The Effects of Puerarin on CYP2D6 and CYP1A2 Activities In Vivo

Jiao Zheng,Bin Chen,Bing Jiang,Ling Zeng,Zhi-Rong Tang,Lan Fan,Hong-Hao Zhou 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.2

Ge-gen (Radix Puerariae) is used in traditional oriental medicine for various medicinal purposes. The drug is the root of a wild leguminous creeper, Pueraria lobata (Willd) Ohwi. It possesses a high content of avonoid derivatives, the most abundant of which is puerarin. Our goal was to find the effect of puerarin on cytochrome P450 enzymes in vivo. The study was conducted in 18 male volunteers of different genotypes (CYP2D6 *1/*1, *1/*10, *10/*10). Plasma was obtained at 6 h after oral administration and urine was collected from 0 to 8 h after probe drug administration. The logarithm value of metabolic rate decrease from -0.0055 ± 0.1887 to -0.1754 ± 0.2411 implied puerarin inhibited activity of CYP2D6. There was no significant relationship between the inhibition with the CYP2D6 genotypes. The paraxanthin/caffeine ratio in the plasma sample at 6th hour was increased by 30 ± 47% (p = 0.003), implied puerarin induced the activity of CYP1A2. While puerarin used together with the substrates of both enzymes, drug interaction worth the attention and at sometimes precautions are needed.

Transcriptome Analysis of Antrodia cinnamomea Mycelia from Different Wood Substrates

Jiao-Jiao Chen,Zhang Zhang,Yi Wang,Xiao-Long Yuan,Juan Wang,Yu-Ming Yang,Yuan Zheng 한국균학회 2023 Mycobiology Vol.51 No.1

Antrodia cinnamomea, an edible and medicinal fungus with significant economic value and application prospects, is rich in terpenoids, benzenoids, lignans, polysaccharides, and benzo- quinone, succinic and maleic derivatives. In this study, the transcriptome of A. cinnamomea cultured on the wood substrates of Cinnamomum glanduliferum (YZM), C. camphora (XZM), and C. kanehirae (NZM) was sequenced using the high-throughput sequencing technology Illumina HiSeq 2000, and the data were assembled by de novo strategy to obtain 78,729 Unigenes with an N50 of 4,463 bp. Compared with public databases, about 11,435, 6,947, and 5,994 Unigenes were annotated to the Non-Redundant (NR), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genome (KEGG), respectively. The comprehensive analysis of the mycelium terpene biosynthesis-related genes in A. cinnamomea revealed that the expression of acetyl-CoA acetyltransferase (AACT), acyl-CoA dehydrogenase (MCAD), 3- hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA), mevalonate pyrophosphate decarboxylase (MVD), and isopentenyl diphosphate isomerase (IDI) was significantly higher on NZM com- pared to the other two wood substrates. Similarly, the expression of geranylgeranyltransfer- ase (GGT) was significantly higher on YZM compared to NZM and XZM, and the expression of farnesyl transferase (FTase) was significantly higher on XZM. Furthermore, the expressions of 2,3-oxidized squalene cyclase (OCS), squalene synthase (SQS), and squalene epoxidase (SE) were significantly higher on NZM. Overall, this study provides a potential approach to explore the molecular regulation mechanism of terpenoid biosynthesis in A. cinnamomea.

The microwave magnetism of epitaxy LiFe5O8 thin film modulated by thickness

Jiao Yang,Jianfei Lei,Kai Du,Xudong Zheng,Xiujuan Jin 한국물리학회 2020 Current Applied Physics Vol.20 No.4

Spinel lithium ferrite LiFe5O8 (LFO) has attracted robust research interests due to their potential applications in isolators, circulators, and phase shifters. In this work, a series of LFO thin film with various thickness were fabricated on SrTiO3 (STO) single-crystalline substrates by pulsed laser deposition technology. We systematically investigated the influences of the thickness of LFO thin film on the crystal structure and magnetic properties. The in-plane lattice parameter a and in-plane lattice parameter c were modulated by controlling the thickness of LFO thin film, which was confirmed using reciprocal space mappings (RSMs) technology. Furthermore, the microwave magnetism of LFO thin film with various thickness were studied systematically by ferromagnetic resonance (FMR) measurement. Moreover, with increasing the thickness of LFO thin film from 50 to 180 nm, the difference of the ferromagnetic resonance field between easy- and hard-magnetization axis can be enhanced and reach to 330 Oe. These results illustrate that dynamic magnetic properties can be controlled by tuning the thickness of LFO thin film. Our work provides an effective method to tailor the lattice parameter and modify the magnetic properties of the LFO thin film and contributes to further design high-frequency functional device.

A Virtual-Queue based Backpressure Scheduling Algorithm for Heterogeneous Multi-Hop Wireless Networks

( Zhenzhen Jiao ),( Baoxian Zhang ),( Jun Zheng ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.12

Backpressure based scheduling has been considered as a promising technique for improving the throughput of a wide range of communication networks. However, this scheduling technique has not been well studied for heterogeneous wireless networks. In this paper, we propose a virtual-queue based backpressure scheduling (VQB) algorithm for heterogeneous multi-hop wireless networks. The VQB algorithm introduces a simple virtual queue for each flow at a node for backpressure scheduling, whose length depends on the cache size of the node. When calculating flow weights and making scheduling decisions, the length of a virtual queue is used instead of the length of a real queue. We theoretically prove that VQB is throughput-optimal. Simulation results show that the VQB algorithm significantly outperforms a classical backpressure scheduling algorithm in heterogeneous multi-hop wireless networks in terms of the packet delivery ratio, packet delivery time, and average sum of the queue lengths of all nodes per timeslot.

Microstructural and Mechanical Properties of TiCX–Ni3(Al,Ti)/Ni Functionally Graded Composites Fabricated from Ti3AlC2 and Ni Powders

Guangming Zheng,Zhenying Huang,Qun Yu,Wenqiang Hu,Xingyang Qiu,A. Lixia,Yuanbo Wang,Yidan Jiao,Yang Zhou,Hongxiang Zhai 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.6

In this paper, a novel type of functionally graded material (FGM) was successfully fabricated from pure Ni and Ti3AlC2powder mixtures by hot-press sintering route at 1200 °C and 17 MPa. In each layer, Ti3AlC2particles transformed into TiCxphase, while the additional Al–Ti atoms decomposed from Ti3AlC2diffused into the Ni matrix, giving rise to the formationof γ′-Ni3(Al,Ti). Part of the submicron TiCxand Ni3(Al,Ti) particles were uniformly distributed in the Ni matrix, whilesome of the TiCxmaintained the original Ti3AlC2plate-like morphology. Both TiCxand Ni3(Al,Ti) content decreased from60Ti3AlC2/Ni layer to 10Ti3AlC2/Ni layer in a stepwise manner was fabricated. The microstructural analysis indicatesthat no cracks, delamination and step-type abrupt changes occured in the graded layer of the manufactured FGM. Flexuralstrength and Vickers hardness enhanced with the increasing Ti3AlC2content except for fracture strain and toughness. Theoverall fracture toughness and the flexural strength of FGM can reach 21.76 MPa m1/2 and 1329 ± 34 MPa, respectively. Theprepared FGM, herein, with a hard ceramic surface on one side to resist local plastic deformation, and a ductile metallicsurface on the other side to provide toughness.

Experimental Study on the Mechanical Behaviour of Natural Loess Based on Suction-Controlled True Triaxial Tests

Fang Zheng,Shengjun Shao,Jiao Wang,Shuai Shao 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.8

Loess is mostly distributed in an unsaturated state in nature, and the complexity of the engineering properties of unsaturated soil is mainly due to the existence of matric suction. Therefore, matric suction must be considered in investigating the mechanical properties of unsaturated loess. However, soils are most often subjected to three principal stresses with different magnitudes in practical engineering. For the sake of examining and discussing the mechanical behaviour of unsaturated natural loess under a complex stress path, a suction-controlled true triaxial apparatus with a rigid-flexible boundary is used to test unsaturated natural loess under a complicated stress path. Four trials of isotropic consolidation tests are conducted on natural loess under suction-controlled conditions via the true triaxial apparatus. The consolidation yield characteristics of the natural loess under different matric suctions are investigated. Forty-eight trials of consolidated drained true triaxial tests under suction-controlled conditions are conducted on unsaturated natural loess to examine and discuss the influence of the matric suction and intermediate principal stress parameter (b-value). The consolidated drained trials are performed under a constant net mean stress and a constant matric suction with different intermediate principal stress parameters (b-values). Stress-strain curves and failure envelopes of the natural loess are also presented. The results indicate that the stress-strain-strength response of unsaturated natural loess depends on the matric suction and intermediate principal stress parameter under true triaxial conditions.

Anisotropic In-plane Resistivity and Magnetoresistance of the Detwinned BaFe2As2

Lin Jiao,Zong Fa Weng,Xue Yan Tang,Lu Kai Guo,Tian Shang,Lin Yang,Hui Qiu Yuan,Yu Ying Wu,Zheng Cai Xia 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.3

We have measured the electrical resistivity and the magnetoresistance of detwinned BaFe2As2single crystals in fields up to 16 Tesla. The temperature dependence of the resistivity shows acounterintuitive anisotropic behavior in the ab-plane, likely arising from the effect of the nematicsusceptibility. Little magnetoresistance is observed at temperatures above the structural/magnetictransition, below which a huge in-plane magnetoresistance with an anisotropy is detected.

Association of CAST Gene Polymorphisms with Carcass and Meat Quality Traits in Chinese Commercial Cattle Herds

Li, Jiao,Zhang, Lu-Pei,Gan, Qian-Fu,Li, Jun-Ya,Gao, Hui-Jiang,Yuan, Zheng-Rong,Gao, Xue,Chen, Jin-Bao,Xu, Shang-Zhong Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.11

Calpastatin (CAST), an endogenous inhibitor of the calpains, plays an important role in post-mortem tenderization of meat. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in the bovine CAST gene and association with carcass and meat quality traits. A total of 212 cattle from commercial herds were tested in this study including 2 pure introduced breeds, 4 cross populations, and 3 pure Chinese native breeds. Five SNPs were identified at position 2959 (A/G), 2870 (G/A), 3088 (C/T), 3029 (G/A) and 2857 (C/T) in the CAST gene (GenBank Accession No. AF159246). Allele frequencies of SNP2959 and SNP2870 were 0.701 (A) and 0.462 (A), respectively. A general linear model was used to evaluate the associations between the two markers and 7 traits. The results showed that both SNP2959 and SNP2870 were significantly (p<0.01) associated with the Warner-Bratzler shear force (WBSF), while they had no significant association with the other 6 traits in the whole population. However, in Chinese native pure breeds, only SNP2870 had significant association with WBSF (p<0.05). The simultaneous analysis of two-marker genotype effects indicated animals containing the A/G haplotype (A for SNP2959 and G for SNP2870) tended to have lower shear force than those containing the G/A haplotype, and, especially, animals homozygous for the A/G haplotype had approximately 2 kg lower shear force than those homozygous for the G/A haplotype (p<0.01). These results suggested that both markers may be effective for the marker-assisted selection of meat quality traits in Chinese commercial herds, especially SNP2870 which can be used for Chinese native cattle.

Growth and Differentiation Effects of Homer3 on a Leukemia Cell Line

Li, Zheng,Qiu, Hui-Ying,Jiao, Yang,Cen, Jian-Nong,Fu, Chun-Mei,Hu, Shao-Yan,Zhu, Ming-Qing,Wu, De-Pei,Qi, Xiao-Fei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

The Homer protein family, also known as the family of cytoplasmic scaffolding proteins, which include three subtypes (Homer1, Homer2, Homer3). Homer3 can regulate transcription and play a very important role in the differentiation and development for some tissues (e.g. muscle and nervous systems). The current studies showed that Homer3 abnormal expression changes in acute myeloid leukemia (AML). Forced expression of Homer3 in transfected K562 cells inhibited proliferation, influenced the cell cycle profile, affected apoptosis induced by $As_2O_3$ through inhibition of Bcl2 expression, and also promoted cell differentiation induced by 12-O-tetra decanoylphorbol-acetate (TPA). These results showed that Homer3 is a novel gene which plays a certain role in the occurrence and development of AML.

Semisupervised Particle Swarm Optimization for Classification

Zhang, Xiangrong,Jiao, Licheng,Paul, Anand,Yuan, Yongfu,Wei, Zhengli,Song, Qiang Hindawi Limited 2014 Mathematical problems in engineering Vol.2014 No.-

<P>A semisupervised classification method based on particle swarm optimization (PSO) is proposed. The semisupervised PSO simultaneously uses limited labeled samples and large amounts of unlabeled samples to find a collection of prototypes (or centroids) that are considered to precisely represent the patterns of the whole data, and then, in principle of the “nearest neighborhood,” the unlabeled data can be classified with the obtained prototypes. In order to validate the performance of the proposed method, we compare the classification accuracy of PSO classifier, k-nearest neighbor algorithm, and support vector machine on six UCI datasets, four typical artificial datasets, and the USPS handwritten dataset. Experimental results demonstrate that the proposed method has good performance even with very limited labeled samples due to the usage of both discriminant information provided by labeled samples and the structure information provided by unlabeled samples.</P>