Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Li Jing‐Jing,Jin Ming‐Hui,Wang Nian‐Meng,Yu Qi‐Tong,Shang Ze‐Yu,Xue Chao‐Bin 한국곤충학회 2020 Entomological Research Vol.50 No.10

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide‐resistance mechanism in DBM. The lab‐selected (Rh) and field‐collected (Rb) flubendiamide‐resistant lines of P. xylostella with resistance ratios of 1889.92‐fold and 1250.97‐fold, respectively, were used, as well as a lab‐reared flubendiamide‐susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the Rh group and 432 upregulated and 565 downregulated gene messengers in the Rb group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the Rh line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the Rb line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the Rh group and 221 upregulated and 155 downregulated proteins in the Rb group. Further analyses found that three CYP and 11 CYP proteins were over‐expressed in Rh and Rb lines, respectively. Four glutathione S‐transferase (GST) and four UGTs were over‐expressed in Rb line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

Grid-friendly Control Strategy with Dual Primary-Side Series-Connected Winding Transformers

Jing Shang,Xiaohong Nian,Tao Chen,Zhenyu Ma 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.3

High-power three-level voltage-source converters are widely utilized in high-performance AC drive systems. In several ultra-power instances, the harmonics on the grid side should be reduced through multiple rectifications. A combined harmonic elimination method that includes a dual primary-side series-connected winding transformer and selective harmonic elimination pulse-width modulation is proposed to eliminate low-order current harmonics on the primary and secondary sides of transformers. Through an analysis of the harmonic influence caused by dead time and DC magnetic bias, a synthetic compensation control strategy is presented to minimize the grid-side harmonics in the dual primary side series-connected winding transformer application. Both simulation and experimental results demonstrate that the proposed control strategy can significantly reduce the converter input current harmonics and eliminates the DC magnetic bias in the transformer.

Coordination Control of Multiple Electrical Excited Synchronous Motors and Its Application in High-Power Metal-Rolling Systems

Jing Shang,Xiaohong Nian,Yong Liu 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.5

This study focuses on the coordination control problem of multiple electrical excited synchronous motor systems. A robust coordination controller is designed on the basis of cross coupling and an interval matrix. The proposed control strategy can deal with load uncertainty. In addition, the proposed control strategy is applied to a high-power metal-rolling system. Simulation and experiment results demonstrate that the proposed control strategy achieves good dynamic and static performance. It also shows better coordination performance than traditional proportional–integral controllers.

Coordination Control of Multiple Electrical Excited Synchronous Motors and Its Application in High-Power Metal-Rolling Systems

Shang, Jing,Nian, Xiaohong,Liu, Yong The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.5

This study focuses on the coordination control problem of multiple electrical excited synchronous motor systems. A robust coordination controller is designed on the basis of cross coupling and an interval matrix. The proposed control strategy can deal with load uncertainty. In addition, the proposed control strategy is applied to a high-power metal-rolling system. Simulation and experiment results demonstrate that the proposed control strategy achieves good dynamic and static performance. It also shows better coordination performance than traditional proportional-integral controllers.

Grid-friendly Control Strategy with Dual Primary-Side Series-Connected Winding Transformers

Shang, Jing,Nian, Xiaohong,Chen, Tao,Ma, Zhenyu The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.3

High-power three-level voltage-source converters are widely utilized in high-performance AC drive systems. In several ultra-power instances, the harmonics on the grid side should be reduced through multiple rectifications. A combined harmonic elimination method that includes a dual primary-side series-connected winding transformer and selective harmonic elimination pulse-width modulation is proposed to eliminate low-order current harmonics on the primary and secondary sides of transformers. Through an analysis of the harmonic influence caused by dead time and DC magnetic bias, a synthetic compensation control strategy is presented to minimize the grid-side harmonics in the dual primary side series-connected winding transformer application. Both simulation and experimental results demonstrate that the proposed control strategy can significantly reduce the converter input current harmonics and eliminates the DC magnetic bias in the transformer.

Structural and functional analyses of the lipase CinB from <i>Enterobacter asburiae</i>

Shang, Fei,Lan, Jing,Liu, Wei,Chen, Yuanyuan,Wang, Lulu,Zhao, Jing,Chen, Jinli,Gao, Peng,Ha, Nam-Chul,Quan, Chunshan,Nam, Ki Hyun,Xu, Yongbin Elsevier 2019 Biochemical and biophysical research communication Vol.519 No.2

<P><B>Abstract</B></P> <P>Lipases are widely present in various plants, animals and microorganisms, constituting a large category of enzymes. They have the ability to catalyze the cleavage of ester bonds. The lipase CinB from <I>Enterobacter asburiae</I> (<I>E. asburiae</I>) is an acetyl esterase. The primary amino acid sequence suggests that the EaCinB protein belongs to the α/β-hydrolase (ABH) superfamily of the esterase/lipase superfamily. However, its molecular functions have not yet been determined. Here, we report the crystal structure of <I>E. asburiae</I> CinB at a 1.45 Å resolution. EaCinB contains a signal peptide, cap domain and catalytic domain. The active site of EaCinB contains the catalytic triad (Ser180-His307-Asp277) on the catalytic domain. The oxyanion hole is composed of Gly106 and Gly107 within the conserved sequence motif HGGG (amino acid residues 106–109). The substrate is accessible between the α1 and α2 helices or the α1 helix and catalytic domain. Narrow substrate pockets are formed by the α2 helix of the cap domain. Site-directed mutagenesis showed that EaCinB-W208H exhibits a higher catalytic ability than EaCinB-WT by approximately nine times. Our results provide insight into the molecular function of EaCinB.</P> <P><B>Highlights</B></P> <P> <UL> <LI> EaCinB structure contains a signal peptide, cap domain and catalytic domain. </LI> <LI> The active site of EaCinB contains the catalytic triad (Ser180-His307-Asp277). </LI> <LI> The oxyanion hole is composed of Gly106 and Gly107. </LI> <LI> Substrate-binding mode of EaCinB are proposed. </LI> <LI> Site-directed mutagenesis showed that EaCinB-W208H exhibits a higher catalytic ability than EaCinB-WT. </LI> </UL> </P>

Carotid Plaque Stiffness Measured with Supersonic Shear Imaging and Its Correlation with Serum Homocysteine Level in Ischemic Stroke Patients

Jing Shang,Wen Wang,Jun Feng,Guo-gang Luo,Ying Dang,Jian Sun,Yan-qiu Yang,Li-tao Ruan 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.1

Objective: To ascertain the feasibility of using shear wave velocity (SWV) in assessing the stiffness of carotid plaque by supersonic shear imaging (SSI) and explore preliminary clinical value for such evaluation. Materials and Methods: Supersonic shear imaging was performed in 142 patients with ischemic stroke, including 76 males and 66 females with mean age of 66 years (range, 45–80 years). The maximum, minimum, and mean values of SWV were measured for 129 carotid plaques. SWVs were compared between echolucent and echogenic plaques. Correlations between SWVs and serum homocysteine levels were investigated. Based on neurological symptom, the surrogate marker of vulnerable plaque (VP), binary logistic regression was performed and area under curve (AUC) of homocysteine only and homocysteine combing SWVmean was calculated respectively. Results: Echogenic plaques (n = 51) had higher SWVs than echolucent ones (n = 78) (SWVmin 3.91 [3.24−4.17] m/s vs. 1.51 [1.04−1.94] m/s; SWVmean, 4.29 [3.98−4.57] m/s vs. 2.09 [1.69−2.41] m/s; SWVmax, 4.67 [4.33−4.86] m/s vs. 2.62 [2.32−3.31] m/s all p values < 0.01). Pearson correlation analysis showed that stiffness of plaques was negatively correlated with homocysteine level. R values for SWVmin, SWVmean, and SWVmax were -0.205, -0.213, and -0.199, respectively. Binary logistic regression analysis showed that sex (p = 0.008), low-density lipoprotein (p = 0.015), triglycerides (p = 0.011), SWVmean (p = 0.004), and hyper-homocysteinemia (p = 0.010) were significantly associated with symptomatic ischemic stroke. Receiver operating characteristic curves revealed that SWVmean combing serum homocysteine level (AUC = 0.67) presented better diagnostic value than serum homocysteine only (AUC = 0.60) for symptomatic ischemic stroke. Conclusion: Supersonic shear imaging could be used to quantitatively evaluate stiffness of both echolucent and echogenic carotid plaques. More importantly, SWVs of plaques were not only correlated to serum homocysteine level, but also associated with symptomatic ischemic stroke, suggesting that SSI might be useful for understanding more about VP.

韓國 漢字語의 中國語 飜譯에 관한 硏究 : 誤譯의 類型 分析을 中心으로

李尙靜(Li Shang-jing) 중앙어문학회 2011 語文論集 Vol.48 No.-

Republic of Korea and People's Republic of China be long to Chinese character cultural sphere. In the history, both Korea and China have been communicated together for a long time. Therefore, both countries also had cultural exchanges including language exchange. Since the time Chinese characters were introduced to Korea, Chinese characters were official notation in Korea until the end of the Joseon Dynasty period(1392-1910).It gave a lot of influences to Korean society. Therefore, a lot of Sino-Korean words using Chinese characters were created. Currently, more than half of Korean vocabularies are Sino-Korean words. However, when people translate between Korean and Chinese, a lot of mistranslations come out because Sino-Korean words and Chinese words are in the same shape; however Korean and Chinese people's ways of expressions aren't same, which is the major source of creating mistranslations between Korean and Chinese. To describe, Korea and China's same shape words can be divided into 2types; same shape words with same meaning and same shape words with different meaning. The basic reasons of mistranslating between Korean and Chinese can be described as 3 causes; differences of word class, differences of meaning, and differences of expressions. In order to divide into detailed articles, differences of meaning can be divided into similar words and same shape words with different meaning. Also difference of usage of same shape of words, words with same meaning or different meaning, in Korea and China also can be incorporated into detailed articles. For here, I focused on arranging and analyzing summaries of mistranslating between Korean and Chinese. For ways and techniques of translating, I expect further researches.

Synthesis, characterization, and visible light induced photoactivity of tourmaline-N-TiO2 composite for photooxidation of ethylene

Jing-Hua Tzeng,Chih-Huang Weng,Yu-Hao Lin,Shang-Ming Huang,Li-Ting Yen,Jin Anotai,Yao-Tung Lin 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

A novel visible light driven tourmaline-nitrogen-doped-TiO2 composite (S-N-TiO2) was prepared using afacile impregnation and sol gel method and its photocatalytic reaction scheme with ethylene wasproposed. X-ray diffraction analysis confirmed the presence of TiO2 in the form of anatase phase. Scanning electron microscope and energy dispersive spectrometer mapping showed that the TiO2particles were deposited and dispersed on the surface of tourmaline. Under visible light irradiation, the SN-TiO2 catalyst containing 4 wt.% tourmaline has higher photocatalytic activity for the oxidation ofethylene than pure TiO2 and N-doped-TiO2 (N-TiO2). This enhanced activity could be not only attributedto the narrowed band gap in visible light driven N-TiO2, but also improved by the spontaneous electricfield of tourmaline which was applied to restrain the recombination of the electron–hole pairs. Thephotogenerated electrons from N-TiO2 were induced by electricfield to react with ethylene, and theleaving photogenerated holes also formed the reactive species. The photocatalytic activity of S-N-TiO2 ismuch affected by synthesis conditions. This novel S-N-TiO2 photocatalyst has a promising perspective inthe gas treatment for air pollution control and horticultural product industries.

Identification of pepper genes involved in the response to CO 2 enrichment using RNA-Seq analysis

Jing Zhang,Rui Bai,Mengya Shang,Xiaoyong Xu,Hongxia Song,Shaowen Zheng,Leiping Hou,Meilan Li,Guoming Xing 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.1

Pepper is widely cultivated, and the application of CO 2 promotes photosynthesis and increases its yield. However, the molecularmechanisms underlying this are still unclear. In this study, the photosynthetic correlation indexes under elevated CO 2and control conditions were compared. The application of CO 2 increased the photosynthetic capacity of pepper. Moreover,RNA-Seq analysis was used to identify genes that were diff erentially expressed between pepper leaves grown in CO 2 -enrichedconditions and those grown in control conditions. The 149 diff erentially expressed genes (DEGs) were found to be involvedin photosynthesis and other metabolic processes. According to GO signifi cant enrichment analysis, the proteins encodedby the DEGs were mainly found to be located in the chloroplast, the chloroplast matrix, and the apoplast. According toKEGG signifi cant enrichment analysis, the DEGs were found to be involved in glutathione metabolism; starch and sucrosemetabolism; and stilbenoid, diarylheptanoid, gingerol, fl avonoid, and phenylpropanoid biosynthesis. The DEGs were alsoinvolved in the pentose phosphate pathway, carbon metabolism, and porphyrin and chlorophyll metabolism. Based on theGO annotation and the KEGG database analysis, ten of the DEGs identifi ed were suggested to be involved in photosynthesisand related processes; these genes were predicted to have roles in carbohydrate, soluble sugar, and glutathione metabolism,and in raffi nose, cysteine, nucleotide, and ABA biosynthesis. These DEGs are involved in the pentose phosphate pathwayand tricarboxylic acid cycle of carbon assimilation during photosynthesis. One of the DEGs was also found to be involved inchlorophyll biosynthesis. These results lay the foundation for further investigation of the molecular mechanisms and genesinvolved in the response to CO 2 enrichment in peppers.