Streptomyces Genome Engineering for Biosynthesis and Regulation of a Novel Immunosuppressant Linear Polyketide, Tautomycetin

Eung?Soo Kim 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

통조림용 가다랑어육의 식품성분

오광수,김정균,김인수,이응호,김복규 國立統營水産專門大學 附設 水産科學硏究所 1990 수산과학연구소보고 Vol.2 No.-

가다랑어 혈합육을 보다 효율적으로 이용하기 위한 일련의 기초연구로서, 가다랑어 혈합육의 지질성분, 함질소엑스분 및 구성아미노산 등을 보통 육과 함께 분석하여 비교, 검토하였다. 보통육은 혈합육에 비해 수분, 조단백질 함량은 약간 많은 반면, 조지방과 회분 함량은 혈합육이 약간 많았다. 휘발성염기질소량은 보토육이 22.7mg/100g, 혈합육이 46.9mg/100ㅎ으로 혈합육이 보통육에 비해 선도저하가 훨씬 빨랐다. 보통육과 혈합육의 총지질 조성은 각각 중성지질 79.7%, 71.9%, 당지질, 6.8%, 9.5%, 인지질13.5%, 18.6%로 이루어져 있었다. 중성지질의 성분은 free fatty acid ac triglyceride, 인지질은 phosphatidyl ethanolamine, phosphatidyl, 인지질은 phosphatidyl choline이 주성분이었고, lysogud 인지질의축적도 확인되었다. 총지지르 중성, 당 및 인지질의 축적도 확인되었다. 종지질, 중성, 당 및 인지질의 지반산 조성에서 보통육은 16:0 및 18:1을 주체로 한 포화산 및 모노엔산의 조성비가 혈합육보다 높았으며, 혈합육은 폴리엔산, 특히 22:6의 비율이 월등히 높았다. 16:0. 18:0, 18:1, 20:5 및 22:6 등이 주요 구성지방산이었다. 유리아미노산의 조성은 총함량에서 보통육 쪽이 많았고, 보통육에는 histidine, 혈합육에는 tauring의 보통육에서는 680.9mg.100g 정도 전존해 있었으나, 혈합육에서는 IMP의 분해가 급속히 진행되어 73.1mg/100g에 지나지 않았다. TMAO 및 TMA는 보통육에 비해 혈합육에 휠씬 많이 함유되어 있었고, total creatinine은 보통육 쪽이 많았다. 구성아미노산의 조성은 양시료 간에 별차이가 없었고, glutamic acid, aspartic acid, lysine, leucine, alanine, isoleucine 및 arginine 등이 주요 구성아니노산이었다. The lipid components, nitrogenous extracts and amino acids of dark muscle(DM) of skipjack (Katsuwonus pelamis) were analyzed and compared with those of white muscle(WM). WM was higher in moisture and crude protein content, and lower in crude lipid and ash content than those of DM. Contents of volatile basic nitrogen in WM and DM were 22.7mg/100g and 46.9mg/100g. Total lipid(TL) of WM and DM consisted of 79.7%, 71.9% neutral lipid(NL), 6.8%, 9.5% glycolipid(GL), and 13.5%, 18.6% phospholipid(PL), respectively. NL was mainly composed of free fatty acid, triglyceride, and PL was mainly occupied by phosphatidyl ethanolamine, phosphatidyl choline. Also lysophosphatidyl choline and lysophosphatidyl ethanolamine were identified in PL. In fatty acid composition of TL, NL, GL and PL, WM revealed higher contents in saturates and monoenes such as 16 : 0, 18 : 1, while DM showed higher contents in polyenes such as 22 : 6 especially. The major fatty acids of these samples were generally 16 : 0, 18 : 0, 18 : 1, 20 : 5 and 22 : 6. Contents of total free amino acids from WM and DM were 5,982.3mg/100g and 4,450.7mg/100g (dry base). Of free amino acids, Tau concentration was much higher in DM than in WM, Ala, Gly, Met, Arg, Thr were also high in DM. But His was much higher in concentration in WM. Content of inosinic acid(IMP) in WM(680.9mg/100g) was higher than that of DM(73.1mg/100g). The degradations of IMP proceeded very rapidly in DM. DM contained much higher trimethylamine oxide and trimethylamine than those of WM. The profile of combined amino acids in these samples were very similar, and main amino acids were Glu, Asp, Lys, Ala, Ile and Arg.

Poled Polymer를 이용한 제2고조파(SHG) 발생에 관한 연구

김응수 釜山 外國語 大學校 1998 外大論叢 Vol.18 No.2

A new learning method and control structure is presented for a neural network control system. The new learning index are composed of the identification error and control error, which is introduced to joint the learning error between the neural network controller and neural network identkfier. Also, the control law is derived by the neural network controller output and the compensated values which is represented the feedforward signal from the neural network controller. And we shows that the tracking performance and adaptability are demonstrated by simulation studies.

직장암 수술 후 국소재발과 예후

김태윤,백무준,김성용,신응진,박내경,이문수,김창호,송옥평 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Locally recurrent rectal cancer is a difficult clinical problem. Local recurrence following treatment for rectal cancer is a major cause of morbidity and mortality. The reported range of local recurrence rates following surgical treatment for rectal cancer has varied from 4% to 50%. We retrospectively reviewed the charts of 142 rectal cancer patients from January 1991 to December 1999. Analysis of patients factors included age, gender, type of surgery, and adjuvant therapy. Tumors were assessed for level, stage, and grade. Local recurrence and distant metastases were also documented. Variables influencing local recurrence in this group were identified and disease-free survival is determined. Of the 142 patients(74 males, 68 females; mean age 62.6 years), 137 consecutive patients operated on for rectal cancer and followed up to December 2000 (range 23-118months) were analyzed. Local recurrence rate was 18.3%. Age and sex of patients, type of surgery, location of tumor in the rectum, size, morphology and grading of the tumor were all unrelated to the event under investigation. At Cox regression, the Dukes stage and the postoperative radiotherapy were the only independent prognostic factors for local recurrence, and Dukes stage (Hazard ratio=2.89, p<0.001) and local recurrence (Hazard ratio=3.31, p<0.01) were the only independent factors associated with improved survival.

중복 자궁 경부, 맹낭성 종질중격 및 동측 신장발생부전을 가진 교통성 중복자궁 1례

김윤진,전명권,이응수,최형민 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

The class of uterine malformation known as communicating uteri is characterized by two uterocervical cavities connected by an isthmic communication. We experienced case of communicating double uterus with double cervix, blind hemivagina and ipsilateral renal agenesis. The complaint of the patient was odorous vaginal discharge with lower abdominal pain unrelated with menstrual cycle. We confirmed these Mullerian anomalies by transvaginal ultrasound. IVP(intravenous pyelography), MRI(magnetic resonance imaging), hysterosalpingography and hysteroscopy. After vaginal septoplasty, her complaint was subsided. We report this case with brief review of literatures.

일단계 핵산 추출을 이용한 사람거대세포바이러스와 돼지내재레트로바이러스의 검출법

김정헌,정은숙,황응수 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Xenotransplantation is thought to be one of the alternative methods to overcome the shortage of human organs for transplantation. Recipients should be immunosuppressed for graft survival, and thus, there is a need for developing diagnostic modality that can detect diverse infections originating from animals and recipients rapidly, in the early stage, and with high sensitivity using small volume of samples. This study was carried out to develop a fast, simple, and robust technique for the preparation of HCMV DNA and PERV RNA using small volume of samples. Materials and Methods: Nucleic acids were extracted from serially diluted samples with one step extraction method as well as with Qiagen kit. The presence of genomic DNA of human cytomegalovirus (HCMV) and porcine endogenous retrovirus (PERV) was detected by PCR and specific primer set, respectively. RNA of HCMV and PERV was extracted and then detected by RT-PCR and specific primer set, respectively. For absolute quantification of HCMV, standard curve was established by real time PCR. Results: HCMV DNA and PERV RNA were prepared from culture supernatant and cells for PCR or RT-PCR with one step extraction method. It was possible to extract both the DNA and RNA from the samples in about 20 minutes with one step extraction method in a single tube. HCMV and PERV could also be detected by PCR and one step extraction method, respectively. It was also good with small quantity samples. Conclusions: One step extraction method is simpler and faster method than other extraction methods when there are two types of DNA and RNA viruses in one sample. From these results, we could see that the one step extraction method could be very useful in detecting HCMV and PERV rapidly from the pig cells or organ transplanted recipients with a small amount of sample.

택지개발지구내 도시공원의 분포특성에 관한 연구

김수봉,정응호,김정환,류지원 한국정원학회 2002 한국전통조경학회지 Vol.20 No.2

To generate a suggestion for a better framework especially the distribution and locations of urban parks in residential areas, ‘residential site development districts’ in Daegu city were tested based on the community involvement and user’s demand for visting. Service-range, advantage and disadvantage in use derived from the locations of the parks in the districts were analyzed according to the concept of density,character assessment, structure in the distribution and locations. Suggestions to give residents more friendly surroundings with ’easy access’ and ’friendly characteristics of each parks’ are as follows; ⅰ) Although most of the tested sites are satisfying the legal criterion on the required level of size of urban parks in each district, there are big difficulties to have rich natural environment in the case of extensive size of residential sites due to too low applied-criterion(0.6㎡ per person) which is a minimum requirement. ⅱ) Although the rate of size of parks in the sites were gradually improved recently, the portion for each person had not improved at all. Therefore, it is needed that new detailed criteria in different level rooted in the character of individual sites such as population and characteristics of parks. ⅲ) Although the distance of parks is within the range of use, the locations are on the edge of sites and residential districts where is not on the everyday route. Therefore to give more easy accessibility, the urban parks in the residential sites should be located on the daily life route where is a more common area in future plans.

도시열섬현상 저감을 위한 그린네트워크 구축 방안에 관한 연구 : 대구광역시 달서구를 대상으로

김기호,김수봉,정응호 한국환경과학회 2004 한국환경과학회지 Vol.13 No.6

As urbanization has been expanded in Korea, open spaces, such as urban parks and public sites, have been utilized for other uses, and then this bring out environmental changes for the worse. It is expected that these changes become more serious problems due to overpopulation, increasing individualism, and development of transportation. This research base on the consideration of connecting between decreasing urban green spaces and distributed green sites so as to build the substantial plan for the Green Network construction for urban heat island mitigation in Dalseo district, Daegu Metropolitan City. The result were as follow; 1) Connecting existing natural sites to the remained parks green zone made an Ideal form of Green-Network system. 2) Some school sites were selected for usable open spaces in order to build Green-Network system, and the plan connecting together with exist natural sites was suggested. 3) Moreover, the scheme of planting on the road spaces for connecting green spaces was proposed. 4) The devices of planting on the urban riverside for enhancing the role of urban stream to form green network was conceived.

인대내 거대자궁 근종 증례

김윤진,전명권,이응수,최형민 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Uterine myomas are the most common pelvic tumor in women. These benign tumor generally arise in the uterinie wall and intraligamentary myomas are relatively rare cases. We experienced intraligamentary huge myoma. Preoperative GnRH(Gonadotropin Releasing Hormone) agonist therapy was done but the effect was nearly absent. Hystrectomy was done without complication. We report this case with brief review of literatures.

극박 oxide막의 전기적 특성에 영향 미치는 다결정 실리콘 게이트의 열처리효과

김응수 부산 외국어 대학교 2001 外大論叢 Vol.22 No.1

The annealing effects on micro-structures and electrical properties of amorphous silicon gate and of thin gate oxide were observed.The amorphous silicon gate was crystallized by post gate anneal.The resistivity of gate was decreased due to the increse of dopant activation rate as increasing the annealing temperature.The electrical properties of thin gate oxide are improved by post gate anneal at higher temperatures.Particularly, the interface trap density at oxide/silicon decreases with the increase of annealing temperature.