A detection method of Escherichia coli O157:H7 based on immunomagnetic separation and aptamers-gold nanoparticle probe quenching Rhodamine B’s fluorescence

Fengnan Lian,Dan Wang,Shuo Yao,Lirui Ge,Yue Wang,Yuyi Zhao,Jinbin Zhao,Xiuling Song,Chao Zhao,Jinhua Li,Yajuan Liu,Minghua Jin,Kun Xu 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.8

This research aimed to detect Escherichia coli O157:H7 in milk based on immunomagnetic probe separation technology and quenching effect of gold nanoparticles to Rhodamine B. Streptavidin-modified magnetic beads (MBs) were combined with biotin-modified antibodies to capture E. coli O157:H7 specifically. Gold nanoparticle (AuNPs) was incubated with sulfhydryl-modified aptamers (SH-Aptamers) to obtain the Aptamers-AuNPs probe. After magnetic beads captured target bacteria and formed a sandwich structure with the gold nanoprobe, Rhodamine B was added into complex to obtain fluorescent signal changes. Our results demonstrated that the established method could detect E. coli O157:H7 in the range of 101–107 CFU/mL, and the limit of detection (LOD) was 0.35 CFU/mL in TBST buffer (pH = 7.4). In milk simulation samples, the LOD of this method was 1.03 CFU/mL. Our research provides a promising approach on the detection of E. coli O157:H7.

Stability Analysis and Evaluation of ESR for Hysteresis Controlled Buck Converter

Jinlong WANG,Jinbin ZHAO,Jianfeng DAI,Keqing QU,Tiankai YE 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Buck converter using hysteresis controller has been widely used for power supply in industry applications owing to its instantaneous load transient. However the equivalent series resistance (ESR) of output capacitor has a significant effect for the stability performance. In this paper, the critical value of ESR is analyzed and calculated in the hysteresis controlled buck converter. Finally, simulation results indicate that the larger output capacitor ESR is necessary to make sure the converter operate normally, otherwise, the converter will operate abnormally.

A Decupled PQ Control strategy of Voltage - Controlled Inverters

Yang Chen,Jinbin Zhao,Jinlong Wang,Keqing Qu,Shuuichi Ushiki,Masaaki Ohshima 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

This paper deals with problems caused by high distributed generation penetration and proposes a simple and precise PQ control strategy for voltage-controlled inverters in micro grids. The widely-used current-controlled inverters cannot provide voltage support for micro grids and have to develop seamless transfer strategy between gird connected mode and islanded mode. A voltage-controlled inverter is raised in the paper due to the problems. The new inverter can be regarded as a voltage source cascaded with controllable output impedance. By adopting a new phase-locked loop, the output voltage amplitude and frequency are well decoupled. Thus output active and reactive powers are decoupled satisfactory. The output response is fast and precise. The idea is verified by simulation and experiment results.

PQ Control of Micro Grid Inverters with Axial Voltage Regulators

Chen, Yang,Zhao, Jinbin,Qu, Keqing,Li, Fen The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.6

This paper presents a PQ control strategy for micro grid inverters with axial voltage regulators. The inverter works in the voltage-controlled mode and can help improve the terminal power quality. The inverter has two axial voltage regulators. The 1st regulator involves the output voltage amplitude and output impedance, while the 2nd regulator controls the output frequency. The inverter system is equivalent to a controllable voltage source with a controllable inner output impedance. The basic PQ control for micro grid inverters is easy to accomplish. The output active and reactive powers can be decoupled well by controlling the two axial voltages. The 1st axial voltage regulator controls the reactive power, while the 2nd regulator controls the active power. The paper analyses the axial voltage regulation mechanism, and evaluates the PQ decoupling effect mathematically. The effectiveness of the proposed control strategy is validated by simulation and experimental results.

Grid-friendly Characteristics Analysis and Implementation of a Single-phase Voltage-controlled Inverter

Zhang, Shuaitao,Zhao, Jinbin,Chen, Yang,He, Chaojie The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.5

Inverters are widely used in distributed power generation and other applications. However, their lack of inertia and variable impedance may cause system instability and power transfer inaccuracy. This paper proposes a control scheme for a single phase voltage-controlled inverter with some grid-friendly characteristics. The proposed control algorithm enables the inverter to function as a voltage source with an inner output impedance in both the islanded and grid-connected modes. Virtual inertia and rotor equations are embedded in the PLL part. Thus, the frequency stability can remain. The inner output impedance can be adjusted freely, which helps to accurately decouple and transmit the output active and reactive power. The proposed inverter operates like a traditional synchronous generator. Simulations and experiments are designed and carried out to verify the proposed control strategy.

Grid-friendly Characteristics Analysis and Implementation of a Single-phase Voltage-controlled Inverter

Shuaitao Zhang,Jinbin Zhao,Yang Chen,Chaojie He 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.5

Inverters are widely used in distributed power generation and other applications. However, their lack of inertia and variable impedance may cause system instability and power transfer inaccuracy. This paper proposes a control scheme for a single phase voltage-controlled inverter with some grid-friendly characteristics. The proposed control algorithm enables the inverter to function as a voltage source with an inner output impedance in both the islanded and grid-connected modes. Virtual inertia and rotor equations are embedded in the PLL part. Thus, the frequency stability can remain. The inner output impedance can be adjusted freely, which helps to accurately decouple and transmit the output active and reactive power. The proposed inverter operates like a traditional synchronous generator. Simulations and experiments are designed and carried out to verify the proposed control strategy.

Prediction of Pathologic Response to Neoadjuvant Chemoradiotherapy in Patients with Esophageal Squamous Cell Carcinoma Incorporating Hematological Biomarkers

Yingjia Wu,Jinbin Chen,Lei Zhao,Qiaoqiao Li,Jinhan Zhu,Hong Yang,Suping Guo,Mian Xi 대한암학회 2021 Cancer Research and Treatment Vol.53 No.1

Purpose This study aimed to develop a nomogram for predicting pathologic complete response (pCR) after neoadjuvant chemoradiotherapy (CRT) in patients with esophageal squamous cell carcinoma (ESCC) by integrating hematological biomarkers and clinicopathological characteristics. Materials and Methods Between 2003 and 2017, 306 ESCC patients who underwent neoadjuvant CRT followed by esophagectomy were analyzed. Besides clinicopathological factors, hematological parameters before, during, and after CRT were collected. Univariate and multivariate logistic regression analyses were performed to identify predictive factors for pCR. A nomogram model was built and internally validated. Results Absolute lymphocyte count (ALC), lymphocyte to monocyte ratio, albumin, hemoglobin, white blood cell, neutrophil, and platelet count generally declined, whereas neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) increased significantly following neoadjuvant CRT. After surgery, 124 patients (40.5%) achieved a pCR. The pCR group demonstrated significantly more favorable survival than the non-pCR group. On multivariate analysis, significant factors associated with pCR included sex, chemotherapy regimen, post-CRT endoscopic finding, pre-CRT NLR, ALC nadir during CRT, and post-CRT PLR, which were incorporated into the prediction model. The nomogram indicated good accuracy in predicting pCR, with a C-index of 0.75 (95% confidence interval, 0.71 to 0.78). Conclusion Female, chemotherapy regimen of cisplatin/vinorelbine, negative post-CRT endoscopic finding, pre-CRT NLR (≤ 2.1), ALC nadir during CRT (> 0.35×109/L), and post-CRT PLR (≤ 83.0) were significantly associated with pCR in ESCC patients treated with neoadjuvant CRT. A nomogram incorporating hematological biomarkers to predict pCR was developed and internally validated, showing good predictive performance.

Power Control Strategies for Single-Phase Voltage-Controlled Inverters with an Enhanced PLL

Gao, Jiayuan,Zhao, Jinbin,He, Chaojie,Zhang, Shuaitao,Li, Fen The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.1

For maintaining a reliable and secure power system, this paper describes the design and implement of a single-phase grid-connected inverter with an enhanced phase-locked loop (PLL) and excellent power control performance. For designing the enhanced PLL and power regulator, a full-bridge voltage-controlled inverter (VCI) is investigated. When the grid frequency deviates from its reference values, the output frequency of the VCI is unstable with an oscillation of 2 doubling harmonics. The reason for this oscillation is analyzed mathematically. This oscillation leads to an injection of harmonics into the grid and even causes an output active power oscillation of the VCI. For eliminating the oscillation caused by a PLL, an oscillation compensation method is proposed. With the proposed method, the VCI maintains the original PLL control characteristics and improves the PLL robustness under grid frequency deviations. On the basis of the above analysis, a power regulator with the primary frequency and voltage modulation characteristics is analyzed and designed. Meanwhile, a small-signal model of the power loops is established to determine the control parameters. The VCI can accurately output target power and has primary frequency and voltage modulation characteristics that can provide active and reactive power compensation to the grid. Finally, simulation and experimental results are given to verify the idea.

PQ Control of Micro Grid Inverters with Axial Voltage Regulators

Yang Chen,Jinbin Zhao,Keqing Qu,Fen Li 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.6

This paper presents a PQ control strategy for micro grid inverters with axial voltage regulators. The inverter works in the voltage-controlled mode and can help improve the terminal power quality. The inverter has two axial voltage regulators. The 1st regulator involves the output voltage amplitude and output impedance, while the 2nd regulator controls the output frequency. The inverter system is equivalent to a controllable voltage source with a controllable inner output impedance. The basic PQ control for micro grid inverters is easy to accomplish. The output active and reactive powers can be decoupled well by controlling the two axial voltages. The 1st axial voltage regulator controls the reactive power, while the 2nd regulator controls the active power. The paper analyses the axial voltage regulation mechanism, and evaluates the PQ decoupling effect mathematically. The effectiveness of the proposed control strategy is validated by simulation and experimental results.

Evaluating optimum tilt angle for PV modules using solar radiation models in Wuhan, China

Fen. Li,Nianjun. Ma,Jinbin. Zhao,Keqing. Qu,Xingwu. Yang,Zhenghong. Chen 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Four models are used to estimate the hourly solar radiation on 15 pieces of different tilted photovoltaic (PV) modules in Wuhan, China based on power and solar radiation data during a whole year. The optimum tilt angle (OTA) in Wuhan for yearly and semi-yearly adjustment was determined. Then it was determined that the best mode is the tilt angle of PV module adjusted every half a year. In semi-yearly adjustment, OTA in semi-year of winter and summer is found as 45°, 15°, respectively. Experimental results verify the theoretical calculated values of the OTA with high precision. Energy radio (ER) reveals PV modules with adjustment of tilt angle twice a year has a capability to improve nearly 7% in comparison with fixed type. The best model was chosen based on test results from statistical indicators. The statistical results demonstrate that among the studied models, Liu&Jordan model is the most accurate.