다량의 붕소원소를 함유하는 식물의 중금속 이온 교환반응

배계선,성대동 한국환경과학회 1996 한국환경과학회지 Vol.5 No.6

The removal effectiveness of various heavy metal ions in boron-rich containing plants has been studied by means of spectroscopic and pH methods. Treatment of the boron-rich containing sample which was collected from cherry and root of cabbage to heavy metal ions is resulted in that an excellent removal effect shows in the case of large ionic size of heavy metal. Stability constants are depended on the variation of pH.

뽕나무 종자 발아시의 Protease 발현기구

裵啓宣 한국잠사학회 1993 한국잠사곤충학회지 Vol.35 No.1

The activity change of mulberry seeds protease was compared during germination for 5 days at 28°C in the dark place after daily hormone injection of different concentration. The protease from germinated mulberry seeds for 4 days was partially purified and the enzyme chacteristics was investigted. The protease activity of mulberry seeds treated by hormone was highest with 10 μM GA3 followed by 10 μM zeatin and 10 μM kinetin. The protease activity of mulberry seeds was increased by 14%, with 10 m/ agar culture than control at 4th day of germination. The protease from mulberry seeds was purified 313 fold by DEAE-Toyopearl 650 M, Butyl-Toyopearl, Hydrozylapatite and T'oyopearl HW 55 M. After purification, the specific activity of the enzyme was 175 units/mg. Optimum pH and temperature of protease from mulberry seeds was 5.0 and 37°C, respectively. The protease was stable below 37°C and the enzyme activity was decreased by 50%, when incubated at 52°C for 10 minutes. The protease activity of mulberry seeds was inhibited by metal ions such as mercury, iron, zinc, copper, but activited by magnesium, chromiunl, aluminium ions. The Km value of the protease was 0.89 mM with azocasein as a substrate.

人工酸性雨가 몇樹種의 葉綠素와 無機이온 含量에 미치는 影響

裵啓宣,朴定璂 동아대학교 환경문제연구소 1992 硏究報告 Vol.15 No.1

The Sulfuric acid and nitric acid mixture(3 to 1, V/V) was sprayed to trees three times a week(total 30 times) from July 1, 1991 in order to investigate the influence of artificial acid rain on the chlorophyll and inorganic ions of several trees. The results obtained were as follows : 1. Visible damages in leaves was recognized for Juniperus chinensis Var. Sargentii Henry and Cedrus deodara Loudon, however and the brown spots were appeared at pH 2.0 to 3.0 in broadleaf. 2. The contents of chlorophyll were decreased gradually with the lowering pH of artificial acid rain, especially decreased, more by 50 % for Cedrus deodara Loudon and by 30 % for Rosa multiflora thunb and Acer palmattum thunb at pH 2.0 compared with pH 3.0. 3. Lower pH of acid rain decreased greatly the inorganic ion contents, for instance, K^(+), Ca^(++) for Acer palmatum thunb and Mg^(++) for Ginkgo biloba at pH 2. 4. In conclusion, Juniperus chinensis Var. Sargentii Henry and Zelkova serrata Makino was identitied to be more resistant to acid rain since the decreasing rate of chlorophyll and inorganic ion contents is low.

호두 추출물의 항산화 활성과 신피질에서 세포 손상과 지질과산화 방지효과

배계선,황을철,권채화,김순희,최춘환,Bae Kae Sun,Hwang Eul Chul,Kwon Chae Hwa,Kim Soon Hee,Choi Chun Whan 한국생명과학회 2005 생명과학회지 Vol.15 No.1

본 연구에서는 호두박 분획층의 항산화 활성을 검색하기 위해 MeOH, $CH_2Cl_2$, EtOAc, BuOH 그리고 $H_2O$ 분획으로 추출하여 수행하였고, 각 분획층의 산화적 세포손상과 지질 과산화의 방지 효과를 in vitro에서 확인하였다. 그 결과 호두박의 free radical (DPP보 radical) 소거 활성은 EtOAc층에서 가장높게 나타났고, 활성산소종 억제율과 peroxynitrite $(ONOO^-)$ 소거 활성은 $CH_2Cl_2$층을 제외한 모든 분획층에서 높게 나타났다. 신피질 절편에 t-BHP의 처리 시 LDH의 방출과 지질과 산화를 증가시켰고 이러한 변화는 호두의 MeOH, EtOAc, n-BuOH 분획에 의해서 완전하게 방지되었다. 이러한 결과는 호두 추출물이 t-BHP에 의한 신장 세포 손상에 효과적이고 이러한 효과는 항산화력에 의한 것으로 추측된다. 또한 이러한 결과는 호두 추출물에서 많은 질병의 원인이 되고 있는 산화적 스트레스를 방지할 수 있는 효과적인 약물 개발의 가능성을 시사한다 To investigate the antioxidant activity of extract from the raw walnut, Juglans sinensis Dode, we prepared five fractions (methanol (MeOH), dichloromethane $(CH_2Cl_2)$, ethyl acetate (EtOAc), n-buthanol (n-BuOH) and dehydrogen monooxide $(H_2O)$ fractions) and examined. The effect of walnut extract on the oxidative stress was investigated in vitro. The DPPH (2,2-Di (4-tert-octylphenyl)-1-picrylhydrazyl) free radical scavenging activity of extract from raw walnut was shown in the following order: $EtOAc\;fraction<n-BuOH fraction< MeOH fraction<CH_2Cl_2\;fraction<H_2O$ layer. The result showed that the highest activity $(0.56{\mu}g/ml,\;IC_{50}.)$ was observed in EtOAc fraction, whereas n-BuOH fraction, MeOH fraction, $CH_2O_2$ fraction and $H_2O$ layer of $IC_{50}$ were $2.34{\mu}g//ml,\;3.88{\mu}g/ml,\;8.06{\mu}g/ml,\;and\;8.19{\mu}g/ml$, respectively. The radical scavenging activity assay of each fraction showed that the antioxidative activity was observed in the following order: EtOAc fraction $(74.27\pm1.56\%)>MeOH\;fraction\;(60.76\pm3.4\%)>n-BuOH\;fraction\;(59.32\pm0.88\%)>H_2O\;layer\;(41.69\pm2.06\%)$. These results revealed that all fractions, except for $CH_2Cl_2$ fraction, showed high antioxidative activity. Furthermore, the peroxynitrite $(ONOO^-)$ scavenging activity was assayed in each fraction. The result showed that the $ONOO^-$ scavenging activity of EtOAc fraction, MeOH fraction and n-BuOH fraction from raw walnut was $95.14\pm0.36\%,\; 90.02\pm1.19\%\;and\;89.41\pm0.81\%$, respectively. The tert-butylhydroperoxide (t-BHP) treatment in vitro increased lactate dehydrogenase release and lipid peroxidation in renal cortical slices. Such changes were completely prevented by addition of MeOH fraction, EtOAc fraction and n-BuOH fraction of walnut. These results indicate that the walnut extract exerts the benedicial effect against t-BHP-induced cell injury and its effect may be due to antioxidant action. In addition, it is suggested that walnut extract might be developed as the effective scavenger for the prevention of oxidative stress.

離農人口가 農村開發에 미치는 影響

裵啓宣 東亞大學校 學生生活指導硏究所 1981 學生硏究 Vol.10 No.-

뽕밭 災害의 問題點과 解決 方案

裵啓宣 한국잠사학회 1993 한국잠사곤충학회지 Vol.35 No.2

뽕나무종자 발아시의 Amylase활성에 미치는 호르몬의 영향

배계선 東亞大學校生命資源科學大學附設 農業資原硏究所 1996 農業生命資援硏究 Vol.5 No.1

뽕나무 종자(Morus Lhou Koidz)를 28℃의 암소에서 5일간 처리한 후 amylase 활성의 변화를 가각 비교조사 하였다. 그 결과는 다음과 같다. 1. 효소활성은 10ml 주입한 한천배지구가 대조구보다 4일째 약 14% 증가하였다. 2. Hormone 처리구의 발아종자는 효소활성이 GA₃가 가장 높았고 그 다음이 Zeatin, Kinetin과 Benzyladenine의 순이였다. 3. 결과적으로 자엽의 amylase활성에 대한 배축의 처리효과는 Kinetin과 Zeatin의 단독처리보다 GA₃의 혼합처리가 10% 더 효과적이라는 것이 암시되었다. The activity change of mulberry seeds amylase was compared during generation for 5 days at 28℃ in the dark place after daily hormone injection of different concentration. The results obtained are as follows; The amylase activity of mulberry seeds was increased by 14% with 10ml agar culture than control at 4th day of generation. The amylase activity of mulberry seeds treated by hormone was highest with GA₃10??M followed by Zeatin 10??M and Kinetin 10??M. The results suggest taht the regulative effect of the embryo axis on amylase activity in cotyledones is brought about more by 10% with the cooperative action of a gibberellin than with Zeantin and Kinetin alone.

桑實의 發芽와 뿌리의 生長에 미치는 카드뮴의 影響

裵啓宣,安始榮 동아대학교 환경문제연구소 1990 硏究報告 Vol.13 No.1

The experiment was conducted to investigate the influence of heavy metal Cadmium (Cd) on the germination and growth of roots in mulberry seeds by measuring protein pattern and Cadmium concentration of roots: The results obtained are as follows ; 1. Cadmium treatment had a tendency of decreasing the protein band of high molecular weight. 2. The higher Cd concentration inhibited the germination and the growth of mulberry roots. 3. Cd content of the root in 1 ppm, 5 ppm and 10ppm treatment increased 2 times, 4 times and 10 times compared with control group, respectively. 4. The lowest concentration of Cadmium was identified to be 5 ppm to incur the inhibition effect in the germination and growth of roots.

생활폐기물 처리에 관한 시민 의식조사에 대한 연구 : Food Waste 음식물 쓰레기를 중심으로

장성호,배계선 東亞大學校生命資源科學大學附設 農業資源硏究所 1998 農業生命資援硏究 Vol.7 No.1

A questionary survey was conducted to investigate citizen's attitude to the treatment of food wastes in Miryang city. In this study, collection efficiencies was 87.8%, as 281 individuals among 320 individuals. After volume-base charge system, 86.8% of answers perform source separation and 60.9% of respondents separate everything of recycle goods. The majority of respondents discharge food waste using standard envelope. The biggest problems for deposition of the food wastes are offensive odor and worm for reasons of sanitation. Almost citizens think that the period of deposition suit from two days to three days. More than 90.0% of the citizens recognize that compost product made from food wastes and recognition of the people for the composting and composting facilities was affirmative. Majority of the respondents thought that the administration and the provincial government need activity publicity for the source separation fixation of the food wastes.

夏伐後 桑葉의 光合成速度와 形態形成과의 관계

裵啓宣 東亞大學校 生命資源科學大學 附設 農業資源硏究所 1995 農業生命資援硏究 Vol.4 No.1

夏伐한 桑葉의 光合成速度와 形態形成과의 關係를 究明하기 위하여 圃場의 뽕나무 4品種을 선정하여 뽕잎의 生長에 따른 光合成측정과 기공과 잎의 두께 등을 측정하여 서로간의 상관관계를 조사한 결과를 종합하면 다음과 같다. 1. 光合成速度는 4品種 모두 開葉後 5일째(-)로부터 (+)로 바뀌고 40일째 최대치가 되었다. 품종별로 魯八>異桑 27號>八丈桑의 순이었다. 2. 呼吸速度는 開葉直後에 높고 그 후 급격히 감소하여 20日後부터 一定한 수치를 나타내었다. 品種別로는 노팔이 다른 3품종보다 높았으며 팔장상이 제일 낮은 편이었다. 3. 氣孔密度는 開葉初期에 낮고 25일후 최대치(950개/㎟ ∼1000개/㎟)에 달했다. 4. 4품종의 잎의 두께는 개엽직후에는 完成葉의 2/5 정도였던 것이 그 후 두꺼워졌다. 노팔이 30일째, 이상이 20일째, 국상 27호와 팔장상이 25일째에 최대값에 달했다. 品種에 따른 完成葉의 두께 차이는 10㎛이내였다. In order to investigate the relationship between photosynthetic rates and morphogenetic changes of mulberry, the experiment was carried out using 4varieties of mulberries grown in the field by measuring phtosynthetic rates, stomata and leaf thickness with the progress of leaf age. 1. Apparent photosynthetic rates after summer prunning changed from munus to plus value in 5days after unfolding in four varieties and reached the maximum in about 40days. Among these varieties, values in largeness was in the order of NOPAL>E-SANG>KUKSANG No.27> PALCHANGSANG. 2. Respiratory rates were high soon after unfolding reduced rapidly with the progress of leaf age and reached constand value in about three weeks. The value in NOPAL was higher than that in order three varieties and the value in PALCHANGSANG was lowest. 3. Stomatal distrubution was low soon after unfolding and reached the maximum(About 950 stomata/㎟ to 1000 stomata/㎟)in about 25days. 4. Leaf thickness in four varieties soon after unfolding was about two-fifths of that in mature leaves and became thick with the progress of leaf age. To reach maximum value took 30days in NOPAL, 20days in E-SANG 25days in KUKSANG No.27 PALCHANGSANG. The difference in leaf thickness of mature leaves were withing 10㎛.