요 배양검사에서 양성인 어린이의 임상적 분석

최두영,김재일,김동적 圓光大學校 醫科學硏究所 1997 圓光醫科學 Vol.13 No.1-2

A clinical analysis was done for the 110 pediatric patients with positive urine culture during Jun. 1992 to Dec. 1995 retrospectively. The patients who were hospitalized were classified group Ⅰ, under 29 days of age, and group Ⅱ, number of 29 days of age to 15 years old, respectively. The diagnostic criteria of urinary tract infection were first, the numbers of colonies of single organism isolated from clean urine collected by catheter was over 100,000/㎖. second, the numbers of colonies of single organism isolated from by supurapubic aspiration was over 1,000/㎖. third, two or more cultures yielding more than 100,000 colonies of a single identical organism from obtained by urine bag after aseptic preparation of genitalia. The results are summarized as follows : 1. The mean age of group Ⅰ was 9 days and 2.7 years in group Ⅱ. 2. Male to female ratio of group Ⅰ was 2.4 : 1 and 1.7 :1 in group Ⅱ. 3. The most common symptom was fever in both group Ⅰ(37%) and group Ⅱ(62%). 4. The pyuria(>5WBC/hpf) was detected in 21 cases(51%) of group Ⅰ and 32 cases(46%) of group Ⅱ. 5. The bacteriuria was detected in 22 cases(55%) of group Ⅰ and 25 cases(36%) in group Ⅱ. 6. The most commonly cultured bacterial species were E. coli in both group. 7. The most sensitive antibiotics for cultured E.coli was amikin.

Heterologous expression of two Physcomitrella patens group 3 late embryogenesis abundant protein (LEA3) genes confers salinity tolerance in arabidopsis

Jing Du,Lei Wang,Xiaochen Zhang,Xuan Xiao,Fang Wang,Pingliang Lin,Fang Bao,Yong Hu,Yi-Kun He 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

Salinity stress is a major limiting factor in agriculture and adversely affecting the whole plant. As a halophyte, the moss Physcomitrella patens, has been suggested to be an ideal model plant to study salinity tolerance and adaption. Two abiotic stress-responsive Group 3 Late Embryogenesis Abundant protein genes had been identified in P. patens and named as PpLEA3-1 and PpLEA3-2, respectively. Functions of these two genes were analyzed by heterologous expressions in Arabidopsis, driven either by their native P. patens promoters or by the 35S CaMV constitutive promoter. Phenotype analysis revealed that pLEA3::LEA3, pLEA3::LEA3::GFP and 35S::LEA3::GFP transgenic lines had stronger salinity resistance than that in the wild type and empty-vector control. Further analysis showed that the contents of proline and soluble sugar were increased and the malondialdehyde (MDA) were repressed in these transgenic plants after exposure to salinity stress. Our observations indicate that these two Group 3 PpLEA genes played a role in the adaption to salinity stress.

A PRODUCT FORMULA FOR LOCALIZATION OPERATORS

Du, Jing-De,Wong, M.M. Korean Mathematical Society 2000 대한수학회보 Vol.37 No.1

The product of two localization operators with symbols F and G in some subspace of $L^2(C^n)$ is shown to be a localization operator with symbol in $L^2(C^n)$ and a formula for the symbol of the product in terms of F and G is given.

Transcriptome profiling identifies immune response genes against porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in the lungs of piglets

Jing Zhang,Jing Wang,Xiong Zhang,Chunping Zhao,Sixuan Zhou,Chunlin Du,Ya Tan,Yu Zhang,Kaizhi Shi 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1

Background: Co-infections of the porcine reproductive and respiratory syndrome virus (PRRSV) and the Haemophilus parasuis (HPS) are severe in Chinese pigs, but the immune response genes against co-infected with 2 pathogens in the lungs have not been reported. Objectives: To understand the effect of PRRSV and/or HPS infection on the genes expression associated with lung immune function. Methods: The expression of the immune-related genes was analyzed using RNA-sequencing and bioinformatics. Differentially expressed genes (DEGs) were detected and identified by quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and western blotting assays. Results: All experimental pigs showed clinical symptoms and lung lesions. RNA-seq analysis showed that 922 DEGs in co-challenged pigs were more than in the HPS group (709 DEGs) and the PRRSV group (676 DEGs). Eleven DEGs validated by qRT-PCR were consistent with the RNA sequencing results. Eleven common Kyoto Encyclopedia of Genes and Genomes pathways related to infection and immune were found in single-infected and co-challenged pigs, including autophagy, cytokine-cytokine receptor interaction, and antigen processing and presentation, involving different DEGs. A model of immune response to infection with PRRSV and HPS was predicted among the DEGs in the co-challenged pigs. Dual oxidase 1 (DUOX1) and interleukin-21 (IL21) were detected by IHC and western blot and showed significant differences between the co-challenged pigs and the controls. Conclusions: These findings elucidated the transcriptome changes in the lungs after PRRSV and/or HPS infections, providing ideas for further study to inhibit ROS production and promote pulmonary fibrosis caused by co-challenging with PRRSV and HPS.

Associations of Single Nucleotide Polymorphisms in miR-146a, miR-196a, miR-149 and miR-499 with Colorectal Cancer Susceptibility

Du, Wei,Ma, Xue-Lei,Zhao, Chong,Liu, Tao,Du, Yu-Liang,Kong, Wei-Qi,Wei, Ben-Ling,Yu, Jia-Yun,Li, Yan-Yan,Huang, Jing-Wen,Li, Zi-Kang,Liu, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Background: MicroRNAs (miRNAs) are an abundant class of endogenous small non-coding RNAs of 20-25 nucleotides in length that function as negative gene regulators. MiRNAs play roles in most biological processes, as well as diverse human diseases including cancer. Recently, many studies investigated the association between SNPs in miR-146a rs2910164, miR-196a2 rs11614913, miR-149 rs229283, miR-499 rs3746444 and colorectal cancer (CRC), which results have been inconclusive. Methodology/Principal Findings: PubMed, EMBASE, CNKI databases were searched with the last search updated on November 5, 2013. For miR-196a2 rs11614913, a significantly decreased risk of CRC development was observed under three genetic models (dominant model: OR = 0.848, 95%CI: 0.735-0.979, P = 0.025; recessive model: OR = 0.838, 95%CI: 0.721-0.974, P = 0.021; homozygous model: OR = 0.754, 95%CI: 0.627-0.907, P = 0.003). In the subgroup analyses, miR-$196a2^*T$ variant was associated with a significantly decreased susceptibility of CRC (allele model: OR = 0.839, 95%CI: 0.749-0.940, P = 0.000; dominant model: OR = 0.770, 95%CI: 0.653-0.980, P = 0.002; recessive model: OR = 0.802, 95%CI: 0.685-0.939, P = 0.006; homozygous model: OR = 0.695, 95%CI: 0.570-0.847, P = 0.000). As for miR-149 rs2292832, the two genetic models (recessive model: OR = 1.199, 95% CI 1.028-1.398, P = 0.021; heterozygous model: OR = 1.226, 95% CI 1.039-1.447, P = 0.013) demonstrated increased susceptibility to CRC. On subgroup analysis, significantly increased susceptibility of CRC was found in the genetic models (recessive model: OR = 1.180, 95% CI 1.008-1.382, P = 0.040; heterozygous model: OR = 1.202, 95% CI 1.013-1.425, P = 0.013) in the Asian group. Conclusions: These findings supported that the miR-196a2 rs11614913 and miR-149 rs2292832 polymorphisms may contribute to susceptibility to CRC.

체험경제이론(EET)과 기술수용모델(TAM)을 활용한 메타버스 문화관광 사용자의 사용 의도에 관한 연구

두정(Du, Jing),장몽택(Zhang, Mengze) 한국문화산업학회 2023 문화산업연구 Vol.23 No.4

자기주도식 ‘소리 내어 읽기’가 한국어 듣기 능력에 미치는 영향 - KFL 중국인 학습자를 중심으로 -

두정 ( Du Jing ) 숙명여자대학교 한국어문화연구소 2019 한국어와 문화 Vol.25 No.-

본 연구에서는 주로 자기주도식 ‘소리 내어 읽기’ 방법이 KFL 중국인 학습자들의 한국어 듣기 능력 향상에 미치는 영향을 고찰하는 데에 목적이 있다. 이에 본 연구는 중국 대학교 한국어학과 3학년 대학생 총 60명을 대상으로 실험을 실시하였다. 이를 위해 본고는 학습자의 흥미를 고려한 ‘TV동화-행복한 세상’의 내용을 실험 자료로 사용하여 실험집단 학생에게 제공된 듣기 파일들을 듣고 문제를 풀어본 후 정답 선택 여부와 관계없이 자기주도식 ‘소리 내어 읽기’ 연습을 시켰다. 통제집단 학생들에게 매주마다 실험집단과 같은 실험 내용을 제공하며, 듣기 파일을 듣고 정답 선택 여부만 확인하는 문제를 풀어보는 듣기 연습을 시키도록 하였다. 이러한 실험을 실시하여 자기주도식 ‘소리 내어 읽기’ 방법이 중국 내 한국어학과 대학생의 듣기 능력 향상에 어떠한 영향을 미치는가를 분석하였다. 연구 결과에서 자기주도식 ‘소리 내어 읽기’ 방법은 KFL 중국인 학습자의 한국어 듣기 능력 향상에 유의미한 영향을 미치는 것을 확인하였다. 또한, 듣기 능력 상위자보다 하위자에게 듣기 능력의 향상에 더 효과적인 것을 밝히었다. 이와 같은 결과를 통해 자기주도식 ‘소리 내어 읽기’는 주로 원어민 교사의 교수를 받을 기회가 적은 KFL 학습자에게 수업시간 외의 효과적인 듣기 학습 방법으로 제언한다. 그리고 자기주도식 ‘소리 내어 읽기’ 방법은 한국어 학습자 중에 특히 듣기 하위자가 한국어 듣기 학습할 때 인지적 능력의 훈련에서 적극적으로 적용하는 것을 제언한다. The main purpose of this study is to investigate the learning effects on listening comprehension by ‘Self-oral reading’ practice. For this purpose, KFL Chinese learners were the research subjects. three hypotheses are set up under the assumption that there will be a difference in the result of listening comprehension through ‘Self-oral reading’ practice. The analysis results confirmed that the ‘Self-oral reading’ method has a significant impact on improving the learner's Korean listening comprehension. In addition, studies have shown that the learners with low Korean listening level in this method are more effective in improving their listening comprehension than those with higher listening levels. And the ‘Self-oral reading’ method is effective in improving the ability to “grasping the logic” and “understanding the attitude of the speaker”. In particular, the learners with lower levels of listening are more effective in ‘mastering the overall content’, ‘understanding reasons, basis, purpose’, and ‘understanding the attitude and mood of the speaker’.

한자어 부사의 형태론적 패턴에 대한 고찰

두청(DU, JING) 강원대학교 인문과학연구소 2018 인문과학연구 Vol.0 No.56

국어 형태론적 패턴에는 형태소가 순차적으로 배열되는 연쇄적 패턴과 형태소가 순차적으로 배열되지 않는 비연쇄적 패턴이 있다. 이 논문은 형태소가 순차적으로 배열하는 한자어 부사의 형성 과정을 분석하고, 연쇄적 패턴과 비연쇄적 패턴에 해당하는 것을 유형화하고자 한다. 패턴 유형별로 분류하면 한자어 부사의 연쇄적 패턴은 파생과 합성으로, 비연쇄적 패턴은 전환, 중첩, 혼종어로 구성된다. 파생 한자어 부사는 주로 2음절 이상의 어기와 결합하는 단어를 중심으로 접사의 판별기준에 따라 접두사와 접미사 설정에 대해 논의하였다. 합성 한자어 부사의 유형은 크게 직접 구성 요소의 자립성 유무와 직접 구성 요소의 관계로 나누고 분석하였다. 전환은 어기의 형식이 같은 채 접사를 결합하지 않고 품사의 성질을 바꾸는 패턴이다. ‘명사-부사’의 통용과 ‘수사-관형사-명사-부사’의 통용이 있다. 중첩은 의미를 강화하는 역할을 한다. ‘ABAB형’ 중첩, ‘AA형’ 중첩, ‘ABB형’ 중첩이 있다. 혼종어는 추가 유형으로 단어틀을 통해 서로 다른 어종의 언어 요소 결합을 논의하였다. The Korean morphological pattern has a cascade pattern in which morphemes are sequentially arranged and a non-cascade pattern in which morphemes are not sequentially arranged. In this paper, analyzes the formation process of adverbs arranged sequentially by morphemes, and try to typify those corresponding to cascade and non-cascade patterns. When classified by pattern type, the cascade pattern of Sino-Korean adverbs consists of derivation and synthesis, and the nonconsecutive pattern consists of conversion, reduplication, and hybrid word. The derivation Sino-Korean adverbs mainly discussed the prefix and suffix setting according to the criterion of the affix around the word which is combined with more than two syllable term. The types of synthetic Sino-Korean adverbs are divided into the relationship between direct component independence and direct component. Conversion is a pattern that changes the properties of parts of speech without combining affixes of the same form. There is a common use of ‘noun - adverb’ and ‘rhetorical - idiomatic - noun - adverb’. Reduplication plays a role in reinforcing meaning. There is the ‘ABAB-type’ reduplication, ‘AA-type’ reduplication and ‘ABB type’ reduplication. Hybrid word was discussed as an additional type, combining the elements of different fish species through word frames.

Naturally Occurring Lactic Acid Bacteria Isolated from Tomato Pomace Silage

Wu, Jing-Jing,Du, Rui-Ping,Gao, Min,Sui, Yao-Qiang,Xiu, Lei,Wang, Xiao Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.5

Silage making has become a significant method of forage conservation worldwide. To determine how tomato pomace (TP) may be used effectively as animal feed, it was ensilaged for 90 days and microbiology counts, fermentation characteristics and chemical composition of tomato pomace silage (TPS) were evaluated at the 30th, 60th, and 90th days, respectively. In addition, 103 lactic acid bacteria were isolated from TPS. Based on the phenotypic and chemotaxonomic characteristics, 16S rDNA sequence and carbohydrate fermentation tests, the isolates were identified as 17 species namely: Lactobacillus coryniformis subsp. torquens (0.97%), Lactobacillus pontis (0.97%), Lactobacillus hilgardii (0.97%), Lactobacillus pantheris (0.97%), Lactobacillus amylovorus (1.9%), Lactobacillus panis (1.9%), Lactobacillus vaginalis (1.9%), Lactobacillus rapi (1.9%), Lactobacillus buchneri (2.9%), Lactobacillus parafarraginis (2.9%), Lactobacillus helveticus (3.9%), Lactobacillus camelliae (3.9%), Lactobacillus fermentum (5.8%), Lactobacillus manihotivorans (6.8%), Lactobacillus plantarum (10.7%), Lactobacillus harbinensis (16.5%) and Lactobacillus paracasei subsp. paracasei (35.0%). This study has shown that TP can be well preserved for 90 days by ensilaging and that TPS is not only rich in essential nutrients, but that physiological and biochemical properties of the isolates could provide a platform for future design of lactic acid bacteria (LAB) inoculants aimed at improving the fermentation quality of silage.

Crocetin Induces Cytotoxicity in Colon Cancer Cells Via p53-independent Mechanisms

Li, Cai-Yan,Huang, Wen-Feng,Wang, Qun-Li,Wang, Fan,Cai, E.,Hu, Bing,Du, Jia-Cheng,Wang, Jing,Chen, Rong,Cai, Xiao-Jing,Feng, Jing,Li, Hui-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Objective: Crocin has been proposed as a promising candidate for cancer chemoprevention. The purpose of this investigation was to investigate the chemopreventive action and the possible mechanisms of crocin against human colon cancer cells in vitro. Methods: Cell proliferation was examined using MTT assay and the cell cycle distribution fractions were analyzed using fow cytometric analysis after propidium iodide staining. Apoptosis was detected using theTUNEL Apoptosis Detection Kit with laser scanning confocal microscope. DNA damage was assessed using the alkaline single-cell gel electrophoresis assay, while expression levels of p53, cdk2, cyclinA and P21 were examined by Western blot analysis. Results: Treatment of SW480 cells with crocetin (0.2, 0.4, 0.8 mmol/L) for 48 h signifcantly inhibited their proliferation in a concentration-dependent manner. Crocetin (0.8 mmol/L) signifcantly induced cell cycle arrest through p53-independent mechanisms accompanied by P21 induction. Crocetin (0.8 mmol/L) caused cytotoxicity in the SW480 cells by enhancing apoptosis and decreasing DNA repair capacity in a time-dependent manner. Conclusions: This report provides evidence that crocetin is a potential anticancer agent, which may be used as a chemotherapeutic drug.