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RISS 인기검색어
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- 저자 : Kwideok Park (검색결과 40 건)
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Park, Kwideok,Huang, Jinsong,Azar, Frederick,Jin, Ri L.,Min, Byoung-Hyun,Han, Dong K.,Hasty, Karen Blackwell Publishing Inc 2006 Artificial Organs Vol.30 No.8
<P>Abstract: </P><P>This study introduces an implantable scaffold-free (SF) cartilage tissue construct that is composed of chondrocytes and their self-produced extracellular matrix (ECM). Chondrocytes were isolated from the articular cartilages from knees of domestic pigs (2-week old) and monolayer-cultured for 3–4 days in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum and 50 µg/mL of ascorbic acid. Briefly treated with 0.25% trypsin–ethylenediaminetetraacetic acid (EDTA), an intact chondrocytes/ECM membrane, as a cell sheet was released from the plate bottom and subsequently centrifuged into a pellet-type construct. Each was grown in vitro for up to 5 weeks and subjected to various assays at different time points (1, 7, 14, 21, and 35 days). For in vivo implantation, full-thickness defects (<I>n</I> = 4) were manually created on the femoro-patellar groove of the left porcine knee and 1-week-cultured SF construct was implanted as an allograft for a month. One defect (♯1) was an empty control and the remaining three received different recipes; construct only (♯2) or 0.25% trypsin/EDTA-treated first and then construct and collagen gel (♯3) or construct and collagen gel (♯4). While the total cell numbers significantly increased by 2 weeks and then remained stable, cell viability stayed in the mid-70% range through the entire culture period. Biochemical assay found continuous glycosaminoglycan (GAG) accumulation. Histology exhibited that cell distribution was even in the construct and GAG intensity became stronger and uniform with time. Real-time reverse transcription polymerase chain reaction (RT-PCR) results showed that phenotypic stability peaked at 2 weeks, which was arable to that of freshly isolated chondrocytes. Upon analysis of the retrieved implants, some promising results were witnessed in the defects (♯3) retaining not only their intact mass but also chondrocytic morphology with lacuna formation. </P>
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Park, Min Hee,Subbiah, Ramesh,Kwon, Min Jung,Kim, Woo Jun,Kim, Sang Heon,Park, Kwideok,Lee, Kangwon Elsevier 2018 Carbohydrate polymers Vol.202 No.-
<P><B>Abstract</B></P> <P>Alginate (Alg) hydrogels, the most popular natural biomaterials, mimic the extracellular matrix (ECM) microenvironment and offer potential biomedical applications. Despite their excellent properties such as biocompatibility, hydrophilicity and ionic crosslinking, the absence of an intrinsic cell adhesion domain lessens their cell-carrier applications in tissue engineering. Herein, we suggest a three-dimensional (3D) cell culture system by integrating Alg hydrogel and fibroblast-derived matrix (FDM). FDM including cell-adhesion motifs, signaling, and physico-mechanical cues is prepared by the decellularization process by avoiding unfavorable chemical reactions. This cues-integrated-biomaterials (CiB) 3D platform shows increased cell viability, proliferation, chondrogenic and osteogenic differentiation of human bone-marrow-derived mesenchymal stem cells (hMSCs) <I>in situ</I>. The results show that the Alg/FDM hydrogel (CiB) matrix provides an excellent microenvironment for cell adhesion and can control the differentiation of hMSCs into specific lineages. Thus, these results suggest the potential applications of the Alg/FDM hydrogel matrix as a viable 3D culture system for tissue regeneration.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel biochemical and biophysical cues-integrated-biomaterial (CiB) platform is reported. </LI> <LI> CiB is composed of cells derived extracellular matrix (FDM) and alginate hydrogels. </LI> <LI> CiB mimics stem cells microenvironment, enhances cells viability and proliferation. </LI> <LI> CiB offers easy to fabricate 3D cell culture system. </LI> <LI> CiB induces specific chondrogenic and osteogenic differentiation of stem cells. </LI> </UL> </P>
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Park, Jimin,Du, Ping,Jeon, Jin‐,Kyung,Jang, Gun Hyuk,Hwang, Mintai Peter,Han, Hyung‐,Seop,Park, Kwideok,Lee, Kwan Hyi,Lee, Jee‐,Wook,Jeon, Hojeong,Kim, Yu‐,Chan,Park, Jong Woon WILEY‐VCH Verlag 2015 Angewandte Chemie Vol.127 No.49
<P><B>Abstract</B></P><P>Although the use of reactive oxygen species (ROS) has been extensively studied, current systems employ external stimuli such as light or electrical energy to produce ROS, which limits their practical usage. In this report, biocompatible metals were used to construct a novel electrochemical system that can spontaneously generate H<SUB>2</SUB>O<SUB>2</SUB> without any external light or voltage. The corrosion of Mg transfers electrons to Au‐decorated oxidized Ti in an energetically favorable process, and the spontaneous generation of H<SUB>2</SUB>O<SUB>2</SUB> in an oxygen reduction reaction was revealed to occur at titanium by combined spectroscopic and electrochemical analyses. The controlled release of H<SUB>2</SUB>O<SUB>2</SUB> noticeably enhanced in vitro angiogenesis even in the absence of growth factors. Finally, a new titanium implant prototype was developed by Mg incorporation, and its potential for promoting angiogenesis was demonstrated.</P>
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Preparation of New Bioactive Hybrid Bone Cements Containing Bis-GMA Derivatives as a Prepolymer
Park, Bang-Ju,Park, Kwideok,Ahn, Kwang-Duk,Chin, Yong-Ok,Han, Dong Keun Wiley - VCHVerlag GmbH & Co. KGaA 2006 Macromolecular Materials & Engineering Vol.291 No.6
<P>Summary: Novel organic-inorganic hybrid bioactive bone cements containing bisphenol-A-glycidyl methacrylate (Bis-GMA) derivatives and a bioactive inorganic filler were prepared for orthopedic applications. The Bis-GMA derivatives, such as 3MA and a 3MA mixture (3MA mix), were synthesized by blocking one or two of the hydroxyl groups of the Bis-GMA so as to use it as a prepolymer. Four organic prepolymers, such as Bis-GMA, 3MA, 3MA 50 and 3MA mix, and an inorganic filler, AW-GC, were used for the preparation of the bioactive bone cements and their characteristics were evaluated. As compared with the Bis-GMA control, the new bioactive bone cements containing the Bis-GMA derivatives exhibited appropriate curing times, low polymerization shrinkage, low water absorption and solubility as well as high mechanical properties. In particular, the bioactive bone cement containing 3MA mix and AW-GC had higher bending and compressive strengths than the Bis-GMA one.</P><P> <img src='wiley_img/14387492-2006-291-6-MAME200500408-gra001.gif' alt='wiley_img/14387492-2006-291-6-MAME200500408-gra001'> Graphic Curing time and polymerization shrinkage on various prepolymers of bioactive bone cements. </P>
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Park, Kwideok,Ju, Young Min,Son, Jun Sik,Ahn, Kwang-Duk,Han, Dong Keun Informa UK (TaylorFrancis) 2007 Journal of Biomaterials Science. Polymer Edition Vol.18 No.4
<P>Biodegradable polymers, such as poly(glycolic acid) (PGA), poly(L-lactic acid) (PLLA) and poly(lactic-co-glycolic acid) (PLGA), were dissolved individually in the proper solvents and then subjected to electrospinning process to make nanofibrous scaffolds. Their surfaces were then chemically modified using oxygen plasma treatment and in situ grafting of hydrophilic acrylic acid (AA). The fiber thickness, pore size and porosity were estimated to 200-800 nm, 2-30 microm and 94-96%, respectively, and these properties were insignificant in the PGA, PLLA and PLGA nanofibrous scaffolds. The ultimate tensile strength of PGA was about 2.5 MPa on average and that of PLGA and PLLA was less than 2 MPa. The elongation-at-break was 100-130% for the three nanofibrous scaffolds. When the surface properties of AA-grafted scaffolds were examined, higher ratios of oxygen to carbon, lower contact angles and the presence of carboxylic (-COOH) groups were identified. The properties were significantly different from those of the unmodified nanofibrous scaffolds. Fibroblasts once seeded on the scaffolds were spreading over large surface area on the AA-grafted surface as compared to the unmodified PGA, PLLA and PLGA nanofibrous scaffolds. Cultured for up to 6 days, the fibroblast proliferation was found to be much better on the surface-modified nanofibrous scaffolds. The present study showed that, with the use of plasma treatment and AA grafting, the hydrophilic functional groups could be successfully adapted on the surface of electrospun nanofibrous scaffolds. Those surface-modified scaffolds made significant improvement on cell attachment and proliferation in vitro.</P>
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Functional PLGA Scaffolds for Chondrogenesis of Bone-Marrow-Derived Mesenchymal Stem Cells
Park, Kwideok,Cho, Kyoung-Jin,Kim, Jae-Jin,Kim, Ik-Hwan,Han, Dong Keun WILEY-VCH Verlag 2009 Macromolecular bioscience Vol.9 No.3
<P>Two chondrogenic factors, Dex and TGF-β1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex-loaded PLGA scaffold was grafted with AA and heparin, the heparin-immobilized one was then reacted with TGF-β1, yielding a PLGA/Dex-TGF (PLGA/D/T) scaffold. The scaffolds were seeded with rabbit MSCs and cultured for 4 weeks. The results show that the scaffolds including chondrogenic factors strongly upregulated the expression of cartilage-specific genes and clearly displayed type-II collagen immunofluorescence. The functionalized PLGA scaffolds could provide an appropriate niche for chondrogenic differentiation of MSC without a constant medium supply of Dex and TGF-β1.</P><P> <img src='wiley_img/16165187-2009-9-3-MABI200800187-gra001.gif' alt='wiley_img/16165187-2009-9-3-MABI200800187-gra001'> </P>
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The Role of Biomechanics in Tissue Engineering
박귀덕(Kwideok Park) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.11
Tissue engineering is an interdisciplinary field that utilizes the principles of engineering and life sciences toward the creation of biological substitutes. Traditionally, major components of tissue engineering are cells, scaffolds, growth factors and recently biomechanical aspects have been given much attention. A large number of studies have reported that mechanical signals are of particular interest in either encouraging or inhibiting cellular responses. In tissue engineering, cell adhesion is a very important step, because quality of adhesion may determine a cell fate in the future. Elasticity of cell-adhesive substrate is found critical in regulating stem cell differentiation. Cells exert different contractile forces for cell migration, depending on substrate mechanics. Though tissue engineering is very interactive with diverse expertise, for a breakthrough, principles of biomechanics in tissue and cell level needs to be fully understood.
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Preparation and investigation of hydrolyzed polyacrylonitrile as a preliminary biomedical hydrogel
Ji Hoon Park,Guo Zhe Tai,Bo Keun Lee,Seung Hun Park,Ja Yong Jang,Jung Soo Lee,Jae Ho Kim,Kwideok Park,장주웅,김문석 한국생체재료학회 2015 생체재료학회지 Vol.19 No.4
Background: Hydrolyzed polyacrylonitrile (HPAN) has attracted much attention as a hydrogel for a broad range of biomedical applications. Therefore, in this study, we prepared HPAN derivatives with controllable compositions by the radical polymerization of acrylonitrile (AN), methacrylic acid (MAA) and N-isopropylacrylamide (NIPAM) monomers. Results: The prepared poly(AN-co-MAA-co-NIPAM) copolymers had different ratios of AN, MAA, and NIPAM and molecular weights ranging from 2000 to 50,000. The copolymers were prepared as films to examine their properties. The prepared copolymer films showed different solubilities, contact angles, and swelling ratios. The properties of the copolymer films were affected by the hydrophobic PAN segments and the hydrophilic PMAA or PNIPAM segments. Conclusion: Thus, we conclude that introducing PMAA and PNIPAM segments with different ratios and lengths into PAN segments could represent a method of controlling the hydrogel properties of copolymers.
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