銅鑛山廢水로부터 分離한 구리 耐性菌(P.stutzeri)의 菌體內 구리 蓄積 特性

許鍾秀,曺周植,韓文圭,李洪宰,林永成 경상대학교 환경보전연구소 1994 環境保全硏究所報 Vol.2 No.1

Of the copper-tolerant 215 bacterial strains isolated from polluted river water, wastewater and activated sludges of wastewater treatment plants, or wastewater and soils of the copper mine aera in Gyeongnam province a strain, which showed considerably higher growth rate in the agar plate containing 2000ppm of copper than any other strains, was identified as a Pseudomonas stutzeri or its similar strain when analyzed by taxonomical charicteristics. Optimum pH and temperature for the growth of the P. stutzeri were 7.0 and 30℃ respectively. This strain was resistant to antibiotics, such as ampicillin and tetracycline, and heavy metals, such as lead and chromium. This strain utilized phenol benzen or toluene as a sole carbon source. The rate of copper accumulation in P. stutzeri cell was enhanced at low concentration of Cu in the growth media. The maximum copper absorption by this strain grown in 10ppm of Cu at 3 days after culture was 49%, but in 50ppm and 100ppm of Cu was 38% and 32%, respectively. The optimal temprature and pH for the effective intracellular accumulation of copper were 30℃ and initial pH 8.0 under shaking culture.

구리 耐性菌(Pseudomonas stutzeri)의 菌體內 구리 蓄積機作

許鍾秀,曺周植,韓文圭,李洪宰,林永成 경상대학교 환경보전연구소 1994 環境保全硏究所報 Vol.2 No.1

In order to study the mechanism of the copper accumulation in the cell of copper-tolerant bacterium, Pseudomonas stutzeri, distribution of the copper in the cell were investigated. Approximately 61% of the accumulated copper was distributed on the cell wall and the other 39% portion was in cytoplasm. 78% of the copper in the cell wall fractions was present in the polyphosphate and polysaccharide fractions, but most of copper in the cytoplasm fractions was in protein and nucleic acid. Copper affected the protein synthesis in P. stutzeri. The intracellular protein content was decreased by copper addition, but the soluble protein precipitated by ammonium sulfate(30-75% saturation) was increased as compared to that from the cells grown without copper. Furthermore, in the cells grown with copper, high-molecular-weight soluble protein was increased, compared with the cells grown without copper, but low-molecular -weight soluble protein was decreased. These results indicate that copper inhibited the intracellular protein biosynthesis but enhanced the biosynthesis of the high-molecular-weight soluble protein precipitated by ammonium sulfate(30-75% saturation).

EXTENDED KALMAN FILTER를 이용한 유도전동기 벡터 제어

서영수,허종명 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

This paper presents a detailed study of the extended Kalman filter(EKF) for estimating the rotor speed of an induction motor drive. The general structure of the Kalman filter is reviewed and the various system vectors and matrices are defined. By including the rotor speed as a state variable, the EKF equations are established from a discrete two axis model of the three-phase induction motor. using the software MATLAB/Simulink, simulation of the EKF speed estimation algorithm is carried out for an induction motor drive with direct self control. The investigations show that the EKF is capable of tracking the actual rotor speed provided that the elements of the covariance matrices are properly selected. Moreover, the performance of the EKF is satisfactory even in the presence of noise or when there are variations in the indution machine parameters.

분자유전학적방법에 의해 확진된 Leber씨 유전성 시신경병증 2예

김응규,김종환,김상진,예종수,김성은,허경,원인건,황정민 대한신경과학회 1998 대한신경과학회지 Vol.16 No.1

관동맥풍선시술중 혈전동반 병변에 대한 구조치료(Rescue Utilization)로서의 Abciximab(ReoPro^(�)의 효과

두영철,채경수,최종형,허필석,송관욱,홍경순,박대균,한규록,오동진,유규형,임종윤,고영박,이광학,이영 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.12

미역 폐기물의 중금속 흡탈착 특성

조주식,박일남,허종수,이영석 한국환경농학회 2004 한국환경농학회지 Vol.23 No.2

본 연구에서는 생물흡착제인 미역을 사용하여 인공폐액 내의 중금속의 흡착 특성을 조사하였으며, 다음과 같은 결론을 얻었다. 미역 폐기물을 이용하여 제조된 생물흡착제의 단일 중금속 흡착효율은 각 중금속 100 mg/L인 용액에 biosorbent 3 g을 처리했을 때 Pb는 거의 100%였고 Cu와 Cd가 약 85~86% Zn과 Cr은 약 60~64%, Co와 Ni은 약 57%, Mn은 약 48%였다. 복합중금속 흡착효율은 단일중금속 흡착효율에 비하여 Pb를 제외한 다른 중금속들은 크게 감소되었으나 biosorbent g당 전체 중금속 흡착량은 더 증가되었다. 온도, pH에 따른 중금속 흡착효율은 별 차이가 없었으며 전반적으로 pH는 pH 5~6 범위, 온도는 20~30℃ 범위의 실온이 적당한 것으로 나타났다. 생물흡착제의 흡착평형은 모든 중금속이 1시간 이내에 이루어졌다. Freundlich와 Langmuir 등온흡착식을 구하여 실제 폐수처리에 적용여부를 검토한 결과 Pb, Cu, Cr, Cd, Co 순으로 비교적 친화도가 큰 것으로 나타났다. Freundlich 등온흡착식에 가장 잘 일치하였다. 모든 중금속의 Freondlich 지수 1/n값이 0.1~0.5 범위에 포함되어 흡착이 용이하고, Pb, Cr, Cu, Cd 순으로 비교적 평형 흡착량이 큰 것으로 나타났으며, 이 결과는 Langmuir 흡착등온식의 결과와 거의 유사하였다. 따라서, 중금속 농도범위에 따른 한계범위를 설정하여 등온흡착식을 적용시키면 매우 높은 중금속 처리효율을 나타낼 것으로 판단된다. 생물흡착제가 중금속 흡착 후의 구조적인 특성을 조사하기 위하여 FT-IR 분석 결과, 중금속 이온과 치환될 것으로 생각되는 functional group을 확인할 수 있었으며, 3,400/cm에서의 -OH group, 1,648/cm에서의 C=O bond, 1,426/cm에서의 C-O bond 그리고 850/cm에서의 S=O등이 전반적으로 중금속 흡착 후에 peak가 커지는 것이 관찰되었다. 생물흡착제의 재사용 가능성을 검토하기 위하여 최적의 중금속 탈착조건 검토하였는데, 탈착제 종류에 따른 중금속 탈착효율은 탈착제 종류와 중금속 종류에 따라 차이가 있었고, 전반적으로 NTA>H₂SO₄>HCl>EDTA 순으로 우수하였으며, 전체적으로 특정 중금속의 탈착효율이 떨어지지 않는 NTA가 탈착제로 가장 적합하였다. NTA농도에 따른 중금속 탈착효율은 NTA 0.1~0.3% 농도범위에서는 농도 증가에 따라 탈착효율도 약간 증가되었으나 그 이상의 농도에서는 별 차이 없었다. 온도와 pH 변화에 따른 중금속 탈착효율은 Cr을 제외한 다른 중금속들은 크게 영향을 받지 않는 것으로 나타났으며 전반적으로 온도는 30℃, pH는 2에서 높았다. 탈착제 접촉 반응시간에 따른 탈착효율은 10분 이내에 전체 탈착량의 80%이상이 탈착 되었으며 1시간 이후에는 거의 변화가 없었다. The adsorption and desorption of Pb, Cd, Co, Zn, Cr, Co, Ni, and Mo on the waste Undaria sp. were studied. Except for Pb, the mono adsorption rate for all heavy metals were lower than that of the heavy metals mixed. However, the adsorption capacity of the heavy metals by 1 g of biosorbent in mixed heavy metals increased. According to FT-IR analysis of the biosorbent after heavy metal biosorption, the replacement of the functional group by the heavy metals ions could be confirmed and the inverted peaks became larger after heavy metals adsorption. The adsorption equilibrium of heavy metals was reached in about 1 hour. The equilibrium parameters were determined based on Langmuir and Freundlich isotherms. The affinity of metals on the biosorbent decreased in the following order: Pb>Cu>Cr>Cd>Co. The desorption rate decreased in the following sequence: NTA>H₂SO₄>HCl>EDTA. The desorption rate of heavy metals by NTA increased with increase in the concentration from 0.1 to 0.3% but the desorption rate became constant beyond 0.3%. Therefore, it represented that desorption rate of heavy metals was suitable under optimized condition (30℃, pH 2 and 0.3% NTA solution) and was fast with 80% or more the uptake occurring within 10 min of contact time.

단체행위모형에 관한 고찰

沈京燮,韓鍾洙,許鎭九 단국대학교 1992 論文集 Vol.26 No.-

We have developed a formal model of collective action which brings together features associated in particular with the work of Elster, Schelling and Akerlof. We would argue that the model is capable of application to a wide range of empirical contexts involving issues of collective action where the free-rider problem renders conventional economic analysis inadequate. The approach offers insights into the historical development of such groups as trade unions and could be empirically tested against such processes. As Hardin(1982) has shown the results obtained here can carry over from the issue of collective action to that of the multi-person prisoner's dilemma. A number of aspects of the model deserve further development. Here we indicate two such aspects. First, we have treated the ε^1 distribution as determined exogenously. Alternatively, we could follow Jones(1984) and make our ε^1 parameter endogenous within the model. One way of doint this would be to make ε^1 itself dependent upon the individual's decision with respect to membership of collective action. Or we could think of the individual as influenced by a vector of social norms with his/her attitude to each affected throughεi by his/her behavior with respect to the others. Second, we have abstracted from the economic structure or game in which the collective action is, or is not, taking place. Clearly, a complete model needs to specify the interactions between the economic parameters and social custom inuluences on collective action. We have tried this elsewhere for the issue of trade union membership and wage determination(see Naylor(1989)) but do not pursue this here as any particularisation is likely to be context-specific.

합천호 수질 및 영양단계에 대한 평가

최형섭,조인철,변종환,문병현,허종수 한국환경농학회 2003 한국환경농학회지 Vol.22 No.1

호수 유입 하천인 황강의 1996~2001년 년도별 평균 수질은 COD 3.1~4,2 mg/L, T-N 2.460~3.550 mg/L, T-P 0.111~0.201 mg/L이였다. 호수내의 평균 수질은 COD가 1996년 1.9 mg/L에서 2001년 2.7 mg/L로 매년 조금씩 종가하는 것으로 나타났다. 호수내의 1996~2001년 년도별 평균 T-N 농도는 1.393~l.792 mg/L 이었으며, T-P농도는 0.018~0.023 mg/L 이였다. 식물성 플랑크톤의 발생 정도를 나타내는 Chl-α와 T-N, T-P와의 상관계수는 각각 0,382, 0.372였으며, 또한 수온과 강우량에 대한 상관계수는 각각 0.589, 0,526이였다. 따라서 합천댐의 경우 식물성 플랑크톤의 발생이 T-N, T-P보다 수온 및 강수량과 더 밀접한 관계가 있는 것으로 나타났다. 그리고 호수의 영양단계는 중영양~부영양 단계에 있는 것으로 평가되었다. The yearly average water qualifies of the Whang river, which flows into the Hapcheon lake, were COD 3.1~4.2 mg/L, T-N 2.460~3.550 mg/L, and T-P 0.111~0.201 mg/L during 1996~2001. The yearly average COD concentration of Hapcheon lake was increased from 1.9 mg/L (in 1996) to 2.7 mg/L (in 2000). However, T-N and T-P concentration of Hapcheon lake did not show increasing trend over the 6 year period. During 1996~2001, the yearly average concentrations of T-N, T-P were 1.383?1.792 mg/L, 0.018~0.023 mg/L, respectively. The correlation coefficients between chlorophyll α and T-N, T-P, rainfall intensity, water temperature were 0.382, 0372, 0.589, and 0.526, respectively. Therefore, the rainfall and water temperature appeared to play an important role for the variations of chlorophyll α concentration in the Hapcheon lake. Trophic state of the Hapcheon lake were evaluated to be in the range of mesotrophic to eutrophic.

인의 흡착능 평가를 통한 인공습지 하수처리 시스템의 여재 선발

이홍재,서동철,조주식,허종수 한국환경농학회 2003 한국환경농학회지 Vol.22 No.2

인공습지 하수처리장이 축적되어 하수처리장 수명의 제한인자로 작용하는 인의 포화치를 조사하기 위해 여재 입경별 최대 인 흡착능을 조사하여 최적의 여재를 선정하였고, 또한 유기물과 굴패각의 인 흡착제로서 사용가능 여부를 조사하기 위해 최대 인 흡착능을 조사하였다. 그리고 인공습지 하수처리장에서 하수처리장의 수명을 연장하기 위한 방안을 검토하기 위해 여재별로 흡착제인 유기물 양을 달리하여 최대 인 흡착능을 조사하였고, 최적으로 선정된 여재에 Ca, Mg, Al, Fe 및 굴패각 첨가에 따른 최대 인 흡착능을 조사하였다. 여재 입경별 최대 인 흡착능을 조사한 결과 여재 입경이 작아질수록 최대 인 흡착능이 증가하는 경향으로 여재 대(4~10 mm), 중(2~4 mm) 및 소(0.1~2mm)의 최대 인 흡착능은 각각 8, 10 및 22mg/kg로서 여재 입경이 0.1~2 mm인 여재소(C)가 최적의 여재이었고, 유기물과 굴패각의 최대 인 흡착능을 조사한 결과 유기물이 1,000 mg/kg 및 굴패각이 833 mg/kg이었다. 입경별 여재에 유기물을 첨가하였을 경우 모든 여재에서 유기물 첨가량이 증가할수록 최대 인 흡착능이 점점 증가하였다. 따라서 인공습지 하수처리장에서 수초에 의해 쌓여 부식된 유기물은 인의 흡착능을 증가시켜 인 처리능력을 향상시킬 수 있을 것이라 사료되었다. 최적 여재인 여재 소(C)에 Ca, Mg, Al 및 Fe를 첨가하였을 경우 모든 조건에서 첨가량이 증가할수록 최대 인 흡착능도 점점 증가하였으며, 특히 Ca 0.1% 첨가시 인 흡착능이 885 mg/kg으로 급격히 증가하였다. 굴패각을 여재에 첨가하여 흡착능을 조사한 결과 굴패각을 2% 첨가시 인 흡착능이 약 22 mg/kg에서 약 36 mg/kg으로 약 14 mg/kg이 증가하였다. The phosphorus(P) adsorption capacities of various filter media were investigated in relation to the size and types of filter media to screen the optimum condition. The objective of this study was to evaluate the constructed wetland longevity by improving P adsorption capacity. The maximum P adsorption capacities of filter media A(4∼10 ㎜), B(2∼4 ㎜) and C(0.l∼2 ㎜) were 8, 10 and 22 ㎎/㎏, respectively, showing those increased as the filter media size decreased. Among the experimental media, the optimum filter media size was 0.1-2 ㎜. When the filter medium was supplemented with organic materials which were piled up and decayed in the constructed wetland, the P adsorption capacity was significantly enhanced. Under the conditions of optimum filter media size, the respective maximum P adsorption capacities of filter media C when supplemented with Ca, Mg, Al and Fe were higher than that of filter media C. However the addition of Ca, Mg, Al and Fe to constructed wetland were not recommended because of the possibility of their secondary pollution. The maximum P adsorption capacity of filter media C was 22 ㎎/㎏, but this was increased to 36 ㎎/㎏ when filter media C was supplemented with 2% oyster shell.

낙동강 상수원수의 오존처리 효과 : II. 회분식 오존처리에 의한 휘발성유기화합물 및 조류제거 효과 II. Removal of VOCs and Algae in Raw Water by Conducting Batch Test of Ozonation Experiments

임영식,이홍재,이도진,허종수,손보균,조주식 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

This study was carried out to evaluate the pollutant removal efficiencies of the advanced drinking water treatment using ozonation process. For raw water, Nakdong River was used. By conducting batch test of ozonation efficiencies of the ozonation were 94 to 92%, respectively. Removal efficiencies of single VOC compound or mixed VOC compounds in the raw water were 80% to 90% by the ozonation with 2 mg/ℓ dosage and 10 minutes contact time. Removal efficiencies of ABS by the ozonation with 1 mg/ℓ, 3 mg/ℓ dosage and 20 minutes contact time were 83% to 96%, respectively. Almost 67% of chlorophyll-a at the concentration of 38.㎍/ℓ was removed by ozonation at ozone dosage of 1mg/ℓ for 20 min. considering the efficiency of ozone utilization and water treatment, the most effective ozonation could be obtained with high ozone dosage and short and short contact time.