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      • 장려품종 콩을 이용한 메주 및 된장 품질 특성

        이가순,이주찬,이종국,황의선,오만진,이승수 한국식품저장유통학회(구 한국농산물저장유통학회) 2002 한국식품저장유통학회지 Vol.9 No.2

        국산 콩 재배를 장려하고 메주 및 된장가공용으로 적합한 우수 콩품종을 선발하고자 충남도내에서 주로 재배되고 있는 대원, 소담, 진품 및 황금 콩을 이용하여 메주 및 된장을 제조한 후 품종간 품질의 특성을 조사하였다. 원료 콩들의 일반성분 중 조지방은 16.89∼19.31%, 조단백질은 26.7∼30.5%,이었고 품종별 총 유리당함량은 4.99∼5.26%로 주된 유리당은 stachyose으로서 2.59∼3.51%를 함유하고 있었고 그 다음은 raffinose와 sucrose순이었다. 메주의 조지방은 16.2∼21.0%로 황금콩이, 조단백질은 32.2∼36.4%로 대원과 소담콩이, 가용성무질소물은 23.5∼26.62%로 진품콩이 각각 함량이 높았으며 아미노태 질소함량은 266∼ 371 ㎎%로 대원콩이 가장 높았다. 메주의 총 유리당은 2.10∼2.66%로 주된 유리당은 glucose이었으며 대원콩이 유리당함량이 높았다. 메주덩어리의 색도를 본 결과 황금콩이 밝기가 가장 감소하였으며 적색도는 대원콩과 황금콩이 높았고 황색도는 소담콩이 가장 높게 나타났다. 또 메주를 분쇄하여 메주분으로 조사한 결과 소담콩이 밝기값이 가장 높았고 적색도가 가장 낮았으며 황색도는 황금콩이 가장 높았다. 발효된장의 조지방은 7.90∼9.71%로 진품콩이, 조단백은 9.67∼13.50%로 대원콩이, formol태 질소함량은 2739∼3825㎎%로 소담콩이, 조사포닌 함량은 6.88∼8.36%로 대원콩이 각각 함량이 가장 높았으며 총유리당 함량은 1.88∼2.22%으로 황금콩이 가장 높았으며 된장의 주된 유리당은 fructose와 glucose이었다. 된장제품의 색도 중 밝기와 황색도는 소담콩이 가장 높았으며 적색도는 황금콩이 가장 높았다. 된장제품의 관능검사결과 소담콩이 색깔면에서는 우수하였으나 맛, 질감 및 전반적인 기호도를 볼 때 황금콩이 가장 좋은 것으로 평가되었다. In order to selection of the suitable soybean varieties, the quality characteristics of meju and doenjang on 4 domestic soybean cultivars(Daeweon, Sodam, Jinpeum and Hwangkeum) were evaluated. Crude protein and lipid content was 26.7∼30.5% and 16.89∼19.31%. The content of total free sugar among 4 soybean cultivars were 4.99∼5.26% and composed mainly stachyose(2.59∼3.51%), raffinose and sucrose. The content of total free sugar of meju were the highest in Jinpeum among 2.10∼2.66% and composed mainly glucose. The lightness of meju lump in Hwangkeum was larger decrease than the others, the redness were higher in Daeweon and Hwangkeum, the yellowness was the highest in Sodam. The lightness of meju powder was the highest in Sodam, the redness was the lowest in Sodam, the yellowness was the highest in Hwangkeum. The chemical composition of doenjang were that : was the highest in Jinpeum among the 7.90∼9.71% crude lipid, in Daeweon among 9.67∼13.50% crude protein, in Sodam among 2739∼3825 ㎎% Fromol nitrogen and in Daeweon among 6.88∼8.36% crude saponin, respectively. The content of total free sugar of doenjang were the highest in Hwangkeum among 1.88∼2.22% and composed mainly fructose and glucose. The lightness and yellowness of doenjang was the highest in Sodam, the redness was the highest in Hwangkeum. Doenjang by Hwangkeum had good score for taste, texture and overall quality in sensory evaluation.

      • Computer-Aided Detection of Lung Nodules: Influence of the Image Reconstruction Kernel for Computer-Aided Detection Performance

        Hwang, Jiyoung,Chung, Myung Jin,Bae, Younga,Shin, Kyung Min,Jeong, Sun Young,Lee, Kyung Soo Lippincott Williams Wilkins, Inc. 2010 Journal of computer assisted tomography Vol.34 No.1

        OBJECTIVE:: To evaluate the relationship between a computed tomographic reconstruction kernel and the sensitivity of a computer-aided detection (CAD) system for lung nodule detection. METHODS:: We retrospectively studied 36 consecutive patients with no known pulmonary nodules who underwent low-dose computed tomography for lung cancer screening with 3 different reconstruction kernels (B, C, and L). All series were reviewed with a commercial CAD system for lung nodule detection. RESULTS:: The 36 scans showed 231 uncalcified nodules (170 micronodules and 61 nodules). There was little variation of sensitivities for each series (82%, 88%, and 82% for the nodules of B, C, and L, respectively). When the results of 2 series were combined, sensitivities were boosted (B + C, 89%; B + L, 95%; and C + L, 96% for the nodules). CONCLUSIONS:: Sensitivity of the CAD system was influenced by the selection of the reconstruction kernel. By combining data from 2 different kernels, CAD sensitivity can be elevated without further patient radiation exposure.

      • KCI등재
      • Dry etching of polydimethylsiloxane using microwave plasma

        Hwang, Sung Jin,Oh, Dong Joon,Jung, Phill Gu,Lee, Sang Min,Go, Jeung Sang,Kim, Joon-Ho,Hwang, Kyu-Youn,Ko, Jong Soo IOP 2009 JOURNAL OF MICROMECHANICS AND MICROENGINEERING - Vol.19 No.9

        <P>This paper presents a new polydimethylsiloxane (PDMS) dry-etching method that uses microwave plasma. The applicability of the method for fabricating microstructures and removing residual PDMS is also verified. The etch rate of PDMS was dominantly influenced by the gas flux ratio of CF<SUB>4</SUB>/O<SUB>2</SUB> and the microwave power. While the PDMS etch rate increased as the flux ratio of CF<SUB>4</SUB> was increased, the etch rate decreased as the flux ratio of O<SUB>2</SUB> was increased. The maximum etch rate of 4.31 µm min<SUP>−1</SUP> was achieved when mixing oxygen (O<SUB>2</SUB>) and tetrafluoromethane (CF<SUB>4</SUB>) at a 1:2 ratio at 800 W power. The PDMS etch rate almost linearly increased with the microwave power. The ratio of the vertical etch rate to the lateral etch rate was in a range of 1.14–1.64 and varied with the gas fluxes. In consideration of potential applications of the proposed PDMS etching method, array-type PDMS microwells and network-type microprotrusion structures were fabricated. The contact angle was dramatically increased from 104° (non-etched PDMS surface) to 148° (etched PDMS surface) and the surface was thereby modified to be superhydrophobic. In addition, a thin PDMS skin that blocked holes and PDMS residues affixed in nickel microstructures was successively removed.</P>

      • KCI등재

        Microbacterium elymi sp. nov., Isolated from the Rhizospheric Soil of Elymus tsukushiensis, a Plant Native to the Dokdo Islands, Republic of Korea

        Hwang Ye-Ji,Lee Soo-Yeong,Son Jin-Soo,Youn Jin-suk,Lee Woong,Shin Jae-Ho,Lee Mi-Hwa,Ghim Sa-Youl 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.2

        Microbacterium elymi KUDC0405T was isolated from the rhizosphere of Elymus tsukushiensis from the Dokdo Islands. The KUDC0405T strain was Gram-stain-positive, non-spore forming, non-motile, and facultatively anaerobic bacteria. Strain KUDC0405T was a rod-shaped bacterium with size dimensions of 0.3–0.4 × 0.7–0.8 μm. Based on 16S rRNA gene sequences, KUDC0405T was most closely related to Microbacterium bovistercoris NEAU-LLET (97.8%) and Microbacterium pseudoresistens CC-5209T (97.6%). The dDDH (digital DNA-DNA hybridization) values between KUDC0405T and M. bovistercoris NEAU-LLET and M. pseudoresistens CC-5209T were below 17.3% and 17.5%, respectively. The ANI (average nucleotide identity) values among strains KUDC0405T , M. bovistercoris NEAU-LLET , and M. pseudoresistens CC-5209T were 86.6% and 80.7%, respectively. The AAI (average amino acid identity) values were 64.66% and 64.97%, respectively, between KUDC0405T and its closest related type strains. The genome contained 3,596 CDCs, three rRNAs, 46 tRNAs, and three non-coding RNAs (ncRNAs). The genomic DNA GC content was 70.4%. The polar lipids included diphosphatydilglycerol, glycolipid, phosphatydilglycerol, and unknown phospholipid, and the major fatty acids were anteiso-C17:0 and iso-C16:0. Strain KUDC0405T contained MK-12 as the major menaquinone. Based on genotypic, phylogenetic, and phenotypic properties, strain KUDC0405T should be considered a novel species within the genus Microbacterium, for which we propose the name M. elymi sp. nov., and the type strain as KUDC0405T (=KCTC 49411T , =CGMCC1.18472T ).

      • KCI등재

        Evolutionary Design of Morphology-Based Homomorphic Filter for Feature Enhancement of Medical Images

        Hwang, Hee-Soo,Oh, Jin-Sung Korean Institute of Intelligent Systems 2009 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.9 No.3

        In this paper, a new morphology-based homomorphic filtering technique is presented to enhance features in medical images. The homomorphic filtering is performed based on the morphological sub-bands, in which an image is morphologically decomposed. An evolutionary design is carried to find an optimal gain and structuring element of each sub-band. As a search algorithm, Differential Evolution scheme is utilized. Simulations show that the proposed filter improves the contrast of the interest feature in medical images.

      • SCIESCOPUSKCI등재

        WEAK SOLUTIONS OF THE EQUATION OF MOTION OF MEMBRANE WITH STRONG VISCOSITY

        Hwang, Jin-Soo,Nakagiri, Shin-Ichi Korean Mathematical Society 2007 대한수학회지 Vol.44 No.2

        We study the equation of a membrane with strong viscosity. Based on the variational formulation corresponding to the suitable function space setting, we have proved the fundamental results on existence, uniqueness and continuous dependence on data of weak solutions.

      • SCIESCOPUSKCI등재

        Panax ginseng Improves Senile Testicular Function in Rats

        Hwang, Seock-Yeon,Sohn, Sang-Hyun,Wee, Jae-Joon,Yang, Jin-Bae,Kyung, Jong-Soo,Kwak, Yi-Seong,Kim, Sung-Won,Kim, Si-Kwan The Korean Society of Ginseng 2010 Journal of Ginseng Research Vol.34 No.4

        We reported previously that the administration of Korean red ginseng water extract (KRG-WE) protected the guinea pig testis against damage induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (a potent endocrine disruptor). We also found that crude saponin from ginseng was the active ingredient responsible for this protection. Here, we examined the biological role of KRG-WE in an animal model of age-induced dysfunction of spermatogenesis. Twenty-four male Sprague-Dawley (six 2-month-old and eighteen 12-month-old) rats were used. The young and old control groups received only vehicle. The ginseng saponin (GS)- and KRG-WE-treated groups received GS (40 mg/kg body weight/day) and KRG-WE (200 mg/kg body weight/day), respectively, for 4 months. The number of cells, Sertoli cell index, Johnsen's score, and sex hormone levels decreased significantly with age. However, the administration of KRG-WE and GS markedly improved the number of germ cells, seminiferous tubular size, and Johnsen's score in the old rats. Ginseng produced a distinct testicular histological improvement in old rats. KRG-WE and GS elevated testosterone levels, while attenuating the aberrant increase in follicle stimulating hormone and luteinizing hormone levels. Sperm kinematics evaluated by a computer-assisted sperm analyzer demonstrated improvement in the percentage of motile sperm, progressive sperm motility, and curvilinear velocity associated with sperm quality, supporting the beneficial role of red ginseng in senile spermatogenesis. Overall, the total water extract had a more potent effect than the corresponding saponin fraction. In conclusion, Korean red ginseng rejuvenated age-induced testicular dysfunction. Additionally, the total water extract was more potent than the corresponding saponin fraction.

      • KCI등재

        Rapid detection of aneuploidy using FISH in uncultured amniocytes for prenatal diagnosis : 8-year experience

        Hwang, Do-Yeong,Lee, Dong-Suk,Choe, Jin,Choi, Hyeh-Sook,Min, Jeong-Yong,Lee, Soo-Min,Kim, Ki-Chul Korean Society of Medical Genetics 2007 대한의학유전학회지 Vol.4 No.2

        목 적:산전진단에 있어 빠른 진단을 위해 그 유용성이 널리 알려져 있는 FISH 방법을 미배양 세포에 적용할 때, 그 민감도를 높이기 위해 본 연구소의 경험과 기준을 소개하고자 한다. 방 법:1999년 5월부터 2006년 6월까지 본연구소에서 다운증후군 고위험군, 에드워드 증후군 고위험군, 고령산모, 초음파 이상소견 등의 적응증을 주소로 시행한 7,893례의 양수검체를 대상으로 빠른 진단을 위해 8,613례의 미배양 양수세포에 FISH 검사를 시행하였다. 분석은 함춘유전연구소의 기준에 따랐으며, 기존의 세포유전학적 결과와 최종 비교하였다. 결 과:8613례의 FISH 검사 결과, 30개 이상의 세포관찰이 가능하고, 정상인 경우 정상세포의 비율이 75%, 비정상의 경우 비정상 세포의 비율이 70%에 해당하는 8,502례의 결과를 얻었으며, 세포유전학적 결과와도 일치하였다. 결 론:산전진단 시 빠른 진단을 위한 FISH검사는 매우 유용하며, 정확한 분석을 위해 그 기준을 마련하는 것은 매우 중요하다 하겠다. 그러나 비용과 인력이 많이 소요되는 한계점을 가지고 있다. Purpose : FISH is suggested as a useful tool for rapid detection of specific aneuploidy in uncultured amniocytes abnormality in interphase nucleus. In this study, we are going to share our experience using FISH in prenatal diagnosis and suggest the criteria for the diagnosis of aneuploidy by analyzing the results of FISH test. Methods : From January, 1999 to May, 2006, 8,613 tests in amniotic fluids obtained from 7,893 pregnant women were performed by using FISH for prenatal diagnosis of trisomy 21, trisomy 18 and trisomy 13. The indications of chromosome study were a screen positive for Down syndrome or Edwards syndrome in maternal serum marker screening test and an advanced maternal age (${\geq}35$ years old). Results : We have the 8,502 informative results from 8,613 tests (98.7%) which is submitted our criteria and the sensitivity is 98.2%. Conclusion : FISH on uncultured amniocytes is a rapid, clinically useful tool for prenatal diagnosis, with informative specimens being highly accurate. But the limitation of FISH is both expensive and labor-intensive.

      • SCISCIESCOPUS

        Glucosamine exerts a neuroprotective effect via suppression of inflammation in rat brain ischemia/reperfusion injury

        Hwang, So-Young,Shin, Joo-Hyun,Hwang, Ji-Sun,Kim, Song-Yi,Shin, Jin-A,Oh, Eok-Soo,Oh, Seikwan,Kim, Jung-Bin,Lee, Ja-Kyung,Han, Inn-Oc Wiley Subscription Services, Inc., A Wiley Company 2010 Glia Vol.58 No.15

        <P>We investigated the neuroprotective effect of glucosamine (GlcN) in a rat middle cerebral artery occlusion model. At the highest dose used, intraperitoneal GlcN reduced infarct volume to 14.3% ± 7.4% that of untreated controls and afforded a reduction in motor impairment and neurological deficits. Neuroprotective effects were not reproduced by other amine sugars or acetylated-GlcN, and GlcN suppressed postischemic microglial activation. Moreover, GlcN suppressed lipopolysaccharide (LPS)-induced upregulation of proinflammatory mediators both in vivo and in culture systems using microglial or macrophage cells. The anti-inflammatory effects of GlcN were mainly attributable to its ability to inhibit nuclear factor kappaB (NF-κB) activation. GlcN inhibited LPS-induced nuclear translocation and DNA binding of p65 to both NF-κB consensus sequence and NF-κB binding sequence of inducible nitric oxide synthase promoter. In addition, we found that GlcN strongly repressed p65 transactivation in BV2 cells using Gal4-p65 chimeras system. P65 displayed increased O-GlcNAcylation in response to LPS; this effect was also reversed by GlcN. The LPS-induced increase in p65 O-GlcNAcylation was paralleled by an increase in interaction with O-GlcNAc transferase, which was reversed by GlcN. Finally, our results suggest that GlcN or its derivatives may serve as novel neuroprotective or anti-inflammatory agents. © 2010 Wiley-Liss, Inc.</P>

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