호두 추출물의 항산화 활성과 신피질에서 세포 손상과 지질과산화 방지효과

배계선,황을철,권채화,김순희,최춘환,Bae Kae Sun,Hwang Eul Chul,Kwon Chae Hwa,Kim Soon Hee,Choi Chun Whan 한국생명과학회 2005 생명과학회지 Vol.15 No.1

본 연구에서는 호두박 분획층의 항산화 활성을 검색하기 위해 MeOH, $CH_2Cl_2$, EtOAc, BuOH 그리고 $H_2O$ 분획으로 추출하여 수행하였고, 각 분획층의 산화적 세포손상과 지질 과산화의 방지 효과를 in vitro에서 확인하였다. 그 결과 호두박의 free radical (DPP보 radical) 소거 활성은 EtOAc층에서 가장높게 나타났고, 활성산소종 억제율과 peroxynitrite $(ONOO^-)$ 소거 활성은 $CH_2Cl_2$층을 제외한 모든 분획층에서 높게 나타났다. 신피질 절편에 t-BHP의 처리 시 LDH의 방출과 지질과 산화를 증가시켰고 이러한 변화는 호두의 MeOH, EtOAc, n-BuOH 분획에 의해서 완전하게 방지되었다. 이러한 결과는 호두 추출물이 t-BHP에 의한 신장 세포 손상에 효과적이고 이러한 효과는 항산화력에 의한 것으로 추측된다. 또한 이러한 결과는 호두 추출물에서 많은 질병의 원인이 되고 있는 산화적 스트레스를 방지할 수 있는 효과적인 약물 개발의 가능성을 시사한다 To investigate the antioxidant activity of extract from the raw walnut, Juglans sinensis Dode, we prepared five fractions (methanol (MeOH), dichloromethane $(CH_2Cl_2)$, ethyl acetate (EtOAc), n-buthanol (n-BuOH) and dehydrogen monooxide $(H_2O)$ fractions) and examined. The effect of walnut extract on the oxidative stress was investigated in vitro. The DPPH (2,2-Di (4-tert-octylphenyl)-1-picrylhydrazyl) free radical scavenging activity of extract from raw walnut was shown in the following order: $EtOAc\;fraction<n-BuOH fraction< MeOH fraction<CH_2Cl_2\;fraction<H_2O$ layer. The result showed that the highest activity $(0.56{\mu}g/ml,\;IC_{50}.)$ was observed in EtOAc fraction, whereas n-BuOH fraction, MeOH fraction, $CH_2O_2$ fraction and $H_2O$ layer of $IC_{50}$ were $2.34{\mu}g//ml,\;3.88{\mu}g/ml,\;8.06{\mu}g/ml,\;and\;8.19{\mu}g/ml$, respectively. The radical scavenging activity assay of each fraction showed that the antioxidative activity was observed in the following order: EtOAc fraction $(74.27\pm1.56\%)>MeOH\;fraction\;(60.76\pm3.4\%)>n-BuOH\;fraction\;(59.32\pm0.88\%)>H_2O\;layer\;(41.69\pm2.06\%)$. These results revealed that all fractions, except for $CH_2Cl_2$ fraction, showed high antioxidative activity. Furthermore, the peroxynitrite $(ONOO^-)$ scavenging activity was assayed in each fraction. The result showed that the $ONOO^-$ scavenging activity of EtOAc fraction, MeOH fraction and n-BuOH fraction from raw walnut was $95.14\pm0.36\%,\; 90.02\pm1.19\%\;and\;89.41\pm0.81\%$, respectively. The tert-butylhydroperoxide (t-BHP) treatment in vitro increased lactate dehydrogenase release and lipid peroxidation in renal cortical slices. Such changes were completely prevented by addition of MeOH fraction, EtOAc fraction and n-BuOH fraction of walnut. These results indicate that the walnut extract exerts the benedicial effect against t-BHP-induced cell injury and its effect may be due to antioxidant action. In addition, it is suggested that walnut extract might be developed as the effective scavenger for the prevention of oxidative stress.

카드뮴의 처리(處理)가 상수(桑樹)의 생장(生長) 및 생체내(生體內) 대사(代謝)에 미치는 영향(影響)

배계선 ( Kae Sun Bae ),김영상 ( Yong Sang Kim ) 한국잠사학회 1983 한국잠사곤충학회지 Vol.25 No.1

카드뮴(Cd)의 葉面撒布가 뽕나무의 생장 및 生體內 代謝에 미치는 影響을 分析 檢討하여 얻어진 結果를 要約하면 다음과 같다. 1. 뽕잎의 被害症狀은 褐色斑點으로 나타났으며, 被害는 Cd 撒布 濃度가 높을수록 加增되었다. 2. 뽕나무의 條長과 條莖은 Cd의 濃度가 높을수록 生長이 抑劑되었는데, 특히 條長은 5%의 有意性이 認定되었으며 對照에 비해 5ppm 및 7ppm구는 각각 약 31% 生長이 抑制되었다. 3. 枝條 껍질의 Cd 含量은 잎의 그것보다 월등히 낮았다. 4. 뽕잎의 葉綠素, ATP含量과 呼吸量, GOT 및 GPT의 活性은 Cd 3ppm부터 顯著하게 떨어지기 시작했다. 5. 뽕나무의 生長과 物質代謝에 抑制影響을 초래하는 最低 處理濃度는 Cd 3ppm이었다. The study was carried out to examine effect of cadmium spraying on mulberry leaves on the growth and metabolism of mulberry tree. The results obtained were as follow: 1. Brown spot were appeared on the leaves as the symptoms of damage and the damage was remarkable with higher concentration of cadmium. 2. Higher concentration of Cd inhibited the length and diameter of mulberry branches with a significant difference (P<0.05) for the length. The growth of branch length in both 5 and 7ppm treatment of Cd was inhibited by 31% compared with the control. 3. Cadmium concentration of mulberry barks was noticeably lower than that of leaves. 4. Cadmium concentration over 3ppm inhibited significantly chlorophyll content, activitsies of respiration, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transase (GPT) and adenosine triphosphate (APT). 5. The lowest concentration of cadmium was identified to be 3ppm to incur the inhibitory effect on the growth and metabolism of mulberry and cadmium content of mulberry leaves at the concentration was 13.86ppm.

水耕재배 들깻잎의 수확량에 미치는 微量金屬 이온 効果

裵啓宣(Kae Sun Bae),成大東(Dae Dong Sung),鄭大守(Dae Soo Chung) 한국생명과학회 1998 생명과학회지 Vol.8 No.6

인공사료육잠(人工飼料育蠶)의 고온처리(高溫處理)에 관한 연구(硏究) -4령기(齡期)에 있어 고온(高溫)과 견형질(繭形質)과의 관계(關係)-

손흥대 ( Heung Dae Sohn ),배계선 ( Kae Sun Bae ) 東亞大學校附設 石堂傳統文化硏究院 1983 石堂論叢 Vol.7 No.-

繭形質 向上을 위한 高溫處理法을 究明하고자 4齡期의 人工飼料育蠶에 高溫處理(溫度, 經過, 日數 및 保護時間別)를 한 結果는 다음과 같다. 1. 溫度別 處理에서 全繭重과 繭層重은 37.5℃가 가장 무거웠으며 全繭重은 有意差가 認定되었다. 2. 經過日數別 處理에서의 全繭重과 繭層重은 1日째와 3日째에서 比較的 높은 增加를 보였다. 3. 保護時間別 處理에서 繭形質은 1日째, 16~20時間과 3日째, 8時間에서 현저하게 向上된 成績을 보였다. 踊體重의 增加率은 繭層重의 增加率에 비해 약 2배 程度이며 이로인해 各 增加區들은 繭層比率이 다소 낮았다. 4. 以上의 結果로 볼 때 4齡期 人工飼料育蠶의 繭形質 向上을 위한 合理的인 高溫處理法은 1日째, 37.5℃에서 16~20時間 處理인 것 같다. Effect of high temperature on improvement of cocoon character was carried out from the view point of temperature, progressive period and preserved time of 4th instar silkworm larva fed artificial diet. The results obtained were as follows; 1. Temperature: Cocoon weight was significantly heavier in the group treated at 37.5℃ compared with other groups, and cocoon shell weight was heavier in the group treated at 37.5℃ than other groups without significance. 2. Progressive period: Cocoon weight and cocoon shell weight were increased on 1st and 3rd day of 4th instar larva. 3. Preserved time: Cocoon character of 1st day larva preserved 16 to 20 hours at 37.5℃ and 3rd day larva preserved 8 hours at 37.5℃ were revealed remarkably good result. Increasing ratio of pupal body weight to cocoon shell weight was about two times, and owing to this result cocoon shell ratio was low. According to the results obtained it may be supposed that preserving 16 to 20 hours. at 37.5℃ on 1st day of 4th instar larva can improve cocoon character.

Effect on Apple pomace and Soybean pomace on cell growth of Bacillus stearothermophilus DL-3

Soon-Hee Kim(김순희),Kae-Sun Bae(배계선),Ji-Mo Kim(김지모),Hyung-Pil Seo(서형필),Ju-Sung Oh(오주성),Soon-Jae Jung(정순재),Byung-Ju Moon(문병주),Jin-Woo Lee(이진우) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.4

Effect of microbial product made of Bacillus stearothermohilus DL-3 on growth of pigs

Soon-Hee Kim(김순희),Kae-Sun Bae(배계선),Ju-Sung Oh(오주성),Soon-Jae Jung(정순재),Byung-Ju Moon(문병주),Jin-Woo Lee(이진우) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.10

대기공해(大氣公害)가 양잠(養蠶)에 미치는 영향(影響)에 관한 연구 -원잠종(原蠶種) 사육(飼育)에 있어서 아황산(亞黃酸) 가스 및 카드미움의 해(害)를 중심(中心)으로-

이종철 ( Chong Cheul Lee ),최진협 ( Jin Sub Choi ),배계선 ( Kae Sun Bae ),손흥대 ( Hung Dae Son ) 한국잠사학회 1979 한국잠사곤충학회지 Vol.21 No.1

대기공해가 잠아의 실용형질에 미치는 영향을 알고자 일본종계통 2품종과 중국종계통 2품종을 오염상 비오염상세, 척상별로 사육하고, 또 상엽 및 잠체중의 S, Cd함량을 분석한 결과는 다음과 같다. 1. 잠아경과일수는 일본종계통은 비오염구에 비해 오염구가 2.5일 정도 중국종계통은 4일정도 지연 및 불재일하였고 세척구는 중간 정도였다. 2. 잠아 5령극대체중, 견층중, 전견중 등은 비오염구, 세척구, 오염구 순으로 성적이 떨어졌다. 3. 화용비율은 상엽처리별로 볼 때 일본종은 유의성이 없었으나 중국종은 고도의 유의성을 나타냈다. 4. 오염상엽에 대한 저항성은 일본종계통에 비하여 중국종계통이 현저하게 약하며 동일계통에서도 종간에 차가 있다. 5. 오염지역의 상엽은 비오염지역의 상엽보다 S 및 Cd의 함량이 각각 약 30%, 300%증가하였다. 6. 잠체내에서도 비오염상 급여구는 S가 0.41%, Cd 0.013ppm이고 세척상 급여구는 S가 0.52%, Cd가 0.042ppm순으로 높았다. 7. S 및 Cd의 함량이 상엽에 가시적인 피해는 나타내지 않았으나 잠아에는 피해가 심했다. 8. 잠아의 실용형질은 공해로 인한 상엽질의 악화에서 입는 피해보다 상엽에 부착된 분전의 해가 더 컸다. 9. 이상을 종합해 보면 분장지대 선정에 있어서 중국종계통은 공해지역을 피할 것이며 부득이한 경우는 상엽을 세척급여하면 현저한 효과를 거둘 수 있을 것이다. Those studies were examined rearing two varieties on Japanese descent and two varieties of Chinese descent by feeding polluted mulberry leaves, non-polluted ones and water-cleaned ones respectively to find effects of air-pollution on the economic characters of silkworms and analysis of contents of Sulphur and Cadmium in the mulberry leaves and silkworms as followings; 1) Japanese descent of polluted part was delayed about 2.5 days than non-polluted part, Chinese descent was delayed about 4 days or inequal and water-cleaned part was medium in the silkworm larval duration. 2) Results of maximum weight of 5th instar, cocoon layer weight and cocoon weight were decreased in due order non-polluted, water-cleaned and polluted in the factors of mulberry. 3) Pupal ratio of Japanese descent was not shown statistical significance, but Chinese descent was revealed it obviously in the factors of mulberry leaves. 4) In the resistance of polluted mulberry leaves, Chinese descent was feeble obviously than Japanese and there were some difference even through among the same varieties. 5) The content of S and Cd of polluted area mulberry leaves was increased about 30% respectively than non-polluted area. 6) The fed part of non-polluted mulberry leaves was S 0.41% and Cd 0.013 ppm water-cleaned part was S 0.47% and Cd 0.024 ppm and polluted part was S 0.52% and Cd 0.042 ppm in the contents of S and Cd of silkworm larvae. 7) The contents of S and Cd didn`t make visible injury in mulberry leaves but made it seriously in silkworm larvae. 8) The injury of dust on mulberry leaves was more serious than that of quality of mulberry leaves by air-pollution in the economic characters of silkworm. 9) As above results, Chinese descent should avoid contaminated area and it rearing by water-cleaned mulberry leaves can get noticable results in inevitable case on selection of parents silkworm rearingzone.

人工酸性雨가 몇樹種의 葉綠素와 無機이온 含量에 미치는 影響

裵啓宣,朴定璂 동아대학교 환경문제연구소 1992 硏究報告 Vol.15 No.1

The Sulfuric acid and nitric acid mixture(3 to 1, V/V) was sprayed to trees three times a week(total 30 times) from July 1, 1991 in order to investigate the influence of artificial acid rain on the chlorophyll and inorganic ions of several trees. The results obtained were as follows : 1. Visible damages in leaves was recognized for Juniperus chinensis Var. Sargentii Henry and Cedrus deodara Loudon, however and the brown spots were appeared at pH 2.0 to 3.0 in broadleaf. 2. The contents of chlorophyll were decreased gradually with the lowering pH of artificial acid rain, especially decreased, more by 50 % for Cedrus deodara Loudon and by 30 % for Rosa multiflora thunb and Acer palmattum thunb at pH 2.0 compared with pH 3.0. 3. Lower pH of acid rain decreased greatly the inorganic ion contents, for instance, K^(+), Ca^(++) for Acer palmatum thunb and Mg^(++) for Ginkgo biloba at pH 2. 4. In conclusion, Juniperus chinensis Var. Sargentii Henry and Zelkova serrata Makino was identitied to be more resistant to acid rain since the decreasing rate of chlorophyll and inorganic ion contents is low.

Chlorella의 生長에 미치는 카드뮴 및 아연의 影響

裵啓宣,崔震浹,孫賢秀 東亞大學校 大學院 1984 大學院論文集 Vol.8 No.2

The study was carried out to examine effect of cadmium and zinc on the growth of Chlorella. The results obtained were as follows. 1. The chlorella cells damaged by heavy metals showed yellowish to milk-white symptoms at initial stage and died at later stage. 2. The cadmium has more inhibitory effect than on the turbidity cell number, chlorophyll contents and protein contents. 3. The chlorella cells were damaged more remarkably by the higher concentration of cadmium and zinc. 4. In conclusion, the minimum concentration of heavy metals to effect on the growth rate of chlorella cells were 2 ppm cadmium and 30 ppm of zinc.

夏伐後 桑葉의 光合成速度와 形態形成과의 관계

裵啓宣 東亞大學校 生命資源科學大學 附設 農業資源硏究所 1995 農業生命資援硏究 Vol.4 No.1

夏伐한 桑葉의 光合成速度와 形態形成과의 關係를 究明하기 위하여 圃場의 뽕나무 4品種을 선정하여 뽕잎의 生長에 따른 光合成측정과 기공과 잎의 두께 등을 측정하여 서로간의 상관관계를 조사한 결과를 종합하면 다음과 같다. 1. 光合成速度는 4品種 모두 開葉後 5일째(-)로부터 (+)로 바뀌고 40일째 최대치가 되었다. 품종별로 魯八>異桑 27號>八丈桑의 순이었다. 2. 呼吸速度는 開葉直後에 높고 그 후 급격히 감소하여 20日後부터 一定한 수치를 나타내었다. 品種別로는 노팔이 다른 3품종보다 높았으며 팔장상이 제일 낮은 편이었다. 3. 氣孔密度는 開葉初期에 낮고 25일후 최대치(950개/㎟ ∼1000개/㎟)에 달했다. 4. 4품종의 잎의 두께는 개엽직후에는 完成葉의 2/5 정도였던 것이 그 후 두꺼워졌다. 노팔이 30일째, 이상이 20일째, 국상 27호와 팔장상이 25일째에 최대값에 달했다. 品種에 따른 完成葉의 두께 차이는 10㎛이내였다. In order to investigate the relationship between photosynthetic rates and morphogenetic changes of mulberry, the experiment was carried out using 4varieties of mulberries grown in the field by measuring phtosynthetic rates, stomata and leaf thickness with the progress of leaf age. 1. Apparent photosynthetic rates after summer prunning changed from munus to plus value in 5days after unfolding in four varieties and reached the maximum in about 40days. Among these varieties, values in largeness was in the order of NOPAL>E-SANG>KUKSANG No.27> PALCHANGSANG. 2. Respiratory rates were high soon after unfolding reduced rapidly with the progress of leaf age and reached constand value in about three weeks. The value in NOPAL was higher than that in order three varieties and the value in PALCHANGSANG was lowest. 3. Stomatal distrubution was low soon after unfolding and reached the maximum(About 950 stomata/㎟ to 1000 stomata/㎟)in about 25days. 4. Leaf thickness in four varieties soon after unfolding was about two-fifths of that in mature leaves and became thick with the progress of leaf age. To reach maximum value took 30days in NOPAL, 20days in E-SANG 25days in KUKSANG No.27 PALCHANGSANG. The difference in leaf thickness of mature leaves were withing 10㎛.