Purification, characterization and gene cloning of metalloprotease from Nomuraea atypicola

Naomi Yamamoto,Mitsuhiro Ueda,Mizuho Kusuda,Masami Nakazwa,Kenji Ohuchi,Minoru Sakaguchi,Kuniyo Inouye,Kazutaka Miyatake 한국버섯학회 2010 한국버섯학회 국제학술대회 Vol.6 No.-

Fabrication and Properties of Double Perovskite SrLaVRuO6

Ryosuke Zenzai,Tetsuya Miyawaki,Kenji Ueda,Hidefumi Asano 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.3

Polycrystalline bulk and epitaxial thin film samples of double-perovskite SrLaVRuO6 were prepared,and their magnetic and electrical properties were investigated. Structural characterizationon the bulk samples has revealed that a double-perovskite phase can be formed in coexistence withthe secondary phase of nonmagnetic semiconducting Sr3V2O8. Magnetic and electrical investigationshave shown that the double-perovskite SrLaVRuO6 compound is an antiferromagnetic metal. Moreover, it has been found that epitaxy using a lattice-matched substrate leads to suppression ofthe Sr3V2O8 secondary phase and promoting growth of phase-pure double-perovskite SrLaVRuO6.

Poster Session : PS 0620 ; Others ; The Role of General Physician is Very Important in Chronic Phase of Disaster Medical Assistance

( Toshihiko Hata ),( Ken Ueda ),( Hiroshi Maruyama ),( Toshiki Takaya ),( Hiroshi Kuraishi ),( Kenji Maeda ),( Hiroki Tomita ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: In super acute phase after the Great East Japan Earthquake (11 March 2011), Disaster medical association team (DMAT) consisted of emergency physicians acted in disaster areas in the East Coast of Japan. On the other hand, these areas needed chronic care support by general physicians because of the enormous destructions of the medical facilities by the tsunami. So then, the Japanese Red Cross Society had sent disaster emergency outpatient assistance doctors to Isinomaki Red Cross Hospital, was intact because it was moved away from the Pacifi c Ocean three years ago. Methods: Determination of specialism by direct hearing and information from home page of each Red Cross Hospitals. Results: Cumulative 81 medical doctors were 38 internal medicine physicians, trainees 15, emergency physicians 12, surgeon 6, pediatrician 3, orthopedic surgeon 3, anesthesiologist 2, obstetrician 2, from April to August 2011. At fi rst 6 dispatched doctors were resident and did emergency outpatient work in Ishinomaki Red Cross Hospital for 6 days and dipatched. A lot of Cases of pneumonia and bronchial asthma due to debris and rubble by Tsunami were seen. All dispatch doctors corresponded to primary surgery including fractures and trauma, pediatric diseases, and posttraumatic stress disorder due to earthquake itself, tsunami damage, and radiation problem of Fukushima. Conclusions: The role of general physicians is very important in chronic phase of disaster medical assistance. Medical facilities in the area with many earthquakes should be moved apart from the sea in order to avoid the damage of tsunami.

Thermal analysis of carbon nanotube film irradiated by a pulsed laser

Toshiyuki Nakamiya,Tsuyoshi Ueda,Tomoaki Ikegami,Kenji Ebihara,Ryoichi Tsuda 한국물리학회 2008 Current Applied Physics Vol.8 No.3,4

The prepared multi-walled carbon nanotubes (MWCNTs) lm was mounted on the holder and the lm surface was ashed with asingle pulse of Nd:YAG laser (k = 532 nm) or a pulsed KrF excimer laser (k face damage was measured by inspecting the surface morphology. The dynamics of pulsed nanosecond laser heating process was sim-ulated by the solution of the one-dimensional heat conduction equation. The nite element method (FEM) was applied to solve theequation. The damage threshold energy density for MWCNTs lms ashed by a pulsed Nd:YAG laser were in the range 650 mJ/cm2and 790 mJ/cm2. At the laser uence of 790 mJ/cm2 with Nd:YAG laser, the surface reached the maximum temperature 913.C a t13 ns. Moreover, the Raman spectroscopy of MWCNTs lms before and after irradiation were measured. Graphitization occurredon the sample after irradiation over the threshold energy of surface damage.

Purification, characterization and gene cloning of metalloprotease from Nomuraea atypicola

Naomi Yamamoto,Mitsuhiro Ueda,Mizuho Kusuda,Masami Nakazwa,Kenji Ohuchi,Minoru Sakaguchi,Kuniyo Inouye,Kazutaka Miyatake 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

We have purified and characterized of metalloprotease metalloprotease from Nomuraea atypicola. N. atypicola was cultured in Sabouraud medium supplemented with powdered pupae. The metalloprotease from culture supernatant was purified to electrophoretically homogeneous state. The molecular mass of metalloprotease from N. atypicola was 50 kDa. The enzyme was most active at pH 8.5 and 40oC and stable at pH 5.0-7.0 and up to 40oC. The activity was inhibited by o-phenanthroline and EDTA. The N-terminal amino acid sequence of the enzyme showed a similarity to those of proteases (Metallo peptidase M36 family (Fungalysin)) from Coccidioides posadasii and Aspergillus fumigatus. The enzyme was found to be Fungalysin-like metalloprotease. cDNA encoding metalloprotease from N. atypicola was amplified by PCR using oligonucleotides deduced from the N-terminal endo peptide sequence, 5’- and 3’-RACE. Predicted enzyme structure consists of 637 amino acids with pro- and signal sequences. The mature enzyme had 391 amino acids and its deduced amino acid sequence coincided completely with the N- terminal amino end (20 amino acids) of metalloprotease purified from N. atypicola. We are studying on expression of the metalloprotease gene in Escherichia coli.

Unusual, broad red emission of novel Ce³⁺-activated Sr₃Sc₄O₉ phosphors under visible-light excitation

Hasegawa, Takuya,Kim, Sun Woog,Ueda, Tadaharu,Ishigaki, Tadashi,Uematsu, Kazuyoshi,Takaba, Hiromitsu,Toda, Kenji,Sato, Mineo The Royal Society of Chemistry 2017 Journal of Materials Chemistry C Vol.5 No.36

<▼1><P>A novel red emitting, Ce<SUP>3+</SUP>-activated Sr3Sc4O9 phosphor were discovered, and its luminescent properties were investigated.</P></▼1><▼2><P>Most conventional white light emitting diodes (white-LEDs) that are widely used as a new lighting system in next generation lights with Y3Al5O12:Ce<SUP>3+</SUP> (YAG:Ce<SUP>3+</SUP>)-based phosphors have a low colour rendering index (CRI) because the YAG:Ce<SUP>3+</SUP> phosphor shows a weak emission intensity in the red spectral region. Therefore, discovering a red-emitting phosphor with a high-efficiency is quite important to enhance the CRI of white-LEDs. In this study, we successfully discovered a novel, red-emitting Ce<SUP>3+</SUP>-activated Sr3Sc4O9 phosphor that can be excited by blue-light irradiation at 425 nm. A crystal structure of the host material was first determined by Rietveld refinement, which indicated that it should be isostructural with Ba3Ln4O9 (Ln = Sc, Y and Dy-Lu). As the Ce<SUP>3+</SUP> content increased, the X-ray diffraction patterns shifted to a lower angle, which suggested that the Ce<SUP>3+</SUP> ion could substitute Sc in the Sr3Sc4O9 host. Under UV and blue-light excitation, the Ce<SUP>3+</SUP>-activated Sr3Sc4O9 phosphor exhibited a broad emission band with a maximum peak at 620 nm, and its full width half maximum (FWHM) was 180 nm (4530 cm<SUP>−1</SUP>). The highest emission intensity was obtained for Sr3(Sc0.997Ce0.003)4O9, and the internal quantum efficiency of this phosphor under excitation at 425 nm was 53%. To the best of our knowledge, the emission band of the Ce<SUP>3+</SUP>-activated Sr3Sc4O9 phosphor is the longest wavelength for a reported Ce<SUP>3+</SUP>-activated oxide phosphor.</P></▼2>

Structural and Magnetic Properties of Antiferromagnetic Heusler Ru2MnGe Epitaxial Thin Films

Naoto Fukatani,Hirohito Fujita,Tetsuya Miyawaki,Kenji Ueda,Hidefumi Asano 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.3

Structural and magnetic properties were investigated for Heusler-type alloy Ru2MnGe thin films. Ru2MnGe films on MgO substrate were subjected to an in-plane compressive strain, and exhibitedenhanced antiferromagnetic (AFM) transition temperature (TN) up to 353 K, which exceeds by 37K from the cubic bulk material (TN = 316 K). We also observed the exchange coupling betweenRu2MnGe and Heusler-type ferromagnetic (FM) half-metal Fe2CrSi thin films. The present AFMHeusler alloy with relatively high TN is useful to fabricate high-quality all Heusler-type half-metalAFM/FM junctions and is a promising material for the emerging field of AFM spintronics.

Pathogenesis and Bone Resorption in Acquired Cholesteatoma: Current Knowledge and Future Prospectives

Mahmood A. Hamed,Seiichi Nakata,Ramadan H. Sayed,Hiromi Ueda,Badawy S. Badawy,Yoichi Nishimura,Takuro Kojima,Noboru Iwata,Ahmed R. Ahmed,Khalid Dahy,Naoki Kondo,Kenji Suzuki 대한이비인후과학회 2016 Clinical and Experimental Otorhinolaryngology Vol.9 No.4

Cholesteatoma is a cystic non tumorous lesion of the temporal bone that has the ability to destroy nearby structures by its power to cause bone resorption and as a result, fatal complications prevail. We aimed to conduct a comprehensive review for pathogenesis of acquired cholesteatoma, bone resorption mechanisms, and offer a future vision of this serious disease. We have reviewed different theories for pathogenesis of acquired cholesteatoma including the most relevant and updated ones with special emphasis on the mechanisms of bone resorption through Medline/PubMed research using the keywords ‘aetiopathogenesis, bone resorption, acquired cholesteatoma, temporal bone, and cytokines.’ In order to strengthen our study, we searched the reference lists of identified reviews. Cholesteatoma is a subject of debate among otolaryngologists since it was prescribed firstly. Over many decades, several theories were postulated for aetiopathogenesis of cholesteatoma with a tendency to follow more than one theory to explain the proper nature of that disease. Until now, the mechanism of bone resorption has yet to be more clarified. In the last century, a leap has occurred in the field of biomolecular cholesteatoma research which improved our knowledge about its pathophysiology and bone destructive mechanism. However, surgery is still the only available treatment. We conclude that discovery of new therapeutic choices for cholesteatoma other than surgery by the use of anti-growth, anti-proliferative, apoptotic agents as well as medications that antagonize osteoclastogenesis should be the main concern in the future clinical and experimental research work. Also, searching for predictors of the aggressiveness of cholesteatoma can affect the timing of intervention and prevent occurrence of complications.

Study of Trehalase during Autolysis of the Fruit-body in Pleurotus sp.

Alireza Arastoo,Masami Nakazawa,Tatsuji Sakamoto,Mizuho Kusuda,Hitoshi Kobayashi,Kenji Ouchi,Satoshi Inatomi,Mitsuhiro Ueda 한국버섯학회 2017 버섯 Vol.21 No.2

Fruiting bodies were degraded themselves by the several glycoside hydrolases after spore releasing from mature fruiting bodies or harvesting. The enzymes involved in autolysis such as glucanase and chitinase have been studied. However, there are almost no information about the relationship between several glycoside hydrolases and autolysis. In this study, we studied to obtain the enzymatic properties of trehalase, and also to get the new information on the relationship between trehalase and autolysis. Crude enzymes were prepared from each fruiting body of Pleurotus sp. (from the immature stage to the autolysis stage) and the trehalase activities were measured at each growth stage. Trehalase activities sharply increased in autolysis stage. Trehalase was partially purified from fruiting bodies of the autolysis stage using various column chromatography and its properties were examined. The optimum temperature was 50 °C and the optimum pH was 4.5. In order to elucidate the localization of trehalase, fruit bodies of the autolysis stage were divided into the stipes and the pileuses, and the each trehalase activity was measured. High trehalase activities were found in the pileuses. Furthermore, in order to elucidate trehalase activities in autolysis more detail, the each fruiting body of autolysis progressing stages was finely divided into the stipes and the pileuses, and their activities were measured. The activities in the outer part of the pileuses were highest at the initial stage of autolysis and the activities shifted from the outer side to the inner side of the pileuses according to the progress of autolysis.

Efficacy and safety of adding mizoribine to standard treatment in patients with immunoglobulin A nephropathy: A randomized controlled trial

( Keiji Hirai ),( Susumu Ookawara ),( Taisuke Kitano ),( Haruhisa Miyazawa ),( Kiyonori Ito ),( Yuichirou Ueda ),( Yoshio Kaku ),( Taro Hoshino ),( Honami Mori ),( Izumi Yoshida ),( Kenji Kubota ),( Y 대한신장학회 2017 Kidney Research and Clinical Practice Vol.36 No.2

Background: Mizoribine (MZR) is an immunosuppressive drug used in Japan for treating patients with lupus nephritis and nephrotic syndrome and has been also reportedly effective in patients with immunoglobulin A (IgA) nephropathy. However, to date, few randomized control studies of MZR are performed in patients with IgA nephropathy. Therefore, this prospective, open-label, randomized, controlled trial aimed to investigate the efficacy and safety of adding MZR to standard treatment in these patients, and was conducted between April 1, 2009, and March 31, 2016, as a multicenter study. Methods: Patients were randomly assigned (1:1) to receiving standard treatment plus MZR (MZR group) or standard treatment (control group). MZR was administered orally at a dose of 150 mg once daily for 12 months. Results: Primary outcomes were the percentage reduction in urinary protein excretion from baseline and the rate of patients with hematuria disappearance 36 months after study initiation. Secondary outcomes were the rate of patients with proteinuria disappearance, clinical remission rate, absolute changes in estimated glomerular filtration rate from baseline, and the change in daily dose of prednisolone. Forty-two patients were randomly assigned to MZR (n = 21) and control groups (n = 21). Nine patients in MZR group and 15 patients in the control group completed the study. No significant differences were observed between the two groups with respect to primary and secondary outcomes. Conclusion: The addition of MZR to standard treatment has no beneficial effect on reducing urinary protein excretion and hematuria when treating patients with IgA nephropathy.