조건부가치측정법(CVM)을 활용한 개발사업지구 내 유휴부지 유료주차장 활용방안 연구

김태균,박지은,윤정란,Kim, Tae Gyun,Park, Ji Eun,Yun, Jeong Ran 한국토지주택공사 토지주택연구원 2019 LHI journal of land, housing, and urban affairs Vol.10 No.3

In this study, the author suggests utilizing unsold and unused land as temporary commercial parking lots as a way to raise funds for active public transport use. In addition, reasonable parking fee scheme is suggested by estimating marginal parking fee and marginal parking distance for the commercial parking lots based on the CVM. The author conducts a survey to investigate citizens' opinion on utilizing unsold and unused land in the urban development project area as temporary commercial transport facilities such as parking lots. Based on survey outcome, travel behavior and requirements are analyzed and marginal parking fee and marginal parking distance are estimated through the Contingent Valuation Method (CVM). The results are as follows: in the single-bounded dichotomous choice model, people are willing to pay 216 KRW for 165m in average for 10 minutes and their willingness to pay goes down against reaching distance to the parking lots from 365 KRW for 50m, 295 KRW for 100m, 173 KRW for 200m, and 51 KRW for 300m; in the double-bounded dichotomous choice model, people are willing to pay 285 KRW for 165m in average for 10 minutes and their willingness to pay goes down as well against reaching distance to the parking lots from 310 KRW for 50m, 297 KRW for 100m, 272 KRW for 200m and 248 KRW for 300m. It is clearly appeared that people's willingness to pay goes higher as distance to reach the parking lots goes shorter.

Poster Session : PS 1613 ; Mycobacterial Diseases : Serial Evaluation of Humoral Immune Responses to Mycobacterial Antigens in Chronic Tuberculosis Excretors

( Jeong Ran Kim ),( Kyeol Choi ),( Ji Seun Hyun ),( Young Kil Park ),( Hee Jin Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: To understand the function of B cells in immune response of chronic excretors, a serious health problem, in tuberculosis control, we evaluated serological status of chronic excretors during chemotheraphy. Methods: Consecutive hospital treated patients with intractable pulmonary tuberculosis (TB) were evaluated in the antibody responses to mycobacterial antigens such as CFP-10, ESAT-6, Rv3872, HBHA, 16kD, 38kD, MPT64, Ag85B, malate synthase, Rv3878 and MTB48 by Western blot analysis. Results: The same pattern of antibody responses against TB antigens was maintained in intractable pulmonary TB patients for 1 year or 2 years. In comparison between present and past serological antibody responses to the TB antigens, the results were not altered signifi cantly in each chronic excretors during treatment. Conclusions: Our data suggest that chronic excretors consistently produce same kind of antibodies and the humoral responses to TB antigens were not affected by drug treatment.

Clioquinol induces autophagy in cultured astrocytes and neurons by acting as a zinc ionophore

Park, Mi-Ha,Lee, Sook-Jeong,Byun, Hyae-ran,Kim, Yunha,Oh, Young J.,Koh, Jae-Young,Hwang, Jung Jin Elsevier 2011 Neurobiology of disease Vol.42 No.3

<P><B>Abstract</B></P><P>Recent studies have demonstrated that clioquinol, an antibiotic with an anti-amyloid effect, acts as a zinc ionophore under physiological conditions. Because increases in labile zinc may induce autophagy, we examined whether clioquinol induces autophagy in cultured astrocytes in a zinc-dependent manner.</P><P>Within 1h of exposure to 0.1–10μM clioquinol, the levels of microtubule-associated protein 1 light chain 3 (LC3)-II, a marker of autophagy, began to increase in astrocytes. Confocal live-cell imaging of GFP-LC3-transfected astrocytes showed the formation of LC3(+) autophagic vacuoles (AVs), providing a further indication that clioquinol induced autophagy. Addition of 3-methyladenine or small-interfering RNA against autophagy-related gene 6 (ATG6/Beclin-1) blocked clioquinol-induced increases in LC3-II. FluoZin-3 fluorescence microscopy showed that, like the zinc ionophore pyrithione, clioquinol increased intracellular zinc levels in the cytosol and AVs in an extracellular zinc-dependent manner. Zinc chelation with N,N,N′,N′-tetrakis-(2-pyridylmethyl) ethylenediamine (TPEN) reduced, and addition of zinc increased the levels of LC3-II and LC3(+) puncta, indicating that zinc influx plays a key role therein. Moreover, astrocytes and SH-SY5Y cells expressing mutant huntingtin (mHttQ74) accumulated less aggregates when treated with clioquinol, and this effect was reversed by TPEN. These results indicate that clioquinol-induced autophagy is likely to be physiologically functional.</P><P>The present study demonstrates that clioquinol induces autophagy in a zinc-dependent manner and contributes to clearance of aggregated proteins in astrocytes and neurons. Hence, in addition to its metal-chelating effect in and around amyloid beta (Aβ) plaques, clioquinol may contribute to the reduction of Aβ loads by activating autophagy by increasing or normalizing intracellular zinc levels in brain cells.</P> <P><B>Research Highlights</B></P><P>► Clioquinol acts as a zinc ionophore and introduces zinc into autophagic vacuoles. ► Clioquinol induces autophagy in astrocytic cultures in a zinc-dependent manner. ► Autophagy induced by clioquinol reduces accumulation of huntingtin aggregates. ► Excessive clioquinol induces autophagic cell death especially in neurons. ► In conclusion, adequate doses of clioquinol may have beneficial actions in the Alzheimer's Diseases brains.</P>

Short Communication : Rapid determination of sumatriptan in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and its application to clinical pharmacokinetic study

( Jeong Ju Seo ),( Jeonghyeon Park ),( Min Ho Bae ),( Mi Sun Lim ),( Sook Jin Seong ),( Joomi Lee ),( Sung Min Park ),( Hae Won Lee ),( Young Ran Yoon ) 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

A sensitive and simple detection method coupling ultra-performance liquid chromatography with tandem mass spectrometry was developed and validated to analyze sumatriptan levels in humanplasma. The plasma sample preparations for the analysis were based on liquid-liquid extraction with ethyl acetate, evaporation, and reconstitution. MS/MS detection was performed on a triple-quadrupole tandem mass spectrometer by monitoring the protonated parent→daughter ion pairs at m/z 296→58 and m/z 388→71 for sumatriptan and terazosin (internal standard), respectively. The method was validated with respect to its specificity, linearity, sensitivity, accuracy, precision, recovery, and stability. The calibration curve was linear from 0.5 to 50 ng/mL (r>0.999). The mean extraction recovery for sumatriptan was higher than 62.3%. The method accuracy was within 97.4%, and the relative standard deviation of the intra- and inter-day precision values was within 11.7% at all quality control levels. Plasma samples that contained sumatriptan were stable under three freeze-thaw cycles, short- and long-term storage, and autosampler conditions. This method was successfully applied to a pharmacokinetic study conducted with 10 healthy volunteers. After oral administration of 50-mgsumatriptan and serial blood sampling over 12 h, the mean area under the plasma concentration-time curve from time 0 to 12 h and the maximum plasma concentration were 116.2 ng h/mL and 33.2 ng/mL, respectively.ⓒ2013 Elsevier B.B. All rights reserved.

Effective In vitro Propagation from Leaf Segment Culture of Mentha × piperita var. citrata

Jeong-Ae Ko,Jeong-Ran Choi,Young-Sook Kim,Hak-Bong Park,Hyung-Moo Kim,Hyun-Soon Kim 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Clinicopathological Features and Survival of Patients with Gastric Cancer with a Family History: a Large Analysis of 2,736 Patients with Gastric Cancer

Jeong, Oh,Jung, Mi Ran,Park, Young Kyu,Ryu, Seong Yeob The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.2

Purpose: Previous studies indicated conflicting results regarding the prognosis of gastric cancer with a family history (FHX). This study aimed to determine the clinicopathological features and survival of patients with gastric cancer with a FHX. Materials and Methods: We reviewed 2,736 patients with gastric cancer who underwent surgery between 2003 and 2009. The prognostic value of a FHX was determined in the multivariate model after adjusting for variables in the Asian and internationally validated prognostic models. Results: Of the patients, 413 (15.1%) had a FHX of gastric cancer. The patients with a FHX were younger (58.1 vs. 60.4 years; P<0.001) than the patients without a FHX. There were no significant differences in the histopathological characteristics between the 2 groups. A FHX was associated with a better overall survival (OS) rate only in the stage I group (5-year survival rate, 95% vs. 92%; P=0.006). However, the disease-specific survival (DSS) rate was not significantly different between the 2 groups in all stages. The multivariate model adjusted for the variables in the Asian and internationally validated prognostic models revealed that FHX has no significant prognostic value for OS and DSS. Conclusions: The clinicopathological features and survival of the patients with gastric cancer with a FHX did not significantly differ from those of the patients without a FHX.

The Immunosuppressive Potential of Cholesterol Sulfate Through T Cell Microvilli Disruption

Park Jeong-Su,Chung Ik-Joo,Kim Hye-Ran,전창덕 대한면역학회 2023 Immune Network Vol.23 No.3

Cholesterol (CL) is required for various biomolecular production processes, including those of cell membrane components. Therefore, to meet these needs, CL is converted into various derivatives. Among these derivatives is cholesterol sulfate (CS), a naturally produced CL derivative by the sulfotransferase family 2B1 (SULT2B1), which is widely present in human plasma. CS is involved in cell membrane stabilization, blood clotting, keratinocyte differentiation, and TCR nanocluster deformation. This study shows that treatment of T cells with CS resulted in the decreased surface expression of some surface T-cell proteins and reduced IL-2 release. Furthermore, T cells treated with CS significantly reduced lipid raft contents and membrane CLs. Surprisingly, using the electron microscope, we also observed that CS led to the disruption of T-cell microvilli, releasing small microvilli particles containing TCRs and other microvillar proteins. However, in vivo, T cells with CS showed aberrant migration to high endothelial venules and limited infiltrating splenic T-cell zones compared with the untreated T cells. Additionally, we observed significant alleviation of atopic dermatitis in mice injected with CS in the animal model. Based on these results, we conclude that CS is an immunosuppressive natural lipid that impairs TCR signaling by disrupting microvillar function in T cells, suggesting its usefulness as a therapeutic agent for alleviating T-cell-mediated hypersensitivity and a potential target for treating autoimmune diseases.

Asymmetric Synthesis of Novel Pseudo-d-Vinylcyclopropyl Nucleosides Bearing Quaternary Carbon as Potential Anti-Herpesvirus Agent

Park, Ah-Young,Moon, Hyung Ryong,Kim, Kyung Ran,Chun, Moon Woo,Jeong, Lak Shin Taylor Francis 2007 NUCLEOSIDES NUCLEOTIDES AND NUCLEIC ACIDS Vol. No.

<P> Pseudo-d-vinylcyclopropyl nucleosides 10-12 bearing a quaternary carbon were designed and synthesized starting from (R)-epichlorohydrin using a tandem reaction of double alkylation and lactonization via oxirane-ring opening reaction, a Wittig reaction, and chemoselective reduction as potential anti-herpesvirus agent.</P>

Multiple perianal infundibular follicular cysts in a dog

Park, Jin-Kyu,Hong, Il-Hwa,Ki, Mi-Ran,Hong, Kyung-Sook,Ji, Ae-Ri,Do, Sun-Hee,Jeong, Kyu-Shik Blackwell Publishing Ltd 2010 Veterinary dermatology Vol.21 No.3

<P>Abstract</P><P>This case report describes a 7-year-old male cocker spaniel dog with multiple perianal infundibular follicular cysts. Clinically the dog had moderate anal sacculitis, peri-anal pruritus causing it to ‘scoot’ and lick the area. On examination of the perianal area, there were over 100 firm, well circumscribed papules, ranged from 0.2 to 0.5 cm in diameter with a central pore, and were found in the perianal region. Alopecia was present in the perianal region. The skin tissue in the perianal region resected surgically was submitted for histological examination. Microscopically, the tissue revealed multiple dilated cysts filled with keratins and the papules corresponded to infundibular follicular cysts. The affected dog showed moderate anal sacculitis. Anal sacculitis commonly causes repeated scooting or licking the area around the anus. Therefore, the multiple follicular cysts in the present case appear to be primarily a sequela to chronic external trauma to the perianal area, probably in response to anal sacculitis. To the best of the authors’ knowledge, the present report is the first documented case of multiple perianal infundibular follicular cysts in a dog.</P>

Lipopolysaccharide alleviates liver injury induced by thioacetamide in rats

( Jeong Ran Park ),( Eun Jeong Kim ),( Jung Won Yang ),( Han Byeol Lee ),( Seok Ho Hong ),( Seung Koo Lee ),( In Bum Seo ),( Se Min Ryu ),( Sung Joon Cho ),( Heung Myong Woo ),( Sung Min Park ),( Se R 한국예방수의학회 2013 예방수의학회지 Vol.37 No.3

Lipopolysaccharide (LPS) is the major of outer membrane of gram-negative bacteria and one of the most potent microbial initiators of inflammation. From the previous study showed that exposure to a low dose of LPS renders animals tolerant to a lethal dose of LPS, and protects against the toxicity of various chemicals. However, the effects of LPS treatment in thioacetamide (TA) - induced liver injury remain largely unknown. Liver injury caused by various toxic chemicals such as carbon tetrachloride, alcohol, dimethylnitrosamine. Here, we induced rat liver injury by intraperitoneal injection of TA, a representative hepatic fibrosis inducer. In this study, we investigated the effects of LPS in TA group, LPS group, LPS/TA group and vehicle control group on Sprague-Dawely rats (five rats for each group). All rats at the end of the experiment were sacrificed, and liver and serum were obtained. Serological analysis and hematoxylin and eosin staining showed that LPS/TA co-treatment was associated with decrease of aspartate aminotransferase (AST), alanine transaminase (ALT) and totalbilirubin and fibrosis than in TA-treated rats. RT-PCR showed that the levels of IL-6 and Cox2 mRNA were lower in the liver of LPS/TA-cotreated rats than in TA-treated rats. There were no significant differences ALT, ALP, AST, total-bilirubin, IL-6 and Cox2 between vehicle control and LPS-treated rats. These results imply that LPS/TA cotreatment partially alleviates the TA-induced liver injury of rats by reducing inflammatory response.