유리피판을 이용한 하인두-식도 재건 치험례

봉정표,정윤규,이훈범,김주봉,이재화,나동균,김석원 大韓成形外科學會 1997 Archives of Plastic Surgery Vol.24 No.6

Hypopharyngo-esophageal cancer is usually found at late stage. Its main treatment is surgical intervention and it often requires radical resection. Reconstruction of the hypopharyngo-esophageal defect after the resection of carcinomas of the hypopharynx and the cervical esophagus has traditionally been carried out with deltopectoral or musculocutaneous flap. Another approach is to reconstruct the defect with the colon or stomach. Recent advances in microvascular surgical techniques have resulted in one stage reconstruction with free flap. Both jejunal free flap and radial forearm free flap is a good alternative for the reconstruction of hypopharyngo-esophageal defect. Six cases of the radial forearm flap and nine cases of the jejunal flap were used to reconstructed the hypopharyngo-esophageal defect in 15 patients. In jejunal free flap, upper margin was reconstructed with end to side anastomosis and lower margin with end to end anastomosis. In radial forearm free flap, we used trapezoid shaped flap and tubed by complete or partial tubing according to the condition of the defects. Stricture and fistula are troublesome complications. The complications were peritonitis(n=1) and fistula(n=1). We concluded that both forearm and jejunal free flap is a useful alternative in reconstruction of hypopharynx and cervical esophagus.

자기-광전이 형식을 유도하는 두 가지 다체사영연산자 방법의 비교

최상돈,이현정,석적영,조상규,강남룡 慶北大擧校 師範大學 科學敎育硏究所 2000 科學敎育硏究誌 Vol.24 No.-

We consider a magneto-optical transition theory based on the many-body projection technique. And we use two kinds of projectors ; projection-isolation operators and projection operators. The second method turns out to be more convenient than the first method.

혈행화된 두개골 피판을 이용한 안와저 및 안와하연의 재건술 치험례

정윤규,이훈범,김석원,봉정표,김균태,김주봉 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.5

A number of methods have been introduced for support the orbital floor following a maxillectomy without orbital exenteration or severe facial trauma. These methods including skin graft and muscular sling provided the unsatisfactory results, like as diplopia, orbital ptosis, enophthalmos and severe facial deformity. Therefore the bone and soft tissue reconstructions using microvascular free flaps were performed recently by many surgeons, but long time operation, donor site morbidity, postoperative large scar, and ptosis of the flap were pointed out as disadvantages of free flap reconstruction. Vascularized calvarial bone flap, a modified method of free calvarial bone graft, was adequate for reconstruction of the orbital floor and the infraorbital rim as a horizontal buttress, especially in case of poor vascular bed and postradiated state. The authors introduced the vascularized calvarial bone flap for the orbital floor and the infraorbital rim reconstruction in 3 cases of maxillectomy, and could be obtained satisfactory results aesthetically and functionally.

띠간 자기광학 전이에서 두 다체 사영방법의 비교

최상돈,김영미,이연주,석적영,조상규 慶北大擧校 師範大學 科學敎育硏究所 2000 科學敎育硏究誌 Vol.24 No.-

If we apply a circularly polarized light to semiconductors, in the external static magnetic field, then the electrons absorb the light at the resonance frequency, resulting in the interband-transition from valence band to conduction band. The linewidth is affected by various mechanisms. Here we will compare the isolated operator method and the single operator method.

Clonal Structures of Regionally Synchronous Gastric Adenomas and Carcinomas

Jung, Seung-Hyun,Kim, Shin Young,An, Chang Hyeok,Lee, Sung Hak,Jung, Eun Sun,Park, Hyeon-Chun,Kim, Min Sung,Chung, Yeun-Jun,Lee, Sug Hyung American Association for Cancer Research 2018 Clinical Cancer Research Vol.24 No.19

<P><B>Purpose:</B> Gastric adenoma (GA) is a premalignant lesion that precedes intestinal-type gastric carcinoma (GC). However, genetic progression mechanisms from GA to GC have not been clarified.</P><P><B>Experimental Design:</B> We performed whole-exome sequencing–based mutational analyses for 15 synchronous pairs of attached GAs and GCs.</P><P><B>Results:</B> There was no significant difference in the number of driver mutations or copy-number alterations between GAs and GCs. Well-known mutations of <I>TP53, APC, RNF43,</I> and <I>RPL22</I> were recurrently detected in synchronous GA/GC pairs. In addition, we discovered novel <I>KDM6A, PREX2, FAT1, KMT2C, GLI3,</I> and <I>RPL22</I> mutations and hypermutation in GAs, but did not identify recurrent drivers for GA-to-GC progression. Clonal structure analyses revealed that most GA/GC pairs exhibit parallel evolution with early divergence rather than stepwise evolution during GA-to-GC progression. Of note, three cases were identified as clonally nonrelated GA/GC pairs despite the lack of histologic differences. We found differences in dominant mutational signatures 1, 6, 15, and 17 in GA/GC trunks, GA branches, and GC branches. Compared with our previous work on synchronous colon adenoma/carcinoma genome structures, where most drivers were in the trunk with parallel evolution, synchronous GA/GC genomes showed a different model of parallel evolution, with many drivers in the branches.</P><P><B>Conclusions:</B> The preferred sequence of mutational events during GA-to-GC progression might be more context-dependent than colon adenoma progression. Our results show that nonclonal synchronous GA/GC is common and that GA genomes have already acquired distinct genomic alterations, suggesting caution in the diagnosis of synchronous GA and GC, especially in residual or recurrent cases. <I>Clin Cancer Res; 24(19); 4715–25. ©2018 AACR</I>.</P>

Investigation of the Prevalence of Legionella in Apartment Houses

Jung-Hee Lee,Myoung-Ki Park,Young-Su Kim,Bu-Geon Lim,Hye-Yeoun Lee,Young-Sug Kim 대한미생물학회 2021 Journal of Bacteriology and Virology Vol.51 No.2

Genomic structures of dysplastic nodule and concurrent hepatocellular carcinoma

Lee, Minho,Kim, Kyung,Kim, Shinn Young,Jung, Seung-Hyun,Yoon, Jonghwan,Kim, Min Sung,Park, Hyeon-Chun,Jung, Eun Sun,Chung, Yeun-Jun,Lee, Sug Hyung Elsevier 2018 Human pathology Vol.81 No.-

<P><B>Summary</B></P> <P>Although high-grade dysplastic nodule (HGDN) is a preneoplastic lesion that precedes hepatocellular carcinoma (HCC), the genomic structures of HGDN in conjunction with HCC remain elusive. The objective of this study was to identify genomic alterations of HGDN and its difference from HCC that may drive HGDN progression to HCC. We analyzed 16 regions of paired HGDN and HCC from 6 patients using whole-exome sequencing to find somatic mutation and copy number alteration (CNA) profiles of HGDN and HCC. The numbers of mutations, driver mutations, and CNAs of HGDNs were not significantly different from those of HCCs. We identified that the CNA gain of 1q25.3-1q42.13 was predominant in the HCCs compared with that in the HGDNs. Two cases (one nodule-in-nodule case and another case with closely attached HCC and HGDN) showed several overlapped driver mutations (<I>CTNNB1</I> and <I>CEBPA</I>) and CNAs (losses of <I>CDKN2A</I>, <I>RB1</I>, and <I>TP53</I>) between HGDNs and HCCs, suggesting their roles in the early HCC development. The other 4 cases with spatially separated HCCs and HGDNs showed few overlapped alterations between the paired HCCs and HGDNs. Mutations in <I>ERBB2</I> and <I>CCND1</I>, and CNAs (gains of <I>CTNNB1</I>, <I>MET</I>, and <I>SMO</I> and losses of <I>PTEN</I>, <I>TP53</I>, and <I>SETD2</I>) were identified as “HCC predominant,” suggesting their roles in the progression of HGDN to HCC. Our data show that HCCs are direct descendants of HGDNs in some cases, but there is no direct evidence of such relationship in spatially separated cases. Genomic features of HGDN identified in this study provide a useful resource for dissecting clues for the genetic diagnosis of HGDN and HCC.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Using WES, we identified genomic structures of HGDN in conjunction with HCC. </LI> <LI> Mutations of 2 genes and CNAs of 6 genes were identified as “HCC specific.” </LI> <LI> Copy number gain of 1q25.3-1q42.13 might play a crucial role in HGDN progression to HCC. </LI> </UL> </P>

Dietary factors related to hypertension risk in Korean adults-data from the Korean national health and nutrition examination survey Ⅲ

Jung-Sug Lee,Juyeon Park,Jeongseon Kim 한국영양학회 2011 Nutrition Research and Practice Vol.5 No.1

Regional differences between large cities and rural areas are observed in the Korean National Health and Nutrition Examination Survey (KNHANES). This present study was conducted to evaluate the effect of dietary factors on hypertension risk in Korean populations, especially residents of the Chungcheong province which was not in metropolitan area, using KNHANES Ⅲ. A total of 544 adults aged ≥ 19 years were placed into either the normotensive or the hypertensive group. Subject characteristics, BMI, blood pressure, and nutrient intakes were compared between the two groups using a chi-square test and t-test. We estimated odds ratios (ORs) using multiple logistic regression, adjusted for energy intake and selected covariates. There were significant differences in age, education level, alcohol consumption, and BMI between the normotensive and hypertensive groups. We found decreased ORs for the medium versus lowest tertile of calcium intake (multivariate OR = 0.43, 95% CI: 0.21-0.88), for the highest versus lowest tertile of calcium intake (multivariate OR = 0.43, 95% CI: 0.20-0.90) with significant trends in risk (P = 0.040), and for the medium versus lowest tertile of potassium intake (multivariate OR = 0.43, 95% CI: 0.20-0.89). Subjects with the highest sodium/calcium ratio had a 2.10-fold greater risk of hypertension compared to the subject with the lowest, with significant trends in risk (P = 0.002). Adequate calcium and potassium intake should be encouraged and regional differences should be considered in making a healthy plan for hypertension management.

Effects of Integrated Soil Amelioration Techniques to Mature Newly Established Research Fields

Jung, Sug-Jae,Hyun, Byung-Keun,Sonn, Yeon-Kyu,Cho, Hyun-Jun,Choi, Jung-Won,Lee, Pyeong-Ho,Lim, Dong-Hyuk 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.2

The Rural Development Administration moved to Jeonju for the balanced development of the land. This situation required establishment of new research fields with soils appropriate to cultivation. We applied a variety of amelioration techniques to mature soils of new research fields of the National Institute of Agricultural Science (NAS) and evaluated effects of the integrated amelioration techniques. The schedule of amelioration was following: 1) location of research fields was determined, 2) surface and subsoil samples were collected separately, 3) after aligning the top level of research fields, subsoil and surface soil were re-established with soil amendment, 4) the green manure crops were grown four seasons to improve the uniformity and increase the organic content of the research field, and 5) drainage canal and/or underdrainage were applied to poorly drained fields. The last green manure crop was rape in RDA fields and green barley in NAS fields. The average height, fresh weight, and dry matter weight of rape in good condition were 123 cm, $3,938kg\;10a^{-1}$, and $651kg\;10a^{-1}$, respectively. The height, fresh weight, and dry matter of green barley, on average, were 97 cm, $3,013kg\;10a^{-1}$, and $1,004kg\;10a^{-1}$, respectively. In the chemical properties of paddy field, pH and levels of silicate, calcium, magnesium, and potassium were in appropriate range but organic matter content of $16g\;kg^{-1}$ was less than the optimum level. In the chemical properties of upland field, pH and levels of phosphorus, calcium, magnesium, and potassium were appropriate range but organic matter content of $12g\;kg^{-1}$ was less than the optimum range. Evaluation of well-adapted soil was performed. The field in RDA was classified into the superior class with points ranging from 90 to 95 by the field evaluation test. The fields in NAS were mainly evaluated as the superior class with points greater than 85. However, some fields in NAS remained low quality with scores between 80 and 83. Further soil amelioration practices were suggested to fields with low soil quality.

<i>ERBB2</i> kinase domain mutation in a gastric cancer metastasis

LEE, JONG WOO,SOUNG, YOUNG HWA,KIM, SU YOUNG,PARK, WON SANG,NAM, SUK WOO,KIM, SANG HO,LEE, JUNG YOUNG,YOO, NAM JIN,LEE, SUG HYUNG Munksgaard International Publishers 2005 APMIS Vol.113 No.10

<P>ERBB2 is a member of the epidermal growth factor receptor (EGFR) family. Recent studies revealed that the kinase domain of the <I>ERBB2</I> gene was mutated in human cancers, including gastric cancer. Despite the importance of cancer metastasis in the pathogenesis of cancers, data on the <I>ERBB2</I> kinase domain mutation in cancer metastasis are lacking. In this study, to explore the possibility that <I>ERBB2</I> mutation is involved in the metastasis mechanism, we analyzed the kinase domain of <I>ERBB2</I> for the detection of somatic mutations in 58 gastric adenocarcinomas with lymph node metastasis. We found one <I>ERBB2</I> mutation, which was detected in the lymph node metastasis, but not in the primary tumor of the same patient. The <I>ERBB2</I> mutation was a missense mutation which substituted an amino acid in exon 21 (V832I). We simultaneously analyzed the somatic mutations of <I>EGFR</I>, <I>K-RAS</I>, <I>PIK3CA</I> and <I>BRAF</I> genes in the sample with the <I>ERBB2</I> mutation, and found that this metastatic carcinoma did not harbor any of the mutations. Our data suggest that <I>ERBB2</I> kinase domain mutation occasionally occurs in metastatic gastric carcinoma and might play a role in the metastatic process of some gastric carcinomas.</P>