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Ju-Hua Wang,Xiu-Heng Xue,Jie Zhou,Cai-Yun Fan,Qian-Qian Xie,Pan Wang 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.3
Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of Ca<SUP>2+</SUP>, Mg<SUP>2+</SUP>, K<SUP>+</SUP>, and HCO₃<SUP>-</SUP> in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.
H∞ Switching Filter Design for LPV Systems in Finite Frequency Domain
Heng Wang,He-Hua Ju,Yu-Long Wang 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.3
This article studies the problem of finite frequency H∞ filter design for switching linear parameter varying (LPV) systems with disturbance frequency affected by system parameters. A set of switching filters are designed, each suitable for a specific parameter subregion and a specific finite frequency performance index. A hysteresis switching logic is adopted, and the design problem is finally formulated into linear matrix inequality (LMI) which can be solved efficiently with LMI Control Tool-box. A numerical example is given to illustrate the effectiveness of the proposed method.
Seung-Ju Hwang,Chang-Gue Son,Jing-Hua Wang 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The global prevalence of nonalcoholic fatty liver disease (NAFLD) is estimated to be 25% and has continued to increase; however, no drugs have been approved for NAFLD treatments. The root of CA (blind) has been frequently used in oriental medicine as an antifebrile, diuretic, antidote and for curing rheumatologic and scrofula in eastern Asia. The intake of high-fat and fructose diet (HFHFD) for 10-weeks remarkedly increased body, liver and fat weight, and serum aspartate transaminase (AST), alanine transaminase (ALT), triglyceride (TG), and hepatic TG levels. Yet, Extracts of CA (CAE) relieved the alteration of almost parameters compared with the HFHFD group. Furthermore, CAE attenuated the expression of lipid metabolism-related protein, and inflammation-related parameters compared with HFHFD. Taken together, these results show that CAE might be a potential therapeutic agent for regulating a wide spectrum of NAFLDs from steatosis to hepatitis via actions on lipid metabolism-related proteins.
Jing-Hua Wang,Seung-Ju Hwang,Chang-Gue Son 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
As a medicinal herb, CA contains lots of active components and possesses various pharmacological activities. Recently l CA has been frequently applied in cosmetics for skin whitening. However, there are still many issues to be figured out, like consistent effects and unknown mechanisms. This study aimed to demonstrate the anti-melanogenesis activity of hexane fraction of CA (HCA) on UVB-induced skin hyperpigmentation. C57BL/6j mice were irradiated by UVB (100 mJ/cm², 5 times/week) for 4 weeks. HCA was applied to the left ear after irradiation for 8 weeks, and the right ear was used as an inter control. According to the gray value and Mexameter melanin index, HCA reduced melanin in ear skin compared to all controls. HCA notably declined melanin generation in αMSH-triggered B16/F10 melanocyte in a concentration-dependent manner. HCA treatment also markedly diminished the tyrosinase activity, gene expression, and protein level in B16/F10. Cellular cAMP and MITF were noticeably down-regulated by HCA. Consequently, we suggested that CA exerts an effect of anti-melanogenesis via reduction of tyrosinase that might be mainly mediated by regulating cAMP cascade and MITF pathway.
You-Hua Cai,Ze-Xin Liang,Shuang Li,Ming-Jun Zhu,Zhenqiang Wu,Shang-Tian Yang,Ju-Fang Wang 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6
There is an increasing worldwide interest in bioethanol production from agricultural, industrial, and urban residues for both ecological and economic reasons. The acid hydrolysis of cassava pulp to reducing sugars and their fermentation to ethanol were evaluated in a fibrousbed bioreactor with immobilized Δldh, a genetically engineered Thermoanaerobacterium aotearoense. A maximum yield of total reducing sugars of 53.5% was obtained after 8 h of hydrolysis at 85oC in 0.4 mol/L hydrochloric acid with a solid-to-liquid ratio of 1:20, which was optimized by using an orthogonal design based on preliminary experiments. In the FBB, the fed-batch fermentation, using glucose as the sole carbon source, gave a maximum ethanol production of 38.3 g/L with a yield of 0.364 g/g in 100 h; whereas the fed-batch fermentation, using xylose as the sole carbon source, gave 34.1 g/L ethanol with a yield of 0.342 g/g in 135 h. When cassava pulp hydrolysate was used as a carbon source, 39.1 g/L ethanol with a yield of 0.123 g/g cassava pulp in185 h was observed, using the fed-batch fermentation model. In addition, for repeated batch fermentation of cassava pulp hydrolysate carried out in the fibrous-bed bioreactor, long-term operation with high ethanol yield and volumetric productivity were achieved. The above results show that the acid hydrolysate of cassava pulp can be used for ethanol production in a fibrous-bed bioreactor, although some inhibition phenomena were observed during the process of fermentation.
Kuo-Feng Hua,A-Ching Chao,Ting-Yu Lin,Wan-Tze Chen,Yu-Chieh Lee,Wan-Han Hsu,Sheau-Long Lee,Hsin-Min Wang,Ding-I. Yang,Tz-Chuen Ju 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.4
Background: Huntington's disease (HD) is a neurodegenerative disorder caused by the expansion oftrinucleotide CAG repeat in the Huntingtin (Htt) gene. The major pathogenic pathways underlying HDinvolve the impairment of cellular energy homeostasis and DNA damage in the brain. The protein kinaseataxia-telangiectasia mutated (ATM) is an important regulator of the DNA damage response. ATM isinvolved in the phosphorylation of AMP-activated protein kinase (AMPK), suggesting that AMPK plays acritical role in response to DNA damage. Herein, we demonstrated that expression of polyQ-expandedmutant Htt (mHtt) enhanced the phosphorylation of ATM. Ginsenoside is the main and most effectivecomponent of Panax ginseng. However, the protective effect of a ginsenoside (compound K, CK) in HDremains unclear and warrants further investigation. Methods: This study used the R6/2 transgenic mouse model of HD and performed behavioral tests,survival rate, histological analyses, and immunoblot assays. Results: The systematic administration of CK into R6/2 mice suppressed the activation of ATM/AMPK andreduced neuronal toxicity and mHTT aggregation. Most importantly, CK increased neuronal density andlifespan and improved motor dysfunction in R6/2 mice. Conversely, CK enhanced the expression of Bcl2protected striatal cells from the toxicity induced by the overactivation of mHtt and AMPK. Conclusions: Thus, the oral administration of CK reduced the disease progression and markedlyenhanced lifespan in the transgenic mouse model (R6/2) of HD.
Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.
Xu Zhang,Qing-Hua Deng,Jian-Hua Deng,Sheng-Ju Wang,Qiu Chen 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.2
Ulcerative colitis (UC) is associated with intestinal immune imbalance and inflammatory response. Because dehydrolovastatin (DLVT), a derivative of lovastatin, has been recently shown to inhibit inflammation and relieve immune arthritis induced by chemical stimuli, we studied its effect and possible mechanism on UC induced by dextran sulfate sodium. The BALB/c mice were classified into six groups: normal control group, model group, DLVT high dose group, DLVT low dose group, salazosulfapyridine (SASP) group and lovastatin (LVT) group. The disease activity indices of UC and pathological changes were investigated. The myeloperoxidase (MPO) activity in colon tissue and inflammatory factors such as IL-6, IL-10, IL-17, and TNF- in the serum were analyzed by ELISA, while the expression of NF-B p65 protein in colon tissue was detected by immunohistochemistry and western blot. DLVT relieved the disease activity indices and pathological damage of the UC mice. Furthermore, DLVT significantly decreased MPO activity and improved the imbalance of inflammatory cytokines through inhibiting the expression of NF-B p65. Meanwhile, the positive drug of SASP has a similar effect to DLVT, but the effect of DLVT in both decreasing IL-17, TNF-, and increasing IL-10 was significantly stronger than that of SASP. These results suggest that DLVT may ameliorates the symptoms of UC.