Overexpression of alfalfa <i>Orange</i> gene in tobacco enhances carotenoid accumulation and tolerance to multiple abiotic stresses

Wang, Zhi,Xu, Weizhou,Kang, Jiyue,Li, Min,Huang, Jin,Ke, Qingbo,Kim, Ho Soo,Xu, Bingcheng,Kwak, Sang-Soo Elsevier 2018 Vol. No.

<P><B>Abstract</B></P> <P>The multifunctional Orange (Or) protein plays crucial roles in carotenoid homeostasis, photosynthesis stabilization, and antioxidant activity in plants under various abiotic stress conditions. The <I>Or</I> gene has been cloned in several crops but not in alfalfa (<I>Medicago sativa</I> L.). Alfalfa is widely cultivated across the world; however, its cultivation is largely limited by various abiotic stresses, including drought. In this study, we isolated the <I>Or</I> gene from alfalfa (<I>MsOr</I>) cv. Xinjiang Daye. The amino acid sequence of the deduced MsOr protein revealed that the protein contained two trans-membrane domains and a DnaJ cysteine-rich zinc finger domain, and showed a high level of similarity with the Or protein of other plants species. The MsOr protein was localized in leaf chloroplasts of tobacco. The expression of <I>MsOr</I> was the highest in mature leaves and was significantly induced by abiotic stresses, especially drought. To perform functional analysis of the <I>MsOr</I> gene, we overexpressed <I>MsOr</I> gene in tobacco (<I>Nicotiana benthamiana</I>). Compared with wild-type (WT) plants, transgenic tobacco lines showed higher carotenoid accumulation and increased tolerance to various abiotic stresses, including drought, heat, salt, and methyl viologen-mediated oxidative stress. Additionally, contents of hydrogen peroxide and malondialdehyde were lower in the transgenic lines than in WT plants, suggesting superior membrane stability and antioxidant capacity of TOR lines under multiple abiotic stresses. These results indicate the <I>MsOr</I> gene as a potential target for the development of alfalfa cultivars with enhanced carotenoid content and tolerance to multiple environmental stresses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Orange (<I>MsOr</I>) gene from alfalfa was isolated and characterized in transgenic tobacco. </LI> <LI> <I>MsOr</I> gene was localized to chloroplasts and strongly induced by abiotic stresses including drought. </LI> <LI> <I>MsOr</I> expressing tobacco plants showed enhanced tolerance to drought, heat, salt and oxidative stress. </LI> </UL> </P>

MPEG-phenylboronic acid modified doxorubicin as the efficient pathological pH-responsive nanoplatform for potential anti-cancer delivery

Jiyu Li,Li Zhu,Shuai Wu,Guixue Wang,Yidan Chen,Wei Wu 한국고분자학회 2023 Macromolecular Research Vol.31 No.3

Chemotherapy is the main strategy for inhibiting the tumor growth. However, during the therapy, the side effect of anti-cancer drug will injure normal cells. For reducing the toxic of the free drug, stimuli responsive nanoparticles (NPs) have been prepared base on the pathological acidic environment around tumor for safe and efficient anti-tumor applications. In this study, we fabricated the phenylboronic acid (PBA) modified monomethoxy polyethylene glycol (MEPG) for encapsulating doxorubicin (DOX NPs). In the drug delivery system (DDS), the pH-responsive boric ester could be stable during drug delivery, and enhance DOX rapidly release in tumor lesion under the acidic stimuli. In vitro studies further demonstrated that DOX NPs could efficiently endocytose by tumor cells, and exhibit the similar anti-tumor activity as the free DOX resulting from the pH-responsive drug release profiles under tumor acidic stimuli. In summary, DOX NPs could be a feasible candidate to improve the safe and efficient DOX delivery for improving the potential anti-tumor applications.

Durability of CFRP Strengthened Steel Plates Under Wet and Dry Cycles

Shan Li,Jiyue Hu,Yi-Yan Lu,Hong-Jun Liang 한국강구조학회 2018 International Journal of Steel Structures Vol.18 No.2

This paper presents tensile test results of CFRP strengthened steel plates (CS) after wet and dry corrosion. The number of cycle times and strengthened schemes were investigated. The results showed that the CS specimens, subjected to wet–dry corrosion, behaved in a more brittle manner and appeared massive CFRP and adhesive rupturing coincided with the failure. As the wet–dry cycle increasing, the yield strength and ultimate strength of CS strengthened specimens decreased signifi - cantly, while the stiff ness before and beyond yield strength degraded slightly. Furthermore, the yield strength and ultimate strength of double-sided specimens subjected to wet–dry cycles were higher than those of one-sided specimens. Finally, analytical formulas considering wet–dry corrosion were proposed to predict elastic modulus, yield strength, tangent modulus, ultimate strength of CS specimens subjected to wet–dry cycles, respectively. The calculated results were in good agreement with experimental values.

Holistic Life-cycle Cost-benefit Analysis of Green Buildings: A China Case Study

Xiaojuan Li,Mingchao Lin,Wanjun Xie,Chi Yung Jim,Jiyu Lai,Liping Cheng 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.11

Green buildings, compared with conventional ones, can mitigate the rapid growth of China's carbon emissions. They offer many advantages, such as saving resources and reducing negative environmental impacts. However, few studies have assessed their incremental costs and social benefits. This study comprehensively analyzed the incremental costs and benefits of green buildings in China through the life cycle stages of preparation, design, construction, and operation-management. The Analytic Hierarchy Process and Matter Element Analysis were jointly applied to establish a comprehensive green-building cost-benefit evaluation system. Based on expert judgment, an embracive two-level index scheme and a three-step grading strategy were developed. A residential case study tested the applicability of the model. It yielded the largest share of incremental cost in the construction phase at 90.5%. The economic benefits accounted for the largest proportion of incremental benefits at 72.22%, meeting the green building criteria and achieving the highest 3-star rating. The study has developed a full life-cycle costing methodology for green building design and quantified the benefits at each stage. The findings offer the theoretical and practical basis to refine the assessment of green building projects and improve green building performance to meet sustainable development goals.

Effects of Florfenicol and Chromium (III) on Humoral Immune Response in Chicks

Cao, Jiyue,Li, Kui,Lu, Xiaocong,Zhao, Yaxin Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.3

One hundred and sixty day-old Hainan chicks were randomly allotted into eight pens to investigate the effect of different dietary concentrations of chromium (Cr) in the form of chromium chloride, and different dosages of florfenicol on humoral immune responses by determining antibody titers to Newcastle disease (ND) vaccines using the hemagglutination inhibition test. The results indicated that ND antibody titers were significantly higher in chicks receiving Cr at low (5 mg/kg feed) and middle (10 mg/kg feed) dose compared with the control (p<0.01). However, ND antibody titers were significantly decreased in chicks receiving Cr at a high dosage of 500 mg/kg feed (p<0.01), though the ND antibody titers of the early days (d 21 and d 28 of age) were higher than that of the control group. It is suggested that excessive Cr intake has detrimental effects on ND antibody production in chicks. No significantly lower response was measured in chicks that received florfenicol at a low dosage of 50 mg/kg feed (p>0.05), but the ND antibody titers were significantly decreased in chicks receiving 200 and 400 mg/kg feed of the drug (p<0.01). The ND antibody titers of group receiving 200 mg/kg feed of florfenicol plus 10 mg/kg Cr were slightly higher than that of the group receiving single florfenicol of 200 mg/kg although, no significant differences were observed between these two treatments. It is suggested that the humoral immune response impaired by florfenicol (200 mg/kg feed) could not be significantly reversed by Cr (10 mg/kg feed).

Low Complexity Discrete Hartley Transform Precoded OFDM System over Frequency-Selective Fading Channel

Xing Ouyang,Jiyu Jin,Guiyue Jin,Peng Li 한국전자통신연구원 2015 ETRI Journal Vol.37 No.1

Orthogonal frequency-division multiplexing (OFDM) suffers from spectral nulls of frequency-selective fading channels. Linear precoded (LP-) OFDM is an effective method that guarantees symbol detectability by spreading the frequency-domain symbols over the whole spectrum. This paper proposes a computationally efficient and low-cost implementation for discrete Hartley transform (DHT) precoded OFDM systems. Compared to conventional DHT-OFDM systems, at the transmitter, both the DHT and the inverse discrete Fourier transform are replaced by a one-level butterfly structure that involves only one addition per symbol to generate the time-domain DHT-OFDM signal. At the receiver, only the DHT is required to recover the distorted signal with a single-tap equalizer in contrast to both the DHT and the DFT in the conventional DHT-OFDM. Theoretical analysis of DHT-OFDM with linear equalizers is presented and confirmed by numerical simulation. It is shown that the proposed DHT-OFDM system achieves similar performance when compared to other LP-OFDMs but exhibits a lower implementation complexity and peak-to-average power ratio.

Measurement of Porosity by EPMA-EDS Image Processing

Hung, Minhui,Li, Xiangting,Xia, Jiyu,Ding, Chuanxian The Korean Vacuum Society 1997 Applied Science and Convergence Technology Vol.6 No.1

Porosity is one important characteristic feature and structural index of sprayed coatings. A method of measurement of porosity, EPMA-EDS image processing is developed in the paper. The characteristics of pores can be determined by processing of the image obtained from an electron microscope via VISTA, Not only the porosity can be presented but also the statistical result of pore size distribution. Finally it can be drawn from this paper that EPMA-EDS is a quite effective method to completely characterize the pores in plasma sprayed coatings.

Molecular phylogenetic studies on clinical bovine piroplasmosis caused by benign Theileria in Shaanxi Province, China

Jing Wang,Jiyu Zhang,Zhen Zhu,Xuzheng Zhou,Bing Li 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.6

A group of benign Theileria species, which are often referred to as T. orientalis/T. buffeli/T. sergenti group, has low pathogenicity in cattle. Herein, we report on Theileria spp. in cattle on a farm from China. Based on phylogenetic analysis of the major piroplasm surface protein gene sequences, we detected 6 genotypes that were categorized as Types 1, 2, 3, 4, and 5 as well as an additional Type 9 genotype. The new epidemiological features of the T. orientalis/T. buffeli/T. sergenti parasites in China indicate a greater diversity in the genetics of these species than had been previously thought.

Bavachin Suppresses Alpha-Hemolysin Expression and Protects Mice from Pneumonia Infection by Staphylococcus aureus

( Ye Tao ),( Dazhong Sun ),( Xinran Ren ),( Yicheng Zhao ),( Hengjian Zhang ),( Tao Jiang ),( Jiyu Guan ),( Yong Tang ),( Wu Song ),( Shuqiang Li ),( Li Wang ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10

Staphylococcus aureus (S. aureus) infection causes dramatic harm to human health as well as to livestock development. As an important virulence factor, alpha-hemolysin (hla) is critical in the process of S. aureus infection. In this report, we found that bavachin, a natural flavonoid, not only efficiently inhibited the hemolytic activity of hla, but was also capable of inhibiting it on transcriptional and translational levels. Moreover, further data revealed that bavachin had no neutralizing activity on hla, which did not affect the formation of hla heptamers and exhibited no effects on the hla thermal stability. In vitro assays showed that bavachin was able to reduce the S. aureus-induced damage of A549 cells. Thus, bavachin repressed the lethality of pneumonia infection, lung bacterial load and lung tissue inflammation in mice, providing potent protection to mice models in vivo. Our results indicated that bavachin has the potential for development as a candidate hla inhibitor against S. aureus.