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Effect of human endogenous retrovirus-K env gene knockout on proliferation of ovarian cancer cells
Ko Eun-Ji,Kim Eun Taeg,Kim Heungyeol,Lee Chul Min,Koh Suk Bong,Eo Wan Kyu,Kim Hongbae,Oh Young Lim,Ock Mee Sun,Kim Ki Hyung,Cha Hee-Jae 한국유전학회 2022 Genes & Genomics Vol.44 No.9
Background: Among various human endogenous retroviruses (HERVs), the HERV-K (HML-2) group has been reported to be highly related to cancer. In pancreatic cancer cells, shRNA-mediated downregulation of HERV-K env RNA decreases cell proliferation and tumor growth through the RAS-ERK-RSK pathway; in colorectal cancer, CRISPR-Cas9 knockout (KO) of the HERV-K env gene affects tumorigenic characteristics through the nupr-1 gene. Objective: The effect of HERV-K env KO has not been studied in ovarian cancer cell lines. In this study, we analyzed the tumorigenic characteristics of ovarian cancer cell lines, including cell proliferation, migration, and invasion, and the expression patterns of related proteins after CRISPR-Cas9 KO of the HERV-K env gene. Methods: The HERV-K env gene KO was achieved using the CRISPR-Cas9 system in ovarian cancer cell lines SKOV3 and OVCAR3. Tumorigenic characteristics including cell proliferation, migration, and invasion were analyzed, and related protein expression was investigated by western blot analysis. Results: The expression of the HERV-K env gene in KO cells was significantly reduced at RNA and protein levels, and tumorigenic characteristics including cell proliferation, migration, and invasion were significantly reduced. In HERV-K env KO SKOV3 cells, the expression of the RB protein was significantly up-regulated and the cyclin B1 protein level was significantly reduced. In contrast, in HERV-K env KO OVCAR3 cells, the level of phospho-RB protein was significantly reduced, but other protein levels were not changed. Conclusion: The results of this study showed that HERV-K env gene KO affects cell proliferation, invasion, and migration of ovarian cells through RB and Cyclin B1 proteins, but the specific regulation pattern can differ by cell line.
Kim Do-Ye,Kim Heungyeol,Ko Eun-Ji,Koh Suk Bong,Kim Hongbae,Lee Ji Young,Lee Chul Min,Eo Wan Kyu,Kim Ki Hyung,Cha Hee-Jae 한국유전학회 2024 Genes & Genomics Vol.46 No.4
Background Human endogenous retrovirus (HERV)-K is a type of retrovirus that is present in the human genome, and its expression is usually silenced in healthy tissues. The precise mechanism by which HERV-K env influences cancer stemness is not fully understood, but it has been suggested that HERV-K env may activate various signaling pathways that promote stemness traits in cancer cells. Objective To establish the connection between HERV-K env expression and cancer stemness in ovarian cancer cells, we carried out correlation analyses between HERV-K env and the cancer stem cell (CSC) marker known as the cluster of differentiation 133 (CD133) gene in SKOV3 ovarian cancer cells. Method To perform correlation analysis between HERV-K env and CSCs, ovarian cancer cells were cultured in a medium designed for cancer stem cell induction. The expression of HERV-K env and CD133 genes was verified using quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot analyses. Additionally, the expression of stemness-related markers, such as OCT-4 and Nanog, was also confirmed using RT-qPCR. Results In the stem cell induction medium, the number of tumorsphere-type SKOV3 cells increased, and the expression of CD133 and HERV-K env genes was up-regulated. Additionally, other stemness-related markers like OCT-4 and Nanog also exhibited increased expression when cultured in the cancer stem cell induction medium. However, when HERV-K env knockout (KO) SKOV3 cells were cultured in the same cancer stem cell induction medium, there was a significant decrease in the number of tumorsphere-type cells compared to mock SKOV3 cells subjected to the same conditions. Furthermore, the expression of CD133, Nanog, and OCT-4 did not show a significant increase in HERV-K env KO SKOV3 cells compared to mock SKOV3 cells cultured in the same cancer stem cell induction medium. Conclusion These findings indicate that the expression of HERV-K env increased in SKOV3 cells when cultured in cancer stem cell induction media, and cancer stem cell induction was inhibited by KO of HERV-K env in SKOV3 cells. These results suggest a strong association between HERV-K env and stemness in SKOV3 ovarian cancer cells. Background Human endogenous retrovirus (HERV)-K is a type of retrovirus that is present in the human genome, and its expression is usually silenced in healthy tissues. The precise mechanism by which HERV-K env influences cancer stemness is not fully understood, but it has been suggested that HERV-K env may activate various signaling pathways that promote stemness traits in cancer cells. Objective To establish the connection between HERV-K env expression and cancer stemness in ovarian cancer cells, we carried out correlation analyses between HERV-K env and the cancer stem cell (CSC) marker known as the cluster of differentiation 133 (CD133) gene in SKOV3 ovarian cancer cells. Method To perform correlation analysis between HERV-K env and CSCs, ovarian cancer cells were cultured in a medium designed for cancer stem cell induction. The expression of HERV-K env and CD133 genes was verified using quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot analyses. Additionally, the expression of stemness-related markers, such as OCT-4 and Nanog, was also confirmed using RT-qPCR. Results In the stem cell induction medium, the number of tumorsphere-type SKOV3 cells increased, and the expression of CD133 and HERV-K env genes was up-regulated. Additionally, other stemness-related markers like OCT-4 and Nanog also exhibited increased expression when cultured in the cancer stem cell induction medium. However, when HERV-K env knockout (KO) SKOV3 cells were cultured in the same cancer stem cell induction medium, there was a significant decrease in the number of tumorsphere-type cells compared to mock SKOV3 cells subjected to the same conditions. Furthermore, the expression of CD133, Nanog, and OCT-4 did not show a significant increase in HERV-K env KO SKOV3 cells compared to mock SKOV3 cells cultured in the same cancer stem cell induction medium. Conclusion These findings indicate that the expression of HERV-K env increased in SKOV3 cells when cultured in cancer stem cell induction media, and cancer stem cell induction was inhibited by KO of HERV-K env in SKOV3 cells. These results suggest a strong association between HERV-K env and stemness in SKOV3 ovarian cancer cells.
Vi Capsular Polysaccharide(Vi CPS) 장티푸스 예방접종후 항체가의 변동 및 안전성
고대균,박지원,신완식,강문원,강진한 대한감염학회 1991 감염 Vol.23 No.3
Typhoid fever is still endemic in developing countries, and in many it poses a major public health problem. Although the antibiotic has improved prognosis and reduced mortality, persistence of mortality due to delayed introduction of treatment and appearance of resistant strains. It is therefore important to prevent this disease, and vaccination becomes the preferred means of prevention. Until now, the available vaccines, prepared from inactivated micro organisms, required several injections and caused serious adverse reactions. An oral vaccine composed of a live, attenuated strain is also available, but because repeated doses are required, its use in the fileld is of limited value. To evaluate the immunogenicity and safety of the Vi capsular polysaccharide (CPS) of Salmonella typhi, a single dose of 25㎍ of the Vi CPS was injected intramuscularly to 85 healthy children aged 5-15 years and 85 healthy young volunteers. None of these volunteers had previously suffered from typhoid fever and been vaccinated against typhoid fever. Adverse effects were assessed on day 0, 1, 2 and 3 according to clinical chart which was fulfilled by the subjects themselves. Anti-Vi antibody titers were measured by passive hemagglutination test before and 1, 3, and 12 months after vaccination. The side effects of Vi CPS were limited to minor and transient local reactions. Slight pain at the site of injection (47.1%), with erythema (4.7%) or induration (16.5%). Shivering was observed in only one case. The rate of seroconversion was 93.5% at 1 month (98.8% in children, 88.2% in adults). In eight of eleven non-seroconverters, the titers were increased four folds or more at 3 months. The antibody titers remained plateau for at least 12 months. To see whether this acquired level of Vi antibody titers protects vaccinees from typhoid fever, further clinical and epidemiological studies need to be performed.
제주계통의 STATCOM 상정사고를 고려한 전력품질 해석
고지한(Ko Ji-Han),김동완(Kim Dong-Wan),김승현(Kim Seong Hyun),김호민(Homin Kim),김일환(Kim Eel-Hwan) 한국태양에너지학회 2014 한국태양에너지학회 논문집 Vol.34 No.2
This paper presents the modeling and contingency analysis of Jeju power system. For the analysis of contingency with simulation, thermal power plants, current source type HVDC systems, wind farms, STATCOMs and Jeju power load are modeled by PSCAD/EMTDC program. And three kinds of simulation are carried out. Firstly, two STATCOMSs are in normal operation. Secondly, one STATCOM is in fault. Lastly, all of STATCOMs are in fault. These comparative studies will be useful for evaluating the effectiveness of STATCOM to stabilize for the Jeju power system.