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The role of mucosal barriers in human gut health
Kangseok Seo,Jeongmi Seo,Jiyoun Yeun,Haebin Choi,Young-In Kim,장선영 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.4
The intestinal mucosa is continuously exposedto a large number of commensal or pathogenic microbiotaand foreign food antigens. The intestinal epithelium formsa dynamic physicochemical barrier to maintain immunehomeostasis. To effi ciently absorb nutrients from food, theepithelium in the small intestine has thin, permeable layersspread over a vast surface area. Epithelial cells are renewedfrom the crypt toward the villi, accompanying epithelial celldeath and shedding, to control bacterial colonization. Tightjunction and adherens junction proteins provide epithelialcell–cell integrity. Microbial signals are recognized by epithelialcells via toll-like receptors. Environmental signalsfrom short-chain fatty acids derived from commensal microbiotametabolites, aryl hydrocarbon receptors, and hypoxiainducedfactors fortify gut barrier function. Here we summarizerecent fi ndings regarding various environmental factorsfor gut barrier function. Further, we discuss the role of gutbarriers in the pathogenesis of human intestinal disease andthe challenges of therapeutic strategies targeting gut barrierrestoration.
Elevated expression of exogenous RAD51 enhances the CRISPR/Cas9-mediated genome editing efficiency
Seo Jung Park,Seobin Yoon,Eui-Hwan Choi,Hana Hyeon,Kangseok Lee,김근필 생화학분자생물학회 2023 BMB Reports Vol.56 No.2
Genome editing using CRISPR-associated technology is widelyused to modify the genomes rapidly and efficiently on specificDNA double-strand breaks (DSBs) induced by Cas9 endonuclease. However, despite swift advance in Cas9 engineering,structural basis of Cas9-recognition and cleavage complex remainsunclear. Proper assembly of this complex correlates toeffective Cas9 activity, leading to high efficacy of genomeediting events. Here, we develop a CRISPR/Cas9-RAD51 plasmidconstitutively expressing RAD51, which can bind to singlestrandedDNA for DSB repair. We show that the efficiency ofCRISPR-mediated genome editing can be significantly improvedby expressing RAD51, responsible for DSB repair via homologousrecombination (HR), in both gene knock-out and knock-inprocesses. In cells with CRISPR/Cas9-RAD51 plasmid, expressionof the target genes (cohesin SMC3 and GAPDH) was reducedby more than 1.9-fold compared to the CRISPR/Cas9 plasmidfor knock-out of genes. Furthermore, CRISPR/Cas9-RAD51 enhancedthe knock-in efficiency of DsRed donor DNA. Thus, theCRISPR/Cas9-RAD51 system is useful for applications requiringprecise and efficient genome edits not accessible to HR-deficientcell genome editing and for developing CRISPR/Cas9-mediatedknockout technology.
Hwang, Kangseok,Kim, Mi Hyang,Seo, In-Soo,Oh, Chul Woong,Park, Jin-Ho The Korean Society of Fisheries and Aquatic Scienc 2014 Fisheries and Aquatic Sciences Vol.17 No.4
A pagurid hermit crab Catapaguroides fragilis was collected in Jeju Island, and described as new to the Korean fauna. It is the first member of the genus Catapaguroides known from Korea. Morphological descriptions of C. fragilis are presented with illustrations.
BLDC 고속 센서리스 구동의 ZCP 추정 오차 저감을 위한 Programmable Low Pass Filter 설계
서은정(Eunjeong Seo),이강석(Kangseok Lee),이우택(Wootaik Lee) 대한전기학회 2014 전기학회논문지 Vol.63 No.1
This paper presents a design method of programmable low pass filter(PLPF) which reduce an estimation error of a zero crossing point(ZCP) for a high speed brushless DC(BLDC) motor drive. BLDC motor sensorless drive is possible by estimation of ZCP. The ZCP estimated by detecting a change of back-EMF polarity has the estimation error because noises exist on the measured back-EMF. Therefore a calculated commutation timing using the ZCP is inaccurate. And the inexact commutation timing leads to ripples of 3-phase current and degradation of drive performance. This paper proposes the design method of the PLPF to overcome these problems. First, a speed calculated a inaccurate period of the ZCP is analyzed in the frequency domain. Then, the PLPF that has varying cut-off frequency according to change of the speed is designed on the frequency analysis result. The proposed method is verified by the experiment.
고속 BLDC 전동기 센서리스 구동에서 역기전력 신호의 노이즈와 샘플링 주기가 미치는 영향
서은정(Eunjeong Seo),이강석(Kangseok Lee),이우택(Wootaik Lee) 한국자동차공학회 2013 한국자동차공학회 부문종합 학술대회 Vol.2013 No.5
The purpose of this paper is to analyze effects of back-EMF(Electro Magnetic Force) signal noise and sampling period for Brushless DC(BLDC) motor sensorless drives at high speed. A typical zero crossing detection method detects zero crossing point using change of back-EMF polarity. Using this method, back-EMF signal noise and sampling period are the cause of performance degradation at high speed, because the number of back-EMF samples to detect zero crossing is small. Therefore the effects are analyzed by simulation on high performance BLDC motor drives at high speed.
Kim Taenam,Kim Kangseok,이기욱,Seo Minhye,황종국 한국화학공학회 2024 Korean Journal of Chemical Engineering Vol.41 No.1
The transportation sector is the largest contributor to carbon dioxide (CO 2 ) emissions, largely due to heavy-duty vehicles (HDVs) that inevitably rely on internal combustion engines. Electrifi cation is a promising technology for decarbonizing light-duty vehicles but it is diffi cult to apply to HDVs with long driving ranges and signifi cant weights. One possible solution is a mobile carbon capture (MCC) system that adapts onboard CO 2 capture and storage to the HDV. Although conceptual designs have been presented for MCC systems that use adsorbents and temperature-swing adsorption (TSA), the development of the adsorbent-based MCC remains in its infancy. Since adsorbents play a critical role in determining the overall weight, volume, and energy consumption of the MCC, the development of a high performance adsorbent is a key factor in the successful MCC design. In this review, we aim to provide guidelines for the design of CO 2 adsorbents for MCC in HDVs. First, we briefl y introduce the adsorbent selection criteria for TSA in MCC, including the CO 2 working capacity, purity, stability, and regeneration energy. Then, we summarize recent progress in the development of adsorbents tested under MCC-relevant conditions. Finally, the current challenges and future prospects of MCC are discussed.