HSV-TK 유전자를 암호화하는 EBV 유래 플라스미드를 이용한 유전자 치료

오상택,민경아,김종국,이숙경 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.4

Herpes simplex virus (HSV) thymidine kinase (TK) has been widely used for suicidal gene therapy in combination with nucleoside analogs such as ganciclovir (GCV). The use of HSV-TK is limited due to the side effect of GCV at high concentraions. Previous studies showed that stable transfectants of mustant HSV-TK with enhanced affinity to GCV were killed at lower GCV concentrations. In this study, we tested whether mutant HSV-TK can provide enhanced suicidal effect when trasiently transfected with Epstein-Barr virus (EBV)-based plasmid. EBV-based plasmid which contains Orip and EBNA-1 sequence is well known for a stable espisomal maintenance in human cells. Optimal transfection condition was assessed for SNU-638 gastric cancer cell line using polyethylenimine (PEI). Maximum transfection efficiency was achieved when DNA:PEI was 1:3 (w/v). Cytotoxicities of mutant and wild type HSV-TK were compared before and after partially selecting transfected cells. The were sensitive to 100?/ml hygromycin. Following GCV treatment, more cells were killed after hygromycin selection than before selection. The mutant HSV-TK showed enhanced cytotoxicity compared with the wild type HSV-TK. Our results suggest that the EBV-based plasmid encoding mutant HSV-TK may be useful to treat the diseases caused by uncontrolled cell proliferation such as cancer and rheumatoid arthritis.

釜山地域 經濟發展을 위한 地域企業人의 役割과 責任 : 企業家精神과 地域的 責任을 中心으로

오종석,황한식,김종관 부산대학교 1995 地域經濟硏究 Vol.- No.4

한국경제는 지금까지 국가 중심적 경제체제를 유지해 왔으나, 세계화, 지방화라는 시대적 변화에 직면하여 지역 분권적 경제체제를 확립하고, 이를 통해서 지역경쟁력을 강화함으로서 지역중심의 세계화를 추진해 나가지 않을 수 없게 되었다. 따라서 본 연구는 효과적인 지역경제발전을 모색해 나가기 위해서 부산지역을 연구대상으로 하여, 지역경제발전의 주체와 지역경제발전의 원동력을 조명하고, 지역경제발전을 위해서는 어떠한 환경이 조성되어야 하는 지를 문헌연구와 실증연구를 토대로 하여 고찰해 보고자 하였다. 그 결과, 다음과 같은 결론을 도출할 수 있었다. 첫째, 세계화, 지방화 시대의 지역경제 발전전략은 자립적·내발적 이어야 하며, 지역 스스로가 발전주체가 되어야 한다는 점이다. 특히 지역기업인은 지역에서 가장 중심적인 역할을 담담해야 하며, 지역기업 중심의 지역주체성 확립이 지역경제 발전의 기반이 되어야 한다는 것이다. 둘째, 지역경제 발전의 원동력은 지역기업인의 기업가정신과 지역적 책임이며, 이것을 통해 기업의 내생력을 강화시켜서 기업발전을 이룩하고, 지역경제의 발전을 실현해 나가야 한다는 점이다. 셋째, 지역경제의 발전을 위해서는 지역경제의 낙후원인을 분석, 진단하고 이를 통해서 외생적 환경조건을 정비함으로써 지역경제의 발전을 도모해 나가야 한다는 것이다.

사용자 MMI 화면 구성 방식의 DB 시스템 개발

오원록,김종교 全北大學校 1995 論文集 Vol.39 No.-

One of the most prominent feature of our database system is that it uses the User MMI screen layout and input record structure which users construct. Therefore, programmers need not program source code for each application area. Users can take effects of programming applications by just screen editing and input data record definition. The database system consistes of 3 program modules. 1) screen editor(sedit) - editing the initial screen for the DB engine, 2) form editor(form) - defining data record structures for DB engine, 3) the DB engine(playform) - managing database of the structure the user has defined. We will evolve this DB system to graphic mode, multi-key system, develop several db utilities, such as sort, print, etc. By using this system, users themselves can design their own db system(User screen, data record structure) easily, just as they develop DB application programs.

과학정책을 위한 정부조직의 역사적 개관

吳鎭坤,金鍾一 全北大學校 1996 論文集 Vol.41 No.-

The role of govements for promotions of science and science policy at national level originates from the initiatives which began in the seventeenth century in several European countries. We present a brief historical survey of government organizations for science policy and for promotion of science.

정보기술의 특성이 조직구성원의 저항에 미치는 영향에 관한 연구 : 사용자의 IT인지정도와 개인성향을 중심으로

오종석,김경원 釜山 大學校 經營 經濟 硏究所 2002 經營 經濟 硏究 Vol.21 No.1

정보기술 도입과 관련된 연구에 있어서 초기의 시스템설계나 시스템관리와 같은 기술적 측면에서 접근하였으나 최근 연구에서는 조직구조나 조직 행동적인 측면에서 접근하려는 시도가 많아지고 있다. 정보기술의 도입은 조직의 변화를 유발하며 조직구조의 변화는 조직구성원의 반발과 저항을 초래할 수 있기 때문에 본 연구를 통하여 정보기술의 도입과 저항과의 관계를 조직행동적 측면에서 고찰하고자 한다.

現代 科學技術의 基本的 理念

吳鎭坤,金鍾一 全北大學校 科學敎育硏究所 1996 과학과 과학교육 논문지 Vol.21 No.-

Science and technology, by virtue of efficiency and rationality, today opens up a variety of new fields for human and social activity, and operates as a main driving force of social development. This work briefly reviews several important concepts related to science and technology which will eventually determine the future of human civilization.

封着유리의 製造에 關한 硏究

金鳳杰,金宗範,權昶吾,朴慶煥 慶熙大學校 1988 論文集 Vol.17 No.-

The purpose of these studies are to prepare low melting devitrifiable solder glass for high termal expansion seal and to find out solder glass which is the optimum condition in terms of thermal properties among others. Then five kinds of frits were prepared by quenching after melting of solder glass in the Pt-crucible at 1,100℃, which contained 2wt% of ZrO₂ as a neucleation agent with being control led by the concentration of mother glass of PbO-ZnO-B₂O₃-SiO₂ system. For probing the characteristics of the solder glass, DTA, X-ray powder diffraction, thermal, expansion, sealability, fluidity and activation energy were examined. The results are as follows: 1. It was possible in sealing of low temperature that the sealability & crystallization temperature was lowered and the fludity was good owing to an increase in PbO wt% but it was inadquate in sealing of color TV tube for 80.5wt% PbO and over because thermal expansion was increased. 2. The optimum holding time was 60 minutes. 3. In the sample contained 80.5wt% PbO, the thermal expansion coefficient was 101.8×10^-7/℃, the crystallization temperature was 443℃, flow button diameter was 27.7㎜, the sealability was 25.15㎏f/㎠, It is better than the existing solder glass. 4. The crystals proved in process of melting and crystallization were Pb_8Zn(Si₂O_7)₃, PbZrO, Zn₂SiO₁ etc. 5. The activation energy needed for crystallization was 87.13kJ/mol.

아세톤-물 혼합용매내에서 1_1 전해질의 전기전도도에 대한 연구

오형식,김종현,전동철 木浦大學校基礎科學硏究所 1988 基礎科學硏究誌 Vol.6 No.-

여러가지 조성의 아세톤-물 혼합용매중에서 LiC1, LiBr 및 Lil의 희박용액 전도도를 25℃에서 측정하였다. 그 결과 각 전해질들의 여러 농도에 따르는 당량전도도의 변화가 Fuoss-Onsager's semiem-pirical 식에 대해서 좋은 일치를 보여 주었다. 또한 아세톤-물 혼합용매의 몰 분율이 증가함에 따라 극한당량전도도??값이 LiC1, LiI는 0.3 몰 분율에서, LiBr는 0.2 몰 분율에서 가장 작은 값을 나타내었다. Walden Product??는 LiC1와 LiBr가 0.1 몰 분율에서, LiI는 0.0 몰 분율에서 최대치를 보여 주었고, 그 후로는 아세톤 몰 분율의 증가에 따라 계속 감소하였다. The equivalent conductance of LiC1, LiBr and LiI have been measured in Acetone-Water mixtures of various composition (0.0∼0.6 mole fraction) at 25℃. The value of equivalent conductance agreed well with the Fuoss-Onsager's semiempirical equation. The minimum limiting equivalent conductance was found in 0.3 mole fraction Acetone for LiC1, LiI and LiBr was found in 0.2 mole fraction Acetone. The maximum Limiting Walden Products?? of LiC1 and LiBr was found in 0.0 mole fraction Acetone, and LiI was found in 0.0 mole fraction Acetone. And also, after 0.1 mole fraction and 0.0 mole fraction, decreased with increasing of mole fraction Acetone.

한국어 표준 음성 데이타베이스 구축

오원록,김종교 全北大學校 1995 論文集 Vol.39 No.-

The main goal of this research is as follows: 1. To make a Korean standard telephone speech database composed of widely used and phonetically-balanced speech items. 2. To help associated universities and laborarories from making speech materials which is essential to speech processing studies by distributing the speech database to them. 3. To provide performance criteria for speech analysis and recognition algorithms. In the last year, we surveyed research trends in foreign countries, designed file formats for speech corpus, prepared for speech database collection/trimming, and made a useful Korean telephone speech database.

정상인과 뇌졸중환자에서 운동유발전위의 평가

김동현,박종현,권오상,김두응 중앙대학교 의과대학 의과학연구소 1997 中央醫大誌 Vol.22 No.2

Since the development of magnetic stimulation as a way of assessing the function of central motor pathways, various neurologic diseases have been investigated by motor evoked potentials(MEP). This study was carried out to evaluate the usefulness of clinical application of MEP to the stroke patients. MEP were elicited with transcranial cortical stimulation using a magnetic coil in 21 healthy subjects and 18 stroke patients. Average of age was 37.5±18.0 and 63.7±7.7 years in normal and patient groups respectively. All stroke patients had a mild to severe clinical unilateral motor weakness, and also were proven to have unilateral infarction or hemorrhage at various regions of brain radiologically. During MEP studies, neither significant side effects nor pin were encountered. MEP latency of abductor pollicis brevis(APB) and extensor digitorum brevis(EDB) in controls measured 22.4±2.0 msec and 41.8±2.9 msec respectively. Normal central motor conduction time(CMCT) was 9.7±1.6 msec and 19.3±2.3 msec respectively. The amplitudes of MEP were not reliable due to great variability. Abnormal responses were noted in 5 patients on the APB and in 12 patients on the EDB motor evoked potentieal studies. Mean latency and CMCT were significantly prolonged in involved sites of the other 16 patients, comparing with control data. Prolonged latency and CMCT, increased inter-side difference of CMCT. Any potential was not a useful indicator in determining abnormality of MEP study. More paralyzed patients were apt to show abnormal responses in MEP studies. There were also more abnormal results during acute stage compared with chronic stage More abnormal results were observed in the patients with cerebral cortex or corona radiata involvements. It is suggested that MEP study is a useful and sensitive method in the evaluation of stroke patients and be a effective method for further understanding of central motor system.