Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

면외변위의 정량적 계측을 위한 ESPI와 Shearography의 비교

최정구,김경석,장호섭,정성욱 朝鮮大學校 機械技術硏究所 2005 機械技術硏究 Vol.8 No.2

Electronic Speckle Pattern Interfrometry is a common method for measuring out-of-plane displacement and in-plane displacement and applied for vibration analysis and strain/stress analysis. However, ESPI is sensitive to environmental disturbance, which provide the limitation of industrial application. On the other hand, Shearography based on shearing interferometer can directly measure the first derivative of out-of-plane displacement, which is insensitive to vibration disturbance. This paper proposes the out-of-plane displacement extraction technique from results of Shearography by numerical processing and measurement results of ESPI and Shearoraphy are compared quantitatively.

氣化器붙이 機關의 共給燃料 氣化率에 關한 基礎硏究

崔鍾宇,朴涇錫 慶熙大學校 1985 論文集 Vol.14 No.-

This study has been made to observe the effect of vaporization of injected fuel under the several important factors on the carburetor for gasoline engine. To measure the vaporization rate of mixture in the carburetor, the key point of consideration for divising the cyclone which is devised to be able to separated injected fuel with drop diameter 10-30㎛. By using a transparent tube type model of the carburetor, like one of conventional gasoline engines, this experiment has been performed. Results of the study are summarized as follow. 1. In the conditions of this study, Ve=12.6 - 50.4㎧, θ=30 - 90, t = 25.℃ - 50℃, the vaporization rate is 10-50% 2. The vaporization rate increase in proportion to decreasing of throttle valve angle, to rising air temperature and growing air - fuel ratio. 3. When air velocity is increased, the injected fuel flows with thin thickness of liquid film on the inner wall of intake pipe. As air temperature is increased, it flows with more thin.

추적조사를 중심으로 한 腰椎間板脫出症에 대한 蜈蚣藥鍼의 臨床的 硏究

최회강,소기숙,고강훈,박소영,김성남,이종덕,원경숙,문형철,김성철 大韓鍼灸學會 2003 대한침구의학회지 Vol.20 No.3

Objective : This study is performed for the purpose of examining the efficacy of the scolopendrid(Scolopendra morsitans L) which has been to low back pain as a folk remedy. Methods & Results : Seventy patints with H.I.V.D. treated with the scolopendrid aquacupuncture and examined 4 tests; D.I.T.I., physical test, Oswestry disability index, and self-conscious pain rate were studied. And we follow up after discharge, we evaluated the present state and recovery period. Or we evaluated by herniated type and duration of onset. There was 58~97% of efficacy rate in each tests. Moreover, statistically valuable change in all 7D.I.T.I. points and in disability index and in self-conscious pain rate was showed. Conclusion : The scolopendrid aquacupuncture is possible to be effective terating the H.I.V.D patients. so we suggest the possibility using this new remedy to the H.I.V.D. patients.

新羅의 官制 改革에 관한 考擦

崔敬淑 부산외국어대학 1983 논문집 Vol.1 No.-

In the Jikwanji of Samkuksaki(三國史記 職官志)well illustrates the continous development of government organization throughout the Silla. This change goes father than to record the historical changes of the offical titles. It is significant in that it demonstrates the political and social variations which influenced the shift from a tribal state to an ancient autocratic state. Therefore an attempt is made here to survey the mutating process of Silla through the reformation of government organizations. The structure of this paper is as follows: first of all, the process of adding new institutions in the early Silla government system will be discussed. Secondly, the social and political influence which affected the restoration to the old system in the social and political influence which affected the restoration to the old system in the reign of king Hyegong(惠恭), after the 17 year excution of the over all reformation in the region of king kyongdok(景德) in the latter part of the middle period(中代), And since the historical documents proves that this restored old system have been developed to be the widely reformed in the reign of king kyongdok(景德), the cause of this development will be studied in connection with the power conflict between the autocrat and the aristocratic class and with the system of in the late period(下代).

燃料噴流와 周圍氣體와의 混合過程에 關한 硏究(Ⅰ) : 予混合氣形成의 變動濃度測定및 流動可視化 Concentration Measurements and Visualization of the Premixed Mixture Formation

朴涇錫,崔鍾宇 慶熙大學校 1986 論文集 Vol.15 No.-

This paper presents a fundamental study on the mixing process of the premixed mixture. Nonuniformity of fuel and air mixture influences the combustion characteristics and exhaust emissions of the premixed type combustor. The object of this study is to express the mixture uniformity statistically by means of measurement of the fluctuating concentration field with the high response hot wire concentration probe and to visualize the premixed mixture formation by smoke wire methods.

땅비싸리와 좀땅비싸리의 형태적변이 및 분류

조숙경,최병희,김주환 대전대학교 이과대학 기초과학연구소 1997 自然科學 Vol.- No.-

동북아시아에 분포하는 땅비싸리와 좀땅비싸리의 분류학적 한계를 규명하기 위하여 형태적 변이와 염색체수가 조사되었다. 땅비싸리의 이명으로 취급되어 왔던 좀땅비싸리는 소엽의 모용, 꽃의 크기, 화분형태, 지리적 분포 등에 있어 땅비싸리와 뚜렷한 차이를 보였으며, 또한 염색체수가 4배체(2n=32)로 나타나, 2배체(2n=16)인 땅비싸리와 차이를 보였다. 땅비싸리와 좀땅비싸리 11 집단을 대상으로 19개의 외부형태 형질에 대한 주성분분석을 실시한 결과 두 종은 제1주성분에 의해 뚜렷이 나누어 졌는데, 전체분산에 대한 제1주성분의 기여율은 63.2%이었으며, 제1주성분은 소엽 표면의 털과 화서의 길이 등과 높은 상관성을 보였다. 이러한 결과로부터 좀땅비싸리는 땅비싸리와는 구별되어 독립된 종으로 처리되었다. 한편 큰땅비싸리는 땅비싸리의 변이의 한 형태로 판단되므로 땅비싸리의 이명으로 처리되었다. 각 종의 지리적 분포에 대한 분류학적 의의가 염색체수 변이 등과 비교논의 되었다. The morphological analysis and cytological study of Indigofera kirilowii Maxim. and Indigofera korean Ohwi were undertaken to assess the numbers and ranks of taxa that usefully could be recognized. I, koreana, previously recognized as I. kirilowiii, was separated from I. kirilowii in hairiness on leaflets, flower size, pollen exine sculptures, geographical distribution and chromasome number. Chromosome count of 2n=32 (tetraploidy) from I. koreana was obtained for the first time. The results of principal components analysis (PCA) on a matrix of 11 populations of Korea by 19 morphological characters indicated that two species could be recognized by the first principal component, contribution of which for the total variance is 63.2%. Our results supported that I. koreana could be clearly distinguished from I. kirilowii. Since the quantitative differences between the two varieties, I. kirilowii var. coreana and var. Kirilowii, were smaller, a nomenclatural synonymy was developed for them, ie, var. kirilowii.

남한강 상류수계 박대천의 어류상

박경서,문운기,홍영표,최신석 忠南大學校 環境問題硏究所 2001 環境硏究 Vol.19 No.-

남한강 상류 수계, 박대천 일대를 대상으로 2001년 4월부터 9월까지 총 11개 지점을 대상으로 조사하였으며, 종조성, 어류상과 군집 구조를 분석하였다. 총 7과 25속 33종으로 1570개체가 조사 기간동안 확인되었다. 전체 어류군집 중, 잉어과 어류가 24종으로 전체의 72.7%와 1531개체로 전체의 97.5%를 차지하였다. 전체 종에 대해 0.2%보다 적은 종은 L. andersoni, G. brevibraba, A. koreensis, C. rotundicaudata와 S. microdorsalis를 포함한 11종이었다. 박대천 수계에서 우점종은 상대풍부도가 28.66%로 Zacco temmincki가 우점하였고, Zacco platypus (28.28%)와 C. splendidus (12.55%)가 아우점하였다. 박대천의 각 지류에서, Zacco temmincki는 6개 지점에서, Zacco temmincki는 5개 지점에서 우점하였다. 박대천 수계로부터 총 33종 중 6과 14속 18종(54.5%)이 한국고유종이었다. 박대천에서 확인된 어류의 전체 군집 구조를 분석한 결과, 우점도 3.766, 다양도 0.903, 풍부도 10.012, 그리고 균등도는 0.594이었다. Sampes of the Bakdae-Cheon Stream, the upper Namhan-Gang River of Korea were collected at 11 stations from April to September 2001, and analyzed for the species composition, fish fauna, and species diversity. A total of 33 species (1570 individuals) and 25 genera belonging to seven families were collected during the study period. Cyprinid fishes occupied 72.7% of total 24 species and 97.5% of total 1531 individuals, respectively. The rare species which relative abundance is less than 0.2% of all were 1531 individuals, respectively. The rare species which relative abundance is less than 0.2% of all were about 11 species including Liobagrus andersoni, Gobiobotia brevibraba, Acheilognathus koreensis, Cobitis rotundicaudata, and Silurus microdorsalis. Dominant species in the Bakdae-cheon Stream was Zacco temmincki (relative abundance 28.66%), and subdominant species were Zacco platypus (28.28%), and Coreoleuciscus splendidus (12.55%). In each trivutaries of the Bakdae-cheon Stream, Z. temmincki was dominant species at six stations, and Z. platypus was dominant at five stations. Eighteen Species and 14 genera belonging to six families (51.5%) of total 33 species from the Bakdae-cheon Stream were well known as Korean endemic species. The species dominance (λ), diversity (H'), richness (RI), and evenness index (EI) of the fish community were 3.766, 0.903, 10.012, and 0.594, respectively.

ICPCVD 방법에 의한 Nano-pore형 저유전 박막의 형성

오경숙,최치규,이광만 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

Si-O-C-H composite films were deposited using a ratio frequency inductively coupled plasma chemical vapor deposition(ICPCVD) system with a BTMSM(bis trimethyl silymethane) precursor and oxygen gases. FTIR spectroscopy and XPS were used to investigate the bonding configurations such as Si-O-Si, Si-O-C and Si-CH_3 bonds in the films. From the changes in the FTIR and XPS spectra between the as-deposited and annealed film, we infer that the attachment of the Si-O-Si ring link with CH_3 groups in useful for forming a nano-sized pore in the film. It can be explained to obtain a low dielectric constant(k= 2.5)

항공우주용 복합재튜브의 충격압궤특성에 관한 연구

김영남,최효석,기경서,김기형,양인영 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1

본 연구의 목적은 준정적 및 충격실험에 대한 CFRP 박육부재의 에너지흡수특성에 관하여 고찰하고자 한다. 준정적실험은 신강좌굴실험기를, 충격실험은 수직식 충격압궤실험장치를 사용하였으며, 총 178개의 CFRP 박육부재에 대하여 준정적 및 충격압궤실험을 수행하였다. CFRP 원형관의 압궤실험시 그 응답특성은 매우 복잡하며, 압궤과정중 상이한 역학적 상호관계에 의존하게 된다. 본 연구에서는 다양한 적층조건을 갖는 박육부재의 압궤특성과 흡수에너지에 관하여 실험후 고찰하였다. 그 결과 CFRP 박육부재의 에너지 흡수 특성은 초기결함(Trigger)과 계면수 등에 관한 다양한 함수로 나타났으며, 일반적으로 CT-타입의 CFRP 박육부재의 에너지 흡수특성이 다른 박육부재에 비해 가장 높음을 알수 있었다. The purpose of this study is to investigate on the energy absorption characteristics of CFRP (Carbon Fiber Reinforced Plastics) tubes on quasi-static and impact tests. Quasi-static and impact compression tests were performed on 178 tubes. Static compression tests have been carried out using the static testing machine(Shin-gang buckling testing machine) and impact compression tests have been carried out using the vertical crushing testing machine. When such tubes are subjected to crushing loads, the response is complex and depends on the interaction between the different mechanisms that control the crushing process. The collapse characteristics and energy absorption of a variety of tubes have been examined. The results varied significantly as a function of trigger and interlaminar number. In general, the CT-type tubes absorbed more energy than the others.