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Jeong Gyujin,Kim Sanghyun,Nam Bumhee,Lee Chongmu 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.78 No.4
NiO nanoparticle (NP)-functionalized nanograined ZnO nanorods (NRs) were grown by using the thermal evaporation of ZnSe powders; this was followed by the thermal oxidation of the ZnSe NRs to synthesize ZnO NRs and a solvothermal process for NiO functionalization. The diameters of the grains in the synthesized nanograined ZnO NRs are in the range of 50–150 nm. The ZnO grains were not spherical, but they were shaped like rice grains. The diameters and the lengths of the ZnO NRs were in the range of 40–50 nm and 1–6 μm, respectively. The diameters of the NiO NPs were in the range of 40–80 nm. A multiple-networked chemiresistive sensor was fabricated by pouring the IPA solution containing NiO NPdecorated nanograined ZnO NRs onto SiO2/ Si substrates with a patterned electrode. A pristine nanograined ZnO NR sensor was also prepared in a similar manner. The NiO NP-functionalized nanograined ZnO NR sensor exhibited a greater and faster response to H2, as compared to its pristine nanograined ZnO counterpart; it also showed higher selectivity toward H2 against other reducing gases, as compared to the pristine nanograined ZnO counterpart. Herein, the origin of the enhanced sensing performance of the NiO NP-functionalized nanograined ZnO sensor is discussed in detail.
Choi Kyeongbin,Jeong Gyujin,Hyun Soong Keun,Nam Bumhee,Ko Tae Kyung,Lee Chongmu 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.77 No.6
This study reports the effects of NiO nanoparticle (NP) size on the sensing performance of NiO NP-decorated SnO2 nanorods (NRs). NiO NP-decorated SnO2 NRs were synthesized using a two-step process: 1) thermal evaporation of tin powders in an oxidizing atmosphere based on the vapor-liquid-solid growth mechanism and 2) solvothermal decoration of SnO2 NRs with NiO NPs. X-ray diffraction and transmission electron microscopy analyses revealed that both the SnO2 NRs and the NiO NPs were polycrystalline. Scanning electron microscopy images showed that the diameters of the NRs ranged from 100 to 200 nm and that those of the small and the large NiO NPs ranged from 20 to 30 nm and from 80 to 180 nm, respectively. The small NiO NP-decorated SnO2 NRs showed stronger response to NO2 than did the large NiO NP-decorated SnO2 NRs over the concentration range of 0.5 - 100 ppm. Decoration of SnO2 NRs with small NiO NPs resulted in enhanced sensing performance whereas decoration of SnO2 NRs with large NiO NPs deteriorated the sensing performance. The superior NO2 gas sensing performance of the small NiO NP-decorated SnO2 NR sensor as compared to that of the large NiO NP-decorated SnO2 NR sensor was attributed to a higher ratio of n-SnO2 to p-NiO and a higher number of p-n heterojunctions for the same volume of NiO in the former than in the latter. In addition, the small NiO NP-decorated SnO2 NR sensors showed selectivity toward NO2 against other competing gases such as SO2, CO2, CO, H2, C7H8 and C6H6.
Biochemical characterization of type I‑E anti‑CRISPR proteins, AcrIE2 and AcrIE4
Koo Jasung,Lee Gyujin,Ka Donghyun,Park Changkon,Suh Jeong-Yong,Bae Euiyoung 한국응용생명화학회 2023 Applied Biological Chemistry (Appl Biol Chem) Vol.66 No.-
In bacteria and archaea, CRISPRs and Cas proteins constitute an adaptive immune system against invading foreign genetic materials, such as bacteriophages and plasmids. To counteract CRISPR-mediated immunity, bacteriophages encode anti-CRISPR (Acr) proteins that neutralize the host CRISPR–Cas systems. Several Acr proteins that act against type I-E CRISPR–Cas systems have been identified. Here, we describe the biochemical characterization of two type I-E Acr proteins, AcrIE2 and AcrIE4. We determined the crystal structure of AcrIE2 using single-wavelength anomalous diffraction and performed a structural comparison with the previously reported AcrIE2 structures solved by different techniques. Binding assays with type I-E Cas proteins were carried out for the target identification of AcrIE2. We also analyzed the interaction between AcrIE4 and its target Cas component using biochemical methods. Our findings corroborate and expand the knowledge on type I-E Acr proteins, illuminating diverse molecular mechanisms of inhibiting CRISPR-mediated prokaryotic anti-phage defense.
Lee, Ji-Young,Kim, Bong Jo,Sim, Gyujin,Kim, Gyu-Tae,Kang, Dawon,Jung, Jae Hun,Hwa, Jeong Seok,Kwak, Yeon Ju,Choi, Yeon Jin,Park, Young Sook,Han, Jaehee,Lee, Cheol Soon,Kang, Kee Ryeon The Korean Academy of Medical Sciences 2011 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.26 No.6
<P>The influence of spinal cord injury (SCI) on protein expression in the rat urinary bladder was assessed by proteomic analysis at different time intervals post-injury. After contusion SCI between T9 and T10, bladder tissues were processed by 2-DE and MALDI-TOF/MS at 6 hr to 28 days after SCI to identify proteins involved in the healing process of SCI-induced neurogenic bladder. Approximately 1,000 spots from the bladder of SCI and sham groups were visualized and identified. At one day after SCI, the expression levels of three protein were increased, and seven spots were down-regulated, including heat shock protein 27 (Hsp27) and heat shock protein 20 (Hsp20). Fifteen spots such as S100-A11 were differentially expressed seven days post-injury, and seven proteins including transgelin had altered expression patterns 28 days after injury. Of the proteins with altered expression levels, transgelin, S100-A11, Hsp27 and Hsp20 were continuously and variably expressed throughout the entire post-SCI recovery of the bladder. The identified proteins at each time point belong to eight functional categories. The altered expression patterns identified by 2-DE of transgelin and S100-A11 were verified by Western blot. Transgelin and protein S100-A11 may be candidates for protein biomarkers in the bladder healing process after SCI.</P>
구영우(Youngwoo Ku),노영희(Younghee Rho),정규진(Gyujin Jeong),민완기(Wankee Min) 한국기술혁신학회 2013 기술혁신학회지 Vol.16 No.3
본 연구는 혁신체제론에 입각해서 국내 생명공학산업의 조직간 혁신네트워크 현황 및 활성화방안을 실증적으로 분석했다. 본 연구의 결과를 요약하면, 다음과 같다. 아직 국내 생명공학산업의 혁신네트워크는 활성화되지 못했지만 혁신네트워크에 참여하고 있는 벤처기업 근무자, 대학교수, 공공연구기관 연구원들은 혁신 네트워크 및 혁신네트워크의 성과에 대하여 긍정적으로 평가하고 있다. 이는 국내 생명공학산업의 혁신네트워크가 활성화될 필요성이 있음을 의미한다. 실증분석 결과 혁신네트워크에 참여하는 주체들의 의지와 능력이 제고되고, 혁신네트워크 내 참여주체간 신뢰와 통제가 강화되고, 혁신네트워크내 참여주체들의 기술접근과 자원확보가 잘 이루어진다면, 혁신네트워크는 더욱 활성화될 수 있을 것이다. This study analyses interorganizational innovation networks empirically in the Korean biotechnology industry, based on systems of innovation approach. Empirical findings of this study are as follows. Innovation networks have not been well developed in the Korean biotechnology industry. Main agents who participate in innovation networks are workers at venture firms, university professors, researchers at government-sponsored research institutions. They all recognize the positive effects of innovation networks. The enhancement of participation willingness and competency of agents, the enrichment of trust and control among agents, and the effective acquisition of the useful technology and resources in innovation networks will largely contribute to development of innovation networks in the Korean biotechnology industry.