Overexpression of Stromal Interaction Molecule 1/Store-operated Calcium Entry-associated Regulatory Factor in Interstitial Cells of Cajal in Mouse Jejunum Impairs Pacemaker Activity

( Jian-lin Lv ),( Ning-yuan Chen ),( Shang-ling Pan ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2018 Journal of Neurogastroenterology and Motility (JNM Vol.24 No.4

Genetic Linkage of Genes for a Thermo-sensitive Genic Male Sterility in Maize: The genetic analysis of an TGMS Line Qiong-6ms in maize

Zhi Yuan Fu,Hui Ling Xie,Jian Sheng Li,Yan Min Hu,Zong Hua Liu,Zhong You He,Ji Hua Tang 한국유전학회 2008 Genes & Genomics Vol.30 No.6

Thermo-sensitive genic male sterile (TGMS) lines can provide new options for hybrid seed production using "two-line" system. A set of F2 and BC1 populations derived from the cross between Qiong-6ms and Dan958 were employed to analyze the inheritance of a TGMS line Qiong-6ms and map the TGMS genes in maize. The results demonstrated that the sterility of Qiong-6ms was governed by two duplicative recessive genes, named tms1 and tms2. The gene tms1 was mapped to chromosome 5 linked with the SSR markers umc1355, umc2302 and umc1784 at a distance of 3.0 cM, 1.3 cM and 0.9 cM respectively; while tms2 was localized on chromosome 3, linked with SSR markers bnlg1605 (0.5 cM) and umc2050 (4.2 cM). These markers, which are tightly linked with the tms1 and tms2 genes, will be helpful for marker assisted selection of TGMS lines in maize.

Relation between Ki-67, ER, PR, Her2/neu, p21, EGFR, and TOP II-α Expression in Invasive Ductal Breast Cancer Patients and Correlations with Prognosis

Yan, Jian,Liu, Xiao-Long,Han, Lu-Zhe,Xiao, Gang,Li, Ning-Lei,Deng, Yi-Nan,Yin, Liang-Chun,Ling, Li-Juan,Yu, Xiao-Yuan,Tan, Can-Liang,Huang, Xiao-Ping,Liu, Li-Xin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

The aim of the present study was to investigate the expression of the transcription factor Ki-67, ER, PR, Her2/neu, p21, EGFR, and TOP II-${\alpha}$ in the tumor tissue of patients with invasive ductal carcinoma(IDC); in addition, we examined correlations between these markers. Two hundred and sixteen IDC patients, who were not previously been treated with chemo- or radiotherapy, were included in the study. All tumors were grade I-III. Expression of molecular markers was determined by immunohistochemical analysis on paraffin-embedded tissue sections. Follow-up data were collected for 3 months to 10 years and analyzed for tumor recurrence, survival time, and prognostic risk factors. We determined Ki-67 expression correlates with the expression of ER, PR, HER-2, EGFR, and TOP-${\alpha}$, as well as lymph node involvement, high tumor grade, lymphovascular invasion, high tumor stage, and high TNM stage in IDC. Positive Ki-67 expression was a risk factor for rapid tumor recurrence and may help tumor progression, leading to poor prognosis in IDC. Ki-67 was directly correlated with EGFR, TOP II-${\alpha}$, lymph node involvement, high tumor grade, lymphovascular invasion, high tumor stage, and high TNM stage in the hormone receptor subtypes of breast cancer. In triple negative breast cancer, Ki-67 correlated with TOP II-${\alpha}$. Expression of Ki-67 correlated with that of ER, PR, HER-2, EGFR, TOP II-${\alpha}$, and p21. In addition, the biomarker Ki-67 has a role as a prognostic factor and indicates a poor prognosis in IDC.

Metabolic engineering of Corynebacterium glutamicum for increasing the production of L-ornithine by increasing NADPH availability.

Jiang, Ling-Yan,Zhang, Yuan-Yuan,Li, Zhen,Liu, Jian-Zhong Published by Stockton Press on behalf of the Socie 2013 Journal of industrial microbiology & biotechnology Vol.40 No.10

<P>The experiments presented here were based on the conclusions of our previous proteomic analysis. Increasing the availability of glutamate by overexpression of the genes encoding enzymes in the L-ornithine biosynthesis pathway upstream of glutamate and disruption of speE, which encodes spermidine synthase, improved L-ornithine production by Corynebacterium glutamicum. Production of L-ornithine requires 2 moles of NADPH per mole of L-ornithine. Thus, the effect of NADPH availability on L-ornithine production was also investigated. Expression of Clostridium acetobutylicum gapC, which encodes NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, and Bacillus subtilis rocG, which encodes NAD-dependent glutamate dehydrogenase, led to an increase of L-ornithine concentration caused by greater availability of NADPH. Quantitative real-time PCR analysis demonstrates that the increased levels of NADPH resulted from the expression of the gapC or rocG gene rather than that of genes (gnd, icd, and ppnK) involved in NADPH biosynthesis. The resulting strain, C. glutamicum δAPRE::rocG, produced 14.84 g l?1 of L-ornithine. This strategy of overexpression of gapC and rocG will be useful for improving production of target compounds using NADPH as reducing equivalent within their synthetic pathways.</P>

Geophysical methods for the investigation of a closed dumping ground

Xin, Ling,Chu, Jian,Wang, Jing-Yuan,Yin, Ke,Tong, Huan-Huan,Chia, Charles Y.H.,Mohamed Noh, Omar A. Techno-Press 2015 Geomechanics & engineering Vol.8 No.5

Reclamation of closed dumping grounds is a potential solution to solve land scarce problems. Traditional geotechnical investigations of closed dumping grounds face some problems, such as the emission of hazardous liquids and gases, and the lack of ground information due to the discontinuity between two boreholes. Thus, noninvasive and continuous investigation methods are needed to supplement traditional geotechnical investigations. In this paper, two types of geophysical investigation methods, Seismic Analysis of Surface Waves (SASW) and 2D Resistivity, were carried out to study noninvasive and continuous site investigations for dumping grounds. The two geophysical methods are able to profile the distribution of physical properties of the fill and original materials, by which the extent of the dumping ground can be found and some anomalies in the subsurface can be located. Boreholes were used to assist in locating the dumping material-ground interfaces. The results show that dumping material-ground interfaces obtained from the two geophysical methods are roughly consistent. Moreover, attempt is made in the paper to use the geophysical methods to classify the types of dumping materials. The results show that the classification of dumping materials using the geophysical methods follows the results of the manual sorting of the dumping materials from a borehole.

Research on the Discrimination of Hatching Eggs Activity Based on Thermal Imaging : A Food Nondestructive Testing Practice

Liu Hai-ling,Cai Jian-rong,Sun Li,Yuan Lei-ming,Liu Meng-lei 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.2

The hatching eggs in 4-16 days incubation were from incubator of 38.5℃ to 22℃ for natural cooling and the time series images were acquired by thermal imaging technology. Analyzing the cooling curve and the cooling area and region growing, ellipse fitting, morphological processing and other methods were used to extract eggs region of interest; time series images superposition and contrast enhancement were used to raise the temperature close to the different categories of eggs gray differences; the ratio of gray value≦150 and entire ROI area was seen as characteristic parameters and the decision threshold was set according to minimum error rate Bayes rule. The non-fertilized eggs in incubating 4 days was 89.6% overall recognition and the dead embryo eggs in incubating 16 days was 96.3% overall recognition. Research showed that thermal imaging technology combined image processing technology can achieve non-destructive testing of hatching eggs activity.

The Classic Security Application in M2M: the Authentication Scheme of Mobile Payment

( Liang Hu ),( Ling Chi ),( Hong-tu Li ),( Wei Yuan ),( Yu Yu Sun ),( Jian-feng Chu ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.1

As one of the four basic technologies of IOT (Internet of Things), M2M technology whose advance could influence on the technology of Internet of Things has a rapid development. Mobile Payment is one of the most widespread applications in M2M. Due to applying wireless network in Mobile Payment, the security issues based on wireless network have to be solved. The technologies applied in solutions generally include two sorts, encryption mechanism and authentication mechanism, the focus in this paper is the authentication mechanism of Mobile Payment. In this paper, we consider that there are four vital things in the authentication mechanism of Mobile Payment: two-way authentication, re-authentication, roaming authentication and inside authentication. Two-way authentication is to make the mobile device and the center system trust each other, and two-way authentication is the foundation of the other three. Re-authentication is to re-establish the active communication after the mobile subscriber changes his point of attachment to the network. Inside authentication is to prevent the attacker from obtaining the privacy via attacking the mobile device if the attacker captures the mobile device. Roaming authentication is to prove the mobile subscriber`s legitimate identity to the foreign agency when he roams into a foreign place, and roaming authentication can be regarded as the integration of the above three. After making a simulation of our proposed authentication mechanism and analyzing the existed schemes, we summarize that the authentication mechanism based on the mentioned above in this paper and the encryption mechanism establish the integrate security framework of Mobile Payment together. This makes the parties of Mobile Payment apply the services which Mobile Payment provides credibly.

TLR9 Expression in Uterine Cervical Lesions of Uyghur Women Correlate with Cervical Cancer Progression and Selective Silencing of Human Papillomavirus 16 E6 and E7 Oncoproteins in Vitro

Hao, Yi,Yuan, Jian-Ling,Abudula, Abulizi,Hasimu, Axiangu,Kadeer, Nafeisha,Guo, Xia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Cervical cancer is listed as one of high-incidence endemic diseases in Xinjiang. Our study aimed to evaluate the expression of TLR9 in uterine cervical tissues of Uyghur women and examine associations with clinicopathological variables. We further characterized the direct effects of TLR9 upon the selective silencing of human papillomavirus (HPV) E6 and E7 oncoprotein expression in HPV 16-positive human cervical carcinoma cells treated with siRNA in vitro. Materials and Methods: Immunohistochemistry was applied to evaluate TLR9 expression in 97 formalin-fixed paraffin-embedded cervical samples from Uyghur women; 32 diagnosed with cervical squamous cell carcinomas (CSCC), 14 with low-grade cervical intraepithelial neoplasias (CINI), 10 medium-grade (CINII), 24 high-grade (CINIII), and 17 chronic cervicitis. $BLOCK-iT^{TM}$ U6 RNAi Entry Vector $pENTR^{TM}$/U6-E6 and E7 was constructed and transfected the entry clone directly into the mammalian cell line 293FT. Then the HPV 16-positive SiHa human cervical carcinoma cell line was infected with RNAi recombinant lentivirus. RT-PCR and Western blotting were used to determine the expression of TLR9 in both SiHa and HPV 16 E6 and E7 silenced SiHa cells. Results: Immunohistochemical staining showed that TLR9 expression was undetectable (88.2%) or weak (11.8%) in chronic cervicitis tissues. However, variable staining was observed in the basal layer of all normal endocervical glands. TLR9 expression, which was mainly observed as cytoplasmic staining, gradually increased in accordance with the histopathological grade in the following order: chronic cervicitis (2/17, 11.8%) <CINI (4/19, 28.6%) <CINII (3/10, 30.0%) <CINIII (12/24, 50.0%) <CSCC (17/32, 53.1%) (p<0.05), but not with tumor differentiation. RT-PCR and Western blotting showed that TLR9 expression was upregulated in HPV16 E6 and E7 silenced SiHa cells at both mRNA and protein levels. Conclusions: TLR9 expression increases according to the histopathological grade of cervical pathological process. HPV E6 and E7 oncoprotein have negative effects on the expression and function of TLR9.

Nanoparticle Realgar Powders Induce Apoptosis in U937 Cells through Caspase MAPK and Mitochondrial Pathways

Xiao-bo Wang,Hui-yuan Gao,Bai-ling Hou,Jian Huang,Rong-gang Xi,Li-jun Wu 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5

Nanoparticle realgar powders (NRP) inhibited U937 cell growth in a time and dose-dependent manner. U937 cells treated with NRP showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Caspase family inhibitor (z-VAD-fmk), caspase-8, -9 inhibitor (z-IETD-fmk, Ac-LEHD-CHO, respectively) and caspase-3 inhibitor (z- DEVD-fmk) partially prevented NRP -induced apoptosis. Moreover, the classical substrates of caspase-3, poly-ADP ribose polymerase (PARP) was degraded after U937 cells treatment with NRP. In addition, NRP increased the ratio of Bax/Bcl-2 protein expression. Although p38 inhibitor (SB203580) and ERK inhibitor (PD98059) failed to block cell death, JNK inhibitor (SP600125) had marked inhibitory effects on NRP -induced apoptosis. Furthermore, the phosphorylation of JNK was up-regulated, suggesting that JNK was responsible for NRP -induced apoptosis in U937 cells. These results suggested that the caspase, mitochondria and MAPK signal pathways were involved in NRP-induced U937 apoptosis.

Exosomes derived from miR-214-3p overexpressing mesenchymal stem cells promote myocardial repair

Wenwu Zhu,Qingjie Wang,Jian Zhang,Ling Sun,Xiu Hong,Wei Du,Rui Duan,Jianguang Jiang,Yuan Ji,Haoran Wang,Bing Han 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Aims Exosomes are known as nanovesicles that are naturally secreted, playing an essential role in stem-mediated cardioprotection. This study mainly focused on investigating if exosomes derived from miR-214 overexpressing mesenchymal stem cells (MSCs) show more valid cardioprotective ability in a rat model of acute myocardial infarction (AMI) and its potential mechanisms. Methods Exosomes were isolated from control MSCs (Ctrl-Exo) and miR-214 overexpressing MSCs (miR-214OE-Exo) and then they were delivered to cardiomyocytes and endothelial cells in vitro under hypoxia and serum deprivation (H/SD) condition or in vivo in an acutely infarcted Sprague-Dawley rat heart. Regulated genes and signal pathways by miR-214OE-Exo treatment were explored using western blot analysis and luciferase assay. Results in vitro , miR-214OE-Exo enhanced migration, tube-like formation in endothelial cells. In addition, miR-214OE-Exo ameliorated the survival of cardiomyocytes under H/SD. In the rat AMI model, compared to Ctrl-Exo, miR-214OE-Exo reduced myocardial apoptosis, and therefore reduced infarct size and improved cardiac function. Besides, miR-214OE-Exo accelerated angiogenesis in peri-infarct region. Mechanistically, we identified that exosomal miR-214-3p promoted cardiac repair via targeting PTEN and activating p-AKT signal pathway. Conclusion Exosomes derived from miR-214 overexpressing MSCs have greatly strengthened the therapeutic efficacy for treatment of AMI by promoting cardiomyocyte survival and endothelial cell function.