Rapid Detection and Monitoring Therapeutic Efficacy of Mycobacterium tuberculosis Complex Using a Novel Real-Time Assay

( Jiang Li Juan ),( Wen Juan Wu ),( Hai Wu ),( Son Sik Ryang ),( Jian Zhou ),( Wei Wu ),( Tao Li ),( Jian Guo ),( Hong Hai Wang ),( Shui Hua Lu ),( Yao Li ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9

We combined real-time RT-PCR and real-time PCR (R/P) assays using a hydrolysis probe to detect Mycobacterium tuberculosis complex (MTBC)-specific 16S rRNA and its rRNA gene (rDNA). The assay was applied to 28 nonrespiratory and 207 respiratory specimens from 218 patients. Total nucleic acids (including RNA and DNA) were extracted from samples, and results were considered positive if the repeat RT-PCR threshold cycle was ≤35 and the ratio of real-time RT-PCR and real-time PCR load was ≥1.51. The results were compared with those from existing methods, including smear, culture, and real-time PCR. Following resolution of the discrepant results between R/P assay and culture, the overall sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) of all samples (including nonrespiratory and respiratory specimens) were 98.2%, 97.2%, 91.7%, and 99.4%, respectively, for R/P assay, and 83.9%, 89.9%, 72.3%, and 94.7%, respectively, for real-time PCR. Furthermore, the R/P assay of four patient samples showed a higher ratio before treatment than after several days of treatment. We conclude that the R/P assay is a rapid and accurate method for direct detection of MTBC, which can distinguish viable and nonviable MTBC, and thus may guide patient therapy and public health decisions.

EST-SSRs characterization and in-silico alignments with linkage map SSR loci in Grape (Vitis L.) genome

Hong Huang,Xia Xu,Xiaoqin Yang,Shuang Liang,Jiao Wu,Jiang Lu 한국유전학회 2012 Genes & Genomics Vol.34 No.1

11,581 grape (Vitis L.) EST-SSRs were produced and characterized from a total of 381,609 grape ESTs. Among the EST-SSRs, the tri repeat (5,560, 45.4%) represented the most abundant class of microsatellites in grape EST. Most of grape EST-SSR motifs fall within 18-24 bps in length. The EST-SSRs tri- repeats occurred a higher percentage in 5'-end (59.3%) than in 3'-end (48.3%). And EST-SSR tri- repeats had abundant codon repeats for putative amino acid runs as Proline, Arginine in grape ESTs. To better utilizing these markers, 142 of newly developed and validated EST SSR loci as well as 223 linkage map SSR loci were in silico aligned and mapped in grape genome. The orders of these SSR loci in the chromosomal physical locations and in the linkage groups were compared, and about twenty linkage map loci positions were switched or rearranged in grape genome. The EST-SSR markers extended the linkage map in grape genome. The method of in silico mapping reported in this study provided an initial collection for grape mapping resources. This approach offers great opportunities to understand the genetic variations in nucleotide sequences differences in physical map,and genetic recombination in linkage maps, as well as benefits for markers enrichment in a specific grape genome region for fine mapping or QTL mapping.

MiR-421 Regulates Apoptosis of BGC-823 Gastric Cancer Cells by Targeting Caspase-3

Wu, Jian-Hong,Yao, Yong-Liang,Gu, Tao,Wang, Ze-You,Pu, Xiong-Yong,Sun, Wang-Wei,Zhang, Xian,Jiang, Yi-Biao,Wang, Jian-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated in human gastric cancer. Here, we found that miR-421 was over-expressed in gastric cancer tissues and cell lines. Bioinformatics analysis predicted that the caspase-3 gene was a target of miR-421. Caspase-3 was negatively regulated by miR-421 at the post-transcriptional level. Bax and Bcl-2 were also regulated by miR-421. Moreover, tumor necrosis factor receptor-I and -II, death receptors in the apoptosis pathway, were up-regulated by miR-421. The over-expression of miR-421 promoted gastric cancer cell growth and inhibited apoptosis of the BGC-823 gastric cancer cell line. These observations indicate that miR-421 acts as a tumor promoter by targeting the caspase-3 gene and preventing apoptosis of gastric cancer cells through inhibition of caspase-3 expression. These findings contribute to our understanding of the functions of miR-421 in gastric cancer.

Associations between the rs6010620 Polymorphism in RTEL1 and Risk of Glioma: a Meta-analysis of 20,711 Participants

Wu, Yao,Tong, Xiang,Tang, Ling-Li,Zhou, Kai,Zhong, Chuan-Hong,Jiang, Shu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Background: Associations between the rs6010620 polymorphism in the regulator of telomere elongation helicase1 (RTEL1) gene and glioma have been widely reported but the results were not inconclusive. The aim of the current study was to investigate the association between the rs6010620 polymorphism in RTEL1 gene and risk of glioma by meta-analysis. Materials and Methods: We searched PubMed, Embase, Wanfang Weipu and CNKI (China National Knowledge Infrastructure) databases, which included all research published 05 May 2014. A total of 8,292 cases and 12,419 controls from 14 case-control studies involving the rs6010620 polymorphism in the RTEL1 gene were included. Statistical analysis was performed using STATA 12.0 software. Results: The results indicated that the rs6010620 polymorphism in RTEL1 gene was indeed associated with risk of glioma (OR=1.474, 95%CI=1.282-1.694, p<0.001). On subgroup analysis by ethnicity, we found associations between the rs6010620 polymorphism in the RTEL1 gene and risk of glioma in both Caucasians and Asians. Conclusions: The current meta-analysis suggested that the rs6010620 polymorphism in the RTEL1 gene might increase risk of glioma. In future, larger case-control studies are needed to confirm our results.

Molecular characterization and expression profiles of IscA1 gene in a longdistance migrant, Agrotis segetum

Hong Chang,Jiang Long Guo,Xiao Wei Fu,Xiu Jing Shen,Youming Hou,Kongming Wu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4

Cryptochrome (CRYs) proteins have been elucidated as the molecular basis for magnetoreception in Drosophila, and a putative magnetic receptor (named IscA1) protein may aslo be involved in sensing magnetic fields in Drosophila. However, whether IscA1 has a conserved role in diverse animals and functions in orientation during animal migration is unknown. Here we report on the cloning and sequencing of the IscA1 gene from Agrotis segetum, which encodes a predicted protein IscA1 that has 131 amino acids and two conserved iron-sulphur cluster binding domains. Multiple sequence alignment and phylogenetic analysis were used to show that IscA1 had a relatively high homology from species of Noctuoidea. Quantitative polymerase chain reaction showed that IscA1 was ubiquitously expressed in adult organs and, among all developmental stages, expression was higher in adults. When Agrotis segetum was exposed to 14 h light/10 h dark, IscA1 expression also showed daily oscillations, and constant light or dark disturbed these oscillations. IscA1 expression levels in a migratory population were higher than in a reared population and higher in a southward migratory population than in a northward. These findings suggest that the IscA1 gene in A. segetum might be associated with migration and provide a molecular basis for further study on the functions of IscA1 gene in magnetoreception and potential control of the turnip moth.

A flow rate on-line monitoring method for piezoelectric pump based on self-sensing circuit

Hong Jiang,Guohui Wu,Lipeng He,Yuecheng Wang,Dianbin Hu,Zheng Zhang,Guangming Cheng 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.5

This paper presents a new flow rate monitoring method for a piezoelectric micropump based on a self-sensing circuit. Utilizing the voltage generated by the secondary direct piezoelectric effect as the sensing voltage, a soft sensor of the piezoelectric actuator can be implemented onto the piezoelectric micropump. A bridge-type circuit is proposed as the selfsensing circuit, the piezoelectric micropump is fabricated with a circular piezoelectric actuator, the characteristics of the self-sensing voltage and the flow rate are experimentally investigated under different excitation voltages and frequencies. The results show that the method can accurately determine optimal frequency of the micropump (100 Hz, 280 Hz), and for a fixed frequency of 100 Hz and a voltage range of 50-180 V, the correlation between the self-sensing voltage and the flow rate is 0.9732. As a result, the method demonstrated in this paper allows precise monitoring of the flow rate of the piezoelectric micropump without using external sensors.

中國老齡化問題應對策略研究:集體社會資本視角下的老年人力資源開發

姜萱(Jiang, Xuan),陸競姸(Lu, Jing-yan),金紅花(Jin, Hong-hua),吳偉澤(Wu, Wei-ze) 동아인문학회 2021 동아인문학 Vol.57 No.-

인구의 고령화는 경제발전과 기술진보의 산물이자 삶의 질을 추구하는 인류의 필연적인 결과이다. 이 세계적인 난제를 해결하기 위해서는 인류 공통의 지혜가 필요하다. 중국은 2000년에 인구 고령화 국가 대열에 합류하여 현재는 거의 중등 고령화 수준에 도달했고, “부유해 지기 전의 고령화”, 급속한 고령화, 대규모 고령화의 특징을 보이고 있다. 인구 고령화는 미래 세계에서 영구적인 모습이 될 가능성이 높기 때문에 경제사회의 발전을 위해서는 노인인적자원 활용과 노인 수요 충족에 중점을 두어야 한다. 따라서 노인인적자원개발 사업이 중국의 현 인구 고령화 문제를 해결하는데 기여할 수 있을 것이라 판단된다. 본 연구는 Coleman의 집단의 사회적 자본 개념과 거시적 차원의 사회적 자본 이론을 기반으로 하였으며, 노인인적자원개발의 다자이익 집단은 사회개발, 조직개발, 자기개발의 세 가지 유형으로 구분하였다. 또한 중국의 고령화 현황과 노인인적자원개발의 전제 조건, 미래에 직면할 도전에 대하여 체계적으로 분석하고, 이를 바탕으로 최종적으로 중국의 실정에 맞는 인구 고령화 문제에 대한 해결책을 알아보았다. 본 연구의 목적은 다자이익집단이 함께 참여하여 사회적 자본을 모으고, 시너지 효과를 형성하여 인구 고령화 문제가 가져올 부정적인 결과를 완화하는데 있다. Population aging is the product of economic development and technological progress, as well as the inevitable result of human"s pursuit of high quality of life,which requires the common wisdom of human beings to solve this worldwide problem. China entered the ranks of population aging countries in 2000, and has now reached the aged society, and is characterized by “getting old before getting rich” problem, rapid population aging, and a large-scale elderly population. Since population aging has become an irreversible trend and is likely to become a permanent norm in the future all over the world, economic and social development needs to be closely promoted around the utilization of the elderly human resources and meeting their needs. Therefore, this paper advocates the development of the elderly human resources business as a means to solve the current problem of population aging in China. Based on Coleman"s concept of collective social capital and macro-level social capital theory, this paper classifies the multi-stakeholders in developing elderly human resources into three types: social development, organizational development and self-development. Through this, the paper makes a systematic analysis on the current situation of population aging in China, as well as the preconditions and future challenges of developing elderly human resources. Finally, the paper has constructed a set of solutions to the population aging problem in line with China"s national conditions. The purpose of this paper is to realize the use of multistakeholder groups to participate together, gather social capital and form a synergy to alleviate the adverse consequences of population aging. Findings are as follows: (1) in terms of social development, China should make full preparations under the national strategic framework by taking advantage of the “14th five-year plan” time window period, improve the policy system of elderly human resources development, improve the differences in the degree of aging in different regions according to local conditions, innovate ideas, and encourage high-quality elderly people to return to the labor market. (2) In terms of organizational development, it is necessary to improve the specialization and professionalism of the intermediary services of the elderly labor, break the organizational boundaries of communities. The paper suggests that educational organizations establish lifelong education and training systems, and that enterprises implement flexible management of the elderly human resources. (3) In terms of self-development, both the awareness of selfdevelopment and behavioral change of the elderly should be promoted. We should not only improve the awareness of re-employment and self-learning, but also making full use of the leading role of high-quality elderly human resources. So as to improve more elderly people to integrate into the elderly life with higher social value.

Correction to: Amelioration of radiation‑induced liver damage by p-coumaric acid in mice

Yun-Hong Li,Jiang-Xue Wu,Qian He,Jia Gu,Lin Zhang,Hao-Zhi Niu,Xin-Wen Zhang,Han-Ting Zhao,Jia-Ying Xu,Li-qiang Qin 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.5

In the original publication, incorrect versions of Figs. 2 , 3 ,4 and 5 were published. Specifi cally, the arrows in Figs. 2 , 3and 4 were moved outside the representative images, and theFig. 5 was wrongly replaced by another fi gure. The correctversion of Figs. 2 , 3 , 4 , and 5 , were shown below.

Amelioration of radiation-induced liver damage by p-coumaric acid in mice

Yun-Hong Li,Jiang-Xue Wu,Qian He,Jia Gu,Lin Zhang,Hao-Zhi Niu,Xin-Wen Zhang,Han-Ting Zhao,Jia-Ying Xu,Li-qiang Qin 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.10

Radiation-induced liver damage (RILD) is a spiny problem in radiotherapy or other circumstances that exposure to radiation. The need for radioprotective agent is increasing to protect liver tissue. This study aimed to explore the hepatoprotective effect of p-coumaric acid (CA) against RILD. C57BL/6 male mice were exposed to 4 Gy irradiation and administrated with CA for 4 days starting on the same day of irradiation. Mice were sacrificed to obtain blood and liver tissues on day 3.5 or 14 post irradiation, respectively. The blood and liver tissues were collected. As compared with the only irradiated group, CA supplementation improved liver morphology, decreased serum alanine aminotransferase and aspartate aminotransferase, inhibited BCL2-associated X (BAX) protein expression, and improved the mice hematopoietic function. CA at the dose of 100 mg/kg body weight showed better effect compared to the other doses. Thus, CA might possess potential to protect against RILD.

Surgical Management for Giant Liver Hemangiomas Greater Than 20 cm in Size

( Hui Jiang ),( Zhe Yu Chen ),( Pankaj Prasoon ),( Hong Wu ),( Yong Zeng ) 대한소화기기능성질환·운동학회 2011 Gut and Liver Vol.5 No.2

Background/Aims: The aim of this study was to investigate the primary management experience for giant liver hemangiomas greater than 20 cm in size. Methods: Records of patients referred for evaluation of radiologically and/or histopathologically proven giant liver hemangiomas between January 2007 and March 2010 were retrospectively analyzed. The reasons for referral, results of imaging studies, preoperative and surgical treatments, and outcome were reviewed. Results: A retrospective analysis was performed for 14 patients diagnosed with a giant hemangioma on the basis of an imaging study and/or a histopathological examination. All cases were diagnosed as giant liver hemangioma with at least one lesion greater than 20 cm in size. Abdominal discomfort was the main presenting complaint for the referral in 9 patients (64.2%). Abdominal ultrasound established the diagnosis in 12 patients (85.7%). Twelve patients underwent liver resection, 2 of whom underwent staged resection. Enucleation was performed in 2 patients. Selective transcatheter arterial embolization was implemented in 9 patients. Postoperative morbidity occurred in 3 patients (21.4%). No complications related to the hemangiomas occurred during follow up. Conclusions: Liver resection is indicated for giant liver hemangiomas with abdominal discomfort, especially for lesions greater than 20 cm in size. Staged operations are performed for patients with multiple lesions. Preoperative selective transcatheter arterial embolization alleviates progressive abdominal pain. (Gut Liver 2011;5:228-233)