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Jiao Yifan,Zhang Sihui,Yang Jing,Lai Xin-He,Dong Kui,Cheng Yanpeng,Xu Mingchao,Zhu Wentao,Lu Shan,Jin Dong,Pu Ji,Huang Ying,Liu Liyun,Wang Suping,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2
Two facultatively anaerobic, short rod-shaped, non-motile, Gram-stain-positive, unknown bacterial strains (JY-X040T and JY-X174) were isolated from fluvial sediments of Tongtian River in Yushu Tibetan Autonomous Prefecture, Qinghai province, China. Cells formed translucent, gray, round and convex colonies, with a diameter of less than 0.5 mm after 5 days of incubation at 30°C on brain heart infusion-5% sheep blood agar. The 16S rRNA gene sequence similarity between strain JY-X040T and Fudania jinshanensis 313T is 93.87%. In the four phylogenetic trees constructed based on the 16S rRNA gene and 423 core genes, the two isolates form an independent branch, phylogenetically closest to F. jinshanensis 313T, but could not be classified as a member of the genus Fudania or any other genus of the family Arcanobacteriaceae. The DNA G + C content of strain JY-X040T was 57.8%. Calculation results of average nucleotide identity, digital DNADNA hybridization value and amino acid identity between strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%, and 64.1%. The major cellular fatty acids were C16:0 (23%) and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α (L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and four unidentified components. The whole-cell sugars contained rhamnose and ribose. MK-10(H4) was the sole respiratory quinone. The minimum inhibitory concentration of streptomycin was 32 μg/ml. All physiological, biochemical, chemotaxonomic and genomic characteristics support that strains JY-X040T and JY-X174 represent members of a novel species in a new genus, Changpingibacter yushuensis gen. nov., sp. nov. The type strain is JY-X040T (GDMCC 1.1996T = KCTC 49514T).
Zhang, Jiao Jiao,Kang, Tae Yoon,Kwon, Taeho,Koh, Hyebin,Chandimali, Nisansala,Huynh, Do Luong,Wang, Xian Zhong,Kim, Nameun,Jeong, Dong Kee American Society for Microbiology 2019 Infection and immunity Vol.87 No.3
<P><I>Gallibacterium anatis</I> is a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity.</P><P><I>Gallibacterium anatis</I> is a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity. The objective of this study was to produce a specific chicken egg yolk antibody and evaluate its protective response against a <I>G. anatis</I> infection model in 4-week-old chicks. Enzyme-linked immunosorbent assays showed that hens immunized with the recombinant N terminus of <I>Gallibacterium</I> toxin A (GtxA-N) had significantly increased antibody titers against GtxA-N in serum and egg yolk IgY. Western blotting showed that IgY antibody had specificity against GtxA-N in the egg yolks of immunized hens. The growth of <I>G. anatis</I> in brain heart infusion (BHI) broth and agar was significantly inhibited by the GtxA-N-specific IgY antibody. The protective effects of the specific IgY antibody were evaluated in <I>G. anatis</I>-infected chicks after intramuscular injection (10 mg/ml). The anti-GtxA-N antibody titers in the sera of <I>G. anatis</I>-challenged chicks following an injection of specific IgY antibody were significantly higher than those of the control and the nonspecific IgY groups, but lower lesion scores for the peritoneum, liver, and duodenum were found after specific IgY antibody treatment. The results from this study suggest that the GtxA-N-specific IgY antibody could potentially improve the protective response against <I>G. anatis</I> infection in chicks.</P>
Synthesis of dioxanes via Prins reaction catalyzed by acyclic acidic ionic liquids
Dong Fang,Chang-mei Jiao,Hua-bin Zhang,Bao-hua Ji 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.2
Some recyclable acyclic SO3H-functionalized ionic liquids have been used as environmentally benign catalysts for the synthesis of dioxanes by Prins reaction. The products could simply be separated from the catalysts by decantation. After separation from the reaction mixture the catalysts could be recycled and reused for several times without a noticeable decrease in the catalytic activity.
Jiao Li,Pingsheng Huang,Longlong Chang,Xingwen Long,Anjie Dong,Jinjian Liu,Liping Chu,Fuqiang Hu,Jianfeng Liu,Liandong Deng 한국고분자학회 2013 Macromolecular Research Vol.21 No.12
A new platform of paclitaxel (PTX) for application as an oral delivery system was developed, by combining the pH sensitivity of polyelectrolyte complex nanoparticles (CNPs) and the active targeting of hyaluronic acid (HA). Chitosan/hyaluronic acid-paclitaxel (CS/HA-PTX) CNPs were prepared by coating the CS onto the HA-PTX nanoparticles (NPs), and characterized by Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1H NMR), transmission electron microscopy (TEM) and high-performance liquid chromatography (HPLC). HA-PTX conjugates could self-assemble into NPs in aqueous solution with an average size of 100±5 nm, and the PTX content of HA-PTX conjugates was 10.6 wt%. The CS/HA-PTX CNPs had a smaller size and higher PTX content when the ratio of positive charge to negative charge was 2:1. The in vitro release of PTX from CNPs was pH-responsive,suggesting that the CS shell could prevent the breakage of the ester bond in HA-PTX NPs in acidic pH conditions. HA-PTX NPs exhibited higher cellular uptake than free PTX against HepG2 cells via receptor-mediated endocytosis. PTX could accumulate remarkably into tumor sites after oral administration of CNPs. These results indicate that the CNP drug delivery system has great potential for applications in the oral administration of hydrophobic drugs.
Synthesis of 4H-pyrans catalyzed by thermol-regulated PEG1000-based ionic liquid/EM
Dong Fang,Jin-ming Yang,Hua-bin Zhang,Chang-mei Jiao 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3
A recyclable temperature-dependant phase-separation catalytic system comprised of PEG1000-based functional dicationic acidic ionic liquid and ethylene glycol monomethyl ether was used to prepare polyfunctionalized 4H-pyrans via one-pot three-component condensation with yields of 81–93%. The reaction was accomplished homogeneously at 90 8C, while the products was separated from the catalyst system by liquid/liquid phasic-separation at room-temperature, and the catalyst could be reused at least eight times without noticeably decreasing the catalytic activity.
Effects of Lubiprostone on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Colon
Jiao, Han-Yi,Kim, Dong Hyun,Ki, Jung Suk,Ryu, Kwon Ho,Choi, Seok,Jun, Jae Yeoul The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4
Lubiprostone is a chloride ($Cl^-$) channel activator derived from prostaglandin $E_1$ and used for managing constipation. In addition, lubiprostone affects the activity of gastrointestinal smooth muscles. Interstitial cells of Cajal (ICCs) are pacemaker cells that generate slow-wave activity in smooth muscles. We studied the effects of lubiprostone on the pacemaker potentials of colonic ICCs. We used the whole-cell patch-clamp technique to determine the pacemaker activity in cultured colonic ICCs obtained from mice. Lubiprostone hyperpolarized the membrane and inhibited the generation of pacemaker potentials. Prostanoid $EP_1$, $EP_2$, $EP_3$, and $EP_4$ antagonists (SC-19220, PF-04418948, 6-methoxypyridine-2-boronc acid N-phenyldiethanolamine ester, and GW627368, respectively) did not block the response to lubiprostone. L-NG-nitroarginine methyl ester (L-NAME, an inhibitor of nitric oxide synthase) and 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ, an inhibitor of guanylate cyclase) did not block the response to lubiprostone. In addition, tetraethylammonium (TEA, a voltage-dependent potassium [$K^+$] channel blocker) and apamin (a calcium [$Ca^{2+}$]-dependent $K^+$ channel blocker) did not block the response to lubiprostone. However, glibenclamide (an ATP-sensitive $K^+$ channel blocker) blocked the response to lubiprostone. Similar to lubiprostone, pinacidil (an opener of ATP-sensitive $K^+$ channel) hyperpolarized the membrane and inhibited the generation of pacemaker potentials, and these effects were inhibited by glibenclamide. These results suggest that lubiprostone can modulate the pacemaker potentials of colonic ICCs via activation of ATP-sensitive $K^+$ channel through a prostanoid EP receptor-independent mechanism.
Jiao Li,Dong Hu,Chao Tang,Jufang Xie 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.5
As a fuzzy set extension, the hesitant set is effectively used to model situations where it is allowable to determine several possible membership degrees of an element to a set due to the ambiguity between different values. Some new hesitant fuzzy operational rules are introduced based on the Hamacher t-conorm and t-norm, whereby we present a multi attribute decision making method under hesitant fuzzy information. In the daily management of library, book selection problem usually is very complex and unstructured, because variety of uncontrollable and unpredictable factors affects the evaluation and decision-making process at different levels. By integrating the multiple attribute decision making method with (Patron Driven Acquisitions, PDA) mode, a novel multiple attribute decision making framework based on Hamacher operations in PDA mode is proposed to select the best book providing the highest satisfaction for the attributes determined in this paper. Finally, a case study is so demonstrated to verify the reliability and applicability of the proposed framework.
Dong-Ju Chen,Li-Hua Yan,Qian Li,Cai-jiao Zhang,Chuan-Ling Si,Zhong-Yuan Li,Ya-Jian Song,Hao Zhou,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.6
Conjugated linoleic acid (CLA) isomers, c9, t11- CLA and t10, c12-CLA, have been proved to exhibit excellent biomedical properties for potential use in anticancer applications and in reducing obesity. Acer truncatum Bunge (ATB), which is rich in unsaturated fatty acids, including oleic acid, linoleic acid, and nervonic acid, is a new resource for edible oil. In the present study, we developed a new method for producing two CLA isomers from ATB-seed oil by fermentation using Lactobacillus plantarum CGMCC8198 (LP8198), a novel probiotics strain. Polymerase chain reaction results showed that there was a conserved linoleate isomerase (LIase) gene in LP8198, and its transcription could be induced by ATBseed oil. Analyses by gas chromatography–mass spectrometry showed that the concentration of c9, t11-CLA and t10, c12-CLA in ATB-seed oil could be increased by about 9- and 2.25-fold, respectively, after being fermented by LP8198.