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Simple sequence repeat marker development from Codonopsis lanceolata and genetic relation analysis
Kim, Serim,Jeong, Ji Hee,Chung, Hee,Kim, Ji Hyeon,Gil, Jinsu,Yoo, Jemin,Um, Yurry,Kim, Ok Tae,Kim, Tae Dong,Kim, Yong-Yul,Lee, Dong Hoon,Kim, Ho Bang,Lee, Yi The Korean Society of Plant Biotechnology 2016 식물생명공학회지 Vol.43 No.2
In this study, we developed 15 novel polymorphic simple sequence repeat (SSR) markers by SSR-enriched genomic library construction from Codonopsis lanceolata. We obtained a total of 226 non-redundant contig sequences from the assembly process and designed primer sets. These markers were applied to 53 accessions representing the cultivated C. lanceolata in South Korea. Fifteen markers were sufficiently polymorphic, and were used to analyze the genetic relationships between the cultivated C. lanceolata. One hundred three alleles of the 15 SSR markers ranged from 3 to 19 alleles at each locus, with an average of 6.87. By cluster analysis, we detected clear genetic differences in most of the accessions, with genetic distance varying from 0.73 to 0.93. Phylogenic analysis indicated that the accessions that were collected from the same area were distributed evenly in the phylogenetic tree. These results indicate that there is no correlative genetic relationship between geographic areas. These markers will be useful in differentiating C. lanceolata genetic resources and in selecting suitable lines for a systemic breeding program.
Analysis of genetic diversity of Codonopsis lanceolata cultivated in Korea using SSR makers
Serim Kim,Ji Hee Jeong,Jinsu Gil,Tae Dong Kim,Yurry Um,Ok Tae Kim,Ho Bang Kim,Hee Chung,Yi Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Codonopsis lanceolata is a perennial climber. The roots are used as medicinal materials or vegetables. Recently, demand for C. lanceolata is increasing as a healthy food. C. lanceolata is distributed in India and East Asia such as China, Japan as well as Korea. In South Korea, this plant is widely cultivated in Gangwon-do province. No C. lanceolata varieties were developed in Korea. The objective of this study is to analyze genetic diversity of C. lanceolata cultivated in Korea using SSR makers. C. lanceolata roots were collected in each region were cultivated in Chungbuk National University greenhouse. Samples were obtained from fresh leaves of 5 plants from each collection region. The genomic DNA was extracted using CTAB. Genetic diversity was analysed using 4 sets of C. lanceolata SSR makers. PCR was performed in total 20 μL reaction volume containing 20 ng of DNA template, 5 pmole of primers. The genotypes of the analyzed samples were very similar. That means that the genetic diversity of C. lanceolata cultivated in Korea is very low.
Genetic diversity analysis of wild Codonopsis lanceolata in Korea using SSR makers
Serim Kim,Ji Hee Jeong,Jinsu Gil,Tae Dong Kim,Yurry Um,Ok Tae Kim,Ho Bang Kim,Yi Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
In this study, genetic diversity of wild Codonopsis lanceolata collected in Korea were analysed using SSR makers. Wild C. lanceolata roots were collected in Jeollanam-do Jangheung-gun Choentae Mountain as in roots. The wild C. lanceolata plants were cultivated in Chungbuk National University greenhouse and the leaves were sampled from 36 plants. The genomic DNA of C. lanceolata was extracted using CTAB. PCR was performed using a program of 35 cycles at 94℃ for 30 sec, 60℃ for 30 sec, and 72℃ for 30 sec with an pre-denaturation of 94℃ for 5 min and a final extension of 72℃ for 30 min. The PCR reaction mixture contains 5 pmole of primers and 20 ng of DNA template in a 20 μL reaction volume. The genotype of the analyzed samples were very different. Therefore, the wild C. lanceolata collected in Korea look genetically diverse.
Analysis of Phylogenetic Relationship from Angelica gigas collected in Korea using RAPD Markers
Jinsu Gil,Serim Kim,Yurry Um,Ok Tae Kim,Hee Chung,Seon-Woo Cha,Yi Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Angelica gigas, also called Dang Gui or Korean Angelica, is a major medicinal herb used in Asian countries such as Korea, Japan and China. In Korea, we are using the roots of A. gigas., but, they are using Angelica sinensis in China and using Angelica acutiloba. in Japan to obtain many active constituents such as dercursin, decursinol angelate, nodakenetin, nodakenin, umbelliferone, β-sisterol, or α-pinene. The plants of the Angelica family are used to improve gynecological health. The biggest problem in the cultivation of A. gigas is bolting. If the bolting occurs, A. gigas can not be used as a medicinal component because the roots are lignified. In this study, 11 A. gigas genetic resources in Korea; 1. Hwangje variety, 2. Sungwoo Jongmyo company, 3. Bonghwa No. 1, 4. Bonghwa No. 2, 5. Bonghwa No. 3, 6. Bonghwa No. 4, 7. Jechun local variety, 8. Jirisan local variety, 9. Manchu variety in Eumseong, 10. Manchu variety in Bonghwa, 11. Jinbu local variety, were collected and performed phylogenetic analysis using RAPD molecular markers.
Yoon, Jinsu,Han, Jungmin,Choi, Bongsik,Lee, Yongwoo,Kim, Yeamin,Park, Jinhee,Lim, Meehyun,Kang, Min-Ho,Kim, Dae Hwan,Kim, Dong Myong,Kim, Sungho,Choi, Sung-Jin American Chemical Society 2018 ACS NANO Vol.12 No.6
<P>Electronics that degrade after stable operation for a desired operating time, called transient electronics, are of great interest in many fields, including biomedical implants, secure memory devices, and environmental sensors. Thus, the development of transient materials is critical for the advancement of transient electronics and their applications. However, previous reports have mostly relied on achieving transience in aqueous solutions, where the transience time is largely predetermined based on the materials initially selected at the beginning of the fabrication. Therefore, accurate control of the transience time is difficult, thereby limiting their application. In this work, we demonstrate transient electronics based on a water-soluble poly(vinyl alcohol) (PVA) substrate on which carbon nanotube (CNT)-based field-effect transistors were fabricated. We regulated the structural parameters of the PVA substrate using a three-dimensional (3D) printer to accurately control and program the transience time of the PVA substrate in water. The 3D printing technology can produce complex objects directly, thus enabling the efficient fabrication of a transient substrate with a prescribed and controlled transience time. In addition, the 3D printer was used to develop a facile method for the selective and partial destruction of electronics.</P> [FIG OMISSION]</BR>
생활 속 미세먼지 영향평가를 위한 소형센서의 신뢰성 및 활용성 평가
김진수 ( Jinsu Kim ),장유정 ( Youjung Jang ),김진석 ( Jinseok Kim ),박민우 ( Minwoo Park ),부찬종 ( Chanjong Bu ),이윤구 ( Yungu Lee ),김윤하 ( Younha Kim ),우정헌 ( Jung-hun Woo ) 한국환경영향평가학회 2018 환경영향평가 Vol.27 No.4
국내의 미세먼지 문제가 심각해짐에 따라 개인이 활동하는 주변공간의 미세먼지 농도를 알고자 하는 욕구 또한 증가하여 직접 미세먼지의 실시간 농도를 측정할 수 있는 휴대용 미세먼지측정센서에 대한 수요가 증가하고 있다. 그러나 시중에 판매되는 미세먼지 간이측정기는 정해진 인증기준 없이 제작·판매되고 있다. 본 연구에서는 최근 판매가 급증하고 있는 휴대용 미세먼지 측정센서의 농도값을 어느 정도 신뢰할 수 있는지와 이러한 센서들이 어떠한 경우에 활용될 수 있는 지에 대해 일반적인 시민의 입장에서 고찰할 필요가 있다고 판단하였다. 이를 위해, 1) 기기 간 상호비교 및 보다 정확한 장비와의 비교를 수행하고, 2) 휴대용 기기를 활용하여 미세먼지의 영향을 저감할 수 있는 방안들에 대한 검증실험을 수행해 보았다. 그 결과 휴대용센서들의 농도 절대값을 그대로 신뢰하기엔 문제가 있었지만, 그 재현성과 선형성은 실생활에서 활용되기에 유용하다고 판단되었다. 또한 휴대용 미세먼지 측정센서를 사용함으로써 사용자들은 자기 주변의 변화하는 미세먼지 농도에 대해 빠르게 인지하고 대처 할 수 있을 것으로 판단되어, 미세 먼지 오염의 피해를 줄이는 데 활용할 수 있을 것이라 기대된다. As atmospheric fine dust problems in Korea become more serious, there are growing needs to find the concentration of fine particles in indoor and outdoor areas and there are increasing demands for sensor-based portable monitoring devices capable of measuring fine dust concentrations instantly. The low-cost portable monitoring devices have been widely manufactured and used without the prescribed certification standards which would cause unnecessary confusion to the concerned public. To evaluate the reliability those devices and to improve their usability, following studies were conducted in this work; 1) The comparisons between sensor-based devices and comparison with more accurate devices were performed. 2) Several experiments were conducted to understand usefulness of the portable monitoring devices. As results, the absolute concentration levels need to be adjusted due to insensitivity of the tiny light scattering sensors in the portable devices, but their linearity and reproducibility seem to be acceptable. By using those monitoring devices, users are expected to have benefits of recognizing the changes of concentration more quickly and could help preventing themselves from the adverse health impacts.