자궁경부 상피종양에서 human papillomavirus 감염과 survivin 발현

윤재호,정동준,이정은,박동명,배동한,선우재근,백무준,김창진 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Human papillomavirus (HPV) has been considered a causative agent of uterine cervical carcinoma. HPV is a DNA oncogenic virus, which is well known as a causative virus in uterine cervical carcinoma. The virus is classified into two groups genotypically, low risk and high risk, according to the carcinogenic potentiality, and the determination of the viral genotype is important in clinical practice. Recently, numerous genotypes can be determined by high throughput method using DNA chip. Survivin is a recently characterized inhibitor of anti-apoptosis (IAP) protein, which is abundantly expressed in most solid and hematological malignancies, but undetectable in normal adult tissues. In this study, HPV genotypes are determined by DNA chip and the expression of survivin was examined by immunohistochemistry in 80 cases of uterine cervical intraepithelial neoplasia (CIN) and invasive carcinoma to see the roles of HPV and survivin in the carciogenesis of uterine cervical epithelial neoplasia. The results were as follows: 1. HPV positive rate was 72.5%, while negative rate was 27.5% in 80 cases of CIN and invasive carcinoma. The CIN and invasive carcinoma showed higher HPV positive rate (p <0.05). 2. HPV positive rate according to the histologic grade were 60%, 65%, 77% and 90% in CINI, CINII, CINII and invasive carcinoma, respectively. HPV positive rate showed increasing tendency according to the histologic grade, though there was no statistical significance. 3. The most frequent genotype was type 16 and the next were 58, 52, 18 and 33 in order of frequency. 4. Survivin was expressed in 96.3% of CIN and invasive carcinoma. The expression rate of survivin showed no significant difference between the histologic grade of CIN and invasive carcinoma, but showed tendency of increased expression rate in invasive carcinoma. 5. Survivin was expressed in HPV positive and in HPV negative each as in 95.5% and 96.6% respectively. There was no significant difference of survivin expression between HPV positive and negative cases. The above results suggest that HPV has no effect on the regulation of survivin expresson level in the uterine cervical intraepithelial neoplasia and invasive carcinomas.

Effects of gut microflora on pharmacokinetics of hesperidin: a study on non-antibiotic and pseudo-germ-free rats.

Jin, Ming Ji,Kim, Unyong,Kim, In Sook,Kim, Yuri,Kim, Dong-Hyun,Han, Sang Beom,Kim, Dong-Hyun,Kwon, Oh-Seung,Yoo, Hye Hyun Taylor Francis 2010 Journal of toxicology and environmental health. Pa Vol.73 No.21

<P>Hesperidin is a biologically active flavanone glycoside occurring abundantly in citrus fruits. In the present study, effects of intestinal microflora on pharmacokinetics of hesperidin were investigated using a pseudo-germ-free rat model treated with antibiotics. After administration of hesperidin to rats, hesperetin, hesperetin glucuronides, and metabolites postulated to be eriodictyol, hemoeriodictyol, and their glucuronides were detected in urine while hesperetin glucuronide was predominantly found in plasma. The plasma concentration-time profile of hesperetin was compared between non-antibiotic-exposed and pseudo-germ-free rats administered this compound. The maximal concentration (C(max)) values of hesperetin in non-antibiotic-exposed and pseudo-germ-free rats were 0.58 and 0.20 관g/ml, respectively, and area under the curve (AUC) values were 6.3 and 2.8 관g-h/ml, respectively. Thus, systemic exposure as evidenced by AUC and C(max) was significantly higher in normal compared to pseudo-germ-free rats. Fecal 관-glucosidase activities of non-antibiotic-exposed and pseudo-germ-free rats were 0.21 and 0.11 nmol/min/mg, while fecal 관-rhamnosidase activities were 0.37 and 0.12 nmol/min/mg, respectively. The rate of hesperidin transformation to hesperetin was 6.9 and 2.9 nmol/min/g in fecal samples in non-antibiotic-exposed and pseudo-germ-free rats, respectively. Taken together, these results showed that pharmacokinetic differences between non-antibiotic-exposed and pseudo-germ-free rats may be attributed to differing hesperidin uptake, as well as alterations in metabolic activities of intestinal flora.</P>

자연포기형 생물막공정에 의한 화학산업폐수 처리특성

김명희 ( Ming-ji Jin ),이순영 ( Soon-young Lee ),원찬희 ( Chan-hee Won ),곽규동 ( Gyu-dong Gwak ),엄진영 ( Jin-young Eom ),양기해 ( Gi-hae Yang ) 한국환경기술학회 2007 한국환경기술학회지 Vol.8 No.1

본 연구는 영세한 소규모 업체에서도 적은 비용부담으로 사용할 수 있는 오 · 폐수처리장치 개발이 필요한 실정에 맞추어 자연포기형 생물막공정을 이용한 난분해성물질을 포함한 고농도 화학산업 폐수의 유기물 용적부하와 수리학적부하에 따른 처리효율 분석을 통하여 자연포기형 생물막공정의 난분해성물질을 포함한 고농도 폐수 처리 효율성을 분석하고자 한다. 유기물 용적부하 0.2kg COD_Mn/㎥/d, 0.47kg COD_Mn/㎥/d, 0.78kg COD_Mn/㎥/d인 저율, 중율, 고율 살수여상의 운전범위에서 실험을 수행한 결과 유기물 용적부하 0.2kg COD_Mn/㎥/d, 0.47kg COD_Mn/㎥/d인 저율, 중율 살수여상의 운전범위에서 BOD₅, COD_Mn, COD_Cr 처리효율 모두 살수여상의 설계기준을 만족하며 안정적인 처리를 진행하였으나, 유기물 용적부하 0.78kg COD_Mn/㎥/d인 고율 살수여상의 운전범위에서 COD_Mn과 COD_Cr 처리효율은 설계기준에 다소 못 미치는 결과를 나타냈다. 수리학적부하 6.3㎥/㎡/d, 20.1㎥/㎡/d, 33.4㎥/㎡/d인 중속, 고속, 고속 살수여상의 수리학적부하 운전범위에서 실험을 수행한 결과 모든 운전조건에서 SS와 BOD5는 수리학적부하의 영향을 받지 않았으며, COD_Mn과 COD_Cr은 수리학적부하가 증가함에 따라 처리효율도 다소 증가하였으나 큰 영향은 받지 않았다. 따라서 경제적/효율적인 면을 동시에 만족시키기 위한 유기물 용적부하와 수리학적부하로 중율과 중속 살수여상의 운전범위에서 운전하는 것이 적정하다. In this study, natural aeration biofilm process was employed according to the requirement of development low cost-energy treatment equipment for small corporation. The efficiency of this equipment was evaluated at different organic loading rate and hydraulic loading rate to treat chemical wastewater which contains non-biodegradable matters. The chemical wastewater was treated under a trickling filter of organic loading rate at 0.2kgCOD_Mn/㎥/d, 0.47kgCOD_Mn/㎥/d and 0.78kg COD_Mn/㎥/d. when the organic loading rate is at low-rate of 0.2kg COD_Mn/㎥/d and intermediate-rate of 0.47kg COD_Mn/㎥/d, the removal efficiency of BOD₅, COD_Mn and COD_Cr can satisfied the design standard of general trickling filter process and the wastewater can be treated stably. However, when the organic loading rate is at high-rate of 0.78kg COD_Mn/㎥/d, removal efficiency of COD_Mn and COD_Cr can not satisfied the design standard of general trickling filter process. When the wastewater was treated at hydraulic loading rate of 6.3㎥/㎡/d, 20.1㎥/㎡/d and 33.4 ㎥/㎡/d, the removal efficiencies of SS and BOD₅ didn`t effected by hydraulic loading rate. While the removal efficiencies of COD_Mn and COD_Cr were increased by the increasing of by hydraulic loading rate, but the efficiencies were not distinct. To sum up, at the intermediate-rate of trickling filter, the effect of chemical wastewater treatment can be the economical and effective.

Kinetics of a Cloned Special Ginsenosidase Hydrolyzing 3-O-Glucoside of Multi-Protopanaxadiol-Type Ginsenosides, Named Ginsenosidase Type 3

( Xue Feng Jin ),( Hong Shan Yu ),( Dong Ming Wang ),( Ting Qiang Liu ),( Chun Ying Liu ),( Dong Shan An ),( Wan Taek Im ),( Song Gun Kim ),( Feng Xie Jin ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3

In this paper, the kinetics of a cloned special glucosidase, named ginsenosidase type III hydrolyzing 3-O-glucoside of multi-protopanaxadiol (PPD)-type ginsenosides, were investigated. The gene (bgpA) encoding this enzyme was cloned from a Terrabacter ginsenosidimutans strain and then expressed in E. coli cells. Ginsenosidase type III was able to hydrolyze 3-O-glucoside of multi-PPD-type ginsenosides. For instance, it was able to hydrolyze the 3- O-β-D-(1→2)-glucopyranosyl of Rb1 to gypenoside XVII, and then to further hydrolyze the 3-O-β-D-glucopyranosyl of gypenoside XVII to gypenoside LXXV. Similarly, the enzyme could hydrolyze the glucopyranosyls linked to the 3-O- position of Rb2, Rc, Rd, Rb3, and Rg3. With a larger enzyme reaction Km value, there was a slower enzyme reaction speed; and the larger the enzyme reaction Vmax value, the faster the enzyme reaction speed was. The Km values from small to large were 3.85 mM for Rc, 4.08 mM for Rb1, 8.85 mM for Rb3, 9.09 mM for Rb2, 9.70 mM for Rg3(S), 11.4 mM for Rd and 12.9 mM for F2; and Vmax value from large to small was 23.2 mM/h for Rc, 16.6 mM/h for Rb1, 14.6 mM/h for Rb3, 14.3 mM/h for Rb2, 1.81mM/h for Rg3(S), 1.40 mM/h for Rd, and 0.41 mM/h for F2. According to the Vmax and Km values of the ginsenosidase type III, the hydrolysis speed of these substrates by the enzyme was Rc>Rb1>Rb3>Rb2>Rg3(S)>Rd>F2 in order.

Pharmacokinetic Profile of Eight Phenolic Compounds and Their Conjugated Metabolites after Oral Administration of <i>Rhus verniciflua</i> Extracts in Rats

Jin, Ming Ji,Kim, In Sook,Park, Jong Suk,Dong, Mi-Sook,Na, Chun-Soo,Yoo, Hye Hyun American Chemical Society 2015 Journal of agricultural and food chemistry Vol.63 No.22

<P><I>Rhus verniciflua</I> (<I>Toxicodendron vernicifluum</I>) is a medicinal tree popularly used in Asian countries such as China, Japan, and Korea as a food additive or herbal medicine because of its beneficial effects. <I>R. verniciflua</I> extract (RVE) contains diverse phenolic compounds, such as flavonoids, as its major biological active constituents. In this study, the pharmacokinetic profiles of eight phenolic compounds were investigated following oral administration of RVE to rats. The eight phenolic compounds were 2,4-dihydroxybenzoic acid, 3,4-dihydroxybenzoic acid, fisetin, fustin, butin, sulfuretin, taxifolin, and garbanzol. The plasma concentrations of the eight compounds were determined by using a liquid chromatography–triple-quadrupole mass spectrometer before and after treatment with β-glucuronidase. When 1.5 g/kg RVE was administered, the eight compounds were all detected in plasma, mainly as conjugated forms. These pharmacokinetic data would be useful for understanding the pharmacological effects of RVE.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2015/jafcau.2015.63.issue-22/acs.jafc.5b01724/production/images/medium/jf-2015-01724m_0004.gif'></P>

A Novel Ginsenosidase from an Aspergillus Strain Hydrolyzing 6-O-Multi- Glycosides of Protopanaxatriol-Type Ginsenosides, Named Ginsenosidase Type IV

( Dong Ming Wang ),( Hong Shan Yu ),( Jian Guo Song ),( Yu Feng Xu ),( Chun Ying Liu ),( Feng Xie Jin ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.10

Herein, a novel ginsenosidase, named ginsenosidase type IV, hydrolyzing 6-O-multi-glycosides of protopanaxatrioltype ginsenosides (PPT), such as Re, R1, Rf, and Rg2, was isolated from the Aspergillus sp. 39g strain, purified, and characterized. Ginsenosidase type IV was able to hydrolyze the 6-O-α-L-(1→2)-rhamnoside of Re and the 6-O-β-D- (1→2)-xyloside of R1 into ginsenoside Rg1. Subsequently, it could hydrolyze the 6-O-β-D-glucoside of Rg1 into F1. Similarly, it was able to hydrolyze the 6-O-α-L-(1→2)- rhamnoside of Rg2 and the 6-O-β-D-(1→2)-glucoside of Rf into Rh1, and then further hydrolyze Rh1 into its aglycone. However, ginsenosidase type IV could not hydrolyze the 3-O- or 20-O-glycosides of protopanaxadioltype ginsenosides (PPD), such as Rb1, Rb2, Rb3, Rc, and Rd. These exhibited properties are significantly different from those of glycosidases described in Enzyme Nomenclature by the NC-IUBMB. The optimal temperature and pH for ginsenosidase type IV were 40℃ and 6.0, respectively. The activity of ginsenosidase type IV was slightly improved by the Mg2+ ion, and inhibited by Cu2+ and Fe2+ ions. The molecular mass of the enzyme, based on SDS-PAGE, was noted as being approximately 56 kDa.

Synthesis of 4H-pyrans catalyzed by thermol-regulated PEG1000-based ionic liquid/EM

Dong Fang,Jin-ming Yang,Hua-bin Zhang,Chang-mei Jiao 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3

A recyclable temperature-dependant phase-separation catalytic system comprised of PEG1000-based functional dicationic acidic ionic liquid and ethylene glycol monomethyl ether was used to prepare polyfunctionalized 4H-pyrans via one-pot three-component condensation with yields of 81–93%. The reaction was accomplished homogeneously at 90 8C, while the products was separated from the catalyst system by liquid/liquid phasic-separation at room-temperature, and the catalyst could be reused at least eight times without noticeably decreasing the catalytic activity.

Indoline-Based Molecular Engineering for Optimizing the Performance of Photoactive Thin Films

Ming Yu Jin,Byung-Man Kim,Hyun Sil Jung,Jun-Hyeok Park,Deok-Ho Roh,Dong Guk Nam,Tae-Hyuk Kwon,Do Hyun Ryu 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

Colossal grain growth yields single-crystal metal foils by contact-free annealing

Jin, Sunghwan,Huang, Ming,Kwon, Youngwoo,Zhang, Leining,Li, Bao-Wen,Oh, Sangjun,Dong, Jichen,Luo, Da,Biswal, Mandakini,Cunning, Benjamin V.,Bakharev, Pavel V.,Moon, Inyong,Yoo, Won Jong,Camacho-Mojica American Association for the Advancement of Scienc 2018 Science Vol.362 No.6418

<P><B>Turning many into one</B></P><P>Single-crystal metal foils are valuable for their surface properties that allow for synthesis of materials like graphene. Jin <I>et al.</I> present a strategy for creating colossal single-crystal metal foils called “contact-free annealing” (see the Perspective by Rollett). The method relies on hanging and heating commercially available, inexpensive, cold-rolled metal foils. Almost as if by magic, the polycrystalline grains rotate and anneal into a large single-crystal sheet with a specific crystal orientation. The strategy allows for the creation of much larger and much cheaper single-crystal metal foils.</P><P><I>Science</I>, this issue p. 1021; see also p. 996</P><P>Single-crystal metals have distinctive properties owing to the absence of grain boundaries and strong anisotropy. Commercial single-crystal metals are usually synthesized by bulk crystal growth or by deposition of thin films onto substrates, and they are expensive and small. We prepared extremely large single-crystal metal foils by “contact-free annealing” from commercial polycrystalline foils. The colossal grain growth (up to 32 square centimeters) is achieved by minimizing contact stresses, resulting in a preferred in-plane and out-of-plane crystal orientation, and is driven by surface energy minimization during the rotation of the crystal lattice followed by “consumption” of neighboring grains. Industrial-scale production of single-crystal metal foils is possible as a result of this discovery.</P>

Low Doses of Nonylphenol Promote Growth of Colon Cancer Cells through Activation of ERK1/2 via G Protein–Coupled Receptor 30

Ming Xie,Jin-Long Liang,Han-Dong Huang,Mai-Jian Wang,Tao Zhang,Xue-Feng Yang 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4

Purpose Nonylphenol (NP) is an endocrine disruptor found in products such as cleaners, plastics, and detergents. It exerts actions similar to endogenous 17-estradiol (E2) and is reported to influence various cancers. However, its role in colon cancer remains elusive. Materials and Methods Colon cancer cell lines COLO 205 and SW480 were employed in our study. The cells were treated with NP or E2 followed by measurement of apoptosis and proliferation using flow cytometry and MTT assays, respectively. G protein–coupled estrogen receptor 30 (GPR30) expression was visualized using immunofluorescence and Western blot. To investigate the underlying mechanism, the expression levels of GPR30, p-protein kinase A (PKA), c-myc, cyclin D1, and ERK1/2 were analyzed using Western blot. Meanwhile, the GPR30 antagonist G15 was utilized to validate the role of GPR30 in colon cancer progression. Finally, the effect of a GPR30 inhibitor on tumor growth was determined in vivo using tumor xenograft mouse models. Results NP facilitated the proliferation of colon cancer cells and induced apoptosis failure in vitro. Western blot revealed increased GPR30 expression levels in response to NP treatment. Cyclin D1, p-PKA, c-myc, and proliferating cell nuclear antigen, proteins that regulate the cell cycle, were all upregulated by NP, and NP-mediated ERK1/2 activation and subsequent cell proliferation were abrogated by the GPR30 inhibitor G15. Moreover, colon cancer mice that received G15 administration demonstrated impaired tumor growth in vivo. Conclusion Low dose NP promotes the growth of colon tumors through GPR30-mediated activation of ERK1/2 signaling.