Anti-bacterial Effects of Non-thermal Plasma Water on Acne Vulgaris

Jesmin Ara,Dong Heui Kim,Kyu Jae Lee 한국물학회 2017 한국물학회지 Vol.6 No.1

Human skin is full of normal flora, most of the flora has a beneficial effect on human skin. In certain condition, cutaneous flora can trigger pathologic conditions like acne vulgaris. Propionibacterium acnes and Staphylococcus epidermidis were recognized as pus-forming bacteria triggering an inflammation causing acne. The present study conducted to evaluate antibacterial activities of non- thermal plasma water against these etiologic agents of acne vularis. we tested both aerobic and anaerobic load of facial bacteria especially from cheek and forehead before and after application of non-thermal plasma water. These results shows effective antibacterial effect on both aerobic and anaerobic bacteria from all the participants. Therefore, this plasma water is recognizable in functional and could be a possible alternative treatment for acne.

The immunological and oxidative stress regulation of non-thermal plasma-aided water on atopic dermatitis-like lesion in dinitrochlorobenzene-induced SKH-1 hairless mice

Jesmin Ara,Johny Bajgai,Ma. Easter Joy Sajo,Ailyn Fadriquela,Cheol-Su Kim,Soo-Ki Kim,Kyu-Jae Lee 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.2

Backgrounds: While non-thermal plasma aided water (NTP-AW) has been currently used as a novel therapeutic against wide array of diseases, it remains unknown on therapeutic effect against atopic dermatitis (AD). To answer this, we evaluated the effect of NTP-AW on the regulation of immune and antioxidant markers in dinitrochlorobenzene (DNCB)-induced AD in hairless mice. Methods: The skin damage in mice was induced by 1% DNCB for the first wk and 0.5% every alternate day for 3 wks followed by 4 wks NTP-AW treatment through spraying and bathing method. Results: Treatment with NTP-AW reduced the severity of dermatitis symptoms and skin scratching behavior. In line, immunological profiling showed the improved parameters such as decrease in the number of neutrophils, lymphocyte, eosinophil as well as Th2-mediated IgE overproduction, and the reduced level of pro-inflammatory cytokine such as interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. Redox profiling showed that NTP-AW reduced the production of total reactive oxygen species (ROS), and enhanced the antioxidant enzyme activity. Conclusion: Collectively, this study clearly indicates the therapeutic effect of NTP-AW on DNCB-induced AD in hairless mice via immuno-redox regulation, clinically implying that NTP-AW treatment might be a safe and promising therapeutic candidate against AD.

Antimicrobial Activity of Some Schiff Bases Derived from Benzoin, Salicylaldehyde. Aminophenol and 2,4 Dinitrophenyl Hydrazine

Mele Jesmin,M. Mihsin Ali,M. S. Salahuddin,M. Rowshanul Habib,Jahan Ara Khanam 한국균학회 2008 Mycobiology Vol.36 No.1

Hair Growth Promoting Effects of Mineral-rich Spring Water in SKH-1 Hairless Mice

Rahima Begum,Jesmin Ara,Md Faruk Ahmed,Johny Bajgai,Ailyn Fadriquela,Kyu Jae Lee 한국물학회 2017 한국물학회지 Vol.6 No.1

Mineral rich spring water has been used in spa and bathing purpose that often belong to traditional medicinal therapies. It also considered as a classical alternative medicine to cure the immunological disease. Recently, mineral rich containing medicine and cosmetics has been widely applied in hair loss purpose but not tried by mineral water. Therefore, the purpose of the present study is to observe the hair growth promoting effects of mineral rich spring water in SKH-1 hairless mice. We designed this study to evaluate the mineral water has hair growth promoting effect. The experimental SKH-1 hairless mice were divided into a normal control group (NC: No treatment), a positive control group (M: 3% Minoxidil), and different concentrations of mineral water group as a treatment group (MW: 2.5%, 5%, and 10%). All the test samples were topically sprayed on the three distinct parts of the leg, dorsal and a shoulder portion (about 1 cm2 areas) of the mice skin (100 L per application), twice a day, for μ 21 days. The number of hair and length was measured by taking a photograph of using the scale bar tools of the DinoCapture 2.0 digital microscope windows software. The thickness of the hair was measured by using scanning electron microscope. After 21 days of the experiment, mice treated with 2.5% mineral water showed the preferable effect on the number of hair, length, and thickness compared to the PC group and other concentration groups. However, the positive control minoxidil has no mineral containing ingredient but it has strong activity in hair growth. In our study, we found minoxidil had shown less active in hair growth effects in SKH-1 hairless mice. These results suggest that the using of optimum concentration of mineral rich spring water might have a remarked hair growth promoting effect, as observed morphologically. Collectively, according to our studies, we hypothesized that the topical application of mineral rich spring water might represent a novel strategy for the management and therapy for hair falling purpose. However, furthermore, studies could be more assured this hair growth promoting effects.

Potential Therapeutic and Preventive Effect of Drinking Hydrogen-rich Water on Acute Fatigue

Md Faruk Ahmed,Jesmin Ara,AilynFadriquela,RahimaBegum,JohnyBajgai,KyuJaeLee 한국물학회 2017 한국물학회지 Vol.6 No.1

Acute fatigue is a condition defined as a sudden onset of physical and mental exhaustion particularly after a short but strenuous period of physical exercise due to effect of waste product of muscle contraction within muscle fiber and accumulation of metabolites within the muscle fiber. Until recently, it’s believed that lactic acid build-up and reactive oxygen species (ROS) resulting oxidative stress are the most common causes of muscle fatigue. With prevalence of this condition and due to the lack of effective therapeutics, fatigue-related disorders turn to alternative medicine and other non-traditional practices. Previous studies on molecular hydrogen have reported that hydrogen exerts antioxidant, anti-inflammatory and metabolic modulation properties that are beneficial to the cell. Herein we hypothesized that hydrogen-rich water (HW) might ameliorate various impairment of acute fatigue through ROS scavenging activity and elevation of metabolic profile which were related to fatigue such as blood lactate and increasing storage of muscle glycogen, thereby improving physical endurance.

Balneotherapeutic effects of high mineral spring water on the atopic dermatitis-like inflammation in hairless mice via immunomodulation and redox balance

Johny Bajgai,Ailyn Fadriquela,Jesmin Ara,Rahima Begum,Md Faruk Ahmed,Cheol-Su Kim,Soo-Ki Kim,Kwang-Yong Shim,Kyu-Jae Lee 한국물학회 2017 한국물학회지 Vol.6 No.1

Atopic dermatitis (AD) is a chronic relapsing allergic inflammatory skin disease that currently affects millions of children and adults worldwide. Drugs used to treat these inflammatory diseases include anti-histamines, corticosteroids and calcineurin inhibitors but these drugs have their limitations such as adverse effects with their long-term usage. Thus, researcher’s interest in several alternative and complementary therapies are continually growing and balneotherapy is one of these approaches. Therefore, we investigate the bathing effect of high concentration mineral spring water (HMW) on redox balance and immune modulation in 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis like inflammation in hairless mice. We induced AD-like inflammation by application of DNCB on the dorsal skin of female skh-1 hairless mice. The mice were treated with 100% pure HMW (PHMW) and 10% diluted HMW (DHMW) through bathing once a day for 4 weeks. Tacrolimus ointment (0.1%) was used as positive control (PC) and only DNCB treatment as negative control (NeC) group. The severity of skin lesion inflammation was assessed through clinical scoring and observing scratching behavior. Levels of immunoglobulin E (IgE) and inflammatory cytokines in serum were detected by ELISA and multiplex bead array system, and the levels of oxidative stress-related biomarkers and antioxidant enzyme were also measured. We found that HMW significantly decreased the scratching behavior in PHMW and DHMW groups at the 2nd week and in PHMW group at 4th week compared to NeC group. Likewise, serum IgE level was significantly decreased in DHMW group as compared to NeC group. In line, the level of inflammatory cytokines in serum such as interleukin (IL)-1 , IL-13 and tumor necrosis factor- β α were significantly inhibited in PHMW and DHMW groups compared to NeC group. In parallel, total reactive oxygen species (ROS) of serum level was significantly decreased in PHMW treatment groups compared to NeC group. Consistently, serum malondialdehyde (MDA) level in PHMW group was lower than in NeC group. By contrast, glutathione peroxidase (GPx) activity was significantly enhanced in PHMW than NeC. Collectively, our study indicates a balneotherapeutic effect of HMW on DNCB-induced AD like inflammation in hairless mice via immunomodulation and redox balance.

Effects of electrolyzed strong acidic water on wound healing via immune-redox mechanism

Ailyn Fadriquela,Ma Easter Joy Sajo,Johny Bajgai,Jesmin Ara,Soo Ki Kim,Kyu Jae Lee 한국물학회 2017 한국물학회지 Vol.6 No.1

Acidic water is known to inactivate microbes, fungus, viruses, toxins, is used for disinfecting food equipments, vegetables, fruits, poultry and meat. Some studies were also done to show its effect in wound healing. However, the mechanism is not fully understood. This study aimed to unravel the mechanism behind the wound healing effect of strong electrolyzed acidic water (SEAW). The study was conducted to identify the effect of SEAW on cutaneous wounds using a punch removing 5mm skin plug resulting in elliptical wound (Disposable Biopsy Punch, Integra-Miltex, PA, USA). SEAW was generated from a device (HDR Co. Bucheon, Korea), with characteristics of having pH from 5.0 to 6.5, ORP around 800 mV and chlorine concentration of 10 to 30 mg/L. SEAW was sprayed daily for 7 days to the cutaneous wounds of hairless mice and was compared to other treatments such as saline, alcohol and betadine treated groups. Wound areas were measured and digital photographs were conducted daily. Serum and skin lysate were collected on the seventh day, thereafter, reactive oxygen species (ROS) and nitric oxide (NO) assays, oxidative stress assays, and matrix metalloproteinases (MMPs) expressions were analyzed. The results showed that among all groups, SEAW had the highest wound size reduction especially on day 4. ROS in serum was significantly lower than other groups and ROS in skin lysate was lower and comparable to the positive control groups. SEAW has the highest NO activity among all groups which may mean that SEAW can have the most significant action on remodelling stage through the production of NO, and through phagocytosis. Consistently, antioxidant assays of skin lysate shows that GPx, Catalase and MPO activities group treated with SEAW compensate the total ROS in skin. Immunoblotting of matrix metalloproteinases (MMP1 and MMP9), the enzymes responsible for extracellular matrix degradation, were also upregulated in SEAW treated group. Collectively, it is shown that comparable to potent medicine in wound healing such as betadine and alcohol, strong acidic water is similarly effective in wound healing and is shown to act through the immune defense and upregulation of enzymes such as MMPs. However, more studies are still need to be done to confirm these mechanisms.