Pseudomonas putida 378이 생산하는 Lipoprotein lipase의 성질

정명주,박형숙 慶星大學校 環境問題硏究所 1993 環境硏報 Vol.3 No.1

Pseudomonas putida 378이 생산하는 LPL을 정제하여 이에 대한 몇 가지의 생물화학적 성질을 검토하여 다음과 같은 결과를 얻었다. 1. 배양액으로부터 ethanol 분획, Ammonium Sulfate 침전, DEAE-Cellulose column chromatography, CM-Sephadex column chromatography, Gel filtration를 거쳐 LPL을 정제하였다. 이때 효소는 122배 정제되었고, 회수율은 11.77%였다. 2. 정제된 효소는 SDS-PAGE에서 단일 band를 보여주어, 순수하게 정제되었음을 나타내었고, 분자량은 Gel filtration법에 의해 약 108,000으로 나타났고, SDS-PAGE에 의하여 약 107,000으로 나타났으므로, 본 효소액은 monomer로 생각된다. 3. 효소의 최적 pH는 7.0이며, 최적온도는 45℃이었으며, pH는 5.0~8.0 범위에서 안정하였고, 온도는 60℃까지 안정하였다. 4. 본 효소는 1mM Ca 에 의해 활성화 되며, 10mM Hg , Ni 에 의해 저해되었다. 5. 본 효소의 각종 지질에 대한 가수 분해율은 대조구인 olive oil에 비해 lecithin에 대해 다소 높았으며, stearic aid와 tributyrin에 대해서는 매우 낮았다. The purification and properties of the lipoprotein lipase from Pseudomonas putida 378 were investigated. It was purified by ethanol, ammonium sulfate fractionation and chromatographies on DEAE- Cellulose, CM-Sephadex and gel filtration of Sephadex G-100. The enzyme was purified about 122 fold with a yield of 11.77%. The purified enzyme showed a single protein band on SDS-PAGE. Its molecular weight was estimated to be about 108,000 and 107,000 by gel filtration and SDS-PAGE, respectively. Lipoprotein lipase had an optimum pH of 7.0, and an optimal temperature of 45℃, and the stable range of pH was 5.0 to 8.0. The enzyme was thermostable at below 60℃. The enzyme was strongly inhibited by Hg (10mM), Ni (10mM) and showed the most activity ohn Ca (1mM). This lipoprotein lipase was capable of hydrolyzing a variety of oils, showing were active on lecithin than on olive oil.

P3-F계 세제의 살균력 측정

정명주,손태문,박형숙 慶星大學校 1998 論文集 Vol.19 No.2

The microbial activity of P3-luboklar FC,FC-A,FC-A(XT)DX and DXB was compared by DGHM Germ Carrier Test method. The following results were obtained. 1. FC,KB and KC has shown to be effective against all strains within 10 minutes. 2. FC-A was effective against E. coli, immediately, and kill could be achieved against Pseudomonas aeruginosa and Staphylococcus aureus within 4 minutes and 16 minutes, respectively. 3. DXB was effective against all strains within 10 minutes with the exeption of B. subtilis. 4. FC-A(XT) was the most effective against B. subtilis.

고호염성 원시세균 Haloarcula sp. EH-1에 의한Poly-3-hydroxyalkanoate 생산을 위한 배양 조건

정명주,박형숙 慶星大學校 1998 論文集 Vol.19 No.1

Effect culture conditions on Poly-β-Hydroxyalkanoates(PHA) production in Haloarcular sp. EH-1 were investigated. The optimal temperature and pH for PHA production in Haloacular sp. EH-1 were 38℃ and 7.0, respectively. The optimal medium compositions for cell growth comprised glucose 1%, NH₄CI 0.2%, NaCI 25%, KH₂PO₄ 0.08%, MgCI2 0.5%, MgSO₄ 2.0%, CaCl₂ 0.3%, KCl 0.5%, NaHCO₃ 0.02%, NaBr 0.05%, and FeCl₃ 0.0002%. The optimal conditions for PHA production are identical those for cell growth.

철도교통소음의 수평·수직적 전달 및 감쇠 특성

최형일,정원삼,김난희,강영주,위환 조선대학교 환경공해연구소 2006 環境公害硏究 Vol.19 No.1

This study has been conducted to achieve the following objectives: First, in order to understand the horizontal propagation and attenuation characteristics for the railroad traffic noise, we selected actively developed areas within 100 meters away from the road to measure the level of traffic noise near a road. To measure the noise near a railroad, we selected areas within 100 meters away from the railroad and then selected Saemaul-ho and Mugoongwha-ho as the subjects for our experiment, In this way, we analyzed the horizontal propagation and attenuation characteristics for the traffic noise occurring in diversified areas. Second, in order to understand the vertical propagation and attenuation characteristics for the railroad traffic noise, we measured and analyzed the distributional characteristics of vertical sound pressure levels on each floor of multi-storied apartment buildings according to changes of traffic load and types, and the existence or nonexistence of soundproof walls. For the case of the railroad traffic noise, we also selected Samaul-ho and Mugoongwha-ho as the subjects for our experiment, and we measured and analyzed the different noise levels on each floor of multi-storied apartment buildings from the soundproof wall. The results of Horizontal propagation and attenuation characteristics for the railroad traffic noise are as follows: In cases of the flat land, cutting land, and bridge area, as distance increases, the sound pressure level steadily decreases. The cutting land has an especially high distance decrease compared to the other areas, and the sound pressure level for the bridge area is higher than that of the flat land with a measurement of 9.7~17.5㏈(A). Vertical propagation and attenuation characteristics for the railroad traffic noise are as follows: The amount of sound pressure level decrease is 13.9~15.4㏈(A) for Samaul-ho and 10.9~11.5㏈(A) for Mugoongwha-ho when measuring the vertical sound pressure levels at heights lower than 4.5m, which indicates a fairly large decrease. At 6m, the amount of decrease is 7.3㏈(A) for Samaul-ho and 5.0㏈(A) for Mugoongwha-ho, which indicates a small decrease.

축압축을 받는 CFRP 적층부재의 에너지흡수특성과 파괴모드에 관한 연구

김정호,정회범,전형주 한국산업안전학회 2002 한국안전학회지 Vol.17 No.3

The object of this paper is to investigate collapse characteristics of CF/Epoxy(Carbon Fiber/Epoxy resin)composite tubes on the change of interlaminar number and fiber orientation angle of outer and to evaluate reappearance of collapse characteristics on the change of tension strength of fibers under static and impact axial compression loads. When a CF/Epoxy composite tube is crushed, static/impact energy is consumed by friction between the loading plate and the splayed fronds of the tube, by fracture of the fibers, matrix and their interface. In general, CF/Epoxy tube with 6 interlaminar number(C-type) absorbed more energy than other tubes(A, B, D-types). The maximum collapse load seemed to increase as the interlaminar number of such tubes increases. The collapse mode depended upon orientation angle of outer of CF/Epoxy tubes and loading status(static/impact). Typical collapse modes of CF/Epoxy tubes are wedge collapse mode, splaying collapse mode and fragmentation collapse mode. The wedge collapse mode was shown in case of CF/Epoxy tubes with 0˚ orientation angle of outer under static and impact loadings. The splaying collapse mode was shown in only case of CF/Epoxy tubes with 90˚ orientation angle of outer under static loadings, however in impact tests those were collapsed in fragmentation mode. So that, CF/Epoxy tube with 6 interlaminar number and 90˚ outer orientation angle presented to the optimal collapse characteristics.

Cadmium이 넙치(Paralichthys olivaceus)의 면역 반응에 미치는 영향

변주영,유민호,전려진,이형호,정현도 한국어병학회 2001 한국어병학회지 Vol.14 No.2

어류의 면역반응에 대한 cadmium (Cd)의 영향을 분석하기 위하여 넙치 (Paralichthys olivaceus)를 각기 다른 방향으로 Cd에 노출시킨 후 특이적 면역반응의 변화와 Edrwrdsiella tarda KFE (E. tarda KFE)의 인위감염에 대한 저항성을 분석하였다. E. tarda KFE의 formalin killed cell (FKC)로 면역시키기 2주전부터 계속하여 실험 기간동안 침지법으로 Cd(20ppb)에 노출된 시험구는 노출되지 않은 시험구와 양성 대조구보다 혈청 내 특이 항체가가 빠르게 최고치에 이르렀으나, 감소속도는 노출시키지 않은 양성 대조구에 비하여 빠른 것으로 나타났다. 이러한 경향은 splenocytes를 ELISPOT-assay (enzyme-linked immunospot assay)를 이용하여 특이 항체 생성 세포 (specific antibody secreting cell, SASC) 수를 분석해 보았을 때에도 동일하게 나타났다. 그러나 면역 전 2주 동안만 Cd에 노출시킨 시험구에서는 혈청내 항체생성 결과와는 달리 증가된 SASC의 수를 보여 주었다. 그리고 면역 2주 전부터 실험 전기간동안 계속해서 Cd에 노출시킨 넙치를 대상으로 하여 E. tarda FKC 생균으로 인위 감염시켰을 때 100% 폐사율을 보여 주었다. 이것은 Cd에 지속적으로 노출된 어류에서의 방어 체계는 면역반응뿐만 아니라 독성효과도 함께 고려되어야 하는 복합적인 것임을 확인 할 수 있었다. Olive flounder, Paralichthys olivaceus known as an one of the major aquacultured species in Korea were exposed to cadmium(Cd) with different protocols and analyzed the effects of exposure on the immune response. Antibody levels in sera of the group exposed to Cd(20ppb) by immersion method from 2 weeks before immuniztion with formalinised Edwardsiella tarda(E. tarda) KFE entigen to the end of experiment reached to peak level faster than that of the non-exposed group. After this peaking time the levels decreased much at a faster rate compared to the non-exposed group. This tendency was also appeared in the numbers of specific antibody secreting cells(SASC) analyzed with the enzyme-linked immunospot (ELISPOT)-assay technique in the splenocytes of the experimental groups exposed to Cd with different ways. Interestingly, the group exposed to Cd for 2 weeks before immunization also showed increased numbers of SASC unlikely the antibody production and suggested a more critical influence of cadmium exposure in early stage of immune reaction. Artificial infection with live E. tarda KFE induces 100% mortality in the flounder exposed to cadmium throughout the experimental period from two weeks before the immunization. It may imply that some other factors related to specific immunity are involving in the defence system of flounder exposed to Cd. Taked together. Cd exposure may induce temporaily stimulatory or indhibitory effects on the immune reaction, but suppress the physiological systems for the resistant against the infective agents with other toxic effects.

천일염으로부터 고호염균의 및 동정

빅형숙,정명주 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

시판 천일염으로부터 고호염성균을 분리하여 동정하였다. 분리균주는 그람음성, 통성혐기성으로 운동성을 나타내었다. 배양적 특성은 콜로니에 형태가 둥글고 볼록하였고, 지름은 약 2mm 정도이며, red-orange색을 나타내었다. Mg^2+ ion의 농도에 따라서 간상 또는 구형으로 다형태성을 나타내었고, 생육온도 범위는 35~45℃, 생육 pH 범위는 7.0~9.0이었다. 생육은 40℃에서 NaCl의 농도가 4.3~5.0 M에서 최대를 나타내었다. 생리적 특성으로 catalase, oxidase 양성이며, 전분을 분해하였고, bacitracin 감수성을 나타내었다. 다양한 당이용성를 나타내며, DNA의 G+C 함량은 62.7 mol%이었다. 이상의 결과로부터 분리균주는 Haloarcula 속으로 동정되어 Haloarcula sp. EH-1으로 명명하였다. An Extremely halophilic bacterium was isolated from solar salts. The isolated strain was Gram-negative, facultatively anaerobic, and motile bacterium. The colony was circular, smooth, and red-orange color. The strain showed pleomorphism depending on magnesium ion concentrations. The range of temperature and pH for growth of the isolate were 35~45℃ and 7.0~9.0. Nacl concentration for growth of it was 4.3~5.0 M. The isolate was catalase and oxidase positive, and sensitive to bacitracin. It showed starch hydrolyzing and acid forming characteristics. DNA G+C content was 62.7 mol%. The morphological, physiological and biochemical characteristics of the isolate resembled those of the Haloarcula vallisumortis, therefore it was identified as Haloarcula sp. EH-1.

급성 척수허혈 및 재관류손상에 대한 Na^+/H^+ 교환억제제(DMA)의 보호효과 : 가토 척수의 허혈 및 재관류손상 모형에 의한 실험적 연구 An Experimental Study in Spinal Cord Ischemia and Reperfusion Injury Model

고정관,박형주,이길노 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Spinal cord injury after operations on the descending thoracic and thoracoabdominal aorta remains a persistant clinical problem. Previous attemps to decrease the risk of this devastating complication by lowering the rate of metabloism of the spinal cord have met with varying success. We hypothesized that the tolerance of the spinal cord to an ischemic insult could be improved by means of Na^+/H^+ exchange inhibitor(DMA). DMA prevent intracellular Na^+ influx and maintain intracellular acid pH during ischemia by blocking the Na^+/H^+ exchange system. Additionally, subsequent Ca^++ influx during reperfusion is prevented by blocking the Na^+/Ca^++ exchange as well. It is well established that not only the intracellular acidosis during ischemia prolongs cellular survival but almost all of the reperfusion injury is associated with intracellular Ca^++ overload. Twenty four New zealnad white rabbits underwent 30 minutes of isolated infrarenal aortic occlusion after heparin anticoagulation. Clamps were placed both below the left renal vein and above the aortic bifurcation. In 12 rabbit(Amiloride Group), a bolus of amiloride(3㎎/㎏) intravenous injection and a bolus of diluted amiloride(100uM) was infused the isolated aortic segment immediately after crossclamping and immediately before release of clamping. In another control group(12 rabbits), the aortic segment was flushed with normothermic saline in a fashion identical to that of the study group. The aortic clamps were removed after 30 minutes, the abdomen was closed, and the animals were allowed to recover from anesthesia. Spinal cord function was assessed 72 hours after operation by the Talov's scale. All animals were put to death at 72 hours after operation and spinal cords were harvested for MDA and histologic analysis. The spinal cord function of all Amiloride group animals were fully intact with Talov's scores of 3-5; control group animals were all paraplegic with Talov's score of 0-2(p<0.001, student t-test). MDA level in Amiloride group was 0.046±0.06 and control group was 0.065±0.01(p<0.0001, student t-test). Histologic examination of spinal cords from Amiloride gropup rabbits revealed a little evidence of cord injury, whereas spinal cords from control group had evidence of extensive cord injuty with central gray necrosis, axonal swelling, dissolution of Nissl substance, and astrocyte and macrophage infiltration. Systemic and regional infusion of the crossclamped infrarenal rabbit aorta with Na^+/H^+ exchange inhibitor(DMA) nearly complete prevented paraplegia in our model despite a 30-minute ischemic insult.

마그네슘, 칼륨 및 기타의 금속이온이 Haloarcula sp. EH-1의 생육과 형태에 미치는 영향

박형숙,정명주 경성대학교 1997 論文集 Vol.18 No.1

Effect of magnesium, potassium, and some metal ions on the growth and morphology of Haloarcula sp. EH-1 were investigated. Maximum growth of the Haloarcula sp. EH-1 occured in the presence of 0.1 to 0.5 M magnesium ion and the production of carotenoid was directly proportional to the growth of this organism. Above 0.08 Mg?? the majority of cells were rod forms but below 0.03 Mg?? it became sphere forms. The growth of Haloarcula sp. EH-1 was stimulated by 10 ppm iron, 70 ppm calcium, and 40 ppm cupper ions, respectively.

백서에서 Depulpin®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However, the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it may also alter the pulp tissue rendering it immumologically active, and have carcinogenic potential. Recently Depulpin®(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier. But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine. Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue, in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present. it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin® group The area of necrotic tissue which had no cells and fibers , was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nucleli. A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resorption and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall. was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.