Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model

Ming Zhang,Xing Fan,Bing Fang,Chengzhen Zhu,Jun Zhu,Fazheng Ren 한국미생물학회 2015 The journal of microbiology Vol.53 No.6

Probiotics have been suggested as a prophylactic measure in colon cancer. The aim of this study was to investigate the impact of Lactobacillus salivarius Ren (Ren) in modulating colonic microbiota structure and colon cancer incidence in a rat model after injection with 1,2-dimethyl hydrazine (DMH). The results indicated that oral administration of Ren could effectively suppress DMH-induced colonic carcinogenesis. A significant decrease in cancer incidence (87.5% to 25%) was detected in rats fed with a dose of 5 × 1010 CFU/kg bodyweight per day. Using denaturing gradient gel electrophoresis and Real-time PCR combined with multivariate statistical methods, we demonstrated that injection with DMH significantly altered the rat gut microbiota, while Ren counteracted these DMH-induced adverse effects and promoted reversion of the gut microbiota close to the healthy state. Tvalue biplots followed by band sequencing identified 21 bacterial strains as critical variables affected by DMH and Ren. Injection of DMH significantly increased the amount of Ruminococcus species (sp.) and Clostridiales bacteria, as well as decreasing the Prevotella sp. Administration of Ren reduced the amount of Ruminococcus sp., Clostridiales bacteria, and Bacteroides dorei, and increased the amount of Prevotella. Real-time PCR results were consistent with the results derived by t-value biplots. These findings suggested that Ren is a potential agent for colon cancer prevention. In conclusion, the results in the present study suggest a potential therapeutic approach based on the modulation of intestinal microflora by probiotics may be beneficial in the prevention of colorectal carcinogenesis.

中文字辭重出之組合釋例

王仁鈞 단국대학교 중어중문학과 1980 中國文學報 Vol.- No.4

Research progress and treatment of radiation enteritis and gut microbiota

Huiwen Ren(Huiwen Ren),Qi Wu(Qi Wu),Zhiqiang Sun(Zhiqiang Sun),Mingming Fang(Mingming Fang),Jun Liu(Jun Liu),Judong Luo(Judong Luo) 대한방사선종양학회 2023 Radiation Oncology Journal Vol.41 No.2

Radiation enteritis is a kind of intestinal radiation injury in patients with pelvic and retroperitoneal malignancies after radiotherapy, and its occurrence and development process are very complicated. At present, studies have confirmed that intestinal microecological imbalance is an important factor in the formation of this disease. Abdominal radiation causes changes in the composition of the flora and a decrease in its diversity, which is mainly manifested by a decrease in beneficial bacterial species such as Lactobacilli and Bifidobacteria. Intestinal dysbacteriosis aggravates radiation enteritis, weakens the function of the intestinal epithelial barrier, and promotes the expression of inflammatory factors, thereby aggravating the occurrence of enteritis. Given the role of the microbiome in radiation enteritis, we suggest that the gut microbiota may be a potential biomarker for the disease. Treatment methods such as probiotics, antibiotics, and fecal microbiota transplantation are ways to correct the microbiota and may be an effective way to prevent and treat radiation enteritis. Based on a review of the relevant literature, this paper reviews the mechanism and treatment of intestinal microbes in radiation enteritis.

Efficacy and safety of olmesartan medoxomil/amlodipine fixed-dose combination for hypertensive patients uncontrolled with monotherapy

Jun-Ren Zhu,Shu-Yang Zhang,Ping-Jin Gao 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.12

This study aimed to evaluate the efficacy andsafety of olmesartan medoxomil (OM)/amlodipine (AML)20/5 mg fixed-dose combination tablet in Chinese mild tomoderately hypertensive patients with inadequate bloodpressure (BP) control on monotherapy. Two multicenter,randomized, double-blind, double-dummy, active-controlled,parallel group clinical trials were conducted. Afterscreening and a 2-week placebo run-in period, patients with95 mmHg B seated diastolic blood pressure (SeDBP) \110 mmHg received monotherapy with OM 20 mg (inStudy 1) or AML 5 mg (in Study 2), once daily for4 weeks. Patients with 90 mmHg B mean SeDBP \110 mmHg at the end of the monotherapy period wererandomized to receive OM/AML 20/5 mg treatment orcontinue with the monotherapy, once daily for 8 weeks. OM/AML (20/5 mg) treatment significantly lowered bothsystolic and diastolic BP at 4 and 8 weeks compared to40 mg olmesartan or 5 mg AML. The incidence of drugrelatedadverse effects did not differ significantly betweenthe groups. OM/AML 20/5 mg was superior to OM 40 mgor AML 5 mg monotherapy in lowering BP in Chinesemild to moderately hypertensive patients with inadequateBP control on monotherapy. No new or unexpected safetyissues were identified with OM/AML combination therapycompared to monotherapy.

The Empirically Comparative Analysis of Benchmarking Studies in China and Abroad

Jun-yi Ren 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

To make statistical classifying of the numerous publications on benchmarking studies in China and abroad, to reveal the conditions and trends of benchmarking study both in china and abroad to make empirically comparative analysis between them at the four aspects like number of publications, the research time dimension, mainstream of research, development conditions and development trend, and then to summarize some provlems that exist in Chinese benchmarking research areas.

Activation of formyl CH and hydroxyl OH bonds in HMF by the CuO(1 1 1) and Co₃O₄(1 1 0) surfaces: A DFT study

Ren, Jun,Song, Kai-he,Li, Zhenhuan,Wang, Qiang,Li, Jun,Wang, Yingxiong,Li, Debao,Kim, Chan Kyung Elsevier 2018 APPLIED SURFACE SCIENCE - Vol.456 No.-

<P><B>Abstract</B></P> <P>The first principle calculations with on-site Coulomb repulsion U terms were carried out to investigate the 5-hydroxymethylfurfural (HMF) adsorption on the CuO(1 1 1) and Co<SUB>3</SUB>O<SUB>4</SUB>(1 1 0) surfaces, two widely used oxidation catalysts. The adsorption of HMF molecule is energetically favoured in both cases, and HMF is more inclined to bridge adsorption via hydroxyl and formyl groups binding with surface O and metal sites. Moreover, the adsorption energy relies on both the coordination type of surface lattice oxygen to which the H atom binds and the formation of H-bond involving hydroxyl and formyl groups on the adsorbed HMF. Also, the hydroxyl OH bond breaking is very easy and is likely to be the first step in HMF oxidation, and then the OH insertion reaction to produce 2,5-furandicarboxylic acid (FDCA). The corresponding experimental results also show that the CuO and Co<SUB>3</SUB>O<SUB>4</SUB> surfaces are promising candidate catalysts.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CuO(1 1 1) and Co<SUB>3</SUB>O<SUB>4</SUB>(1 1 0) surfaces catalyze the oxidation of 5-hydroxymethylfurfural (HMF). </LI> <LI> Initial binding was formed through bridged-adsorption with O atoms in HMF. </LI> <LI> Oxidation reaction proceeds through the OH bond breaking pathway. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Schematic potential energy diagram for the formyl CH and hydroxyl OH bonds of HMF dissociation on CuO(1 1 1) and Co<SUB>3</SUB>O<SUB>4</SUB>(1 1 0) surfaces. Obviously, the hydroxyl OH bond breaking is easier than that of the formyl CH bond on the two surfaces, which indicates the first step of oxidation of HMF to FDCA should be hydroxyl OH bond breaking.</P> <P>[DISPLAY OMISSION]</P>

An Empirical Study on the Market Decision-Making ㅡ Mechanism of Chinese Peasant Consumers

Jun-yi Ren 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

Consumers from all walks of life may show different purchasing orientations. Decision-making mechanism is utilized to analyze consumption psychology of Chinese peasant consumers, their purchase behaviours and product cognition etc. This orientation has the role of psychological compulsion that consumer may not be aware when they make the purchase decision, while the role of psychological compulsion essentially dominates their decisions. To analyze psychological orientation and decision-making mechanism utilizing factor analysis, cluster analysis, and consumption psychology. Meanwhile, when income reaches some degree, income doesn’t have obvious effect on the decision-making mechanism of peasants’ purchase on durable goods, the category of consumer style depends on the “individual” factor of peasants’ consumption psychology.

Optimization of chemico-physical transformation methods for various bacterial species using diverse chemical compounds and nanomaterials

Ren, Jun,Na, Dokyun,Yoo, Seung Min Elsevier 2018 Journal of biotechnology Vol.288 No.-

<P><B>Abstract</B></P> <P>Bacterial transformation is a fundamental technology to deliver engineered plasmids into bacterial cells, which is essential in industrial protein production, chemical production, <I>etc</I>. Previously, we developed a simple chemico-physical transformation method that can be applied to various bacterial species. Here, to accelerate the advance of bacteria biotechnology we optimize our method by combinatorially evaluating chemical compounds (rubidium chloride, lithium acetate, cesium chloride, dimethyl sulfoxide, and magnesium chloride) for increasing membrane permeability and nanomaterials (sepiolite, gold(III) chloride, multiwalled carbon nanotube, and chitosan) for piercing the membranes. The best transformation efficiencies were achieved as follows; 2.84 × 10<SUP>4</SUP> CFU/μg DNA in <I>Lactococcus lactis</I> subsp. <I>lactics</I> (0.1 M CsCl and gold(III) chloride), 3.60 × 10<SUP>4</SUP> CFU/μg DNA in <I>Enterococcus faecalis</I> (1 M Li-acetate and MWCNT), 2.41 × 10<SUP>4</SUP> CFU/μg DNA in <I>Bacillus</I> sp. (0.01 M RbCl and sepiolite), 3.49 × 10<SUP>4</SUP> CFU/μg DNA (0.1 M RbCl and gold(III) chloride) in <I>Ralstonia eutropha</I> (also known as <I>Cupriavidus necator</I>) and 8.78 × 10<SUP>4</SUP> CFU/μg DNA (1 M RbCl and chitosan) in <I>Methylomonas</I> sp. DH-1. The efficiencies are up to 100-fold higher than those without optimization. Accordingly, our fast and simple chemico-physical transformation with chemical–nanomaterial optimization allows for the efficient DNA entry into various bacterial cells with high efficiency.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A chemico-physical transformation method with high efficiency was developed. </LI> <LI> RbCl, LiAc, CsCl, DMSO, and MgCl2 were used for increasing membrane permeability. </LI> <LI> Sepiolite, gold(III) chloride, MWCNT, and chitosan were used for piercing the membranes. </LI> <LI> High efficiency was achieved by optimizing chemical–nanomaterial combinations. </LI> <LI> The efficiencies are up to 100-fold higher than those without optimization. </LI> </UL> </P>

Enhancement of the solubility of recombinant proteins by fusion with a short-disordered peptide

Ren Jun,Hwang Suhee,Shen Junhao,Kim Hyeongwoo,Kim Hyunjoo,Kim Jieun,Ahn Soyoung,Kim Min-gyun,Lee Seung Ho,Na Dokyun 한국미생물학회 2022 The journal of microbiology Vol.60 No.9

In protein biotechnology, large soluble fusion partners are widely utilized for increased yield and solubility of recombinant proteins. However, the production of additional large fusion partners poses an additional burden to the host, leading to a decreased protein yield. In this study, we identified two highly disordered short peptides that were able to increase the solubility of an artificially engineered aggregationprone protein, GFP-GFIL4, from 0.6% to 61% (D3-DP00592) and 46% (D4-DP01038) selected from DisProt database. For further confirmation, the peptides were applied to two insoluble E. coli proteins (YagA and YdiU). The peptides also enhanced solubility from 52% to 90% (YagA) and from 27% to 93% (YdiU). Their ability to solubilize recombinant proteins was comparable with strong solubilizing tags, maltosebinding protein (40 kDa) and TrxA (12 kDa), but much smaller (< 7 kDa) in size. For practical application, the two peptides were fused with a restriction enzyme, I-SceI, and they increased I-SceI solubility from 24% up to 75%. The highly disordered peptides did not affect the activity of I-SceI while I-SceI fused with MBP or TrxA displayed no restriction activity. Despite the small size, the highly disordered peptides were able to solubilize recombinant proteins as efficiently as conventional fusion tags and did not interfere with the function of recombinant proteins. Consequently, the identified two highly disordered peptides would have practical utility in protein biotechnology and industry.

Evaluation of Various Escherichia coli Strains for Enhanced Lycopene Production

( Jun Ren ),( Junhao Shen ),( Thi Duc Thai ),( Min-gyun Kim ),( Seung Ho Lee ),( Wonseop Lim ),( Dokyun Na ) 한국미생물생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.7

Lycopene is a carotenoid widely used as a food and feed supplement due to its antioxidant, anti-inflammatory, and anti-cancer functions. Various metabolic engineering strategies have been implemented for high lycopene production in Escherichia coli, and for this purpose it was essential to select and develop an E. coli strain with the highest potency. In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic engineering, and further, that MG1655 is a potent host for producing lycopene and other carotenoids with the same lycopene biosynthetic pathway.