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김동수,박지현,류병호,양승택,문윤희,김희숙 慶星大學校 1993 論文集 Vol.14 No.4
Attempts were made to investigate the levels of chemical components and detoxification of the salted puffer overies, Fugu xanthopterus, "Ggachibog" and Fugu rubripes rubripes, "Chambog", processing of solid salt mixtures containing sodium bicarbonate, during storage period. Fresh ovaries of the puffers were divided into three portions, sprinkled with a solid salt mixture containing 0,1 or 2% NaHCO₃, and were stored at room temperature for 12 weeks. The samples were examined at each step and assayed for pH, VBN, amino-N, toxicity and thin- layer chromatogram pattern. The results were summarized as follows: The values of pH were appeared to be different with the added amount of siodium bicarbonate and the values of pH were over 7.0 in a salt mixture containing 2% NaHCO₃. The change of VBN value was also revealed about 85mg/100g in A division and 90-100mg/100g in both B and C, respectively, in the salted ovary of puffer "Ggachibog" from 8 weeks. In case of salted product from the "Chamboh" ovary, it had slightly lower value than that of "Ggachibog" ovary. Also, the value of amino-N was increased until 4-6 weeks and was slowly down after the weeks in all samples. All salted products were found to lose most of the toxicity during storage period when the salt containing NaHcO₃was used for sprinkling. In the case of raw ovaries being weak toxicity below 5.0 MU/g or 24.1 MU/g, the toxicity of salted product was dropped near the level to be edible. Therefore there was significant detoxification of the puffer ovaries by the salt mixture containing sodium bicarbonate. Finally, the toxins isolated from each raw puffer ovary were detected to be TTX-related components by thin-layer chromatogrphy.
자궁경부 세포진 검사에서 기존의 방법과 ThinPrep 법의 비교 연구
김희숙,김대곤,박종택,심재욱,문명진,박종숙,장희숙,정환욱,이기헌,윤경호,박인서 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.8
Objectives : The purpose of this study was to evaluate the clinical usefulness of the automated fluid-based thin layer preparation (ThinPrep Pap test). Specimen adequacy and diagnostic detection rates for ThinPrep Pap Test(TP) were compared with conventional Pap smears(CP). Methods : A total of 504 women were included in this study. The cervical smears were performed by three different physicians. A plastic Ayre's spatula and a Cytobrush were used for the collection of ecto- and endo-cervical cells. Split-sampling study was done like that a CP slide was made first and then the sampling devices were immediately rinsed into a vial containing preservative fluid (PreservCyt; Cytyc corporation) to release the cells on sampling devices, from which a TP slide was made by ThinPrep 2000 Processor automated slide preparation system (Cytyc corporation). All TP slides and CP slides were screened and interpretated by two cytotechnologists and one pathologist, respectively. The Bethesda System (TBS) classification was applied. Results : The TP presented a cleaner background, uniform cellularity, and enhanced nuclear details than the CP. Although there was no statistical significance, the TP showed increased number of $quot;unsatisfactory$quot; and $quot;satisfactory but limited by (SBLB)$quot; specimens than CP, mostly due to hypocellularity and absence of transformation zone components. Despite the increased number of inadequate specimens, the diagnostic detection rates of the TP were slightly greater but not statistically significantly different than the CP, irrespective of the disease categories. Exact diagnostic agreement between TP and CP was 98.39%. The TP yielded a higher proportion of low and high grade squamous intraepithelial lesions (LSILs and HSILs), atypical squamous and glandular cells of undetermined significance (ASCUS and AGUS), and infectious benign cellular change(BCC) diagnoses when compared to the CP. The ASCUS:LSIL ratio was reduced by 43% in the TP. Conclusions : A comparative analysis of the results from this study indicates that at least the TP is comparable to the CP for the detection of cervical lesions while providing the advantage of improved quality of specimens and increased detection rates for cervical abnormalities without requiring changes in current cytologic screening practices. Possible explanations for the increased number of inadequate specimens with the TP in this study are increased frequency of scanty cellularity due to split-sampling method, inadequate transfer of the cells from the sampling devices into the preservative solution and/or inadequate technical ability to collect the sample.
Kim, Eun‐,Mi,Kim, Jaehi,Kim, Yun‐,Gon,Lee, Peter,Shin, Dong‐,Sik,Kim, Mira,Hahn, Ji‐,Sook,Lee, Yoon‐,Sik,Kim, Byung‐,Gee John Wiley Sons, Ltd. 2011 Journal of peptide science Vol.17 No.5
<P><B>Abstract</B></P><P>Identification of substrate specificity of kinases is crucial to understand the roles of the kinases in cellular signal transduction pathways. Here, we present an approach applicable for the discovery of substrate specificity of Ser/Thr kinases. The method, which is named as the ‘high‐throughput phosphorylation profiling (HTPP)’ method was developed on the basis of a fully randomized one‐bead one‐compound (OBOC) combinatorial ladder type peptide library and MALDI‐TOF MS. The OBOC ladder peptide library was constructed by the ‘split and pool’ method on a HiCore resin. The peptide library sequence was Ac‐Ala‐X‐X‐X‐Ser‐X‐X‐Ala‐BEBE‐PLL resin. The substrate specificity of murine PKA (cAMP‐dependent protein kinase A) and yeast Yak1 kinase was identified using this method. On the basis of the result, we identified Ifh1, which is a co‐activator for the transcription of ribosomal protein genes, as a novel substrate of Yak1 kinase. The putative Yak1‐dependent phosphorylation site of Ifh1 was verified by <I>in vitro</I> kinase assay. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.</P>
Lee Tae-Gee,Yeum Dong-Min,Kim Young-Sook,Yeo Saeng-Gyu,Lee Yong-Woo,Kim Jin-Soo,Kim In-Soo,Kim Seon-Bong The Korean Society of Fisheries and Aquatic Scienc 2005 Fisheries and Aquatic Sciences Vol.8 No.2
A peptide that inhibits angiotensin-converting enzyme (ACE) was isolated from a hydrolysate of Manila clam (Ruditapes philippinarum) proteins prepared with thermolysin. Amino acid sequence of the peptide was determined to be Leu-Leu-Pro. Chemically synthesized Leu-Leu-Pro had an $IC_{50}\;value\;of\;158\;\mu{M}$. Peptides related to the Manila clam-derived peptide were synthesized to study the structure-activity relationships. The tetrapeptide, Leu-Leu-Pro-Pro, had a very weak effect on the enzyme. However, Leu-Leu-Pro-Asn showed no inhibitory activity.
신규 백금착물 항암제 KBP31705-C127 , KBP30603-901 의 Clsplatn 및 Carboplatin 과의 약동력학적 동태 비교
정인숙(In Sook Jung),이주선(Ju Seon Lee),허수정(Soo Jung Huh),김진숙(Jin Sook Kim),진창배(Chang Bae Jin),김동현(Dong Hyun KIm),김명수(Myung Soo Kim),박경수(Kyung Su Park),손연수(Youn Soo Sohn),백형기(Hyoung Gee Back),조양하(Yang Ha C 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.4
The present study examined pharmacokinetic profiles of KBP31705-C127 and KBP30603-901, new platinum coordination complexes synthesized as anticancer candidates, in comparison with two well-known platinum-containing anticancer agents, cisplatin and carboplatin in rats. Under sodium pentobarbital anesthesia of male Sprague-Dawley rats, urinary bladder, and femoral artery and vein were catheterized for urine collection, blood sampling and drug injection, respectively. Following i.v, administration of cisplatin (2 ㎎/㎏), KBP31705-C127 (2 ㎎/㎏), carboplatin (20 ㎎/㎏) or KBP30603-901 (20 ㎎/㎏), blood samples were collected at 2, 4, 6, 8, 10, 15, 20, 30, 45, 60 and 120 minutes. Urine samples were collected at 1-hr interval for 4 hr. Platinum concentrations in plasma and urine were measured using an inductively coupled plasmamass spectrometer. The plasma concentration-time curves were biphasic for all drugs during the time period studied. Compared with cisplatin, KBP31705-C127 showed similar decay patterns in the alpha- and beta-phases with slightly lower plasma concentrations. Urinary platinum excretion for cisplatin and KBP31705-C 127 was 56 and 52% of the administered dose in 4 hr, respectively. With regard to carboplatin and KBP30603-901, a similar decay pattern was also observed in the alpha-phase. The half life of KBP30603-901 in the beta-phase, however, was much longer than that of carboplatin, which was consistent with the urinary excretion results that 46 and 59% of the administered dose were excreted in the urine in 4hr, respectively. The results suggest that platinum coordination complexes are primarily excreted via the renal route and KBP30603-901 can elicit longer duration of action due to slower renal excretion compared to carboplatin.