순비기나무 (Vitex rotundifolia L. f.) 삽수 종류 및 생장조정제 처리에 따른 발근율

Jeong Yeob Kim,Yoon Ki Hong,Song Hee Ahn,Jung Seob Moon,Eun Seok Park,Hee June Kim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.05

Background : This study was conducted to developed the propagation method by cutting for mass cultivation of Vitex roundifolia. We were pitched the cutting two times and treated plant growth regulators to enhance the rooting percentage. Vitex roundifolia is live in beach sandy soil south of Hwanghae-Do and Gangwon-Do. Vitex roundifolia have been used to bath foam. It is good for aromatic plant. It has 0.8% essential oil content in leaf and flower. Major components of essential oil were alpha-Pinene, Sabinene, beta-Pinen, 1,8-cineole, d-Limone. Despite the superior usability, it had not yet been made by the artificial cultivation Methods and Results : We were pitched the cutting of a first-year branch on June 5, which was greenwood cutting and July 17, which was semi-hardwood cutting at Kwangseung-ri beach, Gochanggun, Jeonbuk. The length of cutting was 10cm. It had 3~4 nodes, we stuck a cutting remain 2 nodes above ground on ordinary raise seedling soil. Rooting percentage was measured at 60 days after stuck a cutting. Rooting percentage was higher greenwoody cutting(95%) than semi-hardwood cutting(57.6%). In green wood cutting, there was no significance with plant growth, but chemical injury was occurred in IBA 5,000ppm. In semi-hardwood cutting, there was significance with plant growth regulators. The rooting percentages of all the treat were higher than control(no treatment). Rooting percentage was the highest in NAA 5,000ppm treated. Conclusion : Greenwood cutting method was more proper to propagation for Vitex roundifolia than semi-hardwood cutting. The optimum time to cutting for Vitex roundifolia propagation was the early in June. If miss a time to propagation Rooting percent was elevated by plant growth regulator.

Production of Phenyl Lactic Acid (PLA) by Lactic Acid Bacteria and its Antifungal Effect

Song, June-Seob,Jang, Joo-Yeon,Han, Chang-Hoon,Yoon, Min-Ho Korean Society of Soil Science and Fertilizer 2015 한국토양비료학회지 Vol.48 No.2

Phenyllactic acid (PLA) which is known as antimicrobial compound can be synthesized through the reduction of phenylpyruvic acid (PPA) by lactate dehydrogenase (LDH) of lactic acid bacteria (LAB). LAB producing PLA was isolated from Korea Kimchi and identified to Lactobacillus plantarum SJ21 by 16 rRNA gene sequence analysis. Cell-free supernatant (CFS) from L. plantarum SJ21 was assessed for both the capability to produce the antimicrobial compound PLA and the antifungal activity against four fungal pathogens (Rhizoctonia solani, Aspergillus oryzae, Botrytis cinerea, and Collectotricum aculatum). PLA concentration was investigated to be 3.23mM in CFS when L. plantarum SJ21 was grown in MRS broth containing 5mM PPA for 16 h. PLA production also could be promoted by the supplement of PPA and phenylalanine in MRS broth, but inhibited by the supplement of 4-hydroxyphenylpyruvic acid and tyrosine as precursors. Antifungal activity demonstrated that all fungal pathogens were sensitive to 5% CFS (v/v) of L. plantarum SJ21 with average growth inhibitions ranging from 27.32% to 69.05% (p<0.005), in which R. solani was the most sensitive to 69.05% and followed by B. cinerea, C. aculatum, and A. oryzae. The minimum inhibitory concentration (MIC) for commercial PLA was also investigated to show the same trend in the range from $0.35mg\;mL^{-1}$ (2.11 mM) to $0.7mg\;mL^{-1}$ (4.21 mM) at pH 4.0. The inhibition ability of CFS against the pathogens was not affected by heating or protease treatment. However, pH modification in CFS to 6.5 caused an extreme reduction in their antifungal activity. These results may indicate that antifungal activities in CFS were caused by acidic compounds like PLA or organic acids rather than proteins or peptides molecules.

Production of Phenyl Lactic Acid (PLA) by Lactic Acid Bacteria and its Antifungal Effect

June-Seob Song,Joo-Yeon Jang,Chang-Hoon Han,Min-Ho Yoon 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.2

Phenyllactic acid (PLA) which is known as antimicrobial compound can be synthesized through the reduction of phenylpyruvic acid (PPA) by lactate dehydrogenase (LDH) of lactic acid bacteria (LAB). LAB producing PLA was isolated from Korea Kimchi and identified to Lactobacillus plantarum SJ21 by 16 rRNA gene sequence analysis. Cell-free supernatant (CFS) from L. plantarum SJ21 was assessed for both the capability to produce the antimicrobial compound PLA and the antifungal activity against four fungal pathogens (Rhizoctonia solani, Aspergillus oryzae, Botrytis cinerea, and Collectotricum aculatum). PLA concentration was investigated to be 3.23mM in CFS when L. plantarum SJ21 was grown in MRS broth containing 5mM PPA for 16 h. PLA production also could be promoted by the supplement of PPA and phenylalanine in MRS broth, but inhibited by the supplement of 4-hydroxyphenylpyruvic acid and tyrosine as precursors. Antifungal activity demonstrated that all fungal pathogens were sensitive to 5% CFS (v/v) of L. plantarum SJ21 with average growth inhibitions ranging from 27.32% to 69.05% (p<0.005), in which R. solani was the most sensitive to 69.05% and followed by B. cinerea, C. aculatum, and A. oryzae. The minimum inhibitory concentration (MIC) for commercial PLA was also investigated to show the same trend in the range from 0.35 mg mL<SUP>-1</SUP> (2.11 mM) to 0.7 mg mL<SUP>-1</SUP> (4.21 mM) at pH 4.0. The inhibition ability of CFS against the pathogens was not affected by heating or protease treatment. However, pH modification in CFS to 6.5 caused an extreme reduction in their antifungal activity. These results may indicate that antifungal activities in CFS were caused by acidic compounds like PLA or organic acids rather than proteins or peptides molecules.

Antifungal Effect of 3-Phenyllactic Acid (PLA) produced by LacticAcid Bacteria and Characterization of Enzyme Catalyzing PLA Production

June-Seob Song,Joo-Yeon Jang,Heon-Hak Lee,Chan-Jung Lee,Min-Ho Yoon 한국토양비료학회 2015 한국토양비료학회 학술발표회 초록집 Vol.2015 No.5

Heavy Metal Resistant Phosphate Solubilizing Bacteria

Song, June-Seob,Walpola, Buddhi Charana,Chung, Doug-Young,Yoon, Min-Ho Korean Society of Soil Science and Fertilizer 2012 한국토양비료학회지 Vol.45 No.5

Soil samples collected from abounded mines of Boryeong area in South Korea were used in isolating bacterial strains and their capacity to solubilize inorganic phosphates and heavy metal tolerance were assessed in vitro. Three different inorganic phosphate sources (Ca phosphate, Fe phosphate, and Al phosphate) and four different heavy metals (Co, Cd, Pb and Zn) each with three concentrations ($100{\mu}g\;mL^{-1}$, $200{\mu}g\;mL^{-1}$, and $400{\mu}g\;mL^{-1}$) were used. The bacterial isolates PSB-1, PSB-2, PSB-3, and PSB-4 solubilized significantly higher amount of Ca phosphate during the first five days of incubation though subsequent drop in soluble phosphorus level in the medium was observed at the later stage (after 5 days) of the incubation. Solubilization of Ca phosphate and Fe phosphate was concomitant with the acidification of the culture medium compared to the control where it remained constant. Isolated strains could solubilize Fe phosphate to certain extent ($25-45{\mu}g\;mL^{-1}$) though solubilization of Al phosphate was found negligible. All the isolates were tolerant to heavy metals (Cd, Pb, and Zn) up to the concentration of $400{\mu}g\;mL^{-1}$ except PSB-1 and PSB-8, which were shown to be vulnerable to Co even at $100{\mu}g\;mL^{-1}$. Heavy metal tolerant strains should be further evaluated for plant growth promoting activities also under field conditions in order to assess their agricultural and environmental significance.

Heavy Metal Resistant Phosphate Solubilizing Bacteria

June-Seob Song,Buddhi Charana Walpola,Doug-Young Chung,Min-Ho Yoon 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.5

Soil samples collected from abounded mines of Boryeong area in South Korea were used in isolating bacterial strains and their capacity to solubilize inorganic phosphates and heavy metal tolerance were assessed in vitro. Three different inorganic phosphate sources (Ca phosphate, Fe phosphate, and Al phosphate) and four different heavy metals (Co, Cd, Pb and Zn) each with three concentrations (100 μg mL<SUP>-1</SUP>, 200 μg mL<SUP>-1</SUP>, and 400 μg mL<SUP>-1</SUP>) were used. The bacterial isolates PSB<SUP>-1</SUP>, PSB-2, PSB-3, and PSB-4 solubilized significantly higher amount of Ca phosphate during the first five days of incubation though subsequent drop in soluble phosphorus level in the medium was observed at the later stage (after 5 days) of the incubation. Solubilization of Ca phosphate and Fe phosphate was concomitant with the acidification of the culture medium compared to the control where it remained constant. Isolated strains could solubilize Fe phosphate to certain extent (25-45 μg mL<SUP>-1</SUP>) though solubilization of Al phosphate was found negligible. All the isolates were tolerant to heavy metals (Cd, Pb, and Zn) up to the concentration of 400 μg mL<SUP>-1</SUP> except PSB<SUP>-1</SUP> and PSB-8, which were shown to be vulnerable to Co even at 100 μg mL<SUP>-1</SUP>. Heavy metal tolerant strains should be further evaluated for plant growth promoting activities also under field conditions in order to assess their agricultural and environmental significance.

Long-term effects of antibiotic-coated Foley catheter on bacterial biofilm formations

( June Sung Son ),( Hyun Kyun Ki ),( Kyong Ran Peck ),( Won Sup Oh ),( Jae Hoon Song ),( So Youn Song ),( Sang Seob Lee ),( Ji Youl Lee ) 대한내과학회 2003 대한내과학회 추계학술대회 Vol.65 No.10

Stress Induced Phosphate Solubilization by Aspergillus awamori bxq33110 Isolated from Waste Mushroom Bed of Agaricus bisporus

Buddhi Charana Walpola,June-Seob Song,Kab-Yeul Jang,Min-Ho Yoon 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.3

A fungal strain, capable of solubilizing insoluble phosphate under diverse temperature, pH and salt conditions was isolated from Waste Mushroom bed of Agaricus bisporus in South Korea. Based on 18S rRNA analysis, the strain was identified as Aspergillus awamori bxq33110. The strain showed maximum phosphate solubilization in AYG medium (525 μg mL<SUP>-1</SUP>) followed by NBRIP medium (515 μg mL<SUP>-1</SUP>). The strain solubilized Ca₃(PO₄)₂ to a greater extent and rock phosphate and FePO₄ to a certain extent. However AlPO₄ solubilizing ability of the strain was found to be very low. Glucose at the rate of 2% (561μg mL<SUP>-1</SUP>) was found be the best carbon source for Aspergillus awamori bxq33110 to solubilize maximum amount of phosphate. However, no significant difference (P ≤0.05) in phosphorus solubilization was found between 1% and 2% glucose concentrations. (NH₄)₂SO₄ was the best nitrogen source for Aspergillus awamori bxq33110 followed by NH₄Cl and NH₄NO₃. At pH 7, temperature 30℃ and 5% salt concentration (674 μg mL<SUP>-1</SUP>) were found to be the optimal conditions for insoluble phosphate solubilization. However, strain Aspergillus awamori bxq33110 was shown to have the ability to solublize phosphate under different stress conditions at 30-40℃ temperature, pH 7<SUP>-1</SUP>0 and 0<SUP>-1</SUP>0% salt concentrations indicating it’ potential to be used as bio-inoculants in different environmental conditions.

Evaluation of Phosphate Solubilizing Potential of Three Burkholderia Species Isolated from Green House Soils

Buddhi Charana Walpola,June-Seob Song,Mi-Jung Keum,Min-Ho Yoon 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.4

Burkholderia anthina R-4183, Burkholderia diffusa R-15930 and Burkholderia stabilis LMG 14294 isolated from green house soils (Gongju-Gun area, South Korea) were characterized and their phosphate solubilizing ability was assessed. Under in vitro culture conditions, all three species were proved to be effective in solubilizing phosphates in varying degrees. Strain Burkholderia anthina exhibited the highest phosphate solubilization in NBRIP medium (665 ㎍ ㎖?¹) followed by Burkholderia diffusa (630 ㎍ ㎖?¹) and Burkholderia stabilis (578 ㎍ ㎖?¹). However, solubilization of FePO4 and AlPO4 was found to be poor in all the strains. Acidification by means of gluconic and oxalic acids accumulation in the culture medium could be the possible mechanism responsible for phosphate solubilization. Glucose at the rate of 3% was found be the best carbon source for Burkholderia anthina while other two Burkholderia species showed maximum phosphate solubilization at 2% of glucose. In the case of nitrogen sources, ammonium and nitrate were equally effective in solubilizing phosphates by Burkholderia species. Despite a slight decrease in phosphate solubilization observed at increasing temperature, all three Burkholderia species could withstand a temperature of 30-35℃, pH at the range of 7-9 and the presence of NaCl (up to 2.5%) without much compromising the phosphate solubilization. As shown with potted mung bean seedlings, all the three isolates could enhance soil fertility and plant growth indicating their great potential to be used as bio-inoculants.

Assessment of Plant Growth Promoting Activities of Phosphorus Solubilizing Bacteria

Buddhi Charana Walpola,June-Seob Song,Min-Ho Yoon 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.1

Plant growth promoting traits like production of indoleacetic acid (IAA), ammonia, hydrogen cyanide (HCN), siderophore, and like the enzyme activities of catalase, ACC deaminase, cellulase, chitinase and protease were assayed in vitro for twenty one phosphorus solubilizing bacteria isolated from soil isolates. Except SPP-5 and SPP-15 strains, all the other isolated strains produced IAA in various amounts of 10 to 23 ㎍ ml<SUP>-1</SUP>. All strains showed positive response for ammonia production and ACC deaminase activity implying that they are capable of growing in a N-free basal medium. Catalase activity was found to be superior in SPP-2, SPP-7, SPP-12 and SPP-17 compared to the other strains tested. HCN production was detected by 15 strains and among them SPP-9, SPP-15, SAph-11, and SAph-24 were found to be strong HCN producers. Except the isolates SPP-10, SPP-12, SPP-13 and SPP-14, all the other isolates produced more than 80% siderophore units. None of the strains showed cellulose and chitinase activity. SAph-8, SAPh-11, SAPh-24 and SPP-15 strains showed 35.84, 50.33, 56.64 and 34.78 U/ml protease activities, respectively. SPP-1, SPP-2, SPP-3, SPP-11, SPP-17, SPP-18, SAph-11 and SAph-24 strains showed positive response for all the tested plant growth promotion traits except cell wall degrading enzyme activities. According to the results, all the tested phosphorus solubilizing isolates could exhibit more than three or four plant growth promoting traits, which may promote plant growth directly or indirectly or synergistically. Therefore, these phosphorus solubilizing strains could be employed as bio-inoculants for agriculture soils.