MiR-124 regulates apoptosis in hypoxia-induced human brain microvessel endothelial cells through targeting Bim

Jie Pu,Yuan Long,Jian Zhou,Yanqiang Zhan,Xiaoyong Qin 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.6

Human brain microvessel endothelial cells (HBMECs) are crucial for brain vascular repair and maintenance. The high-expressed expressions of micro- RNA-124 (miR-124) in brain have been investigated and revealed in many researches. In this work, we aimed to investigate the role of miR-124 in apoptosis of HBMECs and the underlying mechanism. Here, we found the lowexpressed miR-124 in hypoxia-induced HBMECs using qRT-PCR analysis. MiR-124 targeting 30-untranslated region (30-UTR) of Bim mRNA was predicted by Targetscan database. Importantly, the decreased miR-124 expression and increased Bim expression, an opposite trend, were obtained in hypoxia-induced HBMECs. The further confirmation of the correlation between miR-124 and Bim was conducted by miR-124 overexpression and dual luciferase reporter assays. The inhibitory role of miR- 124 in Bim expression was evidenced by results obtained from miR-124 overexpression analysis. Luciferase reporter assay further proved that miR-124 directly targeted the two conserved seed sites in the Bim 30-UTR. The inhibited apoptosis of HBMECs was observed under both miR-124 overexpression and Bim knockdown condition in flow cytometry analysis. Collectively, these findings outline that miR-124 regulates apoptosis in hypoxia-induced HBMECs through targeting Bim, providing a better understanding of the role of miR-124 in apoptosis of HBMECs.

Three-dimensional Fe2O3–TiO2–graphene aerogel nanocomposites with enhanced adsorption and visible light-driven photocatalytic performance in the removal of RhB dyes

Jing-Jie Zhang,Pan Qi,Jie Li,Xiu-Cheng Zheng,Pu Liu,Xin-Xin Guan,Guang-Ping Zheng 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.61 No.-

A series of Fe2O3 and TiO2 co-doped graphene aerogel (Fe2O3–TiO2–GA) nanocomposites with a unique 3D architecture are assembled by a one-pot hydrothermal method combined with freeze-drying. The one with 30 wt% Fe2O3 exhibits the highest adsorptivity (95.0%) and the another with 25 wt% Fe2O3 displays the highest total efficiency (97.7%) including adsorption and visible light-driven photocatalytic degradation for rhodamine B dyes (20.0 mg L−1, 25 mL) within 1 h over 5.0 mg of the nanocomposites. The materials also display satisfactory cyclic stability and the removal efficiency can be 81.8% even after four runs. Additionally, a related photocatalytic mechanism is proposed.

Quantum Chemical Studies on Nicotinato Lead(II) Complex [Pb(II)(C5H4NCOO)2]

Pu Su Zhao*,Rong Qing Li,Jie Song,Meng Ping Guo 대한화학회 2008 Bulletin of the Korean Chemical Society Vol.29 No.3

The title compound of nicotinato lead(II) complex [Pb(C5H4NCOO)2] has been optimized at B3LYP/LANL2DZ and HF/LANL2DZ levels of theory. The calculated results show that the lead(II) ion adopts 2-coordinate geometry, which is the same as its crystal structure and different from the 4-coordinate geometry of isonicotinato lead(II) complex. Atomic charge distributions indicate that during forming the title compound, each nicotinic acid ion transfers their negative charges to central lead(II) ion. The electronic spectra calculated by B3LYP/LANL2DZ level show that there exist two absorption bands, which have some red shifts compared with those of isonicotinato lead(II) complex and the electronic transitions are mainly derived from intraligand π-π* transition and ligand-to-metal charge transfer (LMCT) transition. CIS-HF method is not suitable for the system studied here. The thermodynamic properties of the title compound at different temperatures have been calculated and corresponding relations between the properties and temperature have also been obtained. The second order optical nonlinearity was calculated, and the molecular hyperpolarizability was 1.147754 ´ 10^30 esu.

Synthesis, Crystal Structure, Spectra Characterization and DFT Studies on a Di-Cycle Pyrazoline Derivative

Jie Song,Pu Su Zhao,Guang Zhang 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.7

A dicycle pyrazoline derivative, 1-phenyl-5-(p-fluorophenyl)-3,4-(α-p-fluoro-tolylenecyclohexano) pyrazoline, was synthesized and characterized by elemental analysis, IR, UV-vis, fluorescence spectra and X-ray single crystal diffraction. Density function theory (DFT) calculations were performed by using B3LYP method with 6-311G** basis set. The optimized geometry can well simulate the molecular structure. Vibrational frequencies were predicted, assigned and compared with the experimental values, which suggest that B3LYP/6-311G** method can well predict the IR spectra. Both the experimental electronic absorption spectra and the predicted ones by B3LYP/6-311G** method reveal three electron-transition bands, with the theoretical ones having some red shifts compared with the experimental data. Natural bond orbital analyses indicate that the absorption bands are mainly derived from the contribution of n → π* and π → π*transitions. Fluorescence spectra determination shows that the title compound can emit blue-light at about 478 nm. On the basis of vibrational analysis, the thermodynamic properties of title compound at different temperature have been calculated,revealing the correlations between C0p, m, S0m, H0m and temperature.

Resource Allocation and Offloading Decisions of D2D Collaborative UAV-assisted MEC Systems

Jie Lu,Wenjiang Feng,Dan Pu 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.1

In this paper, we consider the resource allocation and offloading decisions of device-to-device (D2D) cooperative UAV-assisted mobile edge computing (MEC) system, where the device with task request is served by unmanned aerial vehicle (UAV) equipped with MEC server and D2D device with idle resources. On the one hand, to ensure the fairness of time-delay sensitive devices, when UAV computing resources are relatively sufficient, an optimization model is established to minimize the maximum delay of device computing tasks. The original non-convex objective problem is decomposed into two subproblems, and the suboptimal solution of the optimization problem is obtained by alternate iteration of two subproblems. On the other hand, when the device only needs to complete the task within a tolerable delay, we consider the offloading priorities of task to minimize UAV computing resources. Then we build the model of joint offloading decision and power allocation optimization. Through theoretical analysis based on KKT conditions, we elicit the relationship between the amount of computing task data and the optimal resource allocation. The simulation results show that the D2D cooperation scheme proposed in this paper is effective in reducing the completion delay of computing tasks and saving UAV computing resources.

Synthesis, Crystal Structure, Spectra Characterization and DFT Studies on a Di-Cycle Pyrazoline Derivative

Song, Jie,Zhao, Pu Su,Zhang, Wei Guang Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.7

A dicycle pyrazoline derivative, 1-phenyl-5-(p-fluorophenyl)-3,4-($\alpha$-p-fluoro-tolylenecyclohexano) pyrazoline, was synthesized and characterized by elemental analysis, IR, UV-vis, fluorescence spectra and X-ray single crystal diffraction. Density function theory (DFT) calculations were performed by using B3LYP method with 6-$311G^{**}$ basis set. The optimized geometry can well simulate the molecular structure. Vibrational frequencies were predicted, assigned and compared with the experimental values, which suggest that B3LYP/6-$311G^{**}$ method can well predict the IR spectra. Both the experimental electronic absorption spectra and the predicted ones by B3LYP/6-$311G^{**}$ method reveal three electron-transition bands, with the theoretical ones having some red shifts compared with the experimental data. Natural bond orbital analyses indicate that the absorption bands are mainly derived from the contribution of n $\rightarrow\pi^*$ and $\pi\rightarrow\pi^*$ transitions. Fluorescence spectra determination shows that the title compound can emit blue-light at about 478 nm. On the basis of vibrational analysis, the thermodynamic properties of title compound at different temperature have been calculated, revealing the correlations between $C^0_{p,m}$, $S^0_m$, $H^0_m$ and temperature.

Kinetics and activation energy of solvent swelling of coal altered by an ultrasonication-enhanced process

Zhaoting Pu,Jie Mi,Jian Kang,Shuaiguo Zhang 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.1

We studied the kinetics and activation energy of solvent swelling in Qinyuan and Xiongshan coal in per-chloroethylene (PCE) using conventional and ultrasonication-enhanced treatment conditions. Kinetic parameters sug-gested that both coal types exhibited anomalous diffusion regardless of treatment conditions. However, the kineticparameter n, an exponent that crudely indicates the nature of the solvent diffusion process, decreased with the addi-tion of ultrasound in both coal types. We suggest this change is due to the greater diffusion of PCE into coal duringultrasonication based on greater relaxation of the coal macromolecular network and microscopic agitation of the liq-uid solvent. Activation energy of coal solvent swelling decreased for both coal types with the addition of sonication,indicating that the energy barrier for the process was reduced in the presence of ultrasound.

Quantum Chemical Studies on Nicotinato Lead(II) Complex [Pb(II)(C₅H₄NCOO)₂]

Zhao, Pu Su,Li, Rong Qing,Song, Jie,Guo, Meng Ping Korean Chemical Society 2008 Bulletin of the Korean Chemical Society Vol.29 No.3

The title compound of nicotinato lead(II) complex [Pb$(C_5H_4NCOO)_2$] has been optimized at B3LYP/LANL2DZ and HF/LANL2DZ levels of theory. The calculated results show that the lead(II) ion adopts 2- coordinate geometry, which is the same as its crystal structure and different from the 4-coordinate geometry of isonicotinato lead(II) complex. Atomic charge distributions indicate that during forming the title compound, each nicotinic acid ion transfers their negative charges to central lead(II) ion. The electronic spectra calculated by B3LYP/LANL2DZ level show that there exist two absorption bands, which have some red shifts compared with those of isonicotinato lead(II) complex and the electronic transitions are mainly derived from intraligand $\pi$ -$\pi$ transition and ligand-to-metal charge transfer (LMCT) transition. CIS-HF method is not suitable for the system studied here. The thermodynamic properties of the title compound at different temperatures have been calculated and corresponding relations between the properties and temperature have also been obtained. The second order optical nonlinearity was calculated, and the molecular hyperpolarizability was $1.147754{\times}10^{-30}$ esu.

Application of adaptive local iterative filtering in axis trace purification of turbine generator rotor

Wenbin Zhang,Jie Min,Yun Wang,Yasong Pu,Jie Jiang,Dewei Guo,Libin Yu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.6

In order to solve the problem that the measured axis trace is polluted by noise and can not reflect the real fault type of the turbine generator rotor, the adaptive local iterative filtering is applied to the purification of the axis trace of the generator rotor. As an adaptive mode decomposition method, adaptive local iterative filtering can adaptively decompose a complex original signal into a series of single components reflecting the wave nature of the signal, then, by calculating the correlation coefficient between the autocorrelation function of each decomposition component and the autocorrelation function of the original signal, the real intrinsic mode function is extracted, finally, the extracted intrinsic mode function is used to synthesize the signal to purify the axis trace. The simulation analysis and the purification results of the measured axis trace show that the adaptive local iterative filter can effectively avoid the false components in the decomposition process and is more favorable for extracting the signal features by introducing the Fokker-Planck equation to design the filter, compared with ensemble empirical mode decomposition, this method has better purification performance.

Flexural behaviors of full-scale prestressed high-performance concrete box girders

Hongye Gou,Jie Gu,Zhiwen Ran,Yi Bao,Qianhui Pu 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.75 No.5

In this study, the flexural behaviors of full-scale prestressed concrete box girders are experimentally investigated. Four girders were fabricated using two types of concrete (compressive strengths: 50 MPa and 70 MPa) and tested under four-point bending until failure. The measured parameters included the deflection, the stress and strain in concrete and steel bars, and cracks in concrete. The measurement results were used to analyze the failure mode, load-bearing capacity, and deformability of each girder. A finite element model is established to simulate the flexural behaviors of the girders. The results show that the use of high-performance concrete and reasonable combination of prestressed tendons could improve the mechanical performance of the box girders, in terms of the crack resistance, load-carrying capacity, stress distribution, and ductility.