子宮內膜搔爬術 21例의 鍼麻醉效果에 대한 臨床的 硏究

金在圭,金相佑,李宜暻,金建植,金相佑,南相永 경희대학교 1997 慶熙醫學 Vol.13 No.1

Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.

흉선종 제거후에 발생한 재생 불량성 빈혈 : 증례보고

전원선,이상철,김현정,배상병,김찬규,이남수,박노진,이규택,박성규,홍대식,박희숙,원종호 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

Thymoma is associated with myasthenia gravis, Pure red cell aplasia, and autoimmune diseases such as autoimmune hemolytic anemia, aplastic anemia, and hypogammaglobulinemia. It's association with aplastic anemia is rare and aplastic anemia appearing after surgical removal of thymoma is especially rare. The authors hereby report a case of aplastic anemia occuring in a patient who was diagnosed with thymoma and myasthenia gravis and had the tumor surgically removed. The patient was treated with anti-thymocyte globulin, cyclosporin, and prednisolone, and showed partial remission with hematologic improvements after 12 months.

활동 중심 문학 교수-학습의 문제점과 그 극복방안

김규선,진선희 한국초등국어교육학회 2004 한국초등국어교육 Vol.26 No.-

문학 영역에서 '활동'중심 교수-학습의 의의와 한계를 밝혀 그 문제점을 해결할 수 있는 대안을 살펴보고, 실제 현장의 적용가능성을 탐색해보는 것을 목표로 한다. 활동중심 문학 교수-학습은 여러 가지 의의를 가짐에도 불구하고 구체적인 교수-학습의 상황에서는 자칫 학습독자들에게 진정한 문학 감상이나 체험을 제공하지 못한 채 학습독자들이 활동 자체에 매몰되어 활동은 있었으되 문학 감상은 없는 수업이 되는 경우 등 몇 가지 문제점을 가지고 있다. 이러한 문제점을 극복하고 보다 효율적인 활동중심 문학 교수-학습의 전개를 위한 방안으로, 계획된 활동이 어떤 문학능력을 기르려고 하는 것인지를 교사와 학생모두 교수-학습의 전 과정에서 분명하게 인식하도록 여러 번 되새기는 기회를 갖는 것과 더불어 활동의 결과와 과정에 대한 반성적 사고를 하도록 하는 것이 필요하다. 또 전개되는 학생 활동을 교사의 계획적이고 의도적인 발문에 의해 관리하고 지도하는 것, 교수-학습의 목표에 맞추어 활동들의 시간을 적절하게 배분하는 교사의 능력, 활동의 방향을 조절하고 통제할 수 있는 간단한 메타적 조작과정을 병행하는 것 등을 활동중심 문학 교수-학습이 가진 문제점을 극복하는 각명(inscription)장치로 제시하였다. This study set out to investigate 'activity-based' teaching-learning methods adopted in literature in terms of its implications and limitations, to search for the alternatives to solve the problems, and to examine the possibility of their application to the field. Despite the many implications, the activity-based literature teaching-learning method does have some limitations such as failing to provide true literary appreciation or experiences to the students under specific teaching-learning situations and letting the students be lost themselves in the activity itself with no literary appreciation. The followings are the plans to overcome those limitations and to make the activity-based literature teaching-learning method more effective: first, they should remind themselves of what literary capacities are targeted in the planned activity over and over again so that both the students and the teachers can have a clear understanding of them throughout the teaching-learning process. They also need to do reflective thinking on the results and process of the activity. The teachers need to use their planned and intentional postscripts to monitor and guide the student activities, to increase their ability to allocate time to each activity properly according to the goals of teaching-learning, and to introduce a simple meta-manipulation process to adjust and control the directions of the activities in order to improve the effects of the activity-based literature teaching-learning method.

백서의 절치부 기능상실이 악관절 성장에 미치는 영향에 관한 연구

김선희,김선현,양규호 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.1

As a secondary carilage, the condylar cartilage of the mandible is known to be responsive to extrinsic stimuli of local biomechanical origin. The purpose of this study was to study the effects of the mandibular joint to the loss of incisal function in the rat. Seventy five 21-day-old male rats(Sprague-Dawley strain) were divided into three groups and each groups were subdivided into 1,3,7,14, and 21 days groups. In incisor clip group, both the maxillary and the mandibular incisor teeth were trimmed bilaterally to the level of the gingiva every other day. The soft diet group was fed a soft diet consisting of mush or thick soup. The third group of untreated rats fed normal rat pellets constituted the control group. At 90 minutes prior to sacrifice, all rats were injected intravenously with 1 ? Ci/g body weight of [³H]-thymidine (Amersham UK,specfic activity=2 Ci/m mol). The animals were killed at 1,3,5,7,14,21 days and the TMJs were prepared for the histologic,histomorphometric and biochemical analysis. The results were as follows: 1.Both the incisor clip and soft diet groups exhibited reduced size and density of bony trabeculae underlying the condylar cartilage and retarded osteogenesis. 2.The thickness of the prechondrobiasic layer of the condylar cartilage was increased for the experimental period in all groups. 3.The thickness of prechondoblastic layer of superior portion of the condylar cartilage in both experimental group and that of posterior and posterosuperior portion in only incisor clip group were significantly reduced compared to the control group. 4.For all periods, incorporation of ³H-thymidine was signifiactly decreased compared to control group in the incisor clip group, and soft diet group exhibited intermediate in corporation value between the control and incisor clip group. These results suggested that the loss of incisal function by incisor clip and physical consistency of diet may have an effect on growth of the mandibular joint.

유치열 아동의 교합양상및 측모에 관한 역학적 연구

김선미,양규호,김선희 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.1

The occlusal patterns and profiles of the primary dentition were studied by oral examination from 3 to 6 year-old children (100 boys, 100 girls) in Kwangju city. The results were as follows : 1. In sagittal primary second molar relationship, mesial step(48%) and flush terminal plane relationship (47.5%) were prevalent. 2. In profile view, straight profile (57%) was prevalent. 3. In comparison of relationship between primary second molar relationship and profile, Class 1 pattern-straight profile (31 boys, 32 girls) was prevalent.

鍼灸科 領域에 있어서의 腰脚痛에 對한 臨床的 觀察

金在圭,崔翊善,朴東錫,安秉哲 慶熙大學校韓醫科大學韓醫學硏究所 1980 慶熙韓醫大論文集 Vol.3 No.-

Seed Germination in the Vitaceae : Ⅰ. Responses of Parthenocissus tricuspidata Seeds to Stratification and Temperature

金善圭,李大鎭,金吉龍,崔東龍,李英喆 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.1

Influence of moist chilling at 3°C and subsequent germination temperature on seed germination of Parthenocissus tricuspidata (Sieb. & Zucc.) Planch. Was studied. 1.Seed germination of P. tricuspidata increased between 20° and 25°C by prolonged chilling of 8 to 12 weeks. 2.Percent seed germination was higher at 20°C than 25°C, but germination rate was higher at 25°C. 3.Length of time for germination(LTG) and germination span (GS) in days were decreased by prolonged chilling. LTG and GS were also lower at 25°C than 20°C. 4.P. tricuspidata seemed to be a dark germinating species.

한국 성장기 아동의 Ⅲ급 부정교합 양상에 관한 두부방사선 계측학적 연구

양규호,김선희 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.1

This study was performed to analyse the pattern of the Class Ⅲ malocclusion in Korean growing children. The cephlometric radiograms were taken from the growing children at 8 to 12 years old, then 11 items were statistically analysed. The results of this study were as follows : 1. As compared 11 items between Class Ⅲ malocclusion and normal occlusion, there was significant difference on SNA angle, Maxillary incisor to point A(mm), Maxillary incisor-NA line(angle), Maxillary incisor to NA line(mm), IMPA, Facial angle and Pog to Nasion Perpendicular(mm) between Class Ⅲ malocclusion and onrmal occlusion. 2. The most common Class Ⅲ malocclusion pattern was found that the normal maxilla and madible followed by normal maxilla and prognathic mendible. 3. In vertical component of Class Ⅲ malocclusion, subjects with long lower facial height were 54% of the total but with short lower facial height were 10%.

설하선에서 기인한 경부하마종 치험 2례

김일규,김영렬,김종철,유선열,문승옥 대한악안면성형외과학회 1986 대한악안면성형외과학회지 Vol.8 No.1