Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

Jin, H.J.,Kim, I.C.,Wee, M.S.,Yeon, S.H.,Kim, C.D.,Lee, S.S.,Cho, C.Y.,Cho, S.R.,Son, D.S.,Park, C.K. 韓國受精卵移植學會 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

H. J. Jin,I. C. Kim,M. S. Wee,S. H. Yeon,C. D. Kim,S. S. Lee,C. Y. Cho,S. R. Cho,D. S. Son,C. K. Park,Z. D. Li 사단법인 한국동물생명공학회 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire(p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

Production of (S)-3-hydroxybutyrate by metabolically engineered Saccharomyces cerevisiae

Yun, E.J.,Kwak, S.,Kim, S.R.,Park, Y.C.,Jin, Y.S.,Kim, K.H. Elsevier Science Publishers 2015 Journal of biotechnology Vol.209 No.-

(S)-3-Hydroxybutyrate (S-3HB) can be used as a precursor for the synthesis of biodegradable polymers such as polyhydroxyalkanoate and stereo-specific fine chemicals such as antibiotics, pheromones, and drugs. For the production of S-3HB in yeast, the biosynthetic pathway of S-3HB from acetyl-CoA, consisting of the three enzymes, acetyl-CoA C-acetyltransferase (ACCT), acetoacetyl-CoA reductase (ACR), and 3-hydroxybutyryl-CoA thioesterase (HBT), was introduced into Saccharomyces cerevisiae. An engineered yeast strain overexpressing ERG10, hbd, and tesB genes not only exhibited enzyme activities of AACT, ACR, and HBT, but also produced S-3HB from ethanol. In order to increase the titer of S-3HB, a fed-batch fermentation based on pulse feeding of ethanol as a carbon source was performed, and a final S-3HB titer of 12.0g/L was achieved. This is the first report on the production of 3HB by engineered yeast, utilizing ethanol as the carbon source, suggesting that the industrially preferred S. cerevisiae can be a promising host for producing S-3HB.

Characteristics of a Bi-2212 Monofilar Superconducting Fault Current Limiting Element

Jin, H.B.,Li, Z.Y.,Ma, Y.H.,Ryu, K.,Park, K.B.,Oh, I.S. IEEE 2009 IEEE transactions on applied superconductivity Vol.19 No.3

<P>The Bi-2212 monofilar bulk element was selected as a candidate in developing a 24 kV/630 A class superconducting fault current limiting (SFCL) system. This study investigated the Bi-2212 monofilar element both experimentally and theoretically regarding the DC voltage-current characteristics, AC loss characteristics, inductance, current limiting characteristics and local temperature distribution. It was found that the critical current of the Bi-2212 monofilar element increased 3.3 times as the operation temperature decreased from 77 K to 65 K. Although the arrangement of the Bi-2212 conductor was a coil shape, the measured AC loss was at a level similar to what is calculated from the Norris equation at both the temperatures 77 K and 65 K. The temperatures measured at each section during a fault were in good agreement with the results of the numerical analysis. Moreover, the results showed that the monofilar element was not yet sufficiently homogeneous to be used at its full rated voltage.</P>

Melatonin enhances arsenic trioxide-induced cell death via sustained upregulation of Redd1 expression in breast cancer cells

Yun, S.M.,Woo, S.H.,Oh, S.T.,Hong, S.E.,Choe, T.B.,Ye, S.K.,Kim, E.K.,Seong, M.K.,Kim, H.A.,Noh, W.C.,Lee, J.K.,Jin, H.O.,Lee, Y.H.,Park, I.C. North-Holland 2016 Molecular and cellular endocrinology Vol.422 No.-

Melatonin is implicated in various physiological functions, including anticancer activity. However, the mechanism(s) of its anticancer activity is not well understood. In the present study, we investigated the combined effects of melatonin and arsenic trioxide (ATO) on cell death in human breast cancer cells. Melatonin enhanced the ATO-induced apoptotic cell death via changes in the protein levels of Survivin, Bcl-2, and Bax, thus affecting cytochrome c release from the mitochondria to the cytosol. Interestingly, we found that the cell death induced by co-treatment with melatonin and ATO was mediated by sustained upregulation of Redd1, which was associated with increased production of reactive oxygen species (ROS). Combined treatment with melatonin and ATO induced the phosphorylation of JNK and p38 MAP kinase downstream from Redd1 expression. Rapamycin and S6K1 siRNA enhanced, while activation of mTORC1 by transfection with TSC2 siRNA suppressed the cell death induced by melatonin and ATO treatment. Taken together, our findings suggest that melatonin enhances ATO-induced apoptotic cell death via sustained upregulation of Redd1 expression and inhibition of mTORC1 upstream of the activation of the p38/JNK pathways in human breast cancer cells.

Enhanced ethanol fermentation by engineered Saccharomyces cerevisiae strains with high spermidine contents

Kim, S. K.,Jo, J. H.,Jin, Y. S.,Seo, J. H. Springer Science + Business Media 2017 Bioprocess and biosystems engineering Vol.40 No.5

<P>Construction of robust and efficient yeast strains is a prerequisite for commercializing a biofuel production process. We have demonstrated that high intracellular spermidine (SPD) contents in Saccharomyces cerevisiae can lead to improved tolerance against various fermentation inhibitors, including furan derivatives and acetic acid. In this study, we examined the potential applicability of the S. cerevisiae strains with high SPD contents under two cases of ethanol fermentation: glucose fermentation in repeated-batch fermentations and xylose fermentation in the presence of fermentation inhibitors. During the sixteen times of repeated-batch fermentations using glucose as a sole carbon source, the S. cerevisiae strains with high SPD contents maintained higher cell viability and ethanol productivities than a control strain with lower SPD contents. Specifically, at the sixteenth fermentation, the ethanol productivity of a S. cerevisiae strain with twofold higher SPD content was 31% higher than that of the control strain. When the SPD content was elevated in an engineered S. cerevisiae capable of fermenting xylose, the resulting S. cerevisiae strain exhibited much 40-50% higher ethanol productivities than the control strain during the fermentations of synthetic hydrolysate containing high concentrations of fermentation inhibitors. These results suggest that the strain engineering strategy to increase SPD content is broadly applicable for engineering yeast strains for robust and efficient production of ethanol.</P>

라온 극저온제어시스템 기반설비와 제어화면 구축 현황

윤성운(S. YOON),이상일(S. LEE),박미정(M. J. PARK),손창욱(C. W. SOHN),최용준(Y. CHOI),유정현(J. YOO),Lingxue JIN,김무상(M. KIM),김도균(D. KIM),기태경(T. KI),서미경(M. K. SEO),진민성(M. S. JIN) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11

RAON, a heavy ion superconducting linear accelerator has been installed in Daejeon, S. Korea. A cryogenic system, which was installed and tested its performance cool the accelerator down until 269 K. The system consists of a lot of components, cryogenic plants, a distribution box, cryogenic distribution lines, cryomodules and valve boxes. A cryogenic control system which is complicated and integrated by EPICS (Experimental Physics and Industrial Control System) is developed and the graphic user interface which is designed by CS-studio (Control System-studio) is developed. They were verified during the accelerator cool-down but they need some modification based on the cool-down operation. This paper summarizes the RAON cryogenic system infrastructure and graphic user interfaces.

Biochemical, pharmaceutical and therapeutic properties of long-acting lithocholic acid derivatized exendin-4 analogs

Chae, S.Y.,Jin, C.H.,Shin, J.H.,Son, S.,Kim, T.H.,Lee, S.,Youn, Y.S.,Byun, Y.,Lee, M.S.,Lee, K.C. Elsevier Science Publishers 2010 Journal of controlled release Vol.142 No.2

Alterations in the physicochemical characteristics of peptide drugs can transform their biological and pharmaceutical features. In the present study, we explored the potentials of lithocholic acid (LCA)-modified exendin-4 derivatives as novel long-acting GLP-1 receptor agonists. Exendin-4 was modified with lithocholic acid at two lysine residues to produce three derivatives that were obtained by reverse-phase HPLC separation, namely, Lys<SUP>12</SUP>-LCA-exendin-4 (LCA-M2), Lys<SUP>27</SUP>-LCA-exendin-4 (LCA-M1), and Lys<SUP>12,27</SUP>-LCA-exendin-4 (LCA-Di)). The biological, pharmacological, and physicochemical characteristics of these three exendin-4 analogues were then investigated. Although slight reductions in the GLP-1 receptor binding capacity and insulinotropic activity of exendin-4 were observed after derivatization, the mono-LCA substitutions, especially LCA-M1, well-preserved antidiabetic activity in type 2 diabetic mice when administered subcutaneously or intraperitoneally. Furthermore, the pharmacokinetic characteristics were dramatically enhanced, that is, absorption was delayed and elimination half-life was increased (1.6+/-0.4 and 9.7+/-1.4h by exendin-4 and LCA-M1, respectively). The enhanced long-acting characteristics of the derivative was found to be due to albumin binding and nanoparticle formation, and these were verified by the restoration of normoglycemia in type 2 diabetic mice after single injection (>24h, >10nmol/kg, s.c.) and daily injections (15nmol/kg/day) maintained normoglycemia for the 4-week administration period. Furthermore, antidiabetic potentials, such as, glucose clearance kinetics and percentage areas occupied by pancreatic β-cells were also enhanced by long-term LCA-M1 administration. The present study demonstrates that the derivatization of exendin-4 with LCA offers a possible means of producing a long-acting GLP-1 receptor agonist.

ANALYSIS OF HIGH - FIELD MAGNETIZATION PROCESS IN Sm₂Fe17N3.0

T. S. Zhao,H. M. Jin,J. I. Lee,K. S. Pang 한국자기학회 1995 韓國磁氣學會誌 Vol.5 No.5

The observed high-field magnetization curves of Sm₂Fe_(17)N_(3.0) at 4.2 K and 296 K are well reproduced by the calculation using the Sm-Fe exchange field 2_(μB)Hex = 320 K and two crystalline electric field parameters A²_0 = -910 K and A^0_1 = 200 K. The calculation shows that during the magnetization process along the hard axis at 4.2 K, the Sm moment rotates toward the direction anti parallel to H when H < 110 kOe and then returns to the field direction with further increase of the field. At 296 K, the Sm moment rotates toward the direction antiparallel to H monotonously with increasing field and finally becomes antiparallel to H when H ≥ H_A = 210 kOe. The particular magnetization process of the Sm moment can be explained by the field-induced noncollinear coupling between the spin and orbital moments of the Sm ion.

Nematicidal effect of Sparassis latifolia-derived sparassol and disodium sparassol against Bursaphelenchus xylophilus

Lee, S.K.,Lee, K.T.,Park, Y.B.,Jin, G.H.,Ka, K.H.,Seo, S.T. 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol. No.

<P>The pine wood nematode (Bursaphelenchus xylophilus) causes pine wilt disease, which is a major problem affecting pine forests. This study aimed to identify metabolites from Sparassis latifolia that have nematicidal activity and to investigate the effect of water-soluble formulations of the active compounds for use as trunk-injectable control agents against the nematode B. xylophilus. When the submerged cultures of S. latifolia were bioassayed against B. xylophilus, strain KFRI 747 showed the most potent activity (52.8%), followed by KFRI 1080 (44.4%) and KFRI 645 (34.8%); their activities were correlated with the concentration of sparassol in their mycelia. Six fractions (SLE1-6) were obtained from ethyl acetate extracts of the liquid culture medium; nematicidal activity was found only in SLE4 (which included sparassol). Sparassol bioassayed against B. xylophilus and had LC50 and LC95 of 84.92 and 132.13 ppm, respectively. Sparassol was modified to disodium sparassol using NaOH because sparassol was not water-soluble and thus was not useful as an agent for trunk injection. Disodium sparassol exhibited similar nematicidal activity to that found with sparassol. The trunk-injectable nematicides (abamectin and emamectin benzoate) were more effective than sparassol and disodium sparassol in terms of LC50 and LC95 values. However, sparassol has a more simple chemical structure than these compounds and could be more easily synthesized. To our knowledge, this is the first report indicating that disodium sparassol might be useful as nematicide for use against the pine wood nematode. (C) 2015 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.</P>