Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang,Chul-Hak,Jun,Gyo,Hahm,Eun-Ryeong,Lee,Sangkyou,Park,Seyeon,Lee,Dug-Keun The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 uncleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

국내 여행상품 기획을 위한 리조트 사업의 핵심성과지표(KPI)에 대한 우선순위 및 가중치 개발

김학준(Hak Jun Kim) 한국관광연구학회 2011 관광연구저널 Vol.25 No.1

To survive the competitive environment in and outside the country, the Resort Industry needs to measure the past financial recordings and future values. The Resort Industry being an Intangible Service Industry requires measurement in intangible values. By these measurements the limited capitals should be selected and invested to differentiate itself from competitors and secure competitiveness. Currently, there are items for evaluating the present, future, and past of the Resort Industry, however no academically established priority of the items. The purpose of this research, a follow-up research of Kim, Hak-Jun & Kim, Dea-Kwan(2007), “A study of suitability of the indicators for the Key Management Performance Evaluation in the resort industry”, is development on significancy and priority of Resort Industry KPI for domestic travel product planning. As a result of the study, Customer Perspective was understood to be most important, next Financial Perspective, Internal Process, and lastly the Learning and Growth Perspective. KPI`s priority consists of Operating Profit Rate in first place with a significant rate of 0.147. Next, following up was the Revisiting Intention Rate and Customer Satisfaction Rate. The results implicate that in the domestic Resort Industry the business strategy should select and invest in limited capitals by investing in the Customer Perspective and corresponding to the Needs and Wants of the customer to induce revisits.

여행상품 기획을 위한 서울시내 특1급 호텔의 핵심성과지표(KPI)에 대한 우선순위 및 가중치 개발

김학준(Hak Jun Kim) 한국관광연구학회 2011 관광연구저널 Vol.25 No.2

The Hotel Business being an Intangible Service Industry requires measurement in intangible values. By these measurements the limited capitals should be selected and invested to differentiate itself from competitors and secure competitiveness. Currently, there are items for evaluating the present, future, and past of the Hotel Business, however no academically established priority of the items. The purpose of this research, a follow-up research of Kim, Dea-Kwan, Kim, Hak-Jun & Park, Yang-Woo(2009), A Study on the Development of KPI in the Super Deluxe Hotel Business in Seoul Korea Using BSC, is development on significancy and priority of KPI in the Super Deluxe Hotel Business in Seoul Korea for travel product planning. As a result of the study, Financial Perspective was understood to be most important, next Customer Perspective, Internal Process, and lastly the Learning and Growth Perspective. KPI`s priority consists of customer satisfaction measurement in first place with a significant rate of 0.145. Next, following up was operating profit to sales ratio of sales and achievement ratio of operating profit. The results implicate that in the Super Deluxe Hotel Business in Seoul Korea the business strategy should select and invest in limited capitals by investing in the increased customer satisfaction measurement and corresponding to the Needs and Wants of the customer to increase customer satisfaction measurement.

AHP를 활용한 국내 위탁급식사업의 KPI 우선순위 및 가중치개발에 관한 연구

김학준(Hak Jun Kim),김홍길(Hong Kil Kim),김대관(Dae Kwan Kim) 한국관광연구학회 2011 관광연구저널 Vol.25 No.4

The contracted foodservice business as an intangible service industry requires measurement in intangible values. By these measurements the limited capitals should be selected and invested to differentiate itself from competitors and secure competitiveness. Currently, there are items to evaluate the present, future, and past of the Contracted Foodservice Business, however no academically established priority of the items. The purpose of this research, a follow-up research of Kim, Hak-Jun & Kim, Ju-Yeon(2009) is development on significancy and priority of KPI in Korea. As a result of the study, customer perspective was understood to be most important, next financial perspective, internal process, and lastly the learning and growth perspective. KPI`s priority consists of customer satisfaction measurement in first place with a significant rate of 0.187. Next, following up was achieved profit targets and achieved sales goals. The results implicate that in the contracted foodservice business in Korea the business strategy should select and invest in limited capitals by investing in the increased customer satisfaction measurement and corresponding to the needs and wants of the customer to increase customer satisfaction measurement.

비구조적 퍼지 의사결정지원시스템을 활용한 건설재해 예방대책에 관한 연구

이용준,정성춘,이학기 東亞大學校 建設技術硏究所 2004 硏究論文集 Vol.27 No.2

The hazard factors in building construction project are various and their bad effects with regard to project's efficiency are increasing due to its enlargement and complexity. However. the assessment of the construction safety is commonly dependent upon the statistical and historical information. since the existing systematic tool assisting to the effective decision making for estimating the safety prior to construction phase is inaccurate. In addition. it is true that there are the various difficulties to analyze and diagnosis the construction safety with the probabilistic approach. because there are the many vague factors and limited resources. Therefore. the purpose of this research is to provide the way to establish the efficient preventable countermeasure against the hazard factors. corresponding to the category of the hazard characteristic. by using non-structural fuzzy decision support system for diagnosing effectively the construction project safety.

지방족 탄화수소의 할로겐 유도체 수용액의 광촉매-광분해

전진,정학진,김해진,김삼혁 한국환경과학회 1997 한국환경과학회지 Vol.6 No.1

The rates of photodegradation, reactivities, and mechanisms of photooxidation for the aqueous solution containing with halogen derivatives of aliphatic hydrocarbons have been discussed with respect to the kinds of photocatalysts, concentration of photocatalytic suspensions, strength of radiant power , time of illumination, changes of pH of substrate solution, wavelength of radiation, and pressure of oxygen gas saturated in the solution. These aqueous solutions suspended with 0.5 gL^-1 TiO_2 powder have been photodecomposed in the range of 100 and 93.8% per 1 hour if it is illuminated with wavelength (λ≥ 300nm) produced from Xe-lamp(450W). The photocatalytic abilities have been increased in the order of Fe_2O_3 < CdS < CeO_2 < Y_O2_3 < TiO_2, and rates of photodegradation for the solution have maximum values in the condition of pH 6∼8 and 3 psi-O_2 gL^-1. These rates for the photooxidation per 1 hour were dependent on the size of molecular weight and chemical bonding for organic halogen compounds and the rates of photodegradation were increased in the order of C_2H_5Br < CH_2Br_2 < C_5H_11Cl C_2H_4Cl_2 < trans-C_2H_2Cl_2 < cis-C_2H_2Cl_2 The t_1/2 and t_99% for these solutions were 5∼21 and 40∼90 minutes, respectively, and these values were coincided with initial reaction kinetics(r_0). It was found that reaction of photodegradation has the pseudo first-order kinetics controlled by the amount of h^+_VB diffused from a surface of photocatalysts.

20주간의 혼합운동프로그램이 정신지체 성인의 신체조성과 체력에 미치는 효과

전종귀,염동삼,조병준,이상기,박희근,한동수,장학영 한국특수체육학회 2004 한국특수체육학회지 Vol.12 No.3

The purpose of this study was to figure out the effects of participation in a combined exercise program on the body composition and physical fitness of adults with mental retardation. Fifteen adults with mental retardation in a special institute took part in aerobic and strength , combined exercise at 55 - 69%HRmax at least one hour a day, 3 times a week for 20 weeks. The body composition and physical fitness profiles of subjects were measured before and after training period, respectively. Dependent t-test was used to analyze the statistical significance. The results were as follows; First, participants showed more favorable % body fat, lean body mass, fat mass, WHR after training. Second, participants showed more improved upper body strength and balance after training. The findings imply that the combined exercise program for long duration had significant effects on the health promotion of adults with mental retardation.

地方自治制度의 改革에 관한 硏究 : 英國保守黨 Thatcher 政府를 중심으로

朴準鶴 동국대학교 지역개발대학원 1996 開發論叢 Vol.5 No.-