흰쥐 시상에서 Epidermal growth factor receptor면역반응 신경세포의 생후 발달에 관한 연구

박영란,정윤영,김종중,문정석,오재욱,정영욱,김주수 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Background and Objectives : Epidermal growth factor receptor (EGFR), a 170-kDa transmembrane glycoprotein, appears to mediate epidermal growth factor (EGF) activity. Transforming growth factor-α and EGF produce their biological effects in numerous systems by stimulating the EGFR In this study, we examine the postnatal development of EGFR immunoreactivity in the different regions of the thalamus of the rat Materials and Methods : The present study is based on 28 postnatal cases of rat thalamus ranging from the day of birth, postnatal day 0 (P0) to 30 days (P3, P5, P10, P15, P20, P30), and these cases were compared with adult rat thalamus. Cryostat sections were processed free-floating with monoclonal antibody by immunohistochemistry Results : EGFR immunoreactivity in the thalamus of the rat showed very different patterns according to postnatal ages and thalamic areas. EGFR-immunoreactive cells appeared in the first two postnatal weeks, except the ventral posterior thalamic nuclei. In the early postnatal days, EGFR-immunoreactive cells appeared thalamic midline structures, increased progressively in the first two postnatal weeks, and followed mediolateral gradient. The mature patterns of EGFR-immunoreactive cells were achieved at P20 Conclusion : These data indicate that the maturation of EGFR-immunoreactive cells requires a relatively prolonged period of time to achieve an adult configuration. Many growth factors probably play protective or neurotrophic roles at EGFR-immunoreactive neurons of thalamus both young and adult rats In addition to difference in time of appearance in thalamic nuclei and developing pattern with mediolateral gradient suggest that EGFR-immunoreactivities are correlated with the appearance of the related functional.

국가디지탈도서관 구축계획에 관한 연구

한상완,김성혁,문성빈,이란주 한국문헌정보학회 1996 한국문헌정보학회지 Vol.30 No.1

21세기 국가 정보력 제고를 위해서 세계 선진국가들은 국가디지털도서관 구축을 위한 전략과 막대한 예산을 투입하고 있다. 우리나라도 국가디지털도서관을 시급히 구축하여 다양한 멀티미디어 정보를 국민에게 제공할 수 있어야만 국가발전경쟁에서 선진국과 어깨를 나란히 할 수 있다. 따라서 이 연구에서는 우리나라의 국가디지털도서관 구축 방안을 마련하기 위해 우선 선진 외국의 디지털도서관의 구축사례를 연구한 다음 이를 바탕으로 우리나라 국가디지털도서관의 건축, 시설, 장비, 조직과 인력, 각종 사업과 기능 등을 소요예산과 함께 가능한한 구체적으로 연구 제시하였다 Many of the development countries in the world have been investing a lot of money and developing plans for a national digital library in order to secure the information power in the 21st century. In order for our country to compete with others, it is also urgent to build a National Digital Library in Korea, which can provide a variety of multimedia information for the people. In this study, based on the investigation of the several cases of the national digital libraries in the developed countries, we suggest a detailed plan for the National Digital Library which includes building, facilities, equipments, organization, staff, events, functions, and expected budget

모체 Thyroxine 투여가 태아알코올효과를 가진 흰쥐 대뇌겉질 및 해마에서 NPY함유 신경세포의 생후 발달에 미치는 영향

김복,박상기,박영란,김종중,문정석,김주수,문영민,현영식,천관영,정윤영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives: Maternal alcohol abuse is considered to be one of the most prominent cause of neurobiological malformations in the postnatal and adult life of the offspring. In this study, we investigated the effects of maternal alcohol drinking on the postnatal development of NPY-containing neuron, and, the influence of thyroxine treatment on the cerebral cortex and hippocampus of pups of alcohol abused mother. Materials and Method: Time-pregnant rats were divided into three groups. Alcohol-fed group A received 35 calories of liquid alcohol diet daily from gestation day 6; control pair-fed group B was fed a liquid diet in dextrin replaced alcohol isocalorically: alcohol + T4 group C received 35 calories liquid alcohol diet and exogenous thyroxine subcutaneously. Results: Group C showed prominent NPY immunoreactivity in the cerebral cortex compared to group A and B at P7. In group C, NPY-containing neurons were widely distributed in the all layers of cerebral cortex after P14. Besides, numerical decrease of NPY-containing neuron as age increases was not found in group C. However, the decrease of NPY-containing neuron was clearly observed in group A compared to group C after P14. In hippocampus, group Band C were appeared similar patterns after P7. Additionally, in group C, NPY immunoreactivity was prominently appeared in CA2 and CA3 at P14 as compared to group B. Conclusions: The present results showed the increase of intensity and number of NPY-containing neurons in cerebral cortex and hippocampus of pups of exogenous T₄ supplemented alcohol-exposed dams as compared to control pair-fed and alcohol-exposed pups at P7. It presumably suggest that NPY-containing neurons might be regulated by the early postnatal growth stimulatory effect of the exogenously supplemented T₄. Therefore, the increase of NPY synthesis caused by maternal administration of exogenous thyroxine may ameliorate fetal alcohol effect, one of the ill effects as a result of the dysthyroid state following maternal alcohol abuse.

Fish Myogenic Regulatory Protein LUC7L: Characterization and Expression Analysis in Korean Rose Bitterling (Rhodeus uyekii)

Kim, Ju Lan,Kong, Hee Jeong,Kim, Hyung Soo,Kim, Woo-Jin,Kim, Dong-Gyun,Nam, Bo-Hye,Kim, Young-Ok,An, Cheul Min The Korean Society of Developmental Biology 2014 발생과 생식 Vol.18 No.4

Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designated RuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-rich region at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other species including human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression of RuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA was detected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Further investigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.

Genomic cloning and characterization of the Korean rose bitterling Rhodeus uyekii beta-actin gene

Ju Lan Kim,Eun-Ha Shin,Woo-Jin Kim,Hyung Soo Kim,Bong-Seok Kim,Cheul Min An,Hee Jeong Kong 한국수산과학회 양식분과 2014 한국수산과학회 양식분과 학술대회 Vol.2014 No.7

Fish Myogenic Regulatory Protein LUC7L: Characterization and Expression Analysis in Korean Rose Bitterling (Rhodeus uyekii)

Ju Lan Kim,Hee Jeong Kong,Hyung Soo Kim,Woo-Jin Kim,Dong-Gyun Kim,Bo-Hye Nam,Young-Ok Kim,Cheul Min An 한국발생생물학회 2014 발생과 생식 Vol.18 No.4

Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designated RuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-rich region at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other species including human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression of RuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA was detected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Further investigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.

A simple method to evaluate body condition score to maintain the optimal body weight in dogs

( Ju Lan Chun ),( Han Tae Bang ),( Sang Yun Ji ),( Jin Young Jeong ),( Minji Kim ),( Byeonghyeon Kim ),( Sung Dae Lee ),( Yoo Kyung Lee ),( Kondreddy Eswar Reddy ),( Ki Hyun Kim ) 한국축산학회 2019 한국축산학회지 Vol.61 No.6

Overweight and obesity induce serious health problems that exert negative effects on dog’s welfare. Body condition score (BCS) is a common method to evaluate the body fat mass in animals. By palpating and observing fats under the skin it is possible to predict animal’s body fat accumulation condition. BCS is also a useful tool to estimate body fat composition in dogs. However, BCS can be subjective when it was performed by non-professionals like pet’s owners. To develop a method to avoid the misevaluation of BCS twenty-four Beagles were enrolled and performed BCS evaluation. In addition, the length of chest and abdominal girths were measured. In correlation analysis, the sizes of chest and abdominal girth were significantly correlated with BCS. Especially, the difference and ratio of the chest and abdominal length were highly correlated with the BCS. With that, we suggested that this simple measurement of chest and abdominal girths by a measuring tape would be an effective method to estimate BCS scores in dogs that helps non-professionals to manage their own dog’s nutritional condition by monitoring body fat accumulation condition.

Comparison of sample preparation procedures of inductively coupled plasma to measure elements in dog’s hair

( Ju Lan Chun ),( Han Tae Bang ),( Sang Yun Ji ),( Jin Young Jeong ),( Minji Kim ),( Byeonghyeon Kim ),( Sung Dae Lee ),( Yoo Kyung Lee ),( Kondreddy Eswar Reddy ),( Ki Hyun Kim ) 한국축산학회 2020 한국축산학회지 Vol.62 No.1

The pre-treatment condition affects on the element analysis of inductively coupled plasma (ICP). In this study the pre-treatment condition of ICP has been studied to quantify elements in dog’s hair. The hair samples were collected from twelve female Beagles by clipping them into 1 or 2 cm at the back neck. The qualitative and quantitative analysis of elements in hairs were performed by using ICP. By ICP nine elements were qualitatively detected and quantitatively analyzed (Ca, Cu, Fe, K, Mg, Na, P, Se, Zn). The measured amounts of elements were compared between 3 step and 2 step procedures which were with and without the acetone based washing step. The quantitative analysis showed that the concentrations of K, Na, P, and Se were significantly decreased in hair samples with acetone-based washing (p < 0.005 or 0.001) unlike those without the acetone-based washing. It implied that some minerals are lost by the acetone based washing during the sample preparation step. Therefore, the acetone based washing process is not suitable for quantifying elements in dog’s hair. In addition, the results of qualitative and quantitative analysis were compared. Although there was a difference in absolute values of elemental contents in hair, the results of qualitative and quantitative analysis were significantly correlated each other. This finding suggested that the results of qualitative analysis can be used to monitor elemental contents in dog’s hair.

Follistatins have potential functional role in Porcine Embryogenesis

Kim, Dong-Hee,Chun, Ju Lan,Lee, Ji Hye,Kim, Keun Jung,Kim, Eun Young,Lee, Bo Myeong,Zhuang, Lili,Kim, Min Kyu Institute of Agricultural Science 2016 Korean Journal of Agricultural Science Vol.41 No.2

In animal reproduction, the quality of oocytes and embryos has been evaluated by the expression of specific molecules. Follistatin (FST), which was isolated from follicular fluid, binds and bio-neutralizes the TGF-${\beta}$ superfamily members. Previous studies using the bovine model showed FST could be an important molecular determinant of embryo developmental competence. However, the effect of FST treatment on porcine embryo developmental competence has not been established. In this study, the effect of exogenous FST on porcine embryo developmental competence was investigated during in vitro culture. FST (10 ng/ml) treatment induced a significant decrease in the rate of cell arrest at the 4-cell stage. The expression levels of DNA-methyltransferase 1 (DNMT1), histone deacetylase 1 (HDAC1), and histone deacetylase 2 (HDAC2) were decreased in 4-cell stage embryos. FST treatment also resulted in significant improvements in developmental competence of embryos in terms of blastocyst formation rate and OCT-4 mRNA levels, the latter being related to pluripotency. In conclusion, during in vitro culture, FST treatment significantly ameliorated 4-cell block during embryonic development and improved embryo developmental competence. Therefore, FST treatment may potentially have a functional role in porcine embryogenesis that is broadly applicable to enhance in vitro embryo development.

Difference of gut microbiota composition based on the body condition scores in dogs

( Ju Lan Chun ),( Sang Yun Ji ),( Sung Dae Lee ),( Yoo Kyung Lee ),( Byeonghyeon Kim ),( Ki Hyun Kim ) 한국축산학회(구 한국동물자원과학회) 2020 한국축산학회지 Vol.62 No.2

Microorganism residing in the gut has been known to have important roles in the animal body. Microbes and host microenvironment are highly related with host’s health including energy metabolism and immune system. Moreover, it reported that gut microbiome is correlated with diseases like obesity in human and dogs. There have been many studies to identify and characterize microbes and their genes in human body. However, there was little information of microbiome in companion animals. Here, we investigated microbiota communities in feaces from twenty - four Beagles (aged 2 years old) and analyzed the taxonomy profile using metagenomics to study the difference among gut microbiome based on body condition score (BCS). gDNA was isolated from feaces, sequenced and clustered. Taxonomy profiling was performed based on the NCBI database. BCS was evaluated once a week according to the description provided by World Small Animal Veterinary Association. Firmicutes phylum was the most abundant followed by Bacteroidetes, Fusobacteria, Proteobacteria and Actinobacteria. That main microbiota in gut were differently distributed based on the BCS. Fusobacteria has been known to be associated with colon cancer in human. Interestingly, Fusobacteria was in the third level from the top in healthy dog’s gut microbiome. In addition, Fusobacteria was especially higher in overweight dogs which had 6 scales of BCS. Species Fusobacterium perfoetens was also more abundant when dogs were in BCS 6. It implied that F. perfoetens would be positively related with overweight in dogs. These finding would contribute to further studies of gut microbiome and their functions to improve dog’s diets and health condition.