고등학교 생물 I 교과서에 수록되어 있는 실험 . 관찰 방법의 적합성 조사

강미애,이천복 慶星大學校 1998 論文集 Vol.19 No.2

This study was performed to point some problems that teachers meet when they teach the experiments with high school biology I textbooks and also to suggest the solution. The results are as follow. Because of the lack of the detailed explanations on the 'method' (for example, the method of making the reagent and its concentration, the magnifying scale of the microscope), it is difficult for the teacher to guide the students to get the exact results. And the lack of diagrams, figures, and pictures with which the results are to be compared makes it difficult to judge the credibility of the result. Furthermore for a number of the experiment, it is not possible to get the results within 50 minutes. It is desirable to replace them with the plausible ones.

보릿짚의 수분함량 및 수확시기가 소각시 대기오염물질 발생에 미치는 영향

고지연,강항원,이재생,김춘송,박성태,김복진 한국환경농학회 2004 한국환경농학회지 Vol.23 No.2

영남지방 벼-보리 이모작답에서 가장 널리 시행되고 있는 보릿짚 처리방법인 소각이 대기환경에 미치는 영향을 소각시 발생하는 온난화가스, 대기오염가스 및 분진발생량을 중심으로 살펴보았다. 보릿짚 소각시 온난화가스 발생량은 CO₂376.8, CH₄ 1.56, N₂O 0.05 kg/10a 로서 CO₂의 발생량이 가장 많았으며, 대기 오염가스 중에는 유기물의 불완전 연소시 배출되는 CO발생량이 28.8 kg/10a로서 가장 많았다. 그 외 SO₂ H₂S, NH₃, NO등의 질소 및 황 함유 유해가스도 소량 발생되었고 발생된 PM 10 분진의 대부분은 인체에 미치는 영향이 PM 10보다 큰 PM 2.5로 이루어져 있었다. 또한 수확시기가 빠르거나 수분함량이 높은 보릿짚일수록 온난화가스 및 대기오염 가스의 발생량이 증가하고 소각 연기내 PM 10 분진중 PM 2.5의 미세분진 비중이 높았다. 수확적기로 알려진 출수 40일 이후의 보릿짚은 출수 후 40일된 보릿짚 소각시 발생하는 대기오염가스 발생량과 큰 차이가 없었다. This study was carried out to determine impacts of burning of barley straw produced from rice-barley double cropping paddy field on air quality by investigating emissions of greenhouse gases (CO₂, CH₄ and N₂O), air pollution gases (CO, SO₂, H₂S, NH₃ and NO) and particulate matters (PM 10 and PM 2.5). When the barley straw at a rate of 4.5 t/ha was burned at open status, the emitted GHGs amounts were CO₂ 376.8 kg/10a, CH₄ 1.56 and N₂O 0.06. The amount of CO emission was the largest among air pollution gases. These results showed that the range of 45~55% of total C in barley straw was emitted as CO₂-C, followed by CO-C (6.4~5.9%) and CH₄-C (0.5~0.7%). As far as moisture content in barley straw is concerned, the higher moisture content that the barley straw contains, the larger amount of air pollution gases and the higher portion of PM 2.5 in PM 10 were emitted when it burned. In case of harvesting time of barley straw, emission amounts of greenhouse, air pollution gases and PM 2.5 portion in PM 10 had tendency to increase when earlier harvested barley straw was burned.

흰쥐의 방사선 피폭후 생물학적 지표로서의 혈액효소

김진규,김상복,김국찬,천기정,김인규,박효국,이강석 대한방사선 방어학회 1993 방사선방어학회지 Vol.18 No.2

생물학적 선량평가를 위한 생화학적 지표 연구로서 흰쥐 혈액내 효소활성도의 변화를 조사한 결과는 다음과 같다. 1) Alkaline phosphatase 활성도는 0.1, 0.25, 0.5, 2, 4 Gy의 방사선 조사후 24시간까지 혈액내 활성도가 증가하였고 72시간 경과시에는 대조군과 비슷한 활성도를 보였다. Creatine kinase는 2, 4 Gy 방사선 조사후 혈액내에서 활성도에서 활성도가 72시간까지 증가하였으나 0.1, 0.25Gy, 0.5 Gy 방사선 조사시에는 커다란 변화를 보이지 않았다. 2) Malate dehydrogenase 활성도는 0.1, 0.25, 0.5 Gy 방사선 조사시에는 커다란 변화가 없었으며 lactate dehydrogenase는 방사선 조사후 활성도가 감소하였다. 3) GOT의 활성도는 선량률 0.1Gy/min.로 0.1, 0.25, 0.5, 2, 4 Gy 조사후에는 어떠한 변화도 없었으며 선량률 0.5 Gy/sec.로 0.5, 1, 1.5, 2, 3, 5, 7 Gy로 조사후에는 증가현상을 보이고 있다. Acid phosphatase 활성도는 상기의 어떠한 선량에서도 나타나지 않고 있다. 잠재적으로 이러한 효소들은 방사선 피폭의 지표물질로 사용될 수 있으며 생화학적 지식과 기술을 이용한 좀더 나은 지표물질을 찾기 위하여 계속적인 조사가 필요하다. Enzyme activity changes in rat blood as biochemical indicator useful for evaluating exposure dose were experimentally studied. The experimental results obtained are as follows: 1) Alkaline phosphatase activities increased in the blood serum until 24 hours after 0.1, 0.25, 0.5, 2 and 4 Gy irradiation and its activities returned mormal condition after 72 hours of post-irradiation. Creatine kinase activities increased in the blood serum until 72 hours after 2 and 4 Gy irradiation but any significant activity changes were not detected after 0.1, 0.25 Gy irradiation. 2) Malate dehydrogenase activities did not reveal available changes changes after 0.1, 0.25, 0.5 Gy irradiation and lactate dehydrogenase activities decreased in the blood serum after 0.1, 0.25, 0.5 Gy irradiation. 3) Glutamate oxaloacetate transaminase activity changes were detected in the blood serum after 0.1, 0.25, 0.5, 2, 4 Gy(0.1 Gy/min.) and GOT activities increased after 0.5, 1, 1.5, 2, 3, 5, 7 Gy(0.5 Gy/sec.). Any acid phosphatase activities were detected in the blood serum after 0.1, 0.25, 0.5, 2, 4 Gy(0.1 Gy/min.) and 0.5, 1, 1.5, 2, 3, 5, 7 Gy(0.5 Gy/sec.) irradiation. Potentially some of these enzymes can be used as indicator protein for radiation injury. Futher investigation is needed to find better biochemical indicatiors utilizing recent knowledge and techniques of biochemistry.

우라늄 투여후 간조직에서의 효소활성도의 변화

김국찬,이강석,김진규,김인규,박효국,천기정,김상복 대한방사선 방어학회 1993 방사선방어학회지 Vol.18 No.2

우라늄 피폭후 효소활성도가 내부피폭의 biomarker로서의 가능성을 연구하기 위하여 수서생물인 잉어9Caprinus carpio)의 복강내 우라늄 투여시 간조직에서 일어나는 여러가지 효소계의 변화를 조사하였다. 이러한 효소활성도의 변화는 우라늄 내부피폭의 biomarker로서 가능성을 가지고 있으며 연구결과는 다음과 같다. 1) 6일째까지 우라늄을 주사하여 적출한 간에서의 전체단백질량은 계속 감소하고 있다. 2) 세포내의 lysosome내에 함유하고 있는 acid protease와 β-glucuronidase의 활성도는 6일째 투여후까지 활성도가 감소하고 있다. 3) Alkaline phosphatase의 경우 6일째 우라늄 투여후까지 증가하고 있으며 반대로 acid phosphatase의 경우 6일째 우라늄 투여후까지 활성도가 급격히 증가하였고 glutamate oxaloacetate transaminase의 활성도는 완만하게 증가하고 있다. 4) Creatine kinase의 활성도는 완만한 감소를 보이고 있으며 malate dehydrogenase는 첫번 우라늄 투여후에 활성도가 급격히 감소하였고 3일째 우라늄 투여후에는 활성도가 거의 나타나지 않았다. We examined various enzyme activity changes by intraperitioneal injection uranium in the carp liver. These enzyme activity changes can be used as biochemical indicators of internal exposure to uranium. The results were followings ; 1) Total protein concentration decreased by intraperitoneal injection in the carp liver. 2) Lysosomal acid protease and β-glucronidase activities increased in the liver until sixth intraperitoneal injection of uranium, but lysosomal acid phosphatase activities decreased in the liver until the sixth injection of uranium. 3) Alkaline phosphatase activities sharply increased and Glutamate oxaloacetate Transaminase activities steadily decreased in the liver until the sixth injection of uranium. 4) Creatine %kinase activities steadily decreased and malate dehydrogenase activities sharply decreased in the liver after the primary injection of uranium. Any malate dehydrogenase activities was not detected after sixth injection of uranium.

Effect of Korean Ginseng on Teratogenecity of Chlorambucil on Developing Fetal Skeletogenesis in Rat

Chun, Yong Hyuck,Kim, Kang Ryune,Rha, Bok Young 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.2

發生中인 白鼠胎仔에서 Chlorambucil의 畸形原的 作用에 對한 人蔘의 效能 및 人蔘이 골격발생에 미치는 效果를 究明하기 위하여 初妊白鼠를 Chlorambucil 투여群, crude saponin 또는 panax ginseng 투여群, Chlorambucil과 人蔘 모두를 투여한 群 및 正常對照群으로 나누고 骨격發生이 活潑한 時期인 胎齡 第10日, 12日 및 14日에 上記物을 단독 또는 倂合투여한 後 分娩 하루前인 第20日에 胎仔를 모두 꺼내어 外形上所見, Alzarin Red S 染色所見 및 顯微鏡的 所見을 觀察하여 다음과 같은 結論을 얻었다. 1. 임신백서에 食餌性 panax ginseng의 투여는 胎仔의 成長, 體重 및 骨化에 영향을 주지 못하였으나 腹腔內 ginseng saponin의 투여는 骨化를 促進시켰고 그에 따라서 골격 및 體重의 增大를 招來하였다. 2. 임신백서에 對한 食餌性 panax ginseng, 또는 腹腔內 ginseng saponin 투여는 모두 發生中인 태자골격에 미치는 chlorambucil의 cytotoxity 또는 teratogenecity를 크게 抑制하였다.

The potential of mouse skin-derived precursors to differentiate into mesenchymal and neural lineages and their application to osteogenic induction in vivo.

Kang, Hyun Ki,Min, Seung-Ki,Jung, Sung Youn,Jung, Kyoungsuk,Jang, Da Hyun,Kim, O Bok,Chun, Gae-Sig,Lee, Zang Hee,Min, Byung-Moo D.A. Spandidos 2011 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.28 No.6

<P>Although previous studies indicate that skin-derived precursors (SKPs) are multipotent dermal precursors that share similarities with neural crest stem cells (NCSCs), a shared ability for multilineage differentiation toward neural crest lineages between SKPs and NCSCs has not been fully demonstrated. Here, we report the derivation of SKPs from adult mouse skin and their directed multilineage differentiation toward neural crest lineages. Under controlled in vitro conditions, mouse SKPs were propagated and directed toward peripheral nervous system lineages such as peripheral neurons and Schwann cells, and mesenchymal lineages, such as osteogenic, chondrogenic, adipogenic, and smooth muscle cells. To ask if SKPs could generate these same lineages in vivo, a mixture of SKP-derived mesenchymal stem cells and hydroxyapatite/tricalcium phosphate was transplanted into the rat calvarial defects. Over the ensuing 4 weeks, we observed formation of osteogenic structure in the calvarial defect without any evidence of teratomas. These findings demonstrate the multipotency of adult mouse SKPs to differentiate into neural crest lineages. In addition, SKP-derived mesenchymal stem cells represent an accessible, potentially autologous source of precursor cells for tissue-engineered bone repair.</P>

Effects of Liquid Pig Manure on Yield and Nutrition Supply of Green Manure Crops in Jeju

Kang, Ho-Jun,Yang, Sang-Ho,Kim, Yu-Kyoung,Jwa, Chang-Sook,Moon, Bong-Chun,Moon, Chae-Ran,Ko, Bok-Hee,Jung, Dae-Cheun 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.6

The effects of liquid pig manure (LPM) on the yield and nutrient supply of green manure crops, such as hairy vetch alone and hairy vetch + rye mixture, were investigated. We used three treatments: no-fertilizer (NF); LPM, $4.0t10a^{-1}$ ($N-P_2O_5-K_2O=4.0-0.024-4.0kg10a^{-1}$); and chemical fertilizer (CF; $N-P_2O_5-K_2O=4.0-0-4.0kg10a^{-1}$). The green manure crops, namely, Vicia villosa Roth. (hairy vetch) and Secale cereale L. (rye). were sown on March 11 and harvested on June 24, 2014. Yield of green manure crops significantly increased in hairy vetch + rye mixture plot compared with the hairy vetch alone plot. In hairy vetch alone plot, yield of green manure crops was increased by application of LPM and CF, but there were no significant differences. Yield of green manure crops in hairy vetch + rye fresh mixture plot significantly increased by the LPM and CF treatments, but no difference was noted between the LPM and CF treatments. Nitrogen supply by the green manure crops was higher in hairy vetch + rye mixture plot than hairy vetch alone plot. However, there was no significant difference. Phosphorus and potassium supply significantly increased in hairy vetch + rye mixture plot compared with hairy vetch alone plot. By treating LPM and CF, the nitrogen, phosphorus and potassium supply were higher than those in no fertilizer. In conclusion, LPM could replace CF. When planting green manure crops, the hairy vetch + rye mixture was more effective than hairy vetch alone to effectively use green manure crops.

Changes of Sesamin and Sesamolin Contents of Sesame Seeds during Grain Filling in Different Sowing Dates

Kang-Bo Shim,Jang-Hwan Park,Chung-Dong Hwang,Suk-Bok Pae,Jae-Chun Byun,Keum-Yong Park 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

To obtain some information on the change of antioxidant components of seeds during grain filling stage as affected by the sowing dates, lignan compounds were investigated according to days of flowering under different sowing dates. Sesamin and sesamolin contents showed significantly different by days of flowering and varieties. Both of sesamin and sesamolin content increased after flowering and reached highest at 40 days of flowering, but they started to decrease thereafter. Sesamin and sesamolin contents of sesame seeds changed with sowing dates. Generally, late sowing date of May 30 showed relatively higher accumulation rate of sesamin and sesamolin contents rather than other sowing dates, but overall patterns were a little different by varieties and lignan compounds. In case of variety Yangbaek, sesamin and sesamolin contents showed relatively higher at sowing date of May 30, but variety Yanghuck showed higher sesamin and sesamolin contents till 20 days of flowering when sowing date of May 30, but it showed to change that both lignan contents were relatively higher under sowing date of May 10.

The southeastern margin of the Cretaceous Yongdong Basin , Korea : a lacustrine fan - delta system

( Bok Chul Kim,Kang Min Yu,Hee Young Chun,Sung Ja Choi,Yoo Bong Kim ) 한국광물학회 1997 General Science Journal Vol.1 No.2

만성 편측 장골동맥 폐색 환자에서 대퇴동맥-대퇴동맥 우회로조성술 시 혈관성형술의 의의

강석형,천영덕,민연기,하헌균,전재영,김남렬,송태진,이재복,정석인,김윤환,최상용,황정웅 대한혈관외과학회 2002 Vascular Specialist International Vol.18 No.1

Purpose: The purpose of this article is to analysis the results of combined agioplasity and femorofemoral bypass in patients with unilateral iliac arterial occlusive disease. Method: During the 11-year period from 1990 to 2000, 44 patients with iliac artery occlusion and a hemodynamically significant contralateral iliac artery stenosis were treated by using a combination of percutaneous transluminal femoral bypass (n=14) at Korea university medical center. PTA was performed if the lesions in the donor iliac artery were less than 3 ??㎝ in length with in more than one well-localized lesion in either the common or external iliac artery, or both. Stent was deployed for suboptimal PTAs. The femorofermoral bypass was done within 3 to 5 days after PTA or stenting. Result: The mean age was 61.2 years. The Indications of fermorofermoral bypass were hypertension, ischemic heart disease, chronic obstructive lung disease, old age (>75), cancer, and previous abdominal operation, The complication rate of the combination treatment was lower than that of the others. Primary patency rate at 1 and 3 years were 89%, 78% for aortobifemoral bypass, 83%, 66% for iliofemoral bypass, and 77%, 65% for femorofemoral bypass, respectively. Conclusion: The combination of PTA with or without stent deployment and femorofemoral bypass can be a useful option for treating iliac occlusion and contralateral iliac stenosis in patients with severe comorbid illness, advanced age, and intra-abdominal pathology. Angioplasity can allow more widespread use of femorofemoral pypass in these patients.