人蔘의 育種 및 栽培年限 短縮에 關한 基礎硏究 : 第1報 新芽의 體眠打破에 미치는 植物生長調節物質의 影響 Effect of some growth regulators on the dormancy breaking of new buds

崔京求,金鎭淇,黃種奎 全北大學校 1978 論文集 Vol.20 No.-

This study was carried out to investigate the effect of some growth regulators i.e., gibberellic acid (GA), benzyladenine (BA) and abscisic acid (ABA) on the dormancy breaking of new buds in 2 year-old Ginseng plants. Materials were sampled in the middle of October and their dormancy was checked under the favorable conditions for germination of bud for a month. They were treated for 24 hours with the following growth regulators; (1) GA, BA and ABA at the concentrations of 50 and 100 ppm, and (2) the combination of GA and BA, GA and ABA, and BA and ABA at the concentrations of 50 ppm, respectively. It was found that the application of GA was effective in dormancy breaking of new buds and seemed to elongate stem, flower stalk and petiole but degenerated the majority of flower stalk in the early stage and also accelerated the formation of the adventitious root. By the application of GA, poor development of color was observed in leaf and stem in the early stage. The leaf began to regain green color with leaf maturation but the stem did not gain purple color. The applications of BA and ABA combined with GA showed almost the same effect in dormacy breaking as GA application. In these cases color development of stem and leaf was good cmpared with that of GA application. Even with some side-effects such as elongation and poor color development of stem and leaf, GA might be applied for dormancy breaking of new buds substituting for the low temperature in winter.

人蔘種子에 있어서 後熟過程中 內生 Hormone의 變化

崔京求,張熙振,柳南熙 全北大學校 1994 論文集 Vol.37 No.-

This study was conducted to investigate the seasonal changes of inhibitors during after-ripening of the ginseng seeds. The bioassays of lettuce seed germination rate to the water and chlorogorm soluble fraction extracted from ginseng seeds during stratification were applied. The obtained results were summarized as follows: 1. Three kinds of germination inhibitors were detected in the water soluble fraction of the after-ripening seeds, and the activity of inhibitors was more increased at the Rf 0.1 zone than that of the Rf 0.4-0.5 and 0.8-1.0 zone. 2. Chroloform soluble fraction showed the similar tendency to the water soluble fraction, and promoter was recognized at the Rf 0.1-0.2 zone in the seeds of 90th day after stratification. The inhibitor of the Rf 0.1-0.2 zone increased in accordance with stratification, and that of the Rf 0.9-1.0 zone decreased slightly. 3. On the whole, the physiological activities of three kinds of germination inhibitors from the water soluble fraction showed more increase than those of the chloroform soluble fraction. 4. The water and chloroform soluble 'faction from the embryos of ginseng seeds during stratification showed two kinds of germination ingibitors at the Rf 0.4-0.5 and 0.9-1.0 zones, and water soluble fraction from the endosperms of ginseng seeds showed three kinds of inhibitors at the Rf 0.1-0.2. 0.4-0.5 and 0.7-1.0 zones.

벼 未熟種子의 休眠과 發芽抑制物質에 對하여

崔京求,李王休 全北大學校 1980 論文集 Vol.22 No.-

The present study was carried out to clarify the inducing factors of seed dormancy of 12 newly improved rice varieties by checking the effect of hull and germination inhibitors on seed germination of 30 day-old rice seeds. The obtained results were as follows; 1. Hulled or half-hulled rice seeds showed remarkably high percentage of germination and rapid germination speed. Therefore, the dormancy of immature rice seed seemed to be mainly due to its hull. 2. Soaking seeds in water at 30℃ had an effect on breaking dormancy and the effect tended to be decreased in inverse proportion to the degree of seed dormancy. 3. Through bioassay with lettuce and rice seed germination, the existence of germination inhibitors which leached into water from rice seeds was recognized. In general, high dormant rice varieties tended to have less water soluble inhibitors than low ones. 4. The inhibitors in rice seeds were detected in the zones of Rf 0.1-0.3 and Rf 0.8-1.0 even though the patterns of paper-chromatogram on inhibitors in seeds differed with varieties. 5. The amount of germination inhibitors in seeds was closely but not completely related with the degree of seed dormancy. Therefore, it may be reasonable to evaluate the degree of immature rice seed dormancy with the amount of inhibitors in the seed and that of leaching inhibitors from it. 6. In some varieties such as Iri 332, Milyang 23, and Milyand 29, the seed dormancy was also due to the physiological immature of embryo as well as the effect of hull and inhibitors. 7. Considering the above conditions, the tested 12 varieties may be grouped as follows; a. Low dormant varieties: Tongil, Josaengtongil, Suweon 264, Tongilchal, Milyang 21. b. Medium dormant varieties: Iri 326, Iri 327. c. High dormant varieties: Iri 332, Yushin, Milyang 23, Milyang 29, Milyang 30.

옥수수 播種期와 地上部形質에 관한 硏究

金鎭淇,崔京求,白南赫,陳星桂 全北大學校 1982 論文集 Vol.24 No.-

全北 平野地인 裡里地方에서 옥수수의 播種期와 地上部生育 및 種實收量과의 관계를 調査하여 다음과 같은 결과를 얻었다. 1. 播種期의 早晩이 莖葉의 生育 및 種實收量에 크게 영향을 끼치며 播種이 늦어질수록 出雄, 出絲, 生育日數가 짧아지고 地上部生育은 떨어지는 경향이다. 2. 莖葉生育은 4月 20日 播種을 基點으로, 種實收量은 5月 5日 播種을 基點으로 各各 減少되었다. 3. 地上部諸形質과 種實收量간에는 高度의 正의 相關이 인정되며 種實增收를 위하여는 莖葉生育은 크게 할 필요가 있다. Some results were gained through the experiment that was carried out with a single cross cultivar, Suweon 19, of maize (Zea mays L.) in Iri, the Jeonbug plain area, in 1980, to check relationships between growth characters at different sowing dates. 1. The foliage growth and grain yield were greatly influenced with planting season. The number of days to tasseling, silking, or maturing shortened and the growth of maize decreased as sowing was delayed. 2. The threshold sowing date for foliage production seemed to be April 20 and that for grain yield, May 5. 3. Highly significant positive correlation existed between vegetative growth and grain yield. This may mean that increasing vegetative growth is prerequisite to better grain yield.

新品種 밀 "早光"의 沓裏作 播種期 및 播種量에 관한 硏究

黃鍾奎,崔京求,陳星桂 全北大學校 1979 論文集 Vol.21 No.-

This experiment was carried out under different seeding dates(October 10, Oct. 20 or November 10) and seeding rates (20, 30, 35 ℓ 10a) from 1976 to 1977 in Iri in order to study the suitable seeding date and seeding rate for Jokwang, the newly developed wheat variety, in double cropping paddy field. 1. Number of days to seedling emergence both on Oct. 20 and Oct. 30 were 14 days, but that of Nov. 10 was prolonged due to comparatively low temperature and about 37 days. 2. The heading date and the maturing date seemed to be shortened by 1 or 2 days in accordance with the earlier seeding date and the larger amount of seeding. 3. Number of panicles per square meter seeded on Oct. 20 was 506 which marked the most among the three seeding dates. 30ℓ 10a plot showed the most in panicle number among three seeding rates. 4. Number of grains per panicles was increased by 1 or 2 grains with hastened seeding date, but it was decreased by 2 or 3 grains with the increase in seeding rate. 5. The weight of 1,000 grains of the plots both seeded on Oct. 20 and Oct. 30 was about 38.0 g. and that of Nov. 10 was 35.0g. On the other hand, there were almost negligible differences in 1,000 grains weight between seeding rates at the same seeding date. 6. The yield of the plot seeded on Oct. 20 was 359 ㎏ 10a which marked the highest among the three seeding dates. Among seeding rates, 30 ℓ/10a plot showed the highest yield of 351 ㎏ 10a. However, the highest yield among all treatments, seeding dates and seeding rates, was 382 ㎏ 10a which was obtained from the plot seeded on Oct. 20 with the seeding rate of 30 ℓ/10a. 7. Therefore, it was considered that the suitable seeding date for Jokwang, the newly developed wheat variety, in double cropping paddy field in Iri area was Oct. 20 and its seeding rate was 30ℓ/10a.

麥酒麥의 (1-3,1-4)-β-glucanase 遺傳子의 構造와 發現 樣狀

유남희,윤성중,최경구,김제환,박충웅 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

禾穀類 種子 發芽時 호분층과 ??組織의 細胞壁에 多量 含有되어 있는 (1-3, 1-4)-β-glucan을 分解하여 種子의 發芽에 必要한 加水分解酵素의 分泌 및 擴散 浸透를 돕는다. 本 硏究에서는 國內 麥酒麥의 (1-3, 1-4)-β-glucanase 遺傳子의 構造 및 發現 에 대한 基礎 情報를 얻기 위하여 生化學的 및 分子生物學的 方法을 利用하여 發芽種子 및 어린 잎에서의 酵素活性과 遺傳子의 發芽樣狀 그리고 遺傳子의 構造 等을 調査하였다. 麥酒麥 發芽種子中 (1-3, 1-4)-β-glucanase 活性은 發芽 7日째에 제일 높았다. (1-3, 1-4)-β-glucanase의 活性測定에 使用한 (1-3, 1-4)-β-glucan이 (1-4)-β-glucanase에 의해 分解될 可能性이 있으므로 (1-4)-β-glucanase의 活性도 測定하였는데 (1-4)-β-glucanase의 活性이 매우 낮게 나타나서(1-4)-β-glucanase가 (1-3, 1-4)-β-glucanase을 分解하여 (1-3, 1-4)-β-glucanse의 活性에 影響을 미쳤을 可能性이 매우 낮았다. 麥酒麥의 (1-3, 1-4)-β-glucanase 遺傳子 構造를 PCR을 利用하여 調査하였던바 國內 麥酒麥의 (1-3, 1-4)-β-glucanase 遺傳子 構造는 外國의 다른 品種들이나 귀리의 遺傳子 構造와 대단히 類似하였다. 酵素活性 測定과 귀리의 (1-3, 1-4)-β-glucanase cDNA clone을 ?識子로 利用한 보리의 mRNA 分析에 의해서 發芽傳子 및 어린 잎에서 (1-3, 1-4)-β-glucanase가 種子發芽 以外에도 生育中인 組織細胞의 伸張과 發育 및 β-glucan 再利用에 泌要한 β-glucan 代謝에 關與하고 있음을 示唆하는 結果라 생각된다. (1-3, 1-4)-β-glucans hydrolysis of cell wall (1-3, 1-4)-β-glucanas accompanies degradation of endosperm cell walls during cereal seed germination. To investigate (1-3, 1-4)-β-glucanase expression pattern in Korean malting barley, (1-3, 1-4)-β-glucanase activity, gene structure and mRNA expression patterns werre examined. (1-3, 1-4)-β-glucanase activity increased as germination proceeded and the highest enzyme activity was detected in seeds germinated for 7 days. Because (1-4)-β-glucanase have a potential to degrade (1-3, 1-4)-β-glucans, (1-4)-β-glucanase activity was also determined. (1-4)-β-glucanase activity, however, was very low minimizing possibility of (1-4)-β-glucanase action on (1-3, 1-4)-β-glucan substrate. PCR technique was employed to investigate (1-3, 1-4)-β-glucanase gene structure. The amplification product of the expected size from the targeted sites of Dusan #29 indicated that (1-3, 1-4)-β-glucanase systems were conserved in different barley varieties and even in oats. (1-3, 1-4)-β-glucanase activity and mRNAs were detected in young leaves as well as in germinating seeds. This may suggest that (1-3, 1-4)-β-glucanase are also involved in β-glucan metabolism required for cell elongation and β-glucan reutilization during leaf growth and developmetn.

Expression of Peanut Resveratrol Synthase Gene in Response to Hormones and Stresses

Kyeong Gu Choi,Song Joong Yun,Nam Hee Yoo,So Hyeon Baek,Myeong Ryeol Park 한국육종학회 2001 한국육종학회 학술발표회 발표요지 Vol.2001 No.5

The p38 MAPK pathway is critical for 5,5&vprime;-dibromodiindolylmethane-induced apoptosis to prevent oral squamous carcinoma cells

Choi, Kyeong-Hee,Kim, Hyung-Kook,Kim, Jun-Hee,Choi, Eun-Sun,Shin, Ji-Ae,Lee, Syng-Ook,Chintharlapalli, Sudhakar,Safe, Stephen,Abdelrahim, Maen,Kong, Gu,Choi, Hong Seok,Jung, Ji-Youn,Cho, Hyun-Tae,Cho, Lippincott Williams Wilkins, Inc. 2010 EUROPEAN JOURNAL OF CANCER PREVENTION Vol.19 No.2

Cruciferous vegetables contain isothiocyanates including diindolylmethane (DIM) that exhibit cancer chemopreventive effects. We developed a series of synthetic ring-substituted DIM analogs including 5,5&vprime;-dibromoDIM that exhibited better inhibitory activity in breast and colon cancer cells than DIM. In this study, we investigated whether 5,5&vprime;-dibromoDIM inhibits the proliferation of KB and YD-10B oral squamous carcinoma cell lines. 5,5&vprime;-dibromoDIM decreased the cell survival and inhibited the growth of oral cancer cells. Exposure of KB and YD-10B cells to 5,5&vprime;-dibromoDIM induced caspase-dependent apoptosis evidenced by poly-ADP ribose polymerase cleavage, accumulation of sub-G1 population, and nuclear condensation and fragmentation. In addition, apoptotic cell death was correlated with damage to the mitochondrial membrane potential through a decrease in the level of Bcl-2 protein expression. Mechanistic studies showed that mitochondria-dependent apoptosis induced by 5,5&vprime;-dibromoDIM was mediated by the p38 mitogen-activated protein kinase pathway but not the ERK1/2 and JNK pathway. These results highlight 5,5&vprime;-dibromoDIM as an important chemopreventive agent for the clinical treatment of oral cancer through the p38 mitogen-activated protein kinase pathway.

[Poster] Case Report: Noncalculous hemorrhagic cholecystitis

Kyeong Woon Choi,Sung Woo Hong,Yeo Gu Jang,Woo Yeong Lee,Byeong Mo Lee,Hyeok Sang Lee,Yun Kyeong Kang 대한외과학회 2011 대한외과학회 학술대회 초록집 Vol.2011 No.11

수도의 생장반응에 미치는 염분농도의 영향

최경구(Kyeong Gu Choi),김재수(Jae Soo Kim),장영남(Young Nam Chang),이종영(Jong Young Lee) 전북대학교 농업과학기술연구소 1987 농업생명과학연구 Vol.18 No.-