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Stemmed DNA nanostructure for the selective delivery of therapeutics
Jin, H.,Kim, M. G.,Ko, S. B.,Kim, D. H.,Lee, B. J.,Macgregor, Jr., R. B.,Shim, G.,Oh, Y. K. The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.16
<P>DNA has emerged as a biocompatible biomaterial that may be considered for various applications. Here, we report tumor cell-specific aptamer-modified DNA nanostructures for the specific recognition and delivery of therapeutic chemicals to cancer cells. Protein tyrosine kinase (PTK)7-specific DNA aptamer sequences were linked to 15 consecutive guanines. The resulting aptamer-modified product, AptG15, self-assembled into a Y-shaped structure. The presence of a G-quadruplex at AptG15 was confirmed by circular dichroism and Raman spectroscopy. The utility of AptG15 as a nanocarrier of therapeutics was tested by loading the photosensitizer, methylene blue (MB), to the G-quadruplex as a model drug. The generated MB-loaded AptG15 (MB/AptG15) showed specific and enhanced uptake to CCRF-CEM cells, which overexpress PTK7, compared with Ramos cells, which lack PTK7, or CCRF-CEM cells treated with a PTK7-specific siRNA. The therapeutic activity of MB/AptG15 was tested by triggering its photodynamic effects. Upon 660 nm light irradiation, MB/AptG15 showed greater reactive oxygen species generation and anticancer activity in PTK7-overexpressing cells compared to cells treated with MB alone, those treated with AptG15, and other comparison groups. AptG15 stemmed DNA nanostructures have significant potential for the cell-type-specific delivery of therapeutics, and possibly for the molecular imaging of target cells.</P>
Emodin accentuates atrial natriuretic peptide secretion in cardiac atria
Zhou, G.H.,Zhang, F.,Wang, X.N.,Kwon, O.J.,Kang, D.G.,Lee, H.S.,Jin, S.N.,Cho, K.W.,Wen, J.F. North-Holland ; Elsevier Science Ltd 2014 european journal of pharmacology Vol.735 No.-
Emodin, an active anthraquinone constituent isolated from the rhubarb, a traditional Chinese herbal medicine which is widely used in clinical treatment, has cardiovascular protective properties. However, it remains unclear whether the cardiovascular protective actions of emodin are related to an activation of cardiac natriuretic hormone secretion. The purpose of the present study was to explore the effect of emodin on the secretion of ANP, a member of the family of cardiac natriuretic hormones, and its mechanisms involved. Experiments were performed in isolated perfused beating rabbit atria allowing measurement of ANP secretion, atrial pulse pressure, and stroke volume. Emodin increased ANP secretion concomitantly with a decrease in atrial pulse pressure and stroke volume in a concentration-dependent manner. These effects were reversible. Inhibition of K<SUP>+</SUP> channels with tetraethylammonium and glibenclamide attenuated the emodin-induced changes in ANP secretion and atrial dynamics. Furthermore, the emodin-induced changes in ANP secretion and atrial dynamics were attenuated by inhibition of L-type Ca<SUP>2+</SUP> channels with nifedipine. Atropine, methoctramine, tertiapin-Q, and pertussis toxin had no significant effect on the emodin-induced changes in ANP secretion and mechanical dynamics. The present study demonstrates that emodin increases ANP secretion via inhibition of L-type Ca<SUP>2+</SUP> channels through an activation of K<SUP>+</SUP><SUB>ATP</SUB> channel in isolated beating rabbit atria. The results also provide a rationale for the use of emodin in the treatment of impairment of the regulation of the cardiovascular homeostasis.
재래돼지(Korea Native Pigs) 특이 genetic marker와 육질 연관성 분석
김철욱,여정수,조광근,진상근,오명곤,박준규,권은정,홍연희,김지현,이보경,박다혜,김재우,이지홍 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.6
본 연구는 재래돼지 특이적인 marker를 개발하고 이들 marker와 육질과의 연관성을 규명하기 위하여 실시하였다. 실험동물로 재래돼지와 랜드레이스 각각 30두씩 총 60두를 사용하였으며, 품종 특이 marker를 찾기 위하여 4번과 7번 염색체에 존재하는 총 60개의 microsatellite를 이용하여 PCR (polymerase chain reaction)을 수행하였다. PCR 증폭은 형광물질 Fam과 Hex, Ned로 표지된 한쌍의 microsatellite primer를 이용하였으며, 형광물질로 표식된 PCR 산물은 Genetic Analyzer ABI 310으로 전기영동하여 대립 유전자를 규명하고 통계 분석하였다. 대립 유전자에 대한 통계 분석 결과 총 60개의 microsatellite중 27개가 다양한 유전적 다형현상을 보이는 대립 유전자가 증폭되었으며 (p<0.05), 특히 SW1364와 SW445, SW1369, SWR773 microsatellite의 증폭 산물에서 재래돼지와 랜드레이스 두 품종간을 구분할 수 있는 품종 특이적인 대립 유전자를 확인하였다 (p<0.01). 또한 육질에 영향을 미치는 유전적 marker를 얻기 위해 60두 전체에 대한 육색, 지방색, 지방산 조성, 콜레스테롤 함량, 연도와 경도에 대한 육질 분석을 실시하여 육질 형질과 marker와의 연관성을 분석한 결과 육질 형질에 영향을 미치는 28개 marker를 확인하였으며, 육색과 지방색에 연관된 4개, 조직감과 연관된 10개의 marker를 확인하였다. 이렇게 선발된 marker들은 고급육 생산을 위한 돼지의 유전적 개량을 위하여 유용하게 이용될 수 있다. This study was conducted to develop Korean native pig-specific DNA markers and analyze its relativity with meat quality using 30 of Korean native pigs and Landraces, respectively. To find species-specific DNA markers, we selected and tested a total of 60 microsatellite markers on the 4 and 7 chromosome using polymerase chain reaction (PCR). PCR amplification was carried out by microsatellite primer pairs labeled with fluorescent Fam, Hex and Ned. The fluorescent labeled PCR products were electrophoresed with Genetic Analyzer ABI 310 followed by the allelic and statistical analysis. According to the allelic frequency annalysis, of the 60 microsatellite markers 27 were preduced with a variety of genetic polymorphic alleles (p<0.05). Especially, alleles of SW1364, SW445, SW1369 and SWR773 microsatellite markers were identified to be species-specific markers between two breeds (p<0.01). To analyze the meat quality we determined the meat color, fat color, fat composition, cholesterol contents and hardness, and compared these characteristics with the alleles of 36 species-specific markers in all pigs. Of the 60 microsatellite markers 28 were found to be related with meat quality; 18 for meat and fat color, 7 for fatty acid, 4 for cholesterol contents and 10 for shear force and hardness. These selected markers will be useful in the genetic improvement of pigs for an excellent meat quality.
박기배,이상호,김종원,진경태,손재익 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1
The objective of the project was the development of an optical approach which would indicate the presence of a flame and detect deviations from a desired set point. In this work, the flame quality indicator consisted of photodiode, optical fiber, interference filter and data acquisition system, and was tested in a lab-scale methane combustion system. It was needed to apply this system to pilot scale combustor in order to confirm whether temporal fluctuations might be correlated with important parameters of combustion process, such as fuel to air ratio and NO_(x) emissions or not.
Laser Raman Scattering 을 이용한 가스 분무내 당량비 계측에 관한 연구
박경석,진성호,송재익,김경수 한국액체미립화학회 1997 한국액체미립화학회지 Vol.2 No.4
Laser Raman scattering method has been applied to measure equivalence ratio of methane/air mixture in injected spray. We used high power KrF excimer laser(λ= 248nm) and a high gain ICCD camera to capture low intensity signal. Raman shifts and Raman scattering cross-sections of H₂, O₂, N₂, CO₂, CH₄ and C₃H_8 are measured precisely. Our results show an excellent agreement with those of other groups. Mole fraction measurement of O₂ and N₂ from air shows that O₂ : N₂ = 0.206 : 0.794. We used gas injector which was operated at 1 bar. Methane is used as a fuel. Spray region is l0mm × 37mm and this region is divided into 80 points. 100 Raman signals are obtained and ensemble averaged for each point. 3-d and contour plot of distribution of equivalenced ratio is presented. Our measured results show that the equivalence ratio of methane/air mixture in methane-rich region is reasonable. However, more study is necessary for methane-lean region because background noise level is almost same as Raman intensity of methane.
조광현,김인석,진영수,김재근 明知大學校 産業技術硏究所 1991 産業技術硏究所論文集 Vol.10 No.-
Two-phase flow phenomena are observed in many industrial facilities such as nuclear power plant and various heat exchangers. The experimental study has been investigated the classification of the flow pattern, the local void distribution and convective heat transfer in non-swirl two-phase flow under the isothermal and nonisothermal conditions.