 150 g CP/kg of dry matter (DM). This last treatment was considered an additional treatment. The experiment was carried out according to a $5{\times}5$ Latin square design following a $2{\times}2+1$ factorial arrangement (with or without nitrogen, with or without starch, and the additional treatment). Nitrogen supplementation did not affect (p>0.10) forage intake. Starch supplementation increased (p<0.10) total intake but did not affect (p<0.10) forage intake. There was an interaction between nitrogen and starch (p<0.10) for organic matter digestibility. Organic matter digestibility was increased only by supplying starch and nitrogen together. Nitrogen balance (NB) was increased (p<0.10) by the nitrogen supplementation as well as by starch supplementation. Despite this, even though a significant interaction was not observed (p>0.10), NB obtained with nitrogen plus starch supplementation was greater than NB obtained with either nitrogen or starch exclusive supplementation. Supplementation with starch and nitrogen to beef cattle grazing during the rainy season can possibly improve digestion and nitrogen retention in the animal.
A Robotic System for Soft Tissue Characterization
P,Vacalebri,R,Lazzarini,S,Roccella,F,Vecchi,M,C,Carrozza,M,Zecca,P,Dario 한국과학기술원 인간친화 복지 로봇 시스템 연구센터 2003 International Journal of Assistive Robotics and Me Vol.4 No.2
A miniature robotic system has been designed and fabricated to perform in vivo mechanical characterization of soft tissues. The tissue tester employs a stepper motor, which is controlled using micro-stepping techniques to prevent irregular rotation of motor shaft at low speeds. There is also a load cell to measure the reaction force of the tissue under test. At the end of tests session, data could be post elaborated on a PC with special software that reconstructs the force-displacement curves of the tissues being tested. Position and rate feedback are implemented with a digital encoder to minimize errors in motor control during the evaluation of typical viscoelastic phenomena of biological tissues. Calibration tests indicate that the force-displacement curves of the miniature robotic indenter are consistent with that obtained using an ordinary universal testing machine.
Cristian Conti,Corrado Pedrazzani,Giulia Turri,Eduardo Fernandes,Enrico Lazzarini,Raffaele De Luca,Alessandro Valdegamberi,Andrea Ruzzenente,Alfredo Guglielmi 대한대장항문학회 2021 Annals of Coloproctolgy Vol.37 No.3
Purpose: Laparoscopic complete mesocolic excision (CME) right colectomy is a technically demanding procedure infre- quently employed in Western centers. This retrospective cohort study aims to analyze the safety of laparoscopic CME col- ectomy compared to standard colectomy for right-sided colon cancer in a Western series. Methods: Prospectively collected data from 60 patients who underwent laparoscopic CME right colectomy were com- pared to the ones of 55 patients who underwent laparoscopic standard right colectomy. Results: No differences in clinical characteristics were observed between the CME and standard right colectomy groups. No differences were demonstrated in terms of blood loss (P = 0.060), intraoperative complications (P = 1), conversion rate (P = 0.102), and operative time (P = 0.473). No deaths were observed in either group, while complication rate was 40.0% in the CME and 49.1% in the standard group (P = 0.353). Severe complications occurred in 10.0% vs. 9.1% (P = 0.842), redo surgery in 5.0% vs. 7.3% (P=0.708), and unplanned readmission in 5.0% vs. 5.5% (P=1) after CME and standard colec- tomy, respectively. A significant difference in favor of CME was observed in the total length of specimen (P < 0.001), prox- imal (P=0.018), and distal margins (P=0.037). The number of lymph nodes harvested was significantly higher in the CME group (27 vs. 22, P = 0.037). Conclusion: In Western series, where patients have less favorable clinical characteristics, laparoscopic CME allows to ob- tain better quality surgical specimens and comparable short-term outcomes compared to standard right colectomy.
Marıa de los Angeles Fortis-Barrera,Francisco Javier Alarcon-Aguilar,Araceli Becerril-Garcıa,Jose Luis Eduardo Flores-Saenz,Julio Cesar Almanza-Perez,Mario Garcıa-Lorenzana,Roberto Carlos Lazzarini-Le 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.7
In Central and South American traditional medicine, people use Cecropia obtusifolia Bertol (Cecropiaceae) for the treatment of diabetes mellitus. However, its hypoglycemic action mechanism at pancreatic and liver level has been poorly explored. The present research aimed to establish the influence of the aqueous extract of C. obtusifolia, standardized in its content of chlorogenic acid, on insulin secretion in RINm5F cells and over the liver carbohydrates and lipids metabolism, and to determine concomitantly its hepatoprotective effect on mice with streptozotocin-induced diabetes. In RINm5F cells, concentrations 5, 50, 100, and 200 μg/mL of aqueous extract of C. obtusifolia were used to determine [Ca2+]i and insulin secretion. In an acute study, the extract was administered at doses of 500 mg/kg. In another test (subacute), the extract was daily administrated to diabetic mice (200 mg/kg/day) for 30 days. Blood glucose levels and other biochemical parameters were determined, and a liver histological analysis was performed. In RINm5F cells, C. obtusifolia increased [Ca2+]i and insulin secretion, whereas in diabetic mice exhibited acute and subacute hypoglycemic effects. Daily administration of C. obtusifolia to diabetic mice also increased liver glycogen storage and glycogen synthase levels, without apparent changes in gluconeogenesis. Besides, it increased peroxisome proliferator-activated receptor-α (PPAR-α) and long-chain-fatty-acid-CoA ligase 1 (ACSL-1) expression and reduced triglycerides, transaminases (alanine aminotransferase and aspartate aminotransferase), and collagen fibers, modifying anti-inflammatory (adiponectin and interleukin-10) and inflammatory (tumor necrosis factor-α) cytokines in serum. Therefore, the hypoglycemic effect of C. obtusifolia implicates a dual action, promoting insulin secretion, liver glycogen accumulation, and hepatoprotection by decreasing collagen fibers and inflammatory markers, whereas it improves lipid metabolism, due in part to PPAR-α.