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다이젠펑 ( Dai Jianfeng ) 중국사학회 2021 中國史硏究 Vol.- No.133
1980년대부터 현재에 이르기까지 沈家本의 사상에 대한 학문적 연구는 일반적으로 법률 사상에서 제도적 문화, 법률 교육 및 법률 연구 방법에 이르기까지 다차원적인 진전을 보여 왔다. 헌법사상의 개괄에 대해서는 아직 논의의 여지가 많다. 沈家本은 중국과 서양의 법 이론과 법치의 실천을 이해하였으며, 이는 모두 그의 헌법 사상에 녹아있다. 沈家本의 헌법 사상은 각각 분권화를 수단으로, 법치와 사법 독립을 핵심으로, 민족 부흥과 민권 보호를 목적으로 하는 종합적인 사상 체계이다. 구법을 개정하는 한편 신법을 제정하고 사법부를 개혁하는 등 제도를 정비하고 인재를 양성하여 중국법 근대화의 서곡을 여는 기회로 삼았다. 헌법사상과 그 실현은 현대의 법치 개혁을 위한 중요한 준거로서의 가치를 지니고 있다. 사회 변혁을 위한 법적 기반을 마련하고, 제도 개혁과 인재 양성을 강화하는 한편, 국가 권력 배분에 대한 종합적인 연구를 추진하여 국가 부흥과 인민의 권리 보호의 취지를 견지함으로써 꾸준히 사회 발전을 이룩하여야 한다. From the 1980s to the present, the academic research on Shen Jiaben's thought has generally presented a scene of multi-dimensional advancement from legal thoughts to institutional culture, as well as legal education and legal research methods. As for the general outline of constitutional thought, there is still much room for discussion. Behind Shen Jiaben’s synthesis of Chinese and Western legal theories and legal practices, all are permeated with constitutional thoughts. Shen Jiaben's constitutional thought is a comprehensive ideological system composed of decentralization as a means, rule of law and judicial independence as the core, and national rejuvenation and protection of civil rights as the purpose. It took the opportunity of preparatory institutions and talent cultivation, from revising old laws, establishing new laws to reforming the judiciary, thus unveiling the prelude to the modernization of Chinese law. The whole constitutional thought and its realization path have significant reference value for the modern rule of law reform. To a certain extent, it enlightens us: it is necessary to establish a more comprehensive rule of law system based on the needs of the public in society, and to lay a legal foundation for social transformation; and there is a need to strengthen institutional reforms and talent cultivation, and promote comprehensive research on the allocation of state power; moreover, adhere to the main thrust of national rejuvenation and the protection of people's rights to achieve social development steadily.
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Tissue-Specific CRISPR/Cas9 System of Cotton Pollen with GhPLIMP2b and GhMYB24 Promoters
Lei Jianfeng,Dai Peihong,Li Jiyang,Yang Mi,Li Xiuqin,Zhang Wanqi,Zhou Guantong,WangzhenGuo,Liu Xiaodong 한국식물학회 2021 Journal of Plant Biology Vol.64 No.1
CRISPR/Cas9 technology is a powerful tool for improving crop genetic traits. However, CRISPR/Cas9 system for efficient expression of cotton germ cells has still not been established. In this study, we developed a tissue-specific vectors to drive Cas9 expression with GhPLIMP2b and GhMYB24 promoters and established the effective method to transform cotton pollen by Agrobacterium vacuum infiltration. GhPLIMP2b and GhMYB24 promoters of cotton pollen were cloned into Cas9 expression vectors. The sgRNAs targetting to CLA1, ERA1 and GGB (drought-resistant negative regulation genes) were designed and constructed into GhPLIMP2b and GhMYB24 promoter vectors. Cotton pollens were tranformed by Agrobacterium vacuum infiltration with GhPLIMP2b and GhMYB24 promoter vectors. The results of clone sequencing shown that mutation types of the sequence were mainly base substitutions wth the frequency from 3.29 to 6.45%. Eleven potential off-target sites were chosen and two sites were observed. Our results indicated that GhPLIM2bP::Cas9 and GhMYB24P::Cas9 editing vectors achieved targeted edition of endogenous genes in cotton pollen, but there were a few off-target effects. This study provides an effective gene-editing system for the rapid acquisition of cotton mutants using pollen as a transgenic receptor.
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Stability Analysis and Evaluation of ESR for Hysteresis Controlled Buck Converter
Jinlong WANG,Jinbin ZHAO,Jianfeng DAI,Keqing QU,Tiankai YE 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6
Buck converter using hysteresis controller has been widely used for power supply in industry applications owing to its instantaneous load transient. However the equivalent series resistance (ESR) of output capacitor has a significant effect for the stability performance. In this paper, the critical value of ESR is analyzed and calculated in the hysteresis controlled buck converter. Finally, simulation results indicate that the larger output capacitor ESR is necessary to make sure the converter operate normally, otherwise, the converter will operate abnormally.
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