고래회충 유충의 충체 및 분비 배설 단백질의 가수분해효소 활성 분석

옥미선 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Background and Objectives : Anisakis simplex L3 (third-stage larva) is the most prevalent species causing anisakidosis which is associated with consumption of raw or insufficiently processed fish or cephalopods. Adult worm of A. simplex inhabits in the stomach of cetaceans and pinnipeds such as whales, porpoises, and seals. Eggs, passed in the feces of the adult worm in marine mammals, hatch to liberate larvae, which then can be ingested by marine crustaceans. The third-stage larvae in the crustaceans are eaten by various species of fish or cephalopods of paratenic or transport hosts. Anisakidosis can cause not only direct tissue damage but also severe allergic response caused by larvae as well as by excretory-secretory (ES) products. The objectives of this study was to check the pattern of hydrolase activity from extracts of L3, L4 stages and ES products of A. simplex larvae. Materials and Methods : A. simplex L3 were collected from mackerels (Scomber japonicus). The third and fourth-stage larval extracts were obtained by homogenising and centrifugation. After A. simplex L3 were kept in PBS for 9 consecutive days, the supernatant was used as the excretory-secretory products. To identify the hydrolase activity in larval extract and ES products, SDS-PAGE and API ZYM method were carried out. Results : Six common positive enzymes were detected in L3, L4 and ES products belonging to four general groups as follows: (1) phosphatase group: alkaline phosphatase, acid phosphatase and naphthol-AS-BI-phosphohydrolase; (2) esterase group: esterase lipase (C8); (3) protease group: leucine arylamidase; and (4) glucosidase group: α-glucosidase. ES products was relatively rich in trypsin and α-chymotrypsin, apart from the common positive hydrolases. Conclusions : A. simplex L3, L4 extract and ES products contain several kinds of hydrolases of phosphatase, esterase, protease and glucosidase group, which appear to be important in larval development and host–-parasite interaction.

면역혈청학적 흡충류 검사법의 특이도 및 민감도 비교

옥미선 고신대학교 의학부 1992 高神大學校 醫學部 論文集 Vol.8 No.1

Double-Diffusion, Micro-ELISA, Dot-ELISA 등의 방법으로 3종의 흡출류, 간흡충, 폐흡충, 간질을 진단한 결과 특이도는 Double-Diffusion에서 높은 것으로 나타났으나 민감도는 떨어졌고, Micro와 Dot-ELISA는 민감도는 높았으나 특이도가 낮은 것으로 나타났다. To study the specificity and the sensitivity of immunodiagnostictests for 3 closely related species of trematodes, Clonorcbissinensis, Paragonimus westermani, Fasciola bepatica were used as antigens. Antisera of 13 clonorchiasis patients, 5 paragonimiasis patients, 1 fascioliasis patients, and 11 normal adults human sera were applied. Clonorchis antigen and antibody reaction in double-diffusion was weak, and only 23% among patients was positive. But paragonimiasis antisera-antigen reaction was very clear and 4 of 5 was positive. Clonorchiasis antisera-antisera was 100% positive in micro-ELISA, but clonorchis antigen, paragonimus antigen, fasciola antigen-fascioliasis antisera reaction was also 100% positive. In dot-ELISA, clonorchis antigen-antisera was 11 patients positive among 13 patients, but reaction of clonorchis antigen-paragonimus antisera was also 3 positive among 5 patients. Paragonimus antigen-clonorchis antibody never reacted, but 1 of 5 normal human sera reacted with clonorchis and paragonimus antigen. And fasciola antigen made cross-reaction with paragonimiasis patient sera and 10 of 13 clonorchiasis patient sera. In this experiments, although there was no cross-reactivity in double-diffusion, sensitivity was low. Micro and Dot-ELISA had high sensitivity, but specificity was low. And it was considered that the apprepriate mixture of 3 kinds of test will produce the good results.

폐결핵치료 중인 환자에서 Rifampicin에 의해 유발된 위막성 대장염 2예

김봉진,권균홍,임창섭,김자영,홍정범,옥미선,배용목,김지연 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Pseudomembranous colitis (PMC) is a disease caused by Clostridium difficile proliferation. The causative drugs are clindamycin, third-generation cephalosporins, flouroquinolone and so on. Rifampicin has been reported as a cause of PMC in the 1980s, and the frequency of PMC is increasing because rifampicin is a first line drug for anti-tuberculosis therapy. Two patients were recently admitted to our hospital due to watery diarrhea for 1 month and they were diagnosed with PMC by sigmoidoscopy. Their onset age were 74, 72 years old and latent period of symptoms were 60, 129 days, respectively. In one case, the patient displayed coexisting hypertension and diabetes. The clinical symptoms improved after discontinuing the rifampicin and then administering oral metronidazole. We report here on two cases presumed to be rifampicin-induced PMC.

Analysis of Expression Patterns of Thymosin β4 and CD133 in Normal Stomach

Mee Sun Ock(옥미선),Hee-Jae Cha(차희재) 한국생명과학회 2012 생명과학회지 Vol.22 No.10

Thymosin β4 는 대장암에서 암 줄기세포 마커인 CD133을 지닌 세포에서 지니지 않은 세포에 비해 강하게 발현된다고 보고되어 있다. 본 연구에서는 thymosin β4와 줄기세포 마커인 CD133의 상관관계를 정상 위 조직에서 관찰하였다. Thymosin β4와 CD133의 발현 양상은 tissue microarray 조직상에서 면역화학적 방법으로 관찰하였으며 thymosin β4와 CD133의 존재 위치는 면역형광 염색법 및 confocal microscope를 사용하여 조사하였다. Thymosin β4와 CD133은 동일한 양상으로 발현되었으며 모두 위의 선조직에서 강하게 발현되었다. 면역 형광 염색법으로 두가지 단백질을 동시에 염색한 결과 두 단백질이 동일한 위치에서 함께 존재하는 것으로 규명되었다. 이러한 결과는 thymosin β4와 CD133은 정상위의 선조직에서 발현되며 두 단백질의 발현 양상 및 위치가 동일하여 서로 긴밀한 상호작용을 할 가능성을 제시하고 있다. Thymosin β4 (Tβ4) has been reported to be overexpressed in CD133-positive colorectal cancer stem cells. We analyzed the relationship between Tβ4 and CD133-positive stem cells in normal stomach by examining the expression patterns of Tβ4 and CD133 in normal stomach tissues by immunohistochemical staining; co-localization of Tβ4 and CD133 was studied by immunofluorescence and confocal laser-scanning microscopy. Both Tβ4 and CD133 were expressed in stomach glands and showed similar expression patterns. Immunofluorescence staining of Tβ4 and CD133 showed that the expression of Tβ4 and CD133 was co-localized. In summary, both Tβ4 and CD133 were expressed in glands of normal stomachs and expression patterns were co-localized. These data suggest that Tβ4 expression is strongly related to CD133 expression.

Verifiable Hypotheses for Thymosin β4-Dependent and -Independent Angiogenic Induction of <i>Trichinella spiralis</i> -Triggered Nurse Cell Formation

Ock, Mee Sun,Cha, Hee-Jae,Choi, Yung Hyun Molecular Diversity Preservation International (MD 2013 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.14 No.12

<P><I>Trichinella spiralis</I> has been reported to induce angiogenesis for nutrient supply and waste disposal by the induction of the angiogenic molecule vascular endothelial cell growth factor (VEGF) during nurse cell formation. However, the action mechanism to induce VEGF in nurse cells by <I>T. spiralis</I> is not known. Hypoxia in nurse cells was suggested as a possible mechanism; however, the presence of hypoxic conditions in infected muscle or nurse cells and whether hypoxia indeed induces the expression of VEGF and subsequent angiogenesis in the infected muscle are both a matter of debate. Our recent studies have shown that thymosin β4, a potent VEGF inducing protein, is expressed in the very early stages of <I>T. spiralis</I> muscle infection suggesting the induction of VEGF in early stage nurse cells. Nevertheless, we now show that hypoxic conditions were not detected in any nurse cell stage but were detected only in the accumulated inflammatory cells. These studies propose that induction of angiogenesis by VEGF in <I>T. spiralis</I>-infected nurse cells was mediated by thymosin β4 and is unrelated to hypoxic conditions.</P>

THE EFFECTS OF MIIUNE RAT SERUM ON CLONORCHIS SINENSIS IN VITRO

Ock, Mee Sun 高神大學 醫學部 1988 高神大學醫學部 論文集 Vol.4 No.1

THE EFFECTS OF IMMUNE RAT SERUM ON CLONORCHIS SINENSIS IN VITRO

Ock, Mee-Sun 고신대학교 의학부 1988 高神大學校 醫學部 論文集 Vol.4 No.1

The present study was performed to clarify the effect of immune rat serum on Clonorchis sinensis, liver fluke, adult worms, excysted juvenile worms, metacercariae, and eggs in vitro. The adult and juvenile C. sinensis incubated in infected rat serum (IRS) developed tegumental precipitate which seemed to have an effect on the viability of parasites. No such reactions were observed in normal rat serum (NRS). Metacercariae and eggs cultured in immune serum did not show the precipitate on tegument. Heat inactivation of IRS at 56℃, 30 min, diminished the precipitate dramatically on adult worms. But the ability of precipitate formation against adult worms regained upon addition of NRS. From the results of the present study, it was suggested that the precipitate on tegument and in the medium have parasiticidal effects on C. sinensis adults and young worms, and is a specific immunoglobulin(s) and complement-dependent.

Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen

Mee Sun OCK,Bong Jin KIM,Sun Mi KIM,Kang Hyun BYUN 대한기생충학열대의학회 2005 The Korean Journal of Parasitology Vol.43 No.3

악성 단계별 인간 전립선 암세포에서 라미닌에 의한 metallothionein 유전자 발현유도 현상 연구

Mee Sun Ock(옥미선),Hee-Jae Cha(차희재) 한국생명과학회 2011 생명과학회지 Vol.21 No.4

라미닌-1에 의해 분화가 유도되는 것으로 알려져 있는 HSG 및 PC-12에서는 라미닌-1에 의해 MT 유전자의 발현이 유도되었지만 반면 분화 역량을 지니지 않은 암세포인 유방암(MDA-231, MDA-435) 세포와 전립선 암인 PC-3 세포에서는 라미닌-1의 처리가 MT 유전자의 변화에 영향을 미치지 못하는 것이 관찰되었다. 라미닌-1에 의해 분화가 유도되는 현상 및 이에 따른 MT 유전자의 발현증가가 암의 전이 능력 및 악성화와 관계가 있는지를 관찰하기 위해 정상에서부터 전이 및 악성 정도가 다른 5가지 종류의 전립선 암을 대상으로 라미닌-1에 의한 MT 유전자의 발현 변화를 관찰한 결과 정상적인 전립선 외피세포인 RWPE-1과 전이 및 악성화가 낮은 WPE1-NA22의 경우 라미닌-1에 의해 MT 유전자의 발현이 증가하였으며, 악성화 정도가 높은 WPE1-NB14, WPE1-NB11, 및 WPE1-NB26에서는 라미닌-1의 처리에도 MT 유전자의 발현이 증가하지 않는 것이 관찰되었다. 이러한 결과를 통해 라미닌-1은 정상 세포의 분화를 유도하며 이에 따라 MT 유전자를 유도하며 분화가 유도되지 않는 악성 암에서는 MT 유전자의 발현이 유도되지 않는 것으로 확인되었다. Metallothioneins (MT) are a group of low-molecular weight, cysteine-rich, intracellular proteins that are encoded by a family of genes containing at least 10 functional isoforms in human. The expression and induction of these proteins is associated with protection against DNA damage, oxidative stress, and apoptosis. Many studies have shown increased expression of MT in various human tumors, whereas MT is down-regulated in certain tumors such as hepatocellular carcinoma and liver adenocarcinoma. Hence, the expression of MT is not universal to all human tumors but may depend on the differentiation status and proliferative index of tumors, along with other tissue factors and gene mutations. Using Northern blot analysis, we found that laminin induced expression of MT-1 in HSG and PC12 cells, which can be differentiated by laminin, but had no effect on MB-231, MDA-435, and PC-3 cells, which cannot be differentiated by laminin. In addition, we analyzed the expression level of the MT-1 gene in five prostate cancer cell lines possessing different metastatic potential. The expression of MT-1 in normal and less malignant cells (RWPE-1 and WPE1-NA22) was high and up-regulated by laminin, whereas the expression of MT-1 in WPE1-NB14, WPE1-NB11, and WPE1-NB26 cells (malignant) was extremely low and not elevated by laminin. These results suggest that the MT-1 gene is involved in laminin-mediated differentiation and affects the metastatic potential of tumor cells.

On the Alimentary Canal of Larvae and Adults of Anax parthenope (Odonata, Anisoptera)

OCK, MEE SUN,LEE, CHANG EON 韓國自然史博物學會 1981 生物과 自然 Vol.11 No.2

왕잠자리 幼蟲과 1日경과한 成蟲의 消化管 組織을 比較 觀察하였다. 前腸은 비교적 긴 관이며 한층의 편평상피 세포층과 불규칙한 내층 그리고 얇은 근육층으로 구성된다. 성충도 이와 유사하나 길이가 길어짐으로써 中腸 가까이에서 한번 꼬여 있다. 위맹낭은 전장쪽으로 짧게 뻗어있고 유충에서는 낭의 내측 세포층만 주름져 있으나, 성충에서는 囊의 內外側 세포층으로 모두 주름져 있었다. 유충의 중장은 圓柱上皮細胞와 再生細胞로 이루어지며 변태의 일환으로 원주상피의 중강 부분이 떨어져나와 內腔에 흩어져 있었다. 성충의 중장은 원주상피로 구성되며 세포층은 완전히 주름잡혀져 있고 분비작용이 왕성하다. 특히, 胃冒囊 주위와 後腸근처에서 더욱 활발하다. Anax.parthenope는 유충시기에는 水棲이므로 호흡기관으로 직장새가 발달되었다. 이 직장새는 6개의 쌍을 이운 새정으로 구성되며, 각 새정은 마주 대하고 있는 Pad에서 발생한다. 새정은 심한 분지현상을 보인다. 성충이 되면 직장새가 사라지고 전형적인 6개의 乳頭突起가 나타나나 그중 3개의 돌기를 구성하는 세포는 비교적 크고 나머지 3개의 돌기를 구성하는 세포는 작게 나타난다. The alimentary canal of A. parthenope was observed with light microscope. The preparations was made by paraffin method and stained with H·E and others. There is little difference in the foregut during the migration of a habitat concurrent with the metamorphosis. The midgut shows very active secretion as a result of growth. The larval ileum has very characteristic structure which is similar to the cryptonephric system in Diptera. It is considered that this struc ture is substituted the function of the rectum which serves as the respiratory organ. The rectum proves the consequence of the migration of a habitat. The rectal gills needed to respiration of aquatic larvac disappear and typical six papillae come out.