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A Genetically Encoded Biosensor for the Detection of Levulinic Acid
Kim Tae Hyun,Woo Seung-Gyun,Kim Seong Keun,Yoo Byeong Hyeon,Shin Jonghyeok,Rha Eugene,Kim Soo Jung,Kwon Kil Koang,Lee Hyewon,Kim Haseong,Kim Hee-Taek,Sung Bong-Hyun,Lee Seung-Goo,Lee Dae-Hee 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.4
Levulinic acid (LA) is a valuable chemical used in fuel additives, fragrances, and polymers. In this study, we proposed possible biosynthetic pathways for LA production from lignin and poly(ethylene terephthalate). We also created a genetically encoded biosensor responsive to LA, which can be used for screening and evolving the LA biosynthesis pathway genes, by employing an LvaR transcriptional regulator of Pseudomonas putida KT2440 to express a fluorescent reporter gene. The LvaR regulator senses LA as a cognate ligand. The LA biosensor was first examined in an Escherichia coli strain and was found to be non-functional. When the host of the LA biosensor was switched from E. coli to P. putida KT2440, the LA biosensor showed a linear correlation between fluorescence intensity and LA concentration in the range of 0.156–10 mM LA. In addition, we determined that 0.156 mM LA was the limit of LA detection in P. putida KT2440 harboring an LA-responsive biosensor. The maximal fluorescence increase was 12.3-fold in the presence of 10 mM LA compared to that in the absence of LA. The individual cell responses to LA concentrations reflected the population-averaged responses, which enabled high-throughput screening of enzymes and metabolic pathways involved in LA biosynthesis and sustainable production of LA in engineered microbes.
Development of a novel high throughput selection method for proteins with improved solubility
Chakhee KIM,Jonghyeok SHIN,Myungseo PARK,Yunjeong PARK,Hooyeon KIM,Hye Rin KIM,Joon-Bum PARK,Byoungjae KONG,Young-Hun JUNG,Choongjin BAN,Seokoh MOON,Yuna KIM,Seok-Hyeon YU,Sora CHO,Won-Ki MIN,Dae-Hyuk 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.4
Zorro의 연관키 차분특성을 이용한 키 복구 공격 및 PGV-Zorro의 충돌쌍 공격
김기윤(Giyoon Kim),박은후(Eunhu Park),이종혁(Jonghyeok Lee),장성우(Sungwoo Jang),김지훈(Jihun Kim),김한기(Hangi Kim),김종성(Jongsung Kim) 한국정보보호학회 2018 정보보호학회논문지 Vol.28 No.5
블록암호 Zorro는 AES와 비슷한 연산과정을 거치나 S-box 개수를 줄여 부채널 공격에 대비한 마스킹을 구현비용을 줄일 수 있게 설계되었다. 하지만 마스터키를 라운드키로 그대로 사용하기 때문에 연관키 차분공격 관점에서 취약하다. 본 논문에서는 Zorro의 연관키 차분특성을 이용해 키 복구 공격을 보인다. 또한 안전성이 증명된 12가지의 PGV 모델을 Zorro를 기반으로 할 때 블록암호의 연관키 차분 특성이 해시함수 충돌쌍 공격에 어떻게 활용될 수 있는지 설명하고 실제 충돌쌍을 제시한다. The block cipher Zorro is designed to reduce the implementation cost for side-channel countermeasure. It has a structure similar to AES, but the number of S-Boxes used is small. However, since the master key is used as the round key, it can be vulnerable to related key attacks. In this paper, we show key recovery attacks on Zorro using related-key differential characteristics. In addition, the related key differential characteristics are fatal when Zorro is used as the base block cipher of the hash function. In this paper, we describe how these characteristics can be linked to collision attacks in the PGV models.