Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

The Variation of Winter Buds among 10 Selected Populations of Kalopanax septemlobus Koidz. in Korea

Kim,Sea-Hyun,Ahn,Young-sang,Jung,Hyun-Kwon,Jang,Yong-Seok,Park,Hyung-Soon 한국자원식물학회 2002 Plant Resources Vol.5 No.3

The objective of this study was to understand the conservation of gene resources and provide information for mass selection of winter bud characters among the selected populations of Kalopanax septemlobus Koidz using analysis of variance(ANOVA) tests. The obtained results are shown below; 1. Ten populations of K. septemlobus were selected for the study of the variation of winter bud characters in Korea. The results of the analysis of variance(ANOVA) tests shows that there were statistically significant differences in all of the winter bud characters among those populations. 2. Correlation analysis shows that width between Height and DBH(Diameter at breast height) characters have negative relationship with all of the characters, as ABL(Apical branch length), ABW(Apical branch width), AWBL(Apical branch winter bud length), AWBW(Apical branch winter bud width), ABT(Apical branch No. of thorns), ABLB(Apical branch No. of lateral bud) and LBL(Lateral branch length), LBW(Lateral branch width), LBT(Lateral branch No. of thorns), LBLB(Lateral branch No. of lateral bud). 3. The result of principal component analysis(PCA) for winter buds showed that the first principal components(PC s) to the fourth principal component explains about 78% of the total variation. The first principal component(PC) was correlated with AWBW, LWBW, and LBL and the ratio of ABL/ABW and LBL/LBW out of 16 winter bud characters. The second principal component correlated with ABL, ABW, ABLB, LWBL(Lateral branch winter bud length), and LBW and the ratio of AWBL/AWBW. The third principal component correlated with ABL, ABW, LWBL, LBL, and the ratio of LBL/LBW. The fourth principal component correlated with LBL and the ratio of LWBL/LWBW(Lateral branch winter bud width), LBL/LBW. Therefore, these characters were important to analysis of the variation for winter bud characters among selected populations of K. septemlobus in Korea. 4. Cluster analysis using the average linkage method based on 10 selected populations for the 16 winter bud characters of K. septemlobus in Korea showed a clustering into two groups by level of distance 1.1(Fig. 3). As can be seen in Fig. 3, Group I consisted of three areas(Mt. Sori, Mt. Balwang and Mt. Worak) and Group Ⅱ contisted of seven areas(Suwon, Mt. Chuwang, Mt. Kyeryong, Mt. Kaji, Mt. Jiri, Muan, and Mt. Halla). The result of cluster analysis for winter bud characters corresponded well with principal component analysis, as is shown in Fig. 2.

공급사슬에서의 리스크 대응이 공급사슬통합과 리스크 관리 성숙도, 기업경영성과에 미치는 영향

박찬권(Park, Chan-Kwon),박성민(Park, Sung-Min),김채복(Kim, Chae-Bogk) 한국경영교육학회 2021 경영교육연구 Vol.36 No.2

[연구목적] 본 연구는 공급사슬에서 발생할 수 있는 리스크를 규명하고, 리스크에 대응하기 위한 과정에서 공급사슬통합의 필요성과 함께 리스크 관리 성숙도 제고가 기업경영성과 역시 제고할 수 있다는 것을 규명하는 것이다. 이를 위해 리스크 대응과 공급사슬통합, 리스크 관리 성숙도, 기업경영성과 간의 관계구조를 분석하고 시사점을 제공하는 것이다. [연구방법] 본 연구는 공급사슬에서 발생할 수 있는 리스크 유형, 공급사슬통합, 리스크 관리 성숙도, 기업경영성과에 대한 기존 선행연구들과 논의를 충분히 검토하였고 최종적으로 6개의 가설을 도출하였다. 연구목표 달성을 위해 국내 제조업체들을 대상으로 124개의 설문 자료를 수집하였다. 수집된 자료에 대해 신뢰성과 타당성을 검토하였고, Smart PLS 2.0을 활용하여 경로분석 방식으로 연구가설을 검증하였다. [연구결과] 내부리스크대응, 외부리스크대응, 공급사슬리스크대응은 공급사슬통합에 유의한 정(+)의 영향을 미치며, 내부리스크대응과 공급사슬리스크대응은 리스크관리성숙도에 정(+)의 영향을 미친다. 공급사슬통합과 리스크 관리 성숙도는 기업경영성과에 정(+)의 영향을 미친다. 또한 공급사슬통합은 외부리스크대응 및 공급사슬리스크대응과 기업경영성과 사이에서 매개변수의 역할을 하는 것으로 검정되었다. [연구의 시사점] 공급사슬에서의 리스크 대응, 공급사슬통합, 리스크 관리 성숙도, 기업경영성과 간의 전체적인 관계구조에 대하여 연구를 진행하였으며, 우리나라의 기업들에게 리스크 대응과 관련한 이론적, 실무적 시사점을 제공하였다. [Purpose] This study is to identify risks that may occur in the supply chain, and to identify that supply chain integration is necessary in the process of responding to the risks, and that corporate management performance can also be improved by enhancing risk management maturity. The purpose of this study was to analyze the relationship structure between risk response, supply chain integration, risk management maturity, and corporate management performance and to provide implications. [Methodology] This study has sufficiently reviewed the previous studies and discussions on the types of risks that can occur in the supply chain, supply chain integration, risk management maturity, and business management performance, and finally derived six hypotheses. For the study, 124 data were collected from domestic manufacturers. The reliability and validity of the collected data were reviewed, and the research hypothesis was verified through a path analysis method using Smart PLS 2.0. [Findings] Internal risk response, external risk response, and supplier chain integration have a significant positive (+) effect on supply chain integration, while internal risk response and supplier chain risk response have a positive (+) effect on risk management maturity. Supply chain integration and risk management maturity have a positive (+) effect on corporate management performance. In addition, it was verified that supply chain integration plays a role as a parameter between external risk response and supplier chain risk response and corporate management performance. [Implications] A study was conducted on the overall relationship structure between risk response in the supply chain, supply chain integration, risk management maturity, and corporate management performance, and provided theoretical and practical implications for risk response to Korean companies.

Fluoxetine Treatment in Poststroke Depression, Emotional Incontinence, and Anger Proneness : A Double-Blind, Placebo-Controlled Study

Choi-Kwon, Smi,Han, Sung W.,Kwon, Sun U.,Kang, Dong-Wha,Choi, Ji M.,Kim, Jong S. Ovid Technologies Wolters Kluwer -American Heart A 2006 Stroke Vol.37 No.1

<P>BACKGROUND AND PURPOSE: The efficacy and safety of the selective serotonin reuptake inhibitor fluoxetine have rarely been studied in the treatment of poststroke emotional disturbances. METHODS: Stroke patients (152) who had poststroke depression (PSD), emotional incontinence (PSEI), or anger proneness (PSAP) were studied. PSD was evaluated by Beck Depression Inventory and Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, PSEI by Kim's criteria, and PSAP was assessed by Spielberger Trait Anger Scale. Subjects were randomly given either fluoxetine 20 mg/day (n=76) or placebo (n=76) for 3 months. Follow-up evaluations were done 1, 3, and 6 months after the beginning of the treatment. The primary outcome measurement was the scores of emotional disturbances at each follow-up assessment. The secondary outcome measurements were the percentage changes of the scores and the subjective responses of the patients. RESULTS: Although patients in the fluoxetine group more often dropped out because of adverse effects, fluoxetine administration was generally safe. Fluoxetine significantly improved PSEI and PSAP, whereas no definitive improvement of PSD was found. Improvement of PSAP was noted even at 3 months after the discontinuation of the treatment. CONCLUSIONS: Fluoxetine is efficacious in the treatment of PSEI and PSAP. Its effect on PSD is not solidly confirmed.</P>

Long-Term Changes in Post-Stroke Depression, Emotional Incontinence, and Anger

Boseong Kwon,Eun-Jae Lee,박성호,Ji Sung Lee,Min Hwan Lee,Daeun Jeong,Dongwhane Lee,Hyuk Sung Kwon,Dae-Il Chang,Jong-Ho Park,Jae-Kwan Cha,허지회,Sung-Il Sohn,Dong-Eog Kim,Smi Choi-Kwon,Jong S. Kim 대한뇌졸중학회 2021 Journal of stroke Vol.23 No.2

Background and Purpose Long-term changes in post-stroke depression (PSD), post-stroke emotional incontinence (PSEI), and post-stroke anger (PSA) have rarely been studied. Methods This is a sub-study of EMOTION, a randomized, placebo-controlled trial, that examined the efficacy of escitalopram on PSD, PSEI, and PSA in patients with stroke. We interviewed patients at the long-term period (LTP) using predefined questionnaires: Montgomery-Åsberg depression rating scale (MADRS) for PSD, modified Kim’s criteria for PSEI, and Spielberger trait anger scale for PSA. Additionally, the ENRICHD Social Support Instrument (ESSI) for the social support state and the modified Rankin Scale (mRS) were measured. We investigated the changes in and factors behind PSD, PSEI, and PSA at LTP. Results A total of 222 patients were included, and the median follow-up duration was 59.5 months (interquartile range, 50 to 70). Compared to the data at 6 months post-stroke, the prevalence of PSEI (11.7% at 6 months, 6.3% at LTP; P=0.05) and mean anger score (21.62, 16.24; P<0.01) decreased, while the prevalence of PSD (35.6%, 44.6%; P=0.03) and mean MADRS (6.16, 8.67; P<0.01) increased at LTP. ESSI was associated with PSD and PSA, but not with PSEI. The effect of the baseline National Institutes of Health Stroke Scale score on PSD decreased over time. The effect of low social support on PSD was greater than that of mRS at LTP. Conclusions The prevalence and degree of PSD significantly increased, while those of PSEI and PSA decreased at LTP. PSD in this stage appeared to be more closely associated with a lack of social support than patients' physical disabilities.

Identification of a novel peptide that selectively binds to Kidney Injury Molecule-1 (KIM-1) by phage displayed peptide library

Tae-Jun Kwon,Joon-Suk Park,Sang-Hyun Kim,Enamul Haque,Byung-Heon Lee,Sang Eun Kim,Choong-Yong Kim,Sang Kyoon Kim 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.2

Single-Port Robotic Cholecystectomy: Early Experience from 8 Cases

( Hyung Jun Kwon ),( Horyon Kong ),( Sang Geol Kim ),( Yun Jin Hwang ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Single-port laparoscopic cholecystectomy (SPLC) is a technical concept to reduced pain and improve cosmetic results when compared to multi-port cholecystectomy. However, SPLC is associated with technical limitation due to the enhanced complexity of the approach and limited number of specialized instruments or platforms. On the other hand, using a robotic platform may overcome these problems and enable more precise surgical actions by increasing freedom of movement and by restoring intuitive instrument control. In this presentation, we report the early clinical experience of our first 8 sing-port robotic cholecystectomy (SPRC) cases. Methods: Between November 2016 and February 2017, eight patients underwent SPRC with the da Vinci Xi robot and single-site instrumentation. We retrospectively reviewed clinical data on those patients. Results: All of 8 patients had completion of SPRC. Seven patients were female and one was male. Average patient age was 43.3±11.8 years and BMI was 22.4±1.4 kg/m2. Three patients (37.5%) were diagnosed with chronic calculous cholecystitis. Three patients (37.5%) underwent operation for polypoid lesions of the gallbladder. One patient (12.5%) was diagnosed with acute calculous cholecystitis. The mean operation time (skin-to-skin) was 83.9±30.7 min, docking time was 13.25±8.1 min, and console time was 42.1±26.4 min. The intraoperative blood loss was negligible. The mean Visual Analog Pain Scale score 6hr after the surgery was 2.9±0.4. The mean length of hospital stay average postoperative hospital stay was 2.3±1.0 day. There were no intraoperative complication and one patient developed seroma on port site. Conclusions: Robotic single-port cholecystectomy appears feasible and safe in our early experience.

The level of urinary aflatoxin M1 in Korean adults

Yong-Dae Kim1, Hyojin Kwon, Sun-In Moon, Sang-Yong Eom, Jung-Duk Park, Byung-Sun Choi, Seok-Joon Sohn, Young-Seoub Hong, Ho Kim, Ho-Jang Kwon, Ji-Ae Lim, Hae-Jung Yoon, Gwang-Jin Kim, Heon Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Competitive ELISA methods were used to measure the level of aflatoxin M1 (AFM1) from urine in 1008 Korean adults. Subjects were selected by random sampling in all areas of Korea, except Cheju-do. The recovery rate of AFM1 using this method was 105% (73-124%). The geometric mean of urinary AFM1 in all subjects was 3.43 pg/mL (3.67 ng/g creatinine). The level of AFM1 in males was statistically higher, compared with female subjects. However, the levels of AFM1 did not differ according to age. Subjects in Chungbuk-do showed the highest urinary AFM1 concentration, whereas subjects in Kyeongnam-do showed the lowest concentration. Assuming an excretion rate of 5%, this AFM1 excretion corresponds to approximately 0.1 microgram/day in Korean adults.

Kidney Toxicity Induced by 13 Weeks Exposure to the Fruiting Body of Paecilomyces sinclairii in Rats

Jeong, Mi-Hye,Kim, Young-Won,Min, Jeong-Ran,Kwon, Min,Han, Beom-Suk,Kim, Jeong-Gyu,Jeong, Sang-Hee Korean Society of ToxicologyKorea Environmental Mu 2012 Toxicological Research Vol.28 No.3

Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (${\beta}2m$), glutathione S-transferase alpha (GST-${\alpha}$), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegaly and tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats with histopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.

제조업체 근로자의 직업관련성 근골격계질환의 유병율에 관한 연구

김성미,조영하,김용권,Kim, Sung-Mi,Jo, Young-Ha,Kim, Yong-Kwon 대한물리치료과학회 2004 대한물리치료과학회지 Vol.11 No.3

The purpose of this study was carried out to rind out the prevalence of occupational musculoskeletal disorders among workers in manufacturing industries, so that the result could provide basic data necessary to prevent musculoskeletal disorders. Information on general characteristics, occupational characteristics, and musculoskeletal symptoms were obtained by a self-administered questionnaire between October and November in 2002, from 345 workers in Kim-hae and Ulsan, Kyung-nam province. The data were analyzed for chi-square test by using SPSS NVIN 10.0 program. The results are as follows: According to the self-reports, among musculoskeletal symptoms complain on shoulders are topping for 50.4%, low back is followed for 40.6%, leg/foot 35.7%, neck 34.5%, wrist/hand/finger 30.1%, and arm 24.3%. According to occupational characteristics prevalence by anatomical site, about neck pains 119 subject, occupational satisfaction is 62,6% in moderate group, work shift is 71.8% in no work shift group, each significant high. Symptom complain rate of 104 patients who complained on wrist, hand and finger pain is significantly related that 36.5% below 5 years and 36.5% above 15 years have been worked group. And each of them are significantly related 77.9% in labor workers group, 70% in no work shift group, 54.8% in frequent transfer group. Among 140 subjects who have back pain, that is significationtly reported on 37.1% below 5 years and 37.9% above 15 years and 60.0% moderate satisfied occupation group have been worked group. Therefore, some efforts should be proceeded such as improvement of working condition, flexibility for changing work, more pleasant and better working environment, and etc.