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MSK1 regulates RANKL-induced NFATc1 expression through CREB and c-Fos
Jeongim Ha, Jung Hye Hwang, Seul Gi Kwon, Da Hye Park, Tae Wan Kim, Deok Gyeong Kang, Kyung Hee Kang, Il-Suk Kim, Chul Wook Kim 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.2
Osteoclasts originated from hematopoietic stem cells are multi-nucleated cells that can resorb the bone matrix. Receptor activator of nuclear factor kappa-B (RANK)/RANK ligand (RANKL) signaling pathway is crucial for the differentiation and activation of osteoclasts. In this study, we investigated for the first time whether or not RANKL induced mitogen- and stress-activated kinase 1 (MSK1) phosphorylation at Ser 376. Activation of MSK1 was detected as soon as 5 min after RANKL stimulation and sparsely detected at 30 min after stimulation. RANKL-induced MSK1 phosphorylation occurred in a dose-dependent manner. MSK1 is known as a downstream signaling molecule of cAMP-dependent protein kinase (PKA). Treatment with the PKA inhibitor H89 significantly suppressed c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) induction upon RANKL stimulation. In addition, cAMP response element-binding protein (CREB) phosphorylation was extremely inhibited by H89 treatment. Mitogen-activated protein kinases (MAPKs) have been investigated for induction of MSK1 phosphorylation. Specific signaling pathway inhibitors for p38 and extracellular signal-regulated kinases (ERKs) significantly blocked RANKL-induced MSK1 activation. Finally, as a downstream effector of the p38-MSK1 pathway, c-Fos transcriptional activity was determined. RANKL-mediated elevation of c-Fos transcriptional activity was significantly suppressed by p38 inhibitor. Moreover, a dominant negative form of CREB suppressed activation of NFATc1. In conclusion, RANKL-stimulated MSK1 phosphorylation could play a role in induction of NFATc1 through CREB and c-Fos activation as a downstream molecule of p38, ERK MAPKs, and PKA. Our results support basic information for the development of osteoclast specific inhibitors.
Developing a Video-based Smart Mastery Learning through Adaptive Evaluation
Jeongim Kang,Moonhee Kim,Seong Baeg Kim 보안공학연구지원센터 2014 International Journal of Software Engineering and Vol.8 No.11
This paper proposes to develop a novel e-learning model based on dynamic formative evaluation. In case of the evaluation of the existing e-learning, repetitive learning to achieve mastery, causes learners to degrade immersion and become neglectful of learning. The dynamic formative evaluation proposed is able to supplement the limitations of the existing approaches. Since a repetitive learning method does not provide perfect feedback, this paper puts an emphasis on dynamic formative evaluation that is able to maximize learning achievement. Through the dynamic formative evaluation, the instructor is able to refer to the evaluation result when making an assessment about the learner. For our model that shows the flow chart of learning, based on dynamic formative evaluation, we prove its effectiveness and validity by implementing our model and analyzing it.
MSK1 regulates RANKL-induced NFATc1 expression through CREB and c-Fos
Jeongim Ha,Jung Hye Hwang,Seul Gi Kwon,Da Hye Park,Tae Wan Kim,Deok Gyeong Kang,Kyung Hee Kang,Chul Wook Kim,Il-Suk Kim 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.2
Osteoclasts originated from hematopoietic stem cells are multi-nucleated cells that can resorb the bone matrix. Receptor activator of nuclear factor kappa-B (RANK)/RANK ligand (RANKL) signaling pathway is crucial for the differentiation and activation of osteoclasts. In this study, we investigated for the first time whether or not RANKL induced mitogen- and stress-activated kinase 1 (MSK1) phosphorylation at Ser 376. Activation of MSK1 was detected as soon as 5 min after RANKL stimulation and sparsely detected at 30 min after stimulation. RANKL-induced MSK1 phosphorylation occurred in a dose-dependent manner. MSK1 is known as a downstream signaling molecule of cAMP-dependent protein kinase (PKA). Treatment with the PKA inhibitor H89 significantly suppressed c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) induction upon RANKL stimulation. In addition, cAMP response element-binding protein (CREB) phosphorylation was extremely inhibited by H89 treatment. Mitogen-activated protein kinases (MAPKs) have been investigated for induction of MSK1 phosphorylation. Specific signaling pathway inhibitors for p38 and extracellular signal-regulated kinases (ERKs) significantly blocked RANKL-induced MSK1 activation. Finally, as a downstream effector of the p38-MSK1 pathway, c-Fos transcriptional activity was determined. RANKL-mediated elevation of c-Fos transcriptional activity was significantly suppressed by p38 inhibitor. Moreover, a dominant negative form of CREB suppressed activation of NFATc1. In conclusion, RANKL-stimulated MSK1 phosphorylation could play a role in induction of NFATc1 through CREB and c-Fos activation as a downstream molecule of p38, ERK MAPKs, and PKA. Our results support basic information for the development of osteoclast specific inhibitors.
Seroprevalence of tuberculosis in domesticated elk (<i>Cervus canadensis</i>) in Korea
Kang, Shin-Seok,Byeon, Hyeon-Seop,Ku, Bok Kyung,Kim, Sang Woo,Kim, Jiro,Woo, Jeongim,Ahn, Byeongwoo,Kim, Soojin,Monoldorova, Sezim,Park, Chan-Ho,Cho, Sang-Nae,Jeon, Bo-Young Elsevier 2016 Research in veterinary science Vol.107 No.-
<P><B>Abstract</B></P> <P>Tuberculosis is a contagious disease in animals, primarily cattle, although it also affects wild animals and humans. There are few data on the state of tuberculosis in domesticated elk (<I>Cervus canadensis</I>) in Korea. In order to investigate tuberculosis in elk, the effectiveness of an enzyme linked immunosorbent assay (ELISA) using MPB70 and MPB83 antigens was compared with the tuberculin skin test (TST), and seroprevalence was measured with this assay using serum samples collected from domesticated elk herds in Korea. The respective sensitivities of the MPB70 and MPB83 ELISAs were 51.9% (95% CI 42.0–61.6) and 49.1% (95% CI 39.3–58.9), and their specificities were 100.0% (95% CI 92.6–100.0) and 97.9% (95% CI 88.9–100.0), respectively, in comparison with the TST. The herd prevalence ranged from 50 to 80% and the mean herd seropositive rate was 67.7% (21 of 31). Of 819 serum samples, 163 (19.9%) were seropositive, and the within-region prevalence ranged from 18.5–58.0%. In conclusion, the ELISA using the MPB70 and MPB83 antigens showed moderate sensitivity and high specificity compared to TST in elk, and tuberculosis was assumed to be fairly prevalent in domesticated elk in Korea.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Establishment of ELISA using the MPB70 and MPB83 antigens for tuberculosis of elk </LI> <LI> Performance of these ELISAs (sensitivity and specificity) compared with TST </LI> <LI> Estimation of seroprevalence of tuberculosis in elk in Korea using these ELISAs </LI> <LI> First report on prevalence data of tuberculosis in domesticated elk in Korea </LI> <LI> A basic epidemiological data of tuberculosis in elk in an Asian region </LI> </UL> </P>
Complement component 9 (C9) 유전자의 단일염기다형성과 버크셔 돼지 육질 형질과의 연관성 분석
하정임(Jeongim Ha),황정혜(Jung Hye Hwang),유고은(Go Eun Yu),박다혜(Da Hye Park),강덕경(Deok Gyeong Kang),김태완(Tae Wan Kim),박화춘(Hwa Chun Park),안상미(Sang Mi An),김철욱(Chul Wook Kim) 한국식품과학회 2018 한국식품과학회지 Vol.50 No.5
본 연구는 Berkshire 간 조직을 이용하여 RNA-sequencing 분석을 통해 돼지 육질 연관 단일염기다형성을 발굴하기 위해 수행되었다. 그 결과, C9 유전자의 cDNA 942번 G 서열이 T 서열로 변환되어 라이신(lysin)이 아스파라진(asparagin)으로 변하는 nonsynonymous SNP를 확인하였다. Berkshire 돼지 405두에서 C9 단일염기다형성의 유전자형을 분석한 결과 major allele는 G, minor allele은 T였다. Berkshire 돼지 405두의 육질 형질을 분석하여 C9 단일염기다형성의 유전자형과 육질 형질과의 연관성 분석한 결과 우성 모델의 경우 육색의 명도, 콜라겐, 수분, 도축 후 24시간 뒤 pH (pH<SUB>24 h</SUB>) 육질 형질에서 유의성이 확인되었고, 열성 모델의 콜라겐 함량, 공우성 모델의 육색의 명도(CIE L), 단백질, 콜라겐 함량에서 유의성을 가졌다. 성별에 따른 C9 유전자형과 육질 형질 간의 연관성을 분석한 결과 거세돈에서 도체중, 콜라겐에서 유의성이 있었으며, 암퇘지의 경우 육색의 명도, 단백질, pH<SUB>24 h</SUB> 육질 형질에서 유의성이 있었다. 육질 형질 중 pH<SUB>24 h</SUB> 형질은 육질을 결정하는 중요한 형질로 C9 유전자의 유전자형이 다른 유전자형들에 비해 pH<SUB>24 h</SUB>가 증가되고 육즙 손실이 감소되는 것으로 확인되어 C9 유전자의 TG 유전자를 가진 돼지가 더 좋은 육질을 가지는 것으로 판단된다. 본 결과를 바탕으로 C9 유전자의 단일염기다형성을 육질을 판단하는 생물마커(biomarker)로 의 활용이 기대된다. In this study, to identify single nucleotide polymorphisms (SNPs) associated with meat quality in Berkshire pigs, we performed RNA sequencing. A non-synonymous SNP (nsSNP) in the Complement component 9 (C9) gene was identified, and the association between meat quality traits and the C9 genotype was analyzed. The nsSNP in the C9 gene was located at c.942 G>T. In the dominant model, significant associations were observed between the SNP and meat quality traits such as CIE L, collagen content, moisture level, and pH<SUB>24 h</SUB>, whereas in the co-dominant model, significant associations were observed between the SNP and CIE L, collagen content, and protein content. In the recessive model, a significant association between the C9 genotype and the collagen content was observed. In addition, we identified the significant relationship between the C9 genotype and meat quality according to sex. These results indicate that the C9 SNP can be used as a genetic marker for improving pork quality.
Effects of LEP, GYS1, MYOD1, and MYF5 polymorphisms on pig economic traits
Park, Sang-Je,Ha, Jeongim,Kim, Il-Suk,Kwon, Seul Gi,Hwang, Jung Hye,Park, Da Hye,Kang, Deok Gyeong,Kim, Tae Wan,Kim, Sam Woong,Kim, Chul Wook Walter de Gruyter GmbH 2015 Annals of animal science Vol.15 No.3
<B>Abstract</B><P> In the present study, we examined the effect of single nucleotide polymorphisms (SNPs) of leptin (LEP), skeletal muscle glycogen synthase (GYS1), myogenic differentiation 1 (MYOD1), and myogenic factor 5 (MYF5) genes on economic trait association in pigs. LEP/HindIII, MYOD1/DdeI, MYF5/FokI, and GYS1/FokI genotypes were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) from 466 pigs comprised of Duroc, Landrace and Yorkshire breeds. The LEP/HindIII polymorphism differed significantly with respect to average daily gain (ADG) in Duroc pigs (P<0.05). However, the GYS1/FokI polymorphism was not significantly associated with any trait. The MYOD1/DdeI polymorphism was significantly associated with both ADG and meat percentage (MP) in Duroc pigs, and ADG, backfat thickness (BFT) and feed efficiency (FE) in Landrace pigs, whereas the MYOD1/DdeI polymorphism was not significantly associated with any trait in Yorkshire pigs. In addition, the MYF5/FokI polymorphism revealed a close relationship with ADG in Duroc pigs. In conclusion, we believe that the SNPs within LEP, MYOD1 and MYF5 in certain pig breeds play important roles as potential genetic markers for economic traits of pigs.</P>
Dong Ju Lee,Jung Hye Hwang,Jeongim Ha,Go Eun Yu,Seulgi Kwon,Da Hye Park,Deok Gyeong Kang,Tae Wan Kim,Hwa Chun Park,Sang Mi An,Chul Wook Kim 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.4
Bromodomain-containing protein 2 (BRD2) is a nuclear serine/threonine kinase involved in transcriptional regulation. We investigated the expression and association of the BRD2 gene as a candidate gene for meat quality traits in Berkshire pigs. BRD2 mRNA was expressed at relatively high levels in muscle tissue. Statistical analysis revealed that the c.1709G>C polymorphism of the BRD2 gene was significantly associated with carcass weight, meat color (a*, redness), protein content, cooking loss, water-holding capacity, carcass temperatures 4, 12 and 24 h postmortem, and the 24 h postmortem pH in 384 Berkshire pigs. Therefore, this polymorphism in the porcine BRD2 gene may be used as a candidate genetic marker to improve meat quality traits in pigs.
Kwon, Seul Gi,Hwang, Jung Hye,Park, Da Hye,Kim, Tae Wan,Kang, Deok Gyeong,Kang, Kyung Hee,Kim, Il-Suk,Ha, Jeongim,Kim, Chul Wook,Plaizier, J. Canadian Science Publishing 2016 Canadian journal of animal science Vol.96 No.1
<P> Corticosteroid-binding globulin (CBG) is a plasma transport protein that has glucocorticoid-binding activity. In the present study, we identified CBG gene expression in several tissues of four pig breeds: Berkshire, Duroc, Landrace, and Yorkshire. Expression of CBG mRNA was detected in the liver of all four breeds, and was the highest in Berkshire pigs. We also found single nucleotide polymorphisms (SNPs) in the CBG gene from Berkshire pigs, including SNP c.919G@@>@@A, which corresponds to 307G@@>@@R. We analyzed the relationships between this CBG variant and various meat-quality traits. The SNP was significantly associated with backfat thickness, post-mortem pH24h, meat color [CIE a* (redness), CIE b* (yellowness)], water-holding capacity, fat content, moisture content, protein content, cooking loss, and shear force. However, the effects differed by gender: the values were significantly associated with almost all traits in gilts, whereas only cooking loss and shear force were shown significantly in barrows. The variant G allele was associated with decreases in backfat thickness, post-mortem pH24h, CIE a*, fat content, and cooking loss, but with increases in CIE b*, water-holding capacity, moisture content, protein content, and shear force. Because the general correlation between meat-quality traits were not validated in the present study, we suggest that certain SNP might be used in the restrictive application to distinguish meat-quality traits. </P>