한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성

이승환,김승창,조수현,최봉환,Aditi Sharma,임다정,당창권,장선식,김재환,고문석,양보석,강희설 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.

濟州島 俗談의 表現

高在奐 濟州敎育大學校 1986 論文集 Vol.16 No.-

The expression of Korean proverbs were not different from the forms, but the former was treated as a morpheme concerned with the latter.In this study I treated the expression and form of proverbs seperately from each other, and encouraged that the intention of proverbs would be increased by literary expressions as well as forms. Especially, I made a survey of the rhetoric, metrical scheme and comicality of 550 Cheju-do proverbs. The result of survey is as follows: 1. RHETORIC ; all of the proverbs could be accounted for by means of 10 rhetorics. The proverbs were written in the order of epigram, indirect suggestion, metaphor, contrast, parallelism, hyperbole, simile exhortation, paradox and gradation. 2. METRICAL SCHEME ; as the expression of proverbs is similar to that of poetry, Korean songs and lyrics can now accommodate only absolute pitchs and musical charactders, not rhyme appointed as disappropriate to Korean peotry. 3-4 syllable meter, 4-4 syllable meter, and 7-5 syllable meter, which is considered as traditional rhythem of Korean poetry, cover most of the proverbs in distribution. It means that there exists a close relationship between proverbs and poetry. Musical characters are various from 1-notation to 17-notation, and the usage rate appeared in the order of 4-notation, 2-notation, 3-notation and etc. From this, it is estimated that the old poetry had changed into 4-notation while they came down to Korea dynasty. 3. COMICALITY ; the application of a sarcasm, humor, wit, irony called subjective comicality to proverbs makes persons realize that proverbs are a aliterary experssion. Even though the sarcasm is less exciting, it enlighten perosns on their social position and wisdom. Humor criticizes comically discordance between persons' words and actions on the basis of crude and local temper. Irony has a phylosophy, the arts of life through abandonment, resistance, self-conquest, self-scorn and self-complaint. Wit acts as the gateway to represent human affairs, because normal mentality is experssed through abnoram mentality and the reverse is applicable. As shown above, the expression of proverbs is similar to that of literary works. Consequently, even if it is fragmentary, this leads to a renewed appreciation of the literary character of proverbs.

정신분열병환자의 회화에 나타난 예후적 특징에 관한 일 연구

고대관,김재환 大韓神經精神醫學會 1990 신경정신의학 Vol.29 No.4

This study was attempted to examine the validity of the characteristics which had been suggested as the prognostic signs in the human figure drawings of the schizophrenics. Subjects were 63 schizophrenics and 44 non-psychotic patients. As a result, there were significant differences between two groups in reference to following parameters ; media(p<.05), global expressiveness(p<.01), drawn body area(p<.05), number of color used(p<.01), Arieti effects-spatial alteration(p<.05), infantilization(p<.058), detail(p<.01), and emphasized body area(p<.05). It has been thought that seven of these pronostic signs could provided the basic data for the future study of prognostic signs in drawings of the schizophrenics.

濟州産 한라봉 柑橘의 방사선 조사에 의한 貯藏性

고영환,김재하 제주대학교 방사능이용연구소 2001 연구보고 Vol.15 No.-

방사선 照射가 한라봉 감귤의 저장에 미치는 영향을 조사하였다. 한라봉 신선과일에 Cobalt 60으로 감마선을 0 kGy, 1 kGy, 2 kGy 그리고 3kGy를 각각 조사하여 실온 및 저온(6℃)에 저장하였다. 저장중의 부패율, 중량 변화, pH, 가용성고형분을 측정하였고, 부패와 관련된 미생물을 분리 동정하였다. 저온저장이 실온저장보다 부패방지와 갈변억제에 보다 더 효과적었다. 방사선을 조사하지 아니한 처리구나 1kGy 조사한 처리구에 비해서, 2 kGy이상 γ-방사선을 조사한 처리구는 부패와 갈변 정도가 심하였다. 저장 중에 감귤의 부패와 관련하여 나타나는 미생물은 대부분이 Penicillium 속이었다. 저온 저장했을 때가 실온 저장했을때보다 중량감소율이 더 낮았다. 또한 γ선을 2kGy 이상 조사했을 경우에는 상대적으로 중량감소가 크게 나타났다. 저장 중에 감귤의 pH와 당도는 저장기간이 경과함에 따라서 전반적으로 증가하는 경향이었다. 이러한 경향은 실온저장과 저온저장 그리고 방사선 조사구와 비조사구간에는 별다른 차이가 없었다. 전반적으로 γ 방사선을 2kGy 이상 조사하면, 감귤의 저장에 부정적인 효과가 나타났으며, 1 kGy 조사한 처리구와 방사선을 조사하지 아니한 대조구 간에는 유의적인 차이가 인정되지 않았다. 따라서, 감귤의 저장에는 방사선 조사선량을 1 kGy이하로 하는 것이 바람직하다. Effects of γ-ray irradiation on storage of citrus orange Halabong were investigated. γ-Ray was irradiated on fresh fruits of Halabong on the levels of 0 kGy, 1 kGy and 3 kGy using cobalt 60 as a radiation source. Irradiated fruits were stored at room or cold(6℃) temperatures for storage. During storage, changes in decay, weight , pH and soluble solids were monitored timewisely, and molds related with fruit decay were isolated and purified. Storage at cold temperature was more effective against decay and browning. The pretreated fruits with higher dose radiation than 2 kGy showed severe decay and browning. Most of the molds associated with fruit decay were Penicillium species. Weight loss during cold storage was less than that during room temperature storage. And irradiation of fruits with higher dose radiation than 2 kGy gave higher weight loss. Soluble solid content and pH of juice increased on the whole as the storage time went by. This tendency was all similar in every cases of fruit storage whether stored at room temperature or cold temperature regardless of γ-ray irradiation. In conclusion, higher dose radiation than 2 kGy resulted in negative effective on fruit storage, and no significant difference on the whole was observed between non-irradiated fruits and 1 kGy-irradiated fruits. It is highly recommended that citrus orange Halabong should be irradiated with lower dose of radiation than 1 kGy when necessary.

濟州島俗談의 主題

高在奐 제주대학교 탐라문화연구소 1988 탐라문화 Vol.0 No.7

고등학교 축구 지도자의 리더십 행동유형과 선수 만족도의 관계

송광환,정재은,고의석,박윤식,육동원 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

The purpose of this study was to examine behavioral types of efficient soccer coaches by identifying relationship between athletic satisfaction and leadership behavior types of high school soccer coaches. who have an impact on soccer players. The study also took a closer look at the types of leadership behavior shown by soccer players depending on their school level, position and status-whether or not a key player or a bench warmer. A total of 287 high school soccer players were subjected to the study and the Leadership Scale for Sport (LSS) and the Satisfaction Scale for Soccer Players were used to obtain the study results. In terms of leadership behavioral type by school level. good schools were found to hold higher levels of perception on instruction behavior. autocratic behavior. democratic behavior and social support behavior than the relatively poor schools. No significant difference was found in terms of leadership behavior type by position. However, the analysis on the leadership behavior type by players status showed that the key players had a higher perception on instruction and training behaviors than the bench warmers. According to the athletic satisfaction by school level, no significant difference was found in sub-variables. The same was true for both athletic satisfaction by position and athletic satisfaction by players status. In terms of correlation between the leadership behavior type and athletic satisfaction, training and instruction behavior. democratic behavior, social support behavior and positive feedback behavior were found to have a significant correlation with the sub-variables of athletic satisfaction, which are, sport performance satisfaction and psychological satisfaction. Moreover, the leadership behavior type, training and instruction behavior and autocratic behavior were identified as variables that have a significant impact on sport performance satisfaction and psychological satisfaction, which are sub-factors of athletic satisfaction.

보신 관광에서 감염된 수입성 Sparganosis 1예 : Intake of Thailand Snake during Trip

서환조,박명재,고경식,백영한,조유정,양현종,류경남,정형근,전정열 대한감염학회 1992 감염 Vol.24 No.1

Human sparganosois was reported more than 100 cases in Korean population. However no case reported yet in Korea as a patient was infected by sparganum from other geographical area, such as Thailand. The case we are reported here is a 38 year-old Korean male who had been suffered from mass being a small, round and soft consistance in upper portion of his right thigh. The patients stated that he had an opportunity to eat living snake in Bangkok, Thailand during trip. As therapy praziquantel (75㎎/㎏/day) was given p.o. for 14 days, but in vain to cure. Following medical treatment, surgical exicision was performed to remove a alive, soft, whitish-milky, mobile and elongated sparganum. Serlogical test by micro-ELISA for sparganum-specific antibody revealed a high serum antibody level of absorbance 0.67 (positive criterion: absorbance 0.22).

低鹽濃度의 자리醱酵食品의 加工에 關한 硏究

河璡桓,高榮煥,金洙賢,姜永周,金在河,宋大鎭 濟州大學校工科大學附屬産業技術硏究所 1990 尖端技術硏究所論文集 Vol.1 No.-

제주도의 전통수산식품인 자리젓에 대한 식품학적 기초자료를 얻고 또한 저염화 방안을 찾아 향토관광식품으로 개발하고자 실험한 결과는 다음과 같다. 자리젓의 최적숙성기는 첨가식염농도가 25%, 12.5% 및 10%의 것은 각각 60일 전후였으며 첨가식염농도가 7.5% 및 5%인 것은 약 45일 경우에 가장 맛이 좋았다. 원료 자리돔에서는 핵산관련물질 중 IMP가 17.7 μmole/g으로 가장 많았으나 30일 이상 숙성시킨 경우 전시료에 걸쳐 ATP와 ADP는 검출되지 않았으며 hypoxanthine이 핵산관련물질의 대부분을 차지하였다. 원료 자리돔에서는 17종이, 자리젓에서는 16종의 유리아미노산이 검출, 동정되었다. 숙성60일째의 자리젓에서 함량이 많은 아미노산은 lysine, alanine, glutamic acid, glycine 및 leucine으로 이들이 총유리아미노산의 약 60%를 차지하였다. TMAO는 숙성기일의 지남에 따라 서서히 감소하여 75일째에는 거의 소실되었고 TMA는 45일 까지 급격히 증가하다가 이후 급격히 감소하였다. Pediococcus acidilactici를 starter 로 106cells/㎖접종한 시료에서는 숙성 15일만에 세균수는 최대치에 도달하였으며 45일 이후에는 급격히 감소하였다. 그러나 Pediococcus halophilus 를 ?? 접종한 경우 숙성기간중 유산균 수에는 큰 변화가 없었다. 저염자리젓의 지방산 조성은 포화산이 31.9%내외, 모노엔산이 13.2% 내외 그리고 폴리엔산이 54.9% 내외였다. 폴리엔산 중 EPA는 1.9%정도였으나 DHA가 23.0% 내외로 높은 분포를 보였다. 자리젓의 저염화를 위하여 Pediococcus halophilus를 starter로 첨가하고 식염 10-12.5% KCl 4-5%, 포도당을 2% 수준으로 첨가하면 재래식 젓갈에 손색이 없는 저염자리젓의 제조가 가능할 것으로 판단되었다. This study was carried out to obtain the basic data on salted and fermented damsel fish(Jari-Jeot). One of the important traditional marine foods in Cheju-Do, and also to seek salt lowering method for the development of a new local tourist food. The results are as follows: The best organoleptic results were obtained after 60 days' fermentation for 25.0, 12.5 and 10% of salt-added group, and around 45 days for 7.5 and 5% of salt-added group. In raw fish ingredient IMP was abundant which marked 17.7 μmole/g while in fermented fish hypoxanthine was predominant but ATP and ADP were not detected after 30 days' fermentation. Sixteen kinds of free amino acids were detected and identified in Jari-Jeot instead of 17 kinds of those in raw sample. The abundant amino acids in Jari-Jeot after 60 days' fermentation were lysine, alanine, glutamic acid, glycine and leucine, and those were consisted of approximately 60% of the total free amino acids. During fermentation the content of TMAO was decreased gradually and no TMAO was detected after 75 days' fermentation while that of TMA was increased during fermentation up to 45 days' and decreased afterwards. The fatty acid of low-salt Jari-Jeot was composed of 31.9% of saturated acids, 13.2% of monoenoic acids and 54.9% of of polyenoic acids. While the content of EPA in polyenoic acids was 1.9%, that of DHA was 23.0%. In order to lower salt concentaration of fermented damsel fish, addition of Pediococcus halophilus culture as a starter together with 10-12.5% of salt, 4-5% of KCl, and 2% of glucose gave as good quality as traditionally made Jari-Jeot.

Mild Cognitive Impairment 노인군의 점화효과 특성

박재우,김도관,고선규,고효정,김성환,김재경,서민영,이인수,김지혜 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.3

연구목적 : 이 연구에서는 K-MMSE에서 25점 이하를 받은 50대 이상 노인들을 MCI(Mild Cognitive Impairment) 군으로 보고 이들의 인지 기능 특성을 점화 효과를 중심으로 살펴보고자 하였다. 먼저, 점화 효과가 MCI 노인군에서 있어서도 유지되고 있는지를 알아보고, 인지 기능의 손상 정도에 따라 점화 효과가 유의미하게 변화하는 지를 살펴보고자 하였다. 방 법 : 본 연구에서는 K-NAME 점수 25점 이하의 노인 96명으 대상으로 ADAS-Cog와 일종의 간접 기억 검사 도구인 지각적 파악 과제를 제작하여 점화 효과를 측정하여 인지 기능의 퇴행이 시작된 고연령군이 나타내는 암묵 기억 능력 특성을 알아보았다. 점화효과는 컴퓨터 상에 사전 노출된 자극과 그렇지 않은 자극을 순간 노출시켜 미리 노출된 자극에 대해 반응 시간이 줄어들고 정확 반응이 증가하는 정도로써 측정하였다. 이 때 지능이나 연령, 학력 수준과 같은 변인이 미치는 영향을 통제하기 위해 한국판 웩슬러 지능 검사의 <어휘>, <토막> 소검사를 실시하였고, 인구통계학적 변인에 관한 면접을 실시하였다. 통계적 분석 과정에서는 먼저, 연구에서 측정된 점화효과의 특성을 알아보기 점화 효과 측정치의 기술 통계치를 구하였으며 다른 측정 변인들과의 상관 분석을 실시하였다. 마지막으로 측정된 점화효과가 인지 기능의 퇴행 수준에 비례하는 지의 여부를 파악하기 위해 ADAS-Cog 및 나이, 교육 연한 및 지능 수준을 독립변인으로 회귀분석을 실시하였다. 결 과 : 먼저 기술 통계 분석 결과에 의하면 본 연구에 참여한 피보험자들의 경우 점화효과에 의해 미리 노출된 자극을 평균 3.6개 더 정확히 파악하였으며 평균 59ms 더 빠르게 반응한 것으로 나타나 이들 집단에서도 점화효과가 유지되고 있음을 알 수 있었다. 다음으로 변인들 간의 상관 분석 결과에 따르면, 정확 파악 반응 점수에 따른 점화효과는 전반적인 인지 기능을 평가하는 K-MMSE 및 ADAS-Cog와 정적인 상관이 있는 것으로 나타났으며 반응 속도로 측정한 점화효과의 경우에는 나이와는 정적인 상관이, KWIS의 <어휘> 소검사 점수와는 부적인 상관이 있는 것으로 나타났다. 또한, 본 연구에서 사용된 점화 효과 측정치를 종속 변인으로 ADAS-Cog와 KWIS의 어휘 및 코막 소검사로 측정한 지능 수준, 나이와 교육 연한과 같은 인구 통계학적 변인을 독립 변인으로 회귀 분석을 실시한 결과에 따르면 정화간응 점수로 측정한 점화 효과의 경우 상관 분석 결과에서와 같이 ADAS-Cog 변인만이 유의미한 효과를 갖는 것으로 나타났으며 반응 속도로 측정한 점화 효과의 경우 나이의 효과만 유의미한 것으로 나타났다. 결 론 : 이러한 결과는 경미한 인지 기능의 손상이 진행되고 있는 고연령군의 경우에는 외현 기억 능력은 손상이 시작되지만 점화효과로 측정된 암묵 기억이 전반적으로는 유지되다가 인지 기능의 퇴행이 진행됨에 따라 노출된 자극을 더 정확히 파악하는 능력이 저하되고 점화 효과에 의해 인출 속도가 빨라지는 현상이 연령이 증가함에 따라 함께 증가하는 것으로 나타남을 말해 준다. Objectives : In this study, the cognitive characteristics, especially priming effect, of above 50 years old age group who had less than 25 points in K-MMSE were investigated. Methods : In this study, an implicit memory test measuring priming effect was made and administered ti old age people who had less than 25 points in k-MMSe in screening session. To control the effect of age, educational level and intelligence, demograohic variables were measured, and the subjects of KWIS, vocabulary and block design were administered. Descriptive statistical analysis of the two priming measures and correlational analysis between variables were done. To test the effect of cognitive functioning on priming effect multiple regression analysis was done. Results : Correlational analysis revealed priming score obtained from correct identification response was positively correlated with K-MMSE and ADAS-Cog. And priming score obtained from mean reaction time was positively correlated with age and negatively correlated with vocabulary substest of KWIS. The regression analysis results indicated from correct identification response, whereas age has significant effect on priming score obtained from mean reaction time. Conclusions : The results revealed that as cognitive deterioration progresses priming effect which identify primed stimulus correctly diminish, and as one grow older within age 50 to 70, priming effect which identifies primed stimulus quickly increase.

연령이 다른 Bald/Caj 생쥐의 피부창상치유중 섬유모세포의 동원

유영현,김동환,고재승,한성수,한성수,한성수 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.4