발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

油壓 베인펌프의 內部 動的 壓力 特性에 關한 實驗的 硏究

鄭材鍊,李鍾純 全北大學校 1989 論文集 Vol.31 No.-

This paper presents an experimental study of the dynamic internal pressure within an oil hydraulic vane pump. The measurement of the dynamic internal pressures near the vane of a pressure balance type of an oil hydraulic vane pump with intravanes has been made to provide the essential information for the study of the pump dynamics and control, the pump design and the analysis of tribological problems in the sliding components. The influences of the discharge pressure and rotating speed of the vane on the dynamic pressure in four chambers surrounding a vane have been investigated. The results indicate that the surge pressures of the chambers at the instant of the start of discharge and closure are affected by the rotating speed, and the pressure in the intravane chamber maintains approximately constant values with remarkabel effect of pulsating discharge pressure. The profiles which have been presented in figures 4 through 7 indicate in detail the pressure distribution and acting forces of the vane to the camring.

Bovine botulism outbreak associated with incidental consumption of presumably contaminated leftover food

( Jae Won Byun ),( Kyung Hyun Lee ),( Ha Young Kim ),( Soon Seek Yoon ),( Ji Youl Jung1 ),( Woo Seog Jeong1 ),( Jae Ku Oem ),( Jong Soo Lim ),( Myoung Heon Lee ),( You Chan Bae ) 한국예방수의학회(구 한국수의공중보건학회) 2013 예방수의학회지 Vol.37 No.1

Nine cattle among 18 in a native Korean herd died or were euthanized within 3 days. The affected cattle showed cowshed. The pigs and dogs had been fed leftover food originating from nearby restaurants, and the cattle could presumably reach the contaminated food in the pig troughs. Necropsy revealed a few chicken bones and red pepper pieces as well as large amounts of grain and rice straw hay mixed in the rumen. Botulism type D was isolated in the ruminal contents of one of the cattle. We speculated that the outbreak was associated with the cattle incidentally eating presumably contaminated leftover food from the pig trough.

LCC 영향도 다이어그램 開發에 관한 硏究

정순성,이정재 東亞大學校 建設技術硏究所 1999 硏究論文集 Vol.23 No.1

The purpose of this study is to suggest Life Cycle Costing Influence Diagram, which can be used on behalf of a Sensitivity Analysis and Simulation that have used with many problems. As the results of case study, new risk analysis method, Life Cycle Costing Influence Diagram, introduced here is consist with results of a Sensitivity Analysis and Simulation. The three features of Life Cycle Costing Influence Diagram as new risk analysis method can be summarized as follows: (1) While analyzing LCC, a decision-maker can make out overall situations easily as to each aspect of steps. (2) It is convenient to identify the most effective variables in LCC (The lower the slope is, the more sensitive the effect as to LCC is) (3) It enables to evaluate LCC manually as well.

나노크기의 은 촉매제조 및 특성분석

정유성,이상량,신재순,김영철,박남국 한국공업화학회 2002 응용화학 Vol.6 No.2

Nano-sized silver catalysts have been prepared by alcohol and dioctyl esterof sodium sulphosuccinic acid, anionic surfactant, Aerosol OT(AOT). TOD/TPR andethylene oxide activity have been studied to investigate the characteristics of silvercatalysts. The sizes of Ag particles were estimated to be 10∼16 nm and the peak ofsilver oxide was not observed by XRD except for metallic silver. The desorptionpeak of molecular oxygen by TPD/TPR uniqely observed at about 400 ℃ in thecatalyst prepared by water-alcohol method. However, in the catalyst prepared bymicroemulsion method, the desorption peaks of molecular and atomic oxygens wereobserved at about 400 ℃ and 600 ℃, respectivly. The catalyst prepared by water-alcohol method showed higher activity ethylene oxide than the catalyst prepared by microemulsion method. It eems due to the larger silver particles and easier desorption of molecular oxygen from the catalyst prepared by water-alcohol method.

660kW급 풍력발전기 로터의 강제 응답 해석에 관한 연구

정순철,김덕수,이재응 중앙대학교 기술과학연구소 2001 기술과학연구소 논문집 Vol.31 No.-

본 연구에서는 660㎾급 풍력발전 시스템용 유도발전기 로터의 강제 응답 해석을 수행하였다. 이를 위하여 유한요소법을 이용한 회전체 시스템의 모드 해석을 수행하였으며, 모드 실험 결과 나타난 고유진동수와 모드 형상의 오차를 줄이기 위하여 유한요소 모델의 구조 변경을 수행하였다. 또한 회전체 시스템의 안정성 해석을 위하여 로터에 작용하는 여러 가지 외력(불평형력, 전자기력등)에 대한 강제 응답 해석을 수행하였다. 그 결과 공진주파수는 운전주파수인 30㎐(1800rpm)의 약 4.5배의 크기에서 발생하였으며, 운전주파수에서의 최대 처짐은 풍력발전기 회전체 시스템의 자중에 의한 정적 처짐과 공극에 비하면 매우 미소하여 거의 무시할 만 하다. 따라서 로터에 작용하는 외력은 시스템의 안정성 큰 영향을 미치지 않는 것이라 결론 지을 수 있다. In this paper, the stability analysis of the rotor system in the wind-power generator was performed by using Finite Element Method. Experimental modal analysis of generator rotor system was performed and the result were used to update the finite element model. The stability analysis of wind-power generator rotor-bearing system was performed through harmonic analysis with all possible external forces including the electromagnetic force, the unbalance force, the torsional force, etc. As a result of stability analysis, the first natural frequency of the bearing-rotor system is 4.5 times as large as operating frequency. The dynamic deflection at operating frequency is quite smaller than static deflection which is also quite smaller than the air-gap. So it can be concluded that the external forces of the rotor have no effect on stability of the rotor-bearing system.

InSb의 결정성장 및 특성

정양준,양순철,이형재 木浦大學校基礎科學硏究所 1989 基礎科學硏究誌 Vol.7 No.-

InSb crystals, both doped and undoped, were grown using the zone-refining technique in vacuum. The unintentionally doped crystals exhibit n-type conduction with the lowest carrier concentration of ??. The pure crystals show intrinsic characteristics even at very low temperatures. Hence very heavily doped n-type crystals were grown in an effort to determine the electron behaviors, not disturbed by the other bands over a wide temperature range. All the InSb crystals prepared in this work were self-seeded along the (111) direction, and the volatilization turned out to be very efficient in reducing the residual impurities during the growing process. The electrical conductivity and Hall coefficient were measured as a function of temperature from 90 to 570K. Theoretical calculations were carried out using a three-band model with the ?? and two hole bands taking into account scattering mehanisms. The results were consistently fitted to the experimental data with the adjustable parameters used in describing the temperature variation of band gap, heavy hole effective mass??, deformation potential??. The final fitting was obtained with the following values, ??, ??, ??. At high temperatures polar optical scattering is dominant for electron scattering and electron-hole scattering becomes very important.

례주(醴酒)의 제조와 품질특성

정순택,김선재 한국식생활문화학회 2001 韓國食生活文化學會誌 Vol.16 No.4

Li was a sweet beverage containing 2-3 percents ethyl alcohol made from malt by spontaneous fermentation from ancient custom to fifteenth century. Li was changed to the rice wine being a sweet beverage of low alcohol content using nuruk as starter and the sikhae which is non-alcoholic fermented beverage. Li was made for drinking and ceremony in Korea, China and Japan. It disappeared from the beverage items along with its method of manufacture from malt, but in Korean had made Li using nuruk until recent. We made Li according to Book of Imwon-Keongjae Ji (The book of country economy) methods for reappearance of Li. Fermentation characteristics for the production of Li with Saccharomyces cerevisiae, Saccharomyces bayanus and Saccharomyces sake were investigated. Among the yeast strains tested, Li fermented with S. sake showed higher alcohol production. Total sugar decreased considerably during the whole period of fermentation(30 hours), while ethyl alcohol content increased at 2.9∼83.52%. As the fermentation progressed, the pH decreased until the 30 hours of fermentation, while total acid increased during the same period. In fermentation of 36 hours, Li consisted of about 2.98∼3.52% of alcohol content, 5.3∼6.0% of total sugar, 1.45∼2.21㎎% of reducing sugar and total acidity were reached up to 24.4∼29.5㎎% for Li manufactured with S. cerevisiea sake, S. bayanus and S. sake.

세법상 특별감가상각의 효과에 관한 연구

권순철,정재을 경북대학교 경제경영연구소 1991 經商論集 Vol.19 No.4

Sn-3.5Ag 솔더 범프 플립칩의 무플럭스 열초음파 접합

洪淳珉,姜春植,鄭在弼 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.6

The thermosonic bonding was investigated as a fluxless solder bump flip chip bonding method. The 100 ㎛-diameter Sn-3.5 mass%Ag solder bumps were formed on test flip chip by laser ball bonding process. The test flip chips were bonded to a TSM-coated glass substrate using thermosonic bonding method at different temperature, bonding load and ultrasonic power. The die shear strength was evaluated and fracture surfaces were examined with SEM. The Sn-3.5Ag solder flip chip bonding was possible at lower temperature than the melting point of the solder. The die shear strength increased with increasing bonding temperature, bonding load, and ultrasonic power. However, at excessive bonding load condition over 1.0 N/bump, the die shear strength decreased due to the fracture at UBM/ Si-chip interface. The bump height decreased with increasing bonding load but did not change with ultrasonic power.