자궁 내 감염 관련 유전자 및 산화 스트레스 관련 유전자와 조기분만의 연관성

유시연 ( Si Yeon You ),김정명 ( Jung Myung Kim ),이경아 ( Kyung A Lee ),박미혜 ( Mi Hye Park ),김영주 ( Young Ju Kim ),전선희 ( Sun Hee Chun ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.7

목적: 조기 분만과 자궁 내 감염 관련 유전자 (IFN-γ, interleukin [IL]-10) 및 산화 스트레스 관련 유전자 (CYP1A1)와의 연관성을 알아보고자 한다. 연구 방법: 2007년 1월부터 2008년 12월까지 이대 목동병원 산부인과에서 산전 검사 및 분만을 시행한 산모 중 37주 이전에 분만한 조산군 164명과 37주 이후에 분만한 대조군 305명을 대상으로 하였으며, 임신 주수 24주 미만, 태아의 선천성 기형, 다태아 분만, 사산의 경우를 제외하였다. 참여자는 입원 시 키, 몸무게 측정 및 혈액 채취를 시행하였고 임신 주수는 최종 월경일 또는 초음파 측정을 통해 계산 되었다. 산모의 혈액에서 IFN-γ, IL-10, CYP1A1 유전자형을 PCR과 SNaPShot method를 사용하여 검사하였으며 카이제곱 검정과 다중 로지스틱 회귀분석을 사용하여 통계분석 하였다. 결과: 조기 분만군과 만삭 분만군 사이에 임신 주수, 태아 체중에 통계적으로 유의한 차이가 있었다(P<0.0001). IFN-γ, IL-10, CYP1A1 유전자형의 빈도와 조기 분만 사이에는 유의한 상관성이 없었다(P>0.05). 결론: 본 연구에서 IFN-γ (874A/T) 및 IL-10 (1082A/G), IL-10 (819C/T), IL-10 (592A/C)와 CYP1A1 (T6235C), CYP1A1 (Ile462val [A/G]) 유전자형의 빈도는 조산과 관련이 없었다. Objective: To investigate the association between preterm birth and cytokine genes (IFN-γ, interleukin (IL)-10) in intrauterine infection and enzyme gene (CYP1A1) in oxidative stress response. Methods: This study involved a case-control study conducted at Ewha Womans University Hospital in Seoul, Korea. Subjects with preterm deliveries (<37 weeks of gestation) and normal controls with term deliveries (≥37 weeks of gestation) were selected from gravidas who had undergone prenatal examinations in the hospital and were followed until infant delivery. The weight, height, and blood samples of each participants were obtained according to standard protocols. We included subjects who gave birth to a singleton infant and had a gestational age between 24 and 42 weeks. Mutiple births, stillbirths, and congenital anomalies were excluded. Finally, 164 gravidas with preterm births and 305 normal controls with term deliveries were enrolled in the present study. Results: Preterm delivery group and term delivery group had significant difference in gestational age and neonatal body weight (P<0.0001). There were no statistically significant association between preterm birth and IFN-γ, IL-10, CYP1A1 genes (P>0.05). Conclusion: In this study, IFN-γ (874A/T), IL-10 (1082A/G), IL-10 (819C/T), IL-10 (592A/C) and CYP1A1 (T6235C), CYP1A1 (Ile462val [A/G]) genes had no significant association with preterm birth.

군체 크기와 세포수 상관관계를 이용한 녹조 유발 남조류의 세포수 산정 방법

유경아 ( Kyung A You ),송미애 ( Mi Ae Song ),변명섭 ( Myeong Seop Byeon ),이혜진 ( Hae Jin Lee ),황순진 ( Soon Jin Hwang ) 한국하천호수학회 2014 생태와 환경 Vol.47 No.4

Harmful Algal Bloom Alert System (HABAS) for drinking water supply is require to fast and accurate count as system monitoring of cyanobacterium occurrence and inducing a response action. We measured correlation between colony size and cell number including genus Anabaena, Aphanizomenon, Microcystis, Oscillatoria which are targeted at HABAS, deducted from standard formula, and suggested calculation method from colony size to the number of cell. We collected cyanobacteria samples at Han River (Paldang reservoir), Nakdong River (Dalseong weir, Changnyeonghaman weir) and Geum River (Gobok reservoir) from August to October, 2013. Also, we studied correlation between colony size and cell number, and calculated regression equation. As a result of correlation of harmful cyanobacteria by genus, Anabaena spp. and Aphanizomenon spp. having trichome showed high correlation coefficients more than 0.93 and Microcystis spp. having colony showed correlation coefficient of 0.76. As a result of correlation of harmful cyanobacteria by species, Anabaena crassa, Aphanizomenon flos-aquae, A. issatschenkoi, Oscillatoria curviceps, O. mougeotii having trichome showed high correlation coefficients from 0.89 to 0.96, and Microcystis aeruginosa, M. wessenbergii, M. viridis having colony showed correlation coefficients from 0.76 to 0.88. Compared with other genus Microcystis relatively showed low correlation because even species and colony size are the same, cell density and cell size are different from Microcystis strains. In this study, using calculated regression might be fast and simple method of cell counting. From now on, we need to secure additional samples, and make a decision to study about other species.

학교행정가의 리더십과 핵심역량에 대한 인식 연구

조경원,한유경,서경혜,조정아,이지은 이화여자대학교 교육과학연구소 2006 교육과학연구 Vol.37 No.1

21세기 지식정보화 사회는 전통적인 학교교육의 변혁을 요구한다. 이에 학교교육 개혁을 성공적으로 이끌어 나아갈 수 있는 교원의 리더십 특히 학교행정가의 리더십이 절실히 요구되고 있다. 그렇다면 지식정보화 사회에서 요구되는 학교행정가의 리더십이란 무엇이며 어떤 역량을 포함하는가? 본 연구는 학교행정가의 리더십과 핵심역량에 대한 선행연구가 학교 현장과는 유리된 채 이론적, 당위적 수준에 그치고 있다는 문제의식에서 출발하여 학교행정가의 리더십을 구성하는 핵심역량이 무엇인가에 대한 교사, 교감, 교장, 행정실장 및 서무부장의 인식을 파악하고 이들 집단의 인식을 비교 분석하는 데 목적을 두었다. 이를 위해 우선 문헌연구를 통해 리더십에 대한 관점과 개념의 변화를 고찰하였고 변혁적 리더십 관점에 기초하여 학교행정가가 갖추어야 할 핵심역량으로 교육적 리더십, 학교 계획 및 조직, 학교교육과정 편성 및 운영, 학교운영, 인적자원 개발, 학교재정 관리, 교육법 이해 및 집행, 학교교육정책 이해, 학교·학부모 및 지역사회 협력, 정보통신기술 활용과 같은 열 가지 역량을 도출하였다. 문헌연구를 통해 도출된 핵심역량에 대한 학교 현장의 관점을 알아보기 위해 초·중·고등학교 교직원을 대상으로 10대 핵심역량의 중요도와 현재 실행정도에 대해 설문조사를 수행하였다. 설문조사에는 144개 학교의 교직원 1,136명이 참여하였다. The purpose of this study is to investigate the perspectives of teachers, vice-principals, and school staff on school administrators' leadership and core abilities. For this purpose, first, we examined the perspectives and concepts of school administrator's leadership and identified 10 core abilities that constitute the school administrator's leadership. Second, we developed a questionnaire to investigate the perspectives of teachers, vice-principals, and school staff on to what extent the 10 core abilities are important and whether their school administrators actually exert these 10 core abilities. Finally, we conducted a survey research, including teachers, vice-principals, and school staff with various years of experiences in elementary and secondary schools in Korea. The findings show that teachers, vice-principals, principals, and school staff consider the 10 core abilities very important for school administrators. However, they perceive that school administrators, in reality, do not exert the 10 core abilities as much as they think these abilities are important. In particular, teachers rated school administrators' leadership relatively low compared to principals, vice-principals, and school staff. The difference between their perception on the importance and exertion of 10 core abilities indicates the need for programs that promote the development of 10 core abilities for school administrators and consequently support the development of school administrator's leadership.

Tight junction protein 1 is regulated by transforming growth factor-β and contributes to cell motility in NSCLC cells

( So Hee Lee ),( A Rome Paek ),( Kyung Sil Yoon ),( Seok Hyun Kim ),( Soo Young Lee ),( Hye Jin You ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.2

Tight junction protein 1 (TJP1), a component of tight junction, has been reported to play a role in protein networks as an adaptor protein, and TJP1 expression is altered during tumor development. Here, we found that TJP1 expression was increased at the RNA and protein levels in TGF-β-stimulated lung cancer cells, A549. SB431542, a type-I TGF-β receptor inhibitor, as well as SB203580, a p38 kinase inhibitor, significantly abrogated the effect of TGF-β on TJP1 expression. Diphenyleneiodonium, an NADPH oxidase inhibitor, also attenuated TJP1 expression in response to TGF-β in lung cancer cells. When TJP1 expression was reduced by shRNA lentiviral particles in A549 cells (A549-sh TJP1), wound healing was much lower than in cells infected with control viral particles. Taken together, these data suggest that TGF-β enhances TJP1 expression, which may play a role beyond structural support in tight junctions during cancer development. [BMB Reports 2015; 48(2): 115-120]

팔당호 퇴적층 남조류의 군집과 남조류 성장잠재성 분석

김용진 ( Yong-jin Kim ),백준수 ( Jun-soo Baek ),윤석제 ( Seok-jea Youn ),김헌년 ( Hun-nyun Kim ),이병천 ( Byoung-cheun Lee ),김귀다 ( Guee Da Kim ),박송은 ( Song Eun Park ),유경아 ( Kyung A You ),이재관 ( Jae-kwan Lee ) 한국물환경학회(구 한국수질보전학회) 2016 한국물환경학회지 Vol.32 No.3

We analyzed cyanobacterial community including akinete, resting cell, and benthic cyanobacteria in sediment of Bukhan, Namhan-River, Paldang-Lake, and Kyeongan-Stream and compared the physicochemical factors for water and pore water. We also performed cyanobacteria growth potential test through incubating sediment. As a result of physicochemical analysis, the concentrations of nutrients were similar for each depth of Bukhan-River. For Namhan-River and Lake Paldang sites, the concentrations of TP and DTN in bottom and deep water had higher levels. DTN in water body composed of NO3-N(73%) while DTN in pore water composed of NH3-N(77.8%). Benthic cyanobacteria in the sediment such as Oscillatoria tenuis, O. limosa, Phormidium tenue, Pseudanabaena limnetica, and Lyngbya sp. were dominant (between 0.0~243.3×103 cells/g, w/w). Cell densities of cyanobacteria in sediment depth of 0~2 cm in most sites were higher compared to those in other depths. The cell density of cyanobacteria in sediment correlated with pH, conductivity, BOD5, TP, DTP, and chl. a. Increased phytoplankton and organic matters were found to be able to inhibit the growth of benthic cyanobacteria. Results of cyanobacteria growth potential test after incubating sediment revealed that harmful cyanobacteria (Anabaena, Aphanizomenon, Microcystis, and Oscillatoria) appeared at 7 days post culturing. Base on these results, the methods used in this study are considered to be able to determine the appearance of harmful cyanobacteria.

Association between genetic variations of vascular endothelial growth factor receptor 2 and atopy in the Korean population

Park, Heung-Woo,Lee, Jong-Eun,Shin, Eun-Soon,Lee, Jae-Young,Bahn, Joon-Woo,Oh, Heung-Bum,Oh, Sun-Young,Cho, Sang-Heon,Moon, Hee-Bum,Min, Kyung-Up,Elias, Jack A.,Kim, You-Young,Kim, Yoon-Keun Elsevier 2006 The journal of allergy and clinical immunology Vol.117 No.4

<P><B>Background</B></P><P>Vascular endothelial growth factor (VEGF) has been suggested to be a key mediator in the development of atopy and T<SUB>H</SUB>2 inflammation.</P><P><B>Objective</B></P><P>We sought to evaluate the effects of variations in the gene coding VEGF receptor (VEGFR) 2 on intermediate phenotypes of asthma in the Korean population.</P><P><B>Methods</B></P><P>A cohort of 2055 children and adolescents responded to a questionnaire concerning asthma symptoms and risk factors and underwent methacholine bronchial challenge and skin tests. The <I>VEGFR2</I> gene, including the promoter area, was sequenced on 24 healthy subjects to discover informative single nucleotide polymorphisms (SNPs; minor allele frequency >2%). After haplotype reconstruction, 4 tagging SNPs (IVS6+54A>G, +889G>A, +1416T>A, and IVS25-92G>A) were scored. These SNPs were also scored in 480 adult asthmatic patients to verify the above genetic association study.</P><P><B>Results</B></P><P>The prevalence of atopy was associated with a single SNP (+889G>A) of VEGFR2 with borderline significance (<I>P</I> = .048; relative risk, 1.13; 95% CI, 1.00-1.28). However, haplotype analysis showed that the atopy prevalence was strongly associated with a haplotype (AGAG) of VEGFR2 (<I>P</I> = .002; relative risk, 1.25; 95% CI, 1.09-1.42). As for airway hyperresponsiveness, neither individual SNPs nor haplotypes were found to be associated. Interestingly, the significant association was also found between atopy and the AGAG haplotype among adult asthmatic patients (<I>P</I> = .008; odds ratio, 1.66; 95% CI, 1.14-2.44).</P><P><B>Conclusions</B></P><P>The present study demonstrated that genetic variations of VEGFR2 are significantly associated with atopy in the Korean population.</P>

A Rare Manifestation of Solitary Primary Bone Lymphoma of the Finger: a Case Report

Yeom, Jeong A,Song, You Seon,Lee, In Sook,Choi, Kyung Un,Kim, Jeung Il Korean Society of Magnetic Resonance in Medicine 2018 Investigative Magnetic Resonance Imaging Vol.22 No.4

Primary extranodal bone lymphoma involving the peripheral extremities is extremely rare. Here, we report a definitive case of diffuse large B-cell lymphoma involving the phalangeal bone of the 3rd finger. Systemic evaluation revealed the lesion as the only site of lymphoma involvement.

중합효소연쇄반응을 통해 확진되고 Atovaquone과 Azithromycin으로 치료한 Human Babesiosis 1예

윤기태,김연아,구남수,김준형,정세진,김홍정,송경호,최유경,신소연,김영근,김명수,박윤선,최준용,송영구,김준명 대한감염학회 2006 감염과 화학요법 Vol.38 No.5

저자들은 국내에서 처음으로 중합효소연쇄반응 검사를 통하여 B. microti에 의한 인체 바베시오시를 진단하였으며, atovaquone과 azithromycin 병합요법을 사용하여 치유한 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Human babesiosis is a tick-borne infectious disease caused by Babesia species. The clinical diagnosis is difficult because of nonspecific symptoms like flu. Rapid diagnosis of human babesiosis is microscopic examination in peripheral blood smear (Giemsa-stain) which reveals characteristic forms of an intracellular quadruplet parasite. But differentiation between Babesia microti and Plasmodium species can be quite difficult because of the morphologic similarity. We experienced a case of human babesiosis. The patient was a 62-year old Korean male who had been in New Jersey, U.S.A for 2 months. We initially diagnosed as malaria infection because the peripheral blood smear revealed intracellular single ring form organism. But the patient was not improved significantly by the treatment with chloroquine regimen. Finally we confirmed human babesiosis by polymerase chain reaction for Babesia microti. We treated the patient successfully with a regimen of atovaquone and azithromycin which has fewer adverse reactions than a regimen of clindamycin and quinine.

OB-45 : Medical treatment with danazol of uterine arteriovenous malformation: a case series of 12 patients

( You Sun Lee ),( Min A Kim ),( Kyung Seo ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

Background: Uterine arteriovenous malformation (AVM) is uncommon and potentially life-threatening condition due to massive vaginal bleeding. Uterine artery embolization is currently the first treatment of choice for uterine AVM and no effective medical treatment is suggested yet. We describe twelve cases that were initially diagnosed with uterine AVM and successfully treated by medical treatment with danazol. Methods: We retrospectively searched patient database for the records of patients with uterine AVM and treated with dazanol in Gangnam Severance Hospital between 2007 and 2013. Twelve patients were analyzed in terms of demographic characteristics, presenting signs and symptoms, diagnostic features, and treatment outcomes. Results: Twelve patients with vaginal bleeding were diagnosed with uterine AVM by the color Doppler vaginal ultrasonography. Three patients went through dilatation and evacuation due to missed abortion before they diagnosed as having uterine AVM. Six patients were postpartum status suspected to have hematometra or retained placenta and four of them were diagnosed after the first trimester termination. The patients took 200mg of danazol twice a day and the median period of use of danazol was 5.7 weeks. Symptom relief and the disappearance of abnormal vasculature in ultrasonography were observed in all patients. Conclusion: Although uterine artery embolization is considered as the main treatment for uterine AVM, medical treatment with danazol may be an alternative safe, non-invasive, and effective treatment in hemodynamically stable patients.

추자도 특집(楸子島 特輯) : 추자도 부근의 지질 환경

유환수 ( Hoan Su You ),유경아 ( Kyung A You ) 한국도서(섬)학회 1998 韓國島嶼硏究 Vol.8 No.1