Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

Probing surface electronic properties of a patterned conductive STO by reactive ion etching

Jin, Mi-Jin,Choe, Daeseong,Lee, Seung Youb,Park, Jungmin,Jo, Junhyeon,Oh, Inseon,Kim, Shin-Ik,Baek, Seung-Hyub,Jeon, Cheolho,Yoo, Jung-Woo Elsevier 2019 APPLIED SURFACE SCIENCE - Vol.466 No.-

<P><B>Abstract</B></P> <P>SrTiO<SUB>3</SUB> (STO) is a highly attractive oxide material due to its flexible tunability of electrical properties. It can be designed to exhibit a high mobility with a tunable carrier concentration by creating oxygen vacancies, or by doping with Nb or La, which substitute the Ti and Sr sites, respectively. Here we show a micro-patterned surface doping of STO by using reactive ion etching (RIE). The creation and pattering of a conductive STO surface were achieved by sequential treatments with Ar and O<SUB>2</SUB> plasma. The patterned conductive surface edge was well defined as confirmed by an electrostatic force microscopy. The electronic characteristics of the RIE treated STO surface were probed by a synchrotron radiation photoemission spectroscopy, which shows the emergence of Ti<SUP>3+</SUP>, Ti<SUP>2+</SUP>, Ti<SUP>1+</SUP> states and metallic states near the Fermi level. The electrical mobility of the conductive STO surface can be increased up to 12000 cm/V s with a typical sheet carrier concentration around 10<SUP>13</SUP>–10<SUP>14</SUP> cm<SUP>−2</SUP>. Increasing Ar plasma time elongate the depth of the conductive surface, which reflects the change of magnetoresistance behavior at low temperature. The demonstrated control of the STO surface conductivity along with a large area and high precision patterning method can be widely used for a variety of oxide electronic and spintronic devices.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Development of simple micro/nano patterning process along with surface doping of STO. </LI> <LI> Revealing the change of electronic properties of STO according to Ar plasma time. </LI> <LI> Revealing transform from 2D- to 3D-like transport at STO surface according to Ar plasma time. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

KIOM-79에 의한 p65 단백질의 핵내 이동 및 iNOS 발현 억제

김진숙(Jin-Sook Kim),장대식(Dae-Sik Jang),전영진(Young-Jin Jeon),유호진(Ho-Jin You),박경한(Kyeong Han Park),김 명(Jin Ming),문형윤(Hyung-Yoon Moon),윤상필(Sang-Pil Yoon),장인엽(In-Youb Chang) 대한해부학회 2006 Anatomy & Cell Biology Vol.39 No.2

후박 (Magnolia officinalis), 갈근 (Pueraria lobata), 감초 (Glycyrrhiza uralensis) 및 대극(Euphorbia pekinensis)은 염증성질병을 포함한 다양한 질병을 치료하기 위해 사용되었다. 본 실험에서는 각각의 천연물의 복합추출물인 KIOM-79가 큰포식세포에서 inducible NO synthase (iNOS) 유전자의 발현을 억제시키는 기전을 밝혀내기 위하여 면역형광염색, RT-PCR, electrophoretic mobility shift assay (EMSA) 등을 실시하였다. 면역형광염색 소견을 보면 RAW 264.7 세포에 lipopolysaccharide (LPS)에 의해 유도된 iNOS 단백질의 발현이 KIOM-79에 의해 억제되는 것을 알 수 있다. Western blot과 RT-PCR 분석에 의하면 KIOM-79가 LPS에 의한 iNOS의 발현을 억제함을 확인 할 수 있다. LPS와 KIOM-79를 처리한 RAW 264.7 세포에서 nuclear extract를 추출하여 EMSA로 분석한 결과, LPS에 의해 유도된 NF-κB/Rel의 DNA 결합이 KIOM-79에 의해 억제됨을 확인하였다. 면역형광염색 소견을 보면 NF-κB/Rel의 구성단백질 중의 하나인 p65단백질은 세포질에서 발현되고 있으며, LPS를 처리하면 p65가 핵으로 이동함을 알 수 있다. 이때 KIOM-79를 처리하면 LPS에 의한 p65의 핵 내 이동이 억제됨을 확인할 수 있다. 결론적으로, KIOM-79는 큰포식세포에 작용해 iNOS 유전자의 발현을 억제하며, 이러한 큰포식세포의 활성억제는 p65 단백질의 핵 내 이동 억제를 통해서 유도되는 것으로 사료된다. We demonstrate that KIOM-79, combined extracts isolated from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of RAW 264.7 cells with KIOM-79 inhibited LPS-stimulated nitric oxide production in a doserelated manner. Immunohisto-chemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression. Immunostaining of p65 and EMSA showed that KIOM-79 inhibited NF-κ/Rel nuclear translocation and DNA binding, respectively. Collectively, this series of experiments indicates that KIOM inhibits iNOS gene expression by blocking NF-κ/Rel. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of KIOM-79 on iNOS suggest that KIOM-79 may represent a useful anti-inflammatory agent.

Interconnection 회로망의 시뮬레이션과 스윗칭

최진엽(Jin Youb Choi),김덕진(Duck Jin Kim),오길록(Kil Lok Oh) 대한전자공학회 1986 대한전자공학회 학술대회 Vol.1986 No.6

Repression of apurinic/apyrimidinic endonuclease by p53-dependent apoptosis in hydronephrosis-induced rat kidney

Chang, In Youb,Kim, Jin Nam,Jun, Jae Yeoul,You, Ho Jin,Jeon, Young Jin,Park, Kyeong-Soo,Yoon, Sang Pil Informa Healthcare 2011 Free radical research Vol.45 No.6

<P>p53 plays a major role in apoptosis through activation of pro-apoptotic gene Bax. It also regulates apurinic/apyrimidinic endonuclease (APE) expression in the base excision repair pathway against oxidative DNA damages. This study investigated whether p53-dependent apoptosis is correlated with APE using an experimental rat model of hydronephrosis. Hydronephrosis was induced by partial ligation of the right ureter. Animals were sacrificed on scheduled time after unilateral ureteral obstruction and the expression of 8-OHdG, γ款-H2AX, apoptotic proteins and APE was determined. The accumulated p53 activated Bax and caspase-3 7 days after hydronephrosis induction and the resulting high levels of p53-dependent apoptotic proteins and γ款-H2AX tended to decrease APE. The intensities of 8-OHdG and caspase-3 immunolocalization significantly increased in obstructed kidneys than in sham-operated kidneys, although APE immunoreactivity increased after hydronephrosis induction. These results suggest that oxidative DNA damages in obstructed kidneys may trigger p53-dependent apoptosis through repression of APE.</P>

Plasma leptin concentrations are greater in type II diabetic patients and stimulate monocyte chemotactic peptide-1 synthesis via the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway

( Jin Joo Cha ),( Young Youl Hyun ),( Yi Hwa Jee ),( Mi Jin Lee ),( Kum Hyun Han ),( Young Sun Kang ),( Sang Youb Han ),( Dae Ryong Cha ) 대한신장학회 2012 Kidney Research and Clinical Practice Vol.31 No.3

Background: Leptin is an adipokine that is recently reported to be a biomarker of systemic inflammation. Although atherosclerosis causes cardiovascular diseases, it is not clear whether leptin contributes to the acceleration of this process. In this study, we investigated whether alterations of plasma leptin levels were related to diabetic nephropathy and systemic inflammation. In addition, we examined the physiologic action of leptin in cultured vascular smooth muscle cells (VSMCs). Methods: A total of 126 type 2 diabetic participants and 37 healthy controls were studied. The diabetic participants were divided into three groups according to stage of nephropathy. We investigated whether leptin induced monocyte chemo- tactic peptide-1 (MCP-1) synthesis through the mitogen-activated protein kinase (MAPK) pathway using cultured VSMCs. Results: Plasma leptin concentrations were significantly higher in the diabetic group than in the controls. Plasma leptin levels were positively correlated with body mass index, fasting and postprandial blood glucose, hemoglobin A1c, total cholesterol, urinary albumin excretion, high-sensitivity C-reactive protein (hsCRP), and MCP-1 plasma levels, and negatively correlated with creatinine clearance values. In cultured VSMCs, leptin increased MCP-1 production in a dose-dependent manner, and this stimulating effect of leptin on MCP-1 expression was reversed by the MAPK (MEK) inhibitor PD98059. In addition, leptin stimulated the phosphorylation of MEK, extracellular signal-regulated kinase, and E26-like transcription factor, which are components of the MAPK pathway. Conclusions: Overall, these findings suggest that activation of leptin synthesis may promote MCP-1 activation in a diabetic environment via the MAPK pathway in VSMCs and that it possibly contributes to the acceleration of atherosclerosis.

Original Article : Heat Shock Proteins and Autophagy in Rats with Cerulein-Induced Acute Pancreatitis

( Jin Nam Kim ),( Hong Sik Lee ),( Soo Hyung Ryu ),( You Sun Kim ),( Jeong Seop Moon ),( Chang Duck Kim ),( In Youb Chang ),( Sang Pill Yoon ) 대한간학회 2011 Gut and Liver Vol.5 No.4

Background/Aims: Heat shock proteins (HSPs) protect rats from cerulein-induced acute pancreatitis (AP) by preventing the subcellular redistribution of cathepsin B and the activation of trypsinogen. Autophagy plays a critical role in the secretion of digestive enzymes and triggering of ceruleininduced AP via the colocalization of trypsinogen and lysosomes. Therefore, using a rat cerulein-induced AP model, we investigated whether HSPs prevent AP by regulating autophagy. Methods: Twelve hours after fed standard laboratory chow and water, the experimental groups (cerulein, water-immersion [WI]-cerulein and heat-shock [HS]-cerulein) and the control groups (control, WI, and HS) received one intraperitoneal injection of cerulein (50 μg/kg) or saline, respectively. All of the rats were sacrifi ced at 6 hours after injection. The severity of the AP was assessed based on the serum amylase level and the histological and electron microscopy findings. Western blotting was also performed for HSP60/70 and LC3B-II. Results: WI and HS induced HSP60 and HSP70, respectively. The induced HSP60/70 effectively prevented the development of cerulein-induced AP. Autophagy developed in the rats with cerulein-induced AP and was documented by the expression of LC3-II and electron microscopy fi ndings. The WI-stressed rats and HS-treated rats did not develop cerulein-induced autophagy. Conclusions: HSPs exert protective effects against cerulein-induced AP in rats by inhibiting autophagy. (Gut Liver 2011;5:513-520)

안구적출에 따른 위둔덕의 Calbindin D-28k과 c-fos의 변화에 관한 연구

김명,김기훈,김주영,하현철,안명수,김장민,조향훈,정명섭,장인엽 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Changes of Calbindin D-28k- and c-fos-immunoreactivities in the superior collicuclus after Eye Enucleation. Objective and methods: Calcium-binding proteins (CBPs) play an important role in the protection, differentiation and reorganization of the central nervous system. The effects of neonatal retinal deafferentation on a CBPs, calbindin D-28k were examined immunohistochemically in the superficial layer of the rat superior colliculus. Also early gene familly c-fos was examined to evaluate the neuronal characteristics in the superior colliculus after monocular enucleation. Results: On the experimental side of superior colliculus, the number of calbindin D-28k-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. Appearance of c-fos-like immunoreactivity was represented much more in the ipsilateral superior colliculus than contralateral side within 24h after eye enucleation. Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of calbindin D-28k and c-fos expression.

Poster Session : PS 1014 ; GI Motility : Clinical Outcomes of Early Gastric Cancer with Lymphovascular Invasion or Positive Vertical Margin after Endoscopic Submucosal Dissection

( Geum Youb Noh ),( Ha Ra Ku ),( Youn Joo Kim ),( Su Cheol Park ),( Jin Kim ),( Chul Ju Han ),( Yu Chul Kim ),( Ki Young Yang ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: In cases with lymphovascular invasion or positive vertical margins after endoscopic submucosal dissection(ESD) for early gastric cancer(EGC), additional radical gastrectomy is performed in principle. However, an additional surgery is diffi cult to consider if the surgical approach itself is challenging or the patient refuses surgery. In such cases, only close surveillance is performed, without additional surgical procedures. This study aimed to examine EGC cases with lymphovascular invasion or positive vertical margins after ESD. Methods: We retrospectively studied 83 patients with lymphovascular invasion or positive vertical margins after ESD from July 2005 to November 2013. Results: Of the 83 patients, 45 (54.2%) underwent radical additional gastrectomy (surgical group) and 38 (45.8%) were under close surveillance without surgical or endoscopic treatments (close surveillance group.) Cancer-free survival period was 78.3 ± 3.4 months in the surgical group and 64.5 ± 4.6 months in the close surveillance group. The recurrence rates did not signifi cantly differ between the 2 groups, at 7.9% in the surgical group and 6.7% in the non-surgical group. Conclusions: Close surveillance may be suggested as an option for EGC patients for whom surgical approach is diffi cult, who exhibit a positive vertical margin after ESD, and who have no lymphovascular or deep submucosal invasion after ESD.

스마트카드의 암호화모듈 구현에 적합한 Digit-Serial 유한체 연산기 설계

하진석(Jin-Suk Ha),이광엽(Kwang-Youb Lee),김원종(Won-Jong Kim),장준영(June-Young Chung),정교일(Kyo-Il Chung),배영환(Young-Hwan Bae) 대한전자공학회 2001 대한전자공학회 학술대회 Vol.2001 No.6