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Chinese hamster 난소세포에서 수용성 니켈에 의해 특징적으로 발현되는 유전인자들의 빠른 분석에 관한 연구
성근제,이상한,우기민,송호연,조만희 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2
니켈에 의한 암 발생기전 및 세포 독성기전을 이해하고 니켈에 의해 발현에 변화를 보이는 유전자들을 검출하기 위하여 니켈이 포함된 배지에서 배양된 Chinese hamster난소 세포부터 mRNA differential display 분석을 시행하였다. 본 연구에서는 동위원소나 DNA염기서열 분석용 젤을 사용하는 기존의 방법 대신에 훨씬 빠르고, 다루기 쉽고 안전성을 지닌 변형된 기법을 개발하여 적용하였다. 니켈에 의해 발현에 변화를 보이는 cDNA fragments들을 10% polyacrylamide mini젤상에서 분리 및 클로닝한 다음, DNA염기 서열 분석과 GenBank Blast search 프로그램을 이용하여 상동성 조사를 하였다. Reverse Northern blot을 이용하여 발현에 변화가 있는 cDNA들을 재확인한 결과, 검사된 19 cDNAs 들중 총 9종에서 발현의 변화를 보였으며, 이 중 3종은 니켈에 반응하여 발현에 증가를 보였고, 반면에 6종은 발현이 감소하였다.
풍선 확장술로 치료한 담관결석과 동반된 총담관 막양구조
백성민 ( Sung Min Baek ),김동욱 ( Dong Uk Kim ),장선미 ( Sun Mi Jang ),송병구 ( Byeong Gu Song ),박종만 ( Jong Man Park ),이대성 ( Dae Sung Lee ),김중근 ( Joong Keun Kim ),황경림 ( Kyung Lim Hwang ) 대한췌장담도학회 2014 대한췌담도학회지 Vol.19 No.3
Bile duct web is very rare disease and it`s etiology is controversial. Some webs are occurred in the presence of chronic inflammation, frequently associated with bile duct stone, but others are thought to be congenital. Many patients with bile duct web are asymptomatic, but they sometimes present symptom of biliary obstruction and cholangitis. It can be diagnosed by endoscopic retrograde cholangiopancreatogram, typically appearing as thin and shelf like radiolucent ring. We report a case of the common bile duct web with bile duct stones diagnosed by Endoscopic retrograde cholangiopancreatography (ERCP) in a 65-year-old man. The patient was treated by balloon dilatation successfully.
Song, Keun Man,Kim, Do-Hyun,Kim, Jong-Min,Cho, Chu-Young,Choi, Jehyuk,Kim, Kahee,Park, Jinsup,Kim, Hogyoug IOP Pub 2017 Nanotechnology Vol.28 No.22
<P>We demonstrated an InGaN/GaN-based, monolithic, white light-emitting diode (LED) without phosphors by using morphology-controlled active layers formed on multi-facet GaN templates containing polar and semipolar surfaces. The nanostructured surface morphology was controlled by changing the growth time, and distinct multiple photoluminescence peaks were observed at 360, 460, and 560 nm; these features were caused by InGaN/GaN-based multiple quantum wells (MQWs) on the nanostructured facets. The origin of each multi-peak was related to the different indium (In) compositions in the different planes of the quantum wells grown on the nanostructured GaN. The emitting units of MQWs in the LED structures were continuously connected, which is different from other GaN-based nanorod or nanowire LEDs. Therefore, the suggested structure had a larger active area. From the electroluminescence spectrum of the fabricated LED, monolithic white light emission with CIE color coordinates of <I>x</I>?=?0.306 and <I>y</I>?=?0.333 was achieved via multi-facet control combined with morphology control of the metal organic chemical vapor deposition-selective area growth of InGaN/GaN MQWs.</P>
오세진,강상중,송수근,임동현,김성만,최영식,박요한,차형수 고신대학교 의학부 2000 高神大學校 醫學部 論文集 Vol.15 No.1
Background Obesity is the prevalent disorder and is a serious public health concern because it is a significant forerunner of major chronic debilitating metabolic disease including diabetes, hypertension, lipid disorders and coronary artherosclerosis. Recently many studies shows that upper-body obesity, particulary increased visceral, abdominal fat, is most strongly associated with metaboloc risk factors. The aims of this study were to establish the relationship of body fat and its distribution to metaboloc risk factors and the clinical usefulness of waist circumference as a new indices of intraabdominal fat distribution. Methods Forty-one non-diabetic patients who visited to the health promotion center of Kosin Medical Center from March, 1998 to June, 1998 were divided into two groups according to the body mass index (BMI) : obese group (BMI≥25㎏/m^(2)) and non obese group (BMI<25㎏/m^(2)) also were divided into two group according to the waist circumference (W) : center obese group (W>94㎝ in men and W>80㎝ in women) and central non onese group (W≤94㎝ in men and W≤80㎝ in women). The amount of body fat was measured by bioelectrical impedence method. Insulin resistance was calculated by homeostasis model assessment. Results The result were as follows. 1) In obese group W and waist/hip ratio (WHP). basal insulin level in men and Smin, C-peptide and atherogenic index in women of obese group were higher than those of non-obese group (p<0.05). Insulin resistance, fat weight and lean body mass in the obese group were higher than those of non-obese group in both sexes (p<0.05). 2) In central obese group BMI, basal insulin level, insulin resistance, fat weight and lean body mass in men and atherogenic index in women were higher than those of central non-obese group. WHR in the central obese type group was higher than of central non-obese group in both sexes (p<0.05). 3) BMI was positively correlated with systolic blood pressure (r=0.32, p<0.05), diastolic blood pressure (r=0.36, p<0.05), insulin resistance (r=0.39, p<0.05), waist circumference (r=0.61, p<0.05), percentage of body fat (r=0.32, p<0.05), fat weight (r=0.61, p<0.01) and lean body mess (r=0.54, p<0.01), but negatively correlated with HDL-cholesterol (r=0.76, p<0.01). 4) Waist circumference was positively correlated with systolic blood pressure (r=0.46, p<0.01), diastolic blood pressure (r=0.37, p<0.05), fat weight (r=0.37, p<0.05), lean body mass (r=0.37, p<0.05), and WHR (r=0.82, p<0.01). 5) WHR was not significantly correlated with other metabolic risk factors except systolic blood pressure (r=0.39, p<0.05). 6) Fat weight was positively correlated with diastolic blood pressure (r=0.36, p<0.05), insulin resistance (r=0.53, p<0.01), basal insulin level (r=0.53, p<0.01),and percentage of body fat. Percentage of body fat was positively correlated with diastolic blood pressure (r=0.33, p<0.05), and insulin resistance (r=0.44, p<0.01). Conclusion Conclusively body fat, waist circumference and WHR were positively correlated with metabolic risk factors and waist circumference appeared more useful than WHR.
Single Pleural Relapse of a Nasal-Type Extranodal Natural Killer/T-Cell Lymphoma: A Case Report
( Keun Mo Kim ),( Young Min Oh ),( Sung Nam Lim ),( Song Yi Choi ),( Ok Jun Lee ),( Kang Hyeon Choe ),( Ki Man Lee ),( Jin Young An ) 대한결핵 및 호흡기학회 2014 Tuberculosis and Respiratory Diseases Vol.76 No.4
A nasal-type extranodal natural killer/T-cell lymphoma is considered an aggressive form of non-Hodgkin`s lymphoma, with approximately half of all patients relapsing during the follow-up period, and most relapses occurring within the first 2 years of remission. Here we report an unusual case of a 42-year-old man who experienced recurrence in single pleura after 8 years of remission.
Efficiency enhancement in a-plane InGaN/GaN light emitters with carbon nanotubes.
Song, Hooyoung,Suh, Jooyoung,Kim, Eun Kyu,Shin, Kwonwoo,Han, Jong Hun,Hwang, Sung-Min,Song, Keun Man American Scientific Publishers 2012 Journal of nanoscience and nanotechnology Vol.12 No.4
<P>This study investigates the coupling modes of a-plane InGaN/GaN mutiquantum wells (MQWs) with single-walled carbon nanotubes (SWCNTs). The enhancement of light emissions at resonance photon energies can be explained by the surface plasmon coupling of the MQW-SWCNT hybrid structure. The photoluminescence (PL) enhancement ratios of the indigo (2.90 eV) emission from MQWs with SWCNTs reveal three coupling modes at 2.50 eV, 2.97 eV, and 3.42 eV. In addition, the trend of the PL intensity ratios and efficiencies corresponds to that of the PL enhancement ratios. The PL efficiencies for the green (2.46 eV) and indigo (2.90 eV) emissions of SWCNT-coated MQWs are 32% and 110% better than the corresponding values of uncoated MQWs, respectively. The results show that the MQW-SWCNT hybrid structure has the potential to be applied in high-efficiency light emitters in the visible and ultraviolet range.</P>
Song, Geun Cheol,Im, Hyunjoo,Jung, Jihye,Lee, Soohyun,Jung, Man‐,Young,Rhee, Sung‐,Keun,Ryu, Choong‐,Min BLACKWELL SCIENCE 2019 ENVIRONMENTAL MICROBIOLOGY Vol.21 No.3
<P><B>Summary</B></P><P>Archaea have inhabited the earth for a long period of time and are ubiquitously distributed in diverse environments. However, few studies have focused on the interactions of archaea with other organisms, including eukaryotes such as plants, since it is difficult to cultivate sufficient numbers of archaeal cells for analysis. In this study, we investigated the interaction between soil archaea and <I>Arabidopsis thaliana</I>. We demonstrate for the first time that soil archaea promote plant growth and trigger induced systemic resistance (ISR) against the necrotrophic bacterium <I>Pectobacterium carotovorum</I> subsp. <I>carotovorum</I> SCC1 and biotrophic bacterium <I>Pseudomonas syringae</I> pv. <I>tomato</I> DC3000. Ammonia‐oxidizing archaeon <I>Nitrosocosmicus oleophilus</I> MY3 cells clearly colonized the root surface of <I>Arabidopsis</I> plants, and increased resistance against both pathogenic species via the salicylic acid‐independent signalling pathway. This mechanism of bacterial resistance resembles that underlying soil bacteria‐ and fungi‐mediated ISR signalling. Additionally, volatile emissions from <I>N. oleophilus</I> MY3 were identified as major archaeal determinants that elicit ISR. Our results lay a foundation for archaea–plant interactions as a new field of research.</P>