Comparison of trophic factors changes in the hippocampal CA1 region between the young and adult gerbil induced by transient cerebral ischemia.

Yan, Bing Chun,Park, Joon Ha,Kim, Sung Koo,Choi, Jung Hoon,Lee, Choong Hyun,Yoo, Ki-Yeon,Kwon, Young-Geun,Kim, Young-Myeong,Kim, Jong-Dai,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2012 Cellular and molecular neurobiology Vol.32 No.8

<P>In the present study, we investigated neuronal death/damage in the gerbil hippocampal CA1 region (CA1) and compared changes in some trophic factors, such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF), in the CA1 between the adult and young gerbils after 5 min of transient cerebral ischemia. Most of pyramidal neurons (89%) were damaged 4 days after ischemia-reperfusion (I-R) in the adult; however, in the young, about 59% of pyramidal neurons were damaged 7 days after I-R. The immunoreactivity and levels of BDNF and VEGF, not GDNF, in the CA1 of the normal young were lower than those in the normal adult. Four days after I-R in the adult group, the immunoreactivity and levels of BDNF and VEGF were distinctively decreased, and the immunoreactivity and level of GDNF were increased. However, in the young group, all of their immunoreactivities and levels were much higher than those in the normal young group. From 7 days after I-R, all the immunoreactivities and levels were apparently decreased compared to those of the normal adult and young. In brief, we confirmed our recent finding: more delayed and less neuronal death occurred in the young following I-R, and we newly found that the immunoreactivities of trophic factors, such as BDNF, GDNF, and VEGF, in the stratum pyramidale of the CA1 in the young gerbil were much higher than those in the adult gerbil 4 days after transient cerebral ischemia.</P>

노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

Development of High efficiency Food Waste Treatment System for Entering New and Renewable Energy Industry in Southeast Asia

Kwon,Ki-Tae,Lee,Woo-Sik,Kwon,Lee-Seung,Seong,Seung-Hwan,Kim,Young-Do,Kwon,Woo-Taeg 한국유통과학회 2019 KODISA ICBE (International Conference on Business Vol.2019 No.-

It is possible not only to treat high concentration organic matter such as food wastes but also to utilize food wastes as resources according to the bio-energy generation such as methane. The method of co-digestion of mixed wastewater mixed with organic wastes such as sewage sludge concentrated waters together with drinking wastewater is the most ideal method. Radiation not only has germicidal power in water treatment, but also is effective in decomposing degradable organic matter. Second chemical contamination problem is also solved because it does not use chemicals. Introduction of radiation pretreatment system to solve problems caused by mixing of waste water and organic waste resources. Biological electrochemical fusion system is used to remove the nutrients contained in the organic matter-removed mixture. Through the development of a system for treatment of high concentration wastewater, the processing efficiency is verified through the universal water treatment system for wastewater containing high concentration organic matter in addition to treatment of wastewater.

Biochemical Characteristics and Antimicrobials Susceptibility of Salmonella gallinarum Isolated in Korea

Ki-seuk Kim,Young-ju Lee,Yong-kuk Kwon,Ryun-bin Tak 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Characteristics and Antimicrobials Susceptibility of Salmonella gallinarum Isolated in KoreaYoung-ju Lee, Ki-seuk Kim1,*, Yong-kuk Kwon and Ryun-bin Tak1Avian Disease Division, National Veterinary Research and Quarantine Service, Anyang 430-824, Korea1College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, KoreaReceived February 13, 2003 / Accepted April 30, 2003J. Vet. Sci. (2003), 4(2), 161-166JOURNAL OFVeterinaryScience*Corresponding author: Ki-seuk Kim College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea Tel: 82-53-950-5962, Fax: 82-53-950-5955 E-mail: kimkiseuk@knu.ac.kr

中風病辨證診斷標準에 의한 臨床的 硏究

任長信,文祥官,高昌男,權正男,曺基湖,金永錫,裵亨燮,李京燮 경희대학교 1997 慶熙醫學 Vol.13 No.3

삼풍백화점 붕괴사고시 혈액형의 결과와 유가족 진술과의 비교고찰

권기석,김영주 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

취학전 아동의 체력 실태와 평가

권영옥,김동권,민춘기,이용인,전종귀,진윤수,홍성표 忠南大學校體育科學硏究所 1995 體育科學硏究誌 Vol.13 No.1

The purpose of this study was analyze children's Physical Fitness and basic rate by observing the change in the heart rate of the 1230 children of five of six years of age. Following conclusions were obtained The muscular strength was relatively low according to places. And the muscular strength of boys was higher than that of girls. The flexibility of girls was higher than that of boys according to places and sexes. The muscular endurance, power, balance and agility of boys were higher than those of girls according to places and sexes. Thus the development of physical fitness was improved with their ages, the physical fitness for physique seemed rather low. So the programs of physical plays for children's physical fitness ought to be developed and physical play showed to be done at kindergarten school.

전위된 상악 견치 및 하악 측절치의 치험례

권해숙,현홍근,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4

Tooth transposition is an anomaly of eruption characterized by the interchanged positions of two adjacent teeth, which is divided into complete and incomplete transposition. There are three common approaches for treating transposition: aligning the involved teeth in their transposed positions, moving them to their correct anatomic position in the arch and extracting one of the transposed teeth. Considerations in treatment plans are esthetic, function, risk of jeopardizing the roots and damaging the supporting structures, position of the root apex, developmental stages of teeth and expected compliance. The presented case reports described one maxillary canine-the first premolar transposition and two mandibular lateral incisor-canine transpositions. The former transposed teeth were arranged in their transposed position, and the latter transposed teeth were rearranged into their normal position. 치아전위란 인접한 두 치아의 위치가 서로 바뀐 치아 위치 이상을 말하며, 치관과 치근의 위치에 따라 완전전위와 불완전전위로 나눌 수 있다. 하악보다는 상악에 편측성으로 호발하며, 특히 상악 견치에서 자주 발생한다. 전위는 발견 시기와 전위된 양상에 따라 치료방법이 달라질 수 있다. 본 증례는 상악 견치와 제1소구치 간의 전위가 일어난 10세 7개월의 환아와 하악 측절치와 견치 간의 전위가 일어난 8세 11개월 환아와 8세 9개월 환아의 교정치료에 대한 것으로 양호한 치료 결과를 얻었고, 증례 별로 치료방법의 차이점에 관한 고찰을 하였기에 이를 보고하는 바이다.

유치 수복물에 따른 주변 법랑질의 탈회 저항성 : QLF를 이용한 연구

권해숙,현홍근,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

본 연구에서는 유치 수복물의 불소 방출 여부에 따른 주변 법랑질의 탈회 저항성과 재광화 효과를 살펴보고자 하였다. 건전 유전치 48개를 16개씩 임의로 세 군으로 나누어, FiltekTM Z250(1군), F2000(2군), KetacTM N100(3군)을 사용하여 제조사의 지시에 따라 충전하였다. 3일 동안 인공 우식 병소를 유발한 후, 14일 동안 인공 타액에 담가 재광화를 유발하였다. Quantitative light-induced fluorescence(QLF)를 이용하여 실험 단계와 시간에 따른 무기질 소실량(ΔQ)의 변화를 관찰한 결과는 다음과 같다. 1. 탈회가 일어난 인공 우식 병소의 ΔQ 값은 3군, 2군, 1군 순으로 작게 나타났고, 이 중 3군은 1군에 비해 유의한 탈회 저항성을 보였다. 2. 1군, 2군, 3군 모두 재광화 1일 후부터 초기 인공 우식 병소의 ΔQ 값과 비교 시 유의한 차를 보였으며, 관찰 기간 동안 지속적인 증가 양상을 나타내었다. 3. 재광화 속도를 의미하는 Δ(ΔQ)/일 값은 각 군에서 처음 1일 동안 유의성 있게 크게 나타났으며, 그 이후 급속히 감소하였다. 4. 수복 재료에 따른 재광화 정도는 각 군 간에 유의한 차이가 없었다. The objective of this in vitro study was to detect and monitor demineralization and remineralization of primary teeth according to restorative materials using quantitative light-induced fluorescence (QLF). A single bur hole was drilled on the each sound forty eight primary anterior teeth, and the specimens were divided into three groups. The cavity was restored with FiltekTM Z250(Group 1), F2000(Group 2), KetacTM N100(Group 3) following the manufacturer's instructions. The teeth were subjected to the demineralizing buffer for 3 days, and then subjected to a remineralizing buffer for 14 days. The change of mineral loss(ΔQ) according to the stages was evaluated by QLF and the following results were obtained: 1. When demineralization was done, ΔQ was increased as follows. : Group 1 (-110.79 ± 27.77) < Group 2 (-104.84 ± 28.95) < Group 3 (-90.16 ± 21.87) : Resistance to demineralization was statistically significant in Group 3. 2. There was a statistically significant increase in ΔQ of all groups since 1st day of remineralization 3. The rate of remineralization, Δ(ΔQ)/day, showed significant high value in each group on the 1st day then decreased rapidly. 4. There was no statistically significant difference in the degree of remineralization among restorative materials.

월슨씨병에서 나타난 안검개방실행증 1예

박기종,권오영,최낙천,김남곤,김태유,임병훈 대한신경과학회 1997 대한신경과학회지 Vol.15 No.5